Rh5AG397800

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
Physical Location & Seq
Reverse (-)
68900618 .. 68903621
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG397800.1

Sequence Viewer

Length: 1002 bp
ATGGCATCCATGGCTTCCACACTCGCTGCCTCCTCTCTCTCCACCAAGCTCCAGAAGCCATCTCTCCTCGAGTCCTCCTTCCACGGCGTTCCCGTTGCTCGGGTTCAGCCTTTGAAGGCGGTCTCATCCAACGGCCACGGCCTCTCCGTGTCCATGTCCGCTGGTGGCTCCACCAGCGCTTACGATTTGAGCAACTTCAAGTTCGAGTCGATCAAGGAGTCCATCGTCTCCCGAGAGATGACCAGGCGATACATGACTGACATGATCACCTACGCCGATACCGACGTTGTCATCGTCGGTGCCGGTTCCGCAGGGCTATCTTGCGCATGGGAGCTCAGCAAGAACCCCGATGTGCAGGTAGCCATTATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCGTGGCTCGGTGGCCAGCTCTTCTCCGCCATGGTAGTGAGGAAACCAGCACATCACTTCCTCAACGAGTTGGGCATTGACTACGACGAGAAGGATGACTACGTAGTCATTAAGCATGCTGCCCTATTCACCTCAACTATCATGAGCAAGCTCCTTGCCCGTCCCAATGTCAAACTCTTCAACGCCGTAGCAGCTGAGGATTTGATCATCAAGGGTGGAAGAGTTGCTGGTGTTGTCACCAACTGGGCCTTGGTCTCCATGAACCACGACACACAGTCGTGCATGGACCCCAACGTTATGGAGTCCAAGGTTGTGGTAAGCTCATGCGGTCACGATGGGCCAATGGGAGCCACTGGAGTAAAGAGGTTGAGGAGTGTTGGGATGATTGAGAGCGTGCCTGGGATGAAGGCCTTGGACATGAATGCAGCAGAGGATGCCATTGTGAAGCTAACAAGGGAAATAGTGCCTGGAATGATCGTGACTGGCATGGAAGTTGCTGAGATTGATGGCTCTCCAAGAATGGTAAATTTTTATATAATAAATTTATTCTTTATGCTTCAATATATGTTCTCTTATGTTTCTTCCCAAATGACTTATTTTGAATAG

Protein Analysis

333

Amino Acids

35.85

Weight (kDa)

5.83

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 77 - 307 4.5e-110 Thi4 family
DAO PF01266 95 - 142 3.2e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 95 - 131 5.6e-06 FAD binding domain
NAD_binding_8 PF13450 98 - 133 3.2e-07 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 500
Acc16I TGCGCA 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 346
AccB1I GGYRCC 1 cut(s) 299
AccII CGCG 1 cut(s) 398
AciI CCGC 7 cut(s) 119, 159, 309, 390, 393, 423, 723
AclI AACGTT 1 cut(s) 690
AcoI YGGCCR 2 cut(s) 133, 409
AcsI RAATTY 2 cut(s) 922, 937
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 2 cut(s) 99, 392
AgsI TTSAA 5 cut(s) 115, 199, 577, 956, 998
AhdI GACNNNNNGTC 1 cut(s) 670
AjnI CCWGG 3 cut(s) 242, 793, 862
AleI CACNNNNGTG 1 cut(s) 673
AluBI AGCT 7 cut(s) 49, 334, 415, 547, 590, 717, 844
AluI AGCT 7 cut(s) 49, 334, 415, 547, 590, 717, 844
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 3 cut(s) 127, 232, 655
Ama87I CYCGRG 3 cut(s) 68, 99, 231
Aor51HI AGCGCT 1 cut(s) 178
AoxI GGCC 6 cut(s) 133, 139, 409, 642, 734, 804
ApeKI GCWGC 4 cut(s) 26, 515, 587, 821
ApoI RAATTY 2 cut(s) 922, 937
AspLEI GCGC 3 cut(s) 179, 326, 398
AspS9I GGNCC 3 cut(s) 642, 682, 734
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 3 cut(s) 259, 517, 625
AvaI CYCGRG 3 cut(s) 68, 99, 231
AvaII GGWCC 1 cut(s) 682
BaeI ACNNNNGTAYC 2 cut(s) 270, 303
BalI TGGCCA 1 cut(s) 411
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 336
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
Bbv12I GWGCWC 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 4 cut(s) 13, 502, 599, 833
BccI CCATC 4 cut(s) 67, 230, 725, 896
BceAI ACGGC 4 cut(s) 100, 148, 154, 566
BciT130I CCWGG 3 cut(s) 244, 795, 864
BclI TGATCA 2 cut(s) 264, 600
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 3 cut(s) 127, 232, 655
BfoI RGCGCY 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 390
BisI GCNGC 6 cut(s) 27, 391, 394, 516, 588, 822
BlpI GCTNAGC 1 cut(s) 335
BlsI GCNGC 6 cut(s) 28, 392, 395, 517, 589, 823
Bme1390I CCNGG 4 cut(s) 244, 387, 795, 864
Bme18I GGWCC 1 cut(s) 682
BmeRI GACNNNNNGTC 1 cut(s) 670
BmeT110I CYCGRG 3 cut(s) 68, 99, 231
BmgT120I GGNCC 3 cut(s) 642, 682, 734
BmiI GGNNCC 5 cut(s) 169, 301, 307, 684, 745
BmrFI CCNGG 4 cut(s) 244, 387, 795, 864
BmrI ACTGGG 1 cut(s) 649
BmsI GCATC 2 cut(s) 14, 820
BmuI ACTGGG 1 cut(s) 649
BpmI CTGGAG 2 cut(s) 35, 771
Bpu10I CCTNAGC 1 cut(s) 591
Bpu1102I GCTNAGC 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 499
BsaI GGTCTC 2 cut(s) 127, 655
BsaJI CCNNGG 9 cut(s) 9, 82, 136, 385, 426, 645, 702, 794, 807
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 392
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 644, 754, 883
BseBI CCWGG 3 cut(s) 244, 795, 864
BseDI CCNNGG 9 cut(s) 9, 82, 136, 385, 426, 645, 702, 794, 807
BseGI GGATG 6 cut(s) 5, 125, 496, 783, 804, 835
BseLI CCNNNNNNNGG 2 cut(s) 99, 392
BseMII CTCAG 3 cut(s) 349, 582, 885
BseNI ACTGG 3 cut(s) 644, 754, 883
BseRI GAGGAG 3 cut(s) 22, 56, 781
BseXI GCAGC 4 cut(s) 13, 502, 599, 833
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 6 cut(s) 135, 141, 411, 644, 736, 806
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 3 cut(s) 68, 99, 231
BsiSI CCGG 2 cut(s) 303, 387
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 99, 392
BsmAI GTCTC 3 cut(s) 127, 232, 655
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 6 cut(s) 135, 141, 411, 644, 736, 806
Bso31I GGTCTC 2 cut(s) 127, 655
BsoBI CYCGRG 3 cut(s) 68, 99, 231
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 4 cut(s) 210, 264, 600, 870
Bsp1720I GCTNAGC 1 cut(s) 335
Bsp19I CCATGG 2 cut(s) 9, 426
BspACI CCGC 7 cut(s) 119, 159, 309, 390, 393, 423, 723
BspANI GGCC 6 cut(s) 135, 141, 411, 644, 736, 806
BspCNI CTCAG 3 cut(s) 348, 583, 886
BspFNI CGCG 1 cut(s) 398
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 5 cut(s) 169, 301, 307, 684, 745
BspMI ACCTGC 1 cut(s) 346
BspQI GCTCTTC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 127, 655
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 3 cut(s) 644, 754, 883
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 9 cut(s) 9, 82, 136, 385, 426, 645, 702, 794, 807
BssMI GATC 4 cut(s) 210, 264, 600, 870
BssT1I CCWWGG 5 cut(s) 9, 426, 645, 702, 807
Bst2UI CCWGG 3 cut(s) 244, 795, 864
Bst4CI ACNGT 1 cut(s) 672
Bst6I CTCTTC 3 cut(s) 422, 578, 610
BstAPI GCANNNNNTGC 1 cut(s) 830
BstBAI YACGTR 1 cut(s) 499
BstC8I GCNNGC 4 cut(s) 413, 513, 545, 791
BstDEI CTNAG 3 cut(s) 335, 591, 894
BstDSI CCRYGG 4 cut(s) 9, 82, 136, 426
BstF5I GGATG 6 cut(s) 5, 125, 496, 783, 804, 835
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 3 cut(s) 179, 326, 398
BstKTI GATC 4 cut(s) 213, 267, 603, 873
BstMAI GTCTC 3 cut(s) 127, 232, 655
BstMBI GATC 4 cut(s) 210, 264, 600, 870
BstMWI GCNNNNNNNGC 7 cut(s) 11, 55, 174, 308, 390, 587, 830
BstNI CCWGG 3 cut(s) 244, 795, 864
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 4 cut(s) 242, 385, 793, 862
BstSNI TACGTA 1 cut(s) 499
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 4 cut(s) 13, 502, 599, 833
BstXI CCANNNNNNTGG 2 cut(s) 694, 709
BsuRI GGCC 6 cut(s) 135, 141, 411, 644, 736, 806
BtgI CCRYGG 4 cut(s) 9, 82, 136, 426
BtsCI GGATG 6 cut(s) 5, 125, 496, 783, 804, 835
BtsIMutI CAGTG 1 cut(s) 747
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 413, 513, 545, 791
CciI TCATGA 1 cut(s) 537
CfoI GCGC 3 cut(s) 179, 326, 398
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 642, 682, 734
DdeI CTNAG 3 cut(s) 335, 591, 894
DpnI GATC 4 cut(s) 212, 266, 602, 872
DpnII GATC 4 cut(s) 210, 264, 600, 870
DrdI GACNNNNNNGTC 1 cut(s) 500
DriI GACNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 2 cut(s) 133, 409
Eam1104I CTCTTC 3 cut(s) 422, 578, 610
Eam1105I GACNNNNNGTC 1 cut(s) 670
EarI CTCTTC 3 cut(s) 422, 578, 610
EciI GGCGGA 1 cut(s) 412
Ecl136II GAGCTC 1 cut(s) 334
Eco105I TACGTA 1 cut(s) 499
Eco130I CCWWGG 5 cut(s) 9, 426, 645, 702, 807
Eco147I AGGCCT 1 cut(s) 806
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 127, 655
Eco47I GGWCC 1 cut(s) 682
Eco47III AGCGCT 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 334
Eco88I CYCGRG 3 cut(s) 68, 99, 231
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 242, 793, 862
EcoT14I CCWWGG 5 cut(s) 9, 426, 645, 702, 807
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 5 cut(s) 9, 426, 645, 702, 807
Esp3I CGTCTC 1 cut(s) 232
FaqI GGGAC 1 cut(s) 543
FbaI TGATCA 2 cut(s) 264, 600
Fnu4HI GCNGC 6 cut(s) 27, 391, 394, 516, 588, 822
FokI GGATG 5 cut(s) 112, 503, 790, 811, 842
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 6 cut(s) 27, 391, 394, 516, 588, 822
FspI TGCGCA 1 cut(s) 325
GlaI GCGC 3 cut(s) 178, 325, 397
GluI GCNGC 6 cut(s) 27, 391, 394, 516, 588, 822
GsuI CTGGAG 2 cut(s) 35, 771
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 6 cut(s) 135, 141, 411, 644, 736, 806
HapII CCGG 2 cut(s) 303, 387
HhaI GCGC 3 cut(s) 179, 326, 398
Hin6I GCGC 3 cut(s) 177, 324, 396
HinP1I GCGC 3 cut(s) 177, 324, 396
HinfI GANTC 4 cut(s) 71, 206, 218, 698
HpaII CCGG 2 cut(s) 303, 387
HphI GGTGA 3 cut(s) 259, 517, 625
Hpy188III TCNNGA 5 cut(s) 52, 231, 538, 728, 874
Hpy99I CGWCG 3 cut(s) 287, 299, 485
HpyAV CCTTC 4 cut(s) 88, 109, 481, 796
HpyCH4III ACNGT 1 cut(s) 672
HpyCH4IV ACGT 3 cut(s) 285, 498, 690
HpyCH4V TGCA 3 cut(s) 355, 678, 821
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 55, 174, 308, 390, 587, 830
HpyF3I CTNAG 3 cut(s) 335, 591, 894
HpySE526I ACGT 3 cut(s) 285, 498, 690
HspAI GCGC 3 cut(s) 177, 324, 396
Ksp22I TGATCA 2 cut(s) 264, 600
Kzo9I GATC 4 cut(s) 210, 264, 600, 870
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 5 cut(s) 54, 173, 331, 552, 743
Lsp1109I GCAGC 4 cut(s) 13, 502, 599, 833
LweI GCATC 2 cut(s) 14, 820
MaeII ACGT 3 cut(s) 285, 498, 690
MaeIII GTNAC 3 cut(s) 631, 725, 874
MalI GATC 4 cut(s) 212, 266, 602, 872
MboI GATC 4 cut(s) 210, 264, 600, 870
MboII GAAGA 4 cut(s) 409, 565, 627, 969
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 2 cut(s) 922, 937
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 4 cut(s) 80, 215, 227, 707
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 1 cut(s) 411
MscI TGGCCA 1 cut(s) 411
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 2 cut(s) 431, 673
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 161, 590
MspI CCGG 2 cut(s) 303, 387
MspR9I CCNGG 4 cut(s) 244, 387, 795, 864
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 3 cut(s) 244, 795, 864
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 7 cut(s) 11, 55, 174, 308, 390, 587, 830
NciI CCSGG 1 cut(s) 387
NcoI CCATGG 2 cut(s) 9, 426
NdeII GATC 4 cut(s) 210, 264, 600, 870
NlaIV GGNNCC 5 cut(s) 169, 301, 307, 684, 745
NmuCI GTSAC 3 cut(s) 631, 725, 874
NsbI TGCGCA 1 cut(s) 325
NspI RCATGY 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 673
PaeI GCATGC 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 68
PagI TCATGA 1 cut(s) 537
PceI AGGCCT 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 4 cut(s) 90, 144, 279, 291
PctI GAATGC 1 cut(s) 823
PflFI GACNNNGTC 1 cut(s) 287
PkrI GCNGC 6 cut(s) 28, 392, 395, 517, 589, 823
PleI GAGTC 4 cut(s) 79, 214, 226, 706
PpsI GAGTC 4 cut(s) 79, 214, 226, 706
Ppu21I YACGTR 1 cut(s) 499
Psp124BI GAGCTC 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 242, 793, 862
PspGI CCWGG 3 cut(s) 242, 793, 862
PspN4I GGNNCC 5 cut(s) 169, 301, 307, 684, 745
PspPI GGNCC 3 cut(s) 642, 682, 734
PspXI VCTCGAGB 1 cut(s) 68
PsyI GACNNNGTC 1 cut(s) 287
PvuII CAGCTG 1 cut(s) 590
RseI CAYNNNNRTG 2 cut(s) 431, 673
SacI GAGCTC 1 cut(s) 336
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 6 cut(s) 27, 391, 394, 516, 588, 822
Sau3AI GATC 4 cut(s) 210, 264, 600, 870
Sau96I GGNCC 3 cut(s) 642, 682, 734
SchI GAGTC 4 cut(s) 80, 215, 227, 707
ScrFI CCNGG 4 cut(s) 244, 387, 795, 864
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 14, 820
Sfr274I CTCGAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 682
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 2 cut(s) 431, 673
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SnaBI TACGTA 1 cut(s) 499
SphI GCATGC 1 cut(s) 515
Sse9I AATT 2 cut(s) 922, 937
SseBI AGGCCT 1 cut(s) 806
SsiI CCGC 7 cut(s) 119, 159, 309, 390, 393, 423, 723
SstI GAGCTC 1 cut(s) 336
StuI AGGCCT 1 cut(s) 806
StyD4I CCNGG 4 cut(s) 242, 385, 793, 862
StyI CCWWGG 5 cut(s) 9, 426, 645, 702, 807
TaaI ACNGT 1 cut(s) 672
TaiI ACGT 3 cut(s) 288, 501, 693
TaqI TCGA 3 cut(s) 69, 204, 209
TasI AATT 2 cut(s) 922, 937
TauI GCSGC 2 cut(s) 393, 396
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 3 cut(s) 631, 725, 874
TseI GCWGC 4 cut(s) 26, 515, 587, 821
Tsp45I GTSAC 3 cut(s) 631, 725, 874
TspDTI ATGAA 3 cut(s) 671, 815, 830
TspGWI ACGGA 2 cut(s) 136, 367
TspRI CASTG 1 cut(s) 754
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 2 cut(s) 922, 937
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XhoI CTCGAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.