Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G54770 |
| fragaria_vesca | FvH4_3g21940 FvH4_3g33490 |
| malus_domestica | MD03G1210100.v1.1 MD11G1224800.v1.1 |
| prunus_persica | Prupe.4G205100_v2.0.a1 |
| pyrus_communis | pycom03g15900 |
| rosa_chinensis | RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911 |
| rosa_laevigata | RLG00000033809 RLG00000035410 |
| rosa_multiflora | Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00019770 Rroxscaffold_1G00043140 |
| rosa_rugosa | Rorug05G0167100 Rorug05G0334800 |
| rosa_samantha | Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000 |
| rosa_wichuraiana | Rw5G023770 Rw5G037510 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 48, 220 |
| AclWI | GGATC | 1 cut(s) | 261 |
| AcsI | RAATTY | 2 cut(s) | 51, 127 |
| AfaI | GTAC | 1 cut(s) | 63 |
| AflIII | ACRYGT | 1 cut(s) | 10 |
| AgsI | TTSAA | 1 cut(s) | 324 |
| AluBI | AGCT | 1 cut(s) | 318 |
| AluI | AGCT | 1 cut(s) | 318 |
| Alw21I | GWGCWC | 1 cut(s) | 22 |
| Alw26I | GTCTC | 1 cut(s) | 302 |
| Alw44I | GTGCAC | 1 cut(s) | 18 |
| AlwI | GGATC | 1 cut(s) | 261 |
| AoxI | GGCC | 1 cut(s) | 223 |
| ApaLI | GTGCAC | 1 cut(s) | 18 |
| ApoI | RAATTY | 2 cut(s) | 51, 127 |
| AspS9I | GGNCC | 2 cut(s) | 80, 223 |
| AvaII | GGWCC | 1 cut(s) | 80 |
| BaeGI | GKGCMC | 1 cut(s) | 22 |
| Bbv12I | GWGCWC | 1 cut(s) | 22 |
| BccI | CCATC | 1 cut(s) | 242 |
| BciVI | GTATCC | 1 cut(s) | 276 |
| BcoDI | GTCTC | 1 cut(s) | 302 |
| BfaI | CTAG | 1 cut(s) | 122 |
| BfmI | CTRYAG | 1 cut(s) | 132 |
| BfuI | GTATCC | 1 cut(s) | 276 |
| BmcAI | AGTACT | 1 cut(s) | 63 |
| Bme18I | GGWCC | 1 cut(s) | 80 |
| BmgT120I | GGNCC | 2 cut(s) | 80, 223 |
| BmiI | GGNNCC | 1 cut(s) | 224 |
| Bsa29I | ATCGAT | 2 cut(s) | 246, 297 |
| BsaAI | YACGTR | 1 cut(s) | 11 |
| BsaBI | GATNNNNATC | 1 cut(s) | 252 |
| BsaJI | CCNNGG | 1 cut(s) | 278 |
| Bse8I | GATNNNNATC | 1 cut(s) | 252 |
| BseCI | ATCGAT | 2 cut(s) | 246, 297 |
| BseDI | CCNNGG | 1 cut(s) | 278 |
| BseJI | GATNNNNATC | 1 cut(s) | 252 |
| BseSI | GKGCMC | 1 cut(s) | 22 |
| Bsh1285I | CGRYCG | 1 cut(s) | 301 |
| BshFI | GGCC | 1 cut(s) | 225 |
| BshVI | ATCGAT | 2 cut(s) | 246, 297 |
| BsiEI | CGRYCG | 1 cut(s) | 301 |
| BsiHKAI | GWGCWC | 1 cut(s) | 22 |
| BsmAI | GTCTC | 1 cut(s) | 302 |
| BsnI | GGCC | 1 cut(s) | 225 |
| Bsp1286I | GDGCHC | 1 cut(s) | 22 |
| Bsp143I | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| BspACI | CCGC | 2 cut(s) | 48, 220 |
| BspANI | GGCC | 1 cut(s) | 225 |
| BspDI | ATCGAT | 2 cut(s) | 246, 297 |
| BspLI | GGNNCC | 1 cut(s) | 224 |
| BspMAI | CTGCAG | 1 cut(s) | 136 |
| BspPI | GGATC | 1 cut(s) | 261 |
| BssECI | CCNNGG | 1 cut(s) | 278 |
| BssMI | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| BssT1I | CCWWGG | 1 cut(s) | 278 |
| Bst4CI | ACNGT | 1 cut(s) | 205 |
| BstBAI | YACGTR | 1 cut(s) | 11 |
| BstKTI | GATC | 5 cut(s) | 156, 246, 256, 297, 301 |
| BstMAI | GTCTC | 1 cut(s) | 302 |
| BstMBI | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| BstMCI | CGRYCG | 1 cut(s) | 301 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 148 |
| BstNSI | RCATGY | 2 cut(s) | 116, 220 |
| BstSFI | CTRYAG | 1 cut(s) | 132 |
| BstSLI | GKGCMC | 1 cut(s) | 22 |
| Bsu15I | ATCGAT | 2 cut(s) | 246, 297 |
| BsuI | GTATCC | 1 cut(s) | 276 |
| BsuRI | GGCC | 1 cut(s) | 225 |
| BsuTUI | ATCGAT | 2 cut(s) | 246, 297 |
| Cfr13I | GGNCC | 2 cut(s) | 80, 223 |
| ClaI | ATCGAT | 2 cut(s) | 246, 297 |
| Csp6I | GTAC | 1 cut(s) | 62 |
| CviAII | CATG | 2 cut(s) | 113, 217 |
| CviJI | RGCY | 5 cut(s) | 5, 142, 151, 225, 318 |
| CviKI_1 | RGCY | 5 cut(s) | 5, 142, 151, 225, 318 |
| CviQI | GTAC | 1 cut(s) | 62 |
| DpnI | GATC | 5 cut(s) | 155, 245, 255, 296, 300 |
| DpnII | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| Eco130I | CCWWGG | 1 cut(s) | 278 |
| Eco47I | GGWCC | 1 cut(s) | 80 |
| EcoRI | GAATTC | 1 cut(s) | 51 |
| EcoT14I | CCWWGG | 1 cut(s) | 278 |
| ErhI | CCWWGG | 1 cut(s) | 278 |
| FaeI | CATG | 2 cut(s) | 116, 220 |
| FaiI | YATR | 8 cut(s) | 8, 57, 114, 147, 190, 218, 264, 306 |
| FatI | CATG | 2 cut(s) | 112, 216 |
| FauI | CCCGC | 1 cut(s) | 213 |
| FspBI | CTAG | 1 cut(s) | 122 |
| HaeIII | GGCC | 1 cut(s) | 225 |
| Hin1II | CATG | 2 cut(s) | 116, 220 |
| HincII | GTYRAC | 1 cut(s) | 165 |
| HindII | GTYRAC | 1 cut(s) | 165 |
| HindIII | AAGCTT | 1 cut(s) | 316 |
| Hpy166II | GTNNAC | 2 cut(s) | 20, 165 |
| Hpy188I | TCNGA | 1 cut(s) | 172 |
| Hpy8I | GTNNAC | 2 cut(s) | 20, 165 |
| HpyAV | CCTTC | 2 cut(s) | 67, 71 |
| HpyCH4III | ACNGT | 1 cut(s) | 205 |
| HpyCH4IV | ACGT | 2 cut(s) | 10, 192 |
| HpyCH4V | TGCA | 2 cut(s) | 20, 134 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 148 |
| HpySE526I | ACGT | 2 cut(s) | 10, 192 |
| Hsp92II | CATG | 2 cut(s) | 116, 220 |
| Kzo9I | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| LpnPI | CCDG | 3 cut(s) | 94, 120, 272 |
| MaeI | CTAG | 1 cut(s) | 122 |
| MaeII | ACGT | 2 cut(s) | 10, 192 |
| MaeIII | GTNAC | 1 cut(s) | 205 |
| MalI | GATC | 5 cut(s) | 155, 245, 255, 296, 300 |
| MboI | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| MboII | GAAGA | 1 cut(s) | 185 |
| MhlI | GDGCHC | 1 cut(s) | 22 |
| MluCI | AATT | 3 cut(s) | 51, 127, 257 |
| MmeI | TCCRAC | 1 cut(s) | 175 |
| MnlI | CCTC | 1 cut(s) | 132 |
| MseI | TTAA | 1 cut(s) | 234 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 148 |
| NdeII | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| NlaIII | CATG | 2 cut(s) | 116, 220 |
| NlaIV | GGNNCC | 1 cut(s) | 224 |
| NmuCI | GTSAC | 1 cut(s) | 205 |
| NspI | RCATGY | 2 cut(s) | 116, 220 |
| Ple19I | CGATCG | 1 cut(s) | 301 |
| Ppu21I | YACGTR | 1 cut(s) | 11 |
| PspN4I | GGNNCC | 1 cut(s) | 224 |
| PspPI | GGNCC | 2 cut(s) | 80, 223 |
| PstI | CTGCAG | 1 cut(s) | 136 |
| PvuI | CGATCG | 1 cut(s) | 301 |
| RsaI | GTAC | 1 cut(s) | 63 |
| RsaNI | GTAC | 1 cut(s) | 62 |
| SaqAI | TTAA | 1 cut(s) | 234 |
| Sau3AI | GATC | 5 cut(s) | 153, 243, 253, 294, 298 |
| Sau96I | GGNCC | 2 cut(s) | 80, 223 |
| ScaI | AGTACT | 1 cut(s) | 63 |
| SduI | GDGCHC | 1 cut(s) | 22 |
| SetI | ASST | 4 cut(s) | 13, 82, 195, 320 |
| SfcI | CTRYAG | 1 cut(s) | 132 |
| SinI | GGWCC | 1 cut(s) | 80 |
| Sse9I | AATT | 3 cut(s) | 51, 127, 257 |
| SsiI | CCGC | 2 cut(s) | 48, 220 |
| SspMI | CTAG | 1 cut(s) | 122 |
| StyI | CCWWGG | 1 cut(s) | 278 |
| TaaI | ACNGT | 1 cut(s) | 205 |
| TaiI | ACGT | 2 cut(s) | 13, 195 |
| TaqI | TCGA | 2 cut(s) | 246, 297 |
| TasI | AATT | 3 cut(s) | 51, 127, 257 |
| TatI | WGTACW | 1 cut(s) | 61 |
| Tru1I | TTAA | 1 cut(s) | 234 |
| Tru9I | TTAA | 1 cut(s) | 234 |
| TseFI | GTSAC | 1 cut(s) | 205 |
| Tsp45I | GTSAC | 1 cut(s) | 205 |
| TspDTI | ATGAA | 1 cut(s) | 44 |
| VneI | GTGCAC | 1 cut(s) | 18 |
| VpaK11BI | GGWCC | 1 cut(s) | 80 |
| XapI | RAATTY | 2 cut(s) | 51, 127 |
| XceI | RCATGY | 2 cut(s) | 116, 220 |
| XspI | CTAG | 1 cut(s) | 122 |
| ZrmI | AGTACT | 1 cut(s) | 63 |