Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Forward (+)

32369311 .. 32372941

Gene Structure 3631 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG258700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGGCATCCATGGCTTCCACACTTGGCGCCTCATCGCTCTCCGCCAAGCTCCAGAAGCCATCTCTCCTCGACTCCTCCTTCCACGGCGCTCCCGTCGCCCGCGTCCAGCCACTCAAGGCCGCCTCATCCAACGGCCACGGCCTCTCCGTCTCCATGTCCGCCGGCGCCTCCACCAACTCCTATGATCTCAGCAACTTCAAGTTCGAGCCGATCAAGGAGTCCATCGTCTCCAGGGAGATGACCAGGCGCTACATGACTGACATGATCACCTACGCCGATACCGACGTCGTCGTCGTCGGTGCTGGCTCCGCCGGGCTTTCCTGCGCCTACGAGCTCAGCAAGAACCCCGATGTGCAGGTTGCTATTATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCGTGGCTCGGTGGCCAGCTTTTCTCCGCCATGGTTGTGAGGAAACCAGCACATCACTTCCTTAACGAGTTGGGCATTGCCTACGACGAGGCGGATGACTATGTAGTCATCAAGCATGCTGCTCTATTCACCTCAACTATTATGAGCAAGCTCCTTGCTCGGCCCAATGTGAAACTCTTCAACGCCGTAGCAGCTGAGGATTTGATCATCAAGGGTGGGAGAGTTGGTGGTGTTGTCACCAACTGGGCCTTGGTCTCCATGAACCATGACACACAGTCGTGCATGGACCCCAACGTTATGGAAGCCAAGGTGGTGGTTAGCTCATGTGGTCATGATGGCCCAATGGGAGCCACCGGCGTGAAGAGGTTGAGGAGCGTTGGGATGATTGACAGCGTGCCTGGGATGAAGGCCTTGGACATGAATGCAGCTGAAGACGCCATTGTGAAGCTAACCAGGGAAATAGTGCCTGGAATGATTGTTACTGGCATGGAAGTTGCTGAGATTGATGGATCTCCAAGAATGGTAAATTTCTTCTCATGGATTTTTTATCTTTTATTTATTTGGTTCTTTTTTGTTTTTTTCCTATCTGTTTCCTCTTTTGTGAAAAATTTGGTTATCTTTCGAGAAGAGTTAGAACCAAAATTAGGAAATAAGGAGGAATGTTTGCATGAATTGTTAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

359

Amino Acids

38.72

Weight (kDa)

5.78

Isoelectric Point (pI)

36.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	77 - 310	2.5e-110	Thi4 family
FAD_binding_2	PF00890	95 - 131	9.5e-07	FAD binding domain
DAO	PF01266	95 - 142	2.9e-08	FAD dependent oxidoreductase
Pyr_redox_2	PF07992	95 - 136	7.9e-06	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	98 - 133	6.4e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	290, 500
AatII	GACGTC	1 cut(s)	288
Acc36I	ACCTGC	1 cut(s)	346
AccB1I	GGYRCC	2 cut(s)	26, 164
AccII	CGCG	2 cut(s)	102, 398
AciI	CCGC	9 cut(s)	42, 100, 120, 159, 309, 390, 393, 423, 488
AclI	AACGTT	1 cut(s)	690
AclWI	GGATC	1 cut(s)	913
AcoI	YGGCCR	2 cut(s)	133, 409
AcsI	RAATTY	2 cut(s)	922, 1003
AcuI	CTGAAG	1 cut(s)	846
AcyI	GRCGYC	4 cut(s)	27, 165, 285, 831
AfiI	CCNNNNNNNGG	2 cut(s)	392, 1040
AgsI	TTSAA	2 cut(s)	199, 577
AhdI	GACNNNNNGTC	1 cut(s)	670
AjnI	CCWGG	5 cut(s)	230, 242, 793, 848, 862
AleI	CACNNNNGTG	2 cut(s)	673, 752
AluBI	AGCT	8 cut(s)	49, 334, 415, 547, 590, 717, 824, 844
AluI	AGCT	8 cut(s)	49, 334, 415, 547, 590, 717, 824, 844
Alw21I	GWGCWC	1 cut(s)	336
Alw26I	GTCTC	3 cut(s)	154, 232, 655
AlwI	GGATC	1 cut(s)	913
AoxI	GGCC	8 cut(s)	117, 133, 139, 409, 557, 642, 733, 804
ApeKI	GCWGC	3 cut(s)	515, 587, 821
ApoI	RAATTY	2 cut(s)	922, 1003
Asp700I	GAANNNNTTC	1 cut(s)	572
AspLEI	GCGC	6 cut(s)	29, 89, 167, 249, 326, 398
AspS9I	GGNCC	4 cut(s)	558, 642, 682, 734
AsuC2I	CCSGG	2 cut(s)	313, 387
AsuHPI	GGTGA	3 cut(s)	259, 517, 625
AvaII	GGWCC	1 cut(s)	682
BaeI	ACNNNNGTAYC	2 cut(s)	270, 303
BalI	TGGCCA	1 cut(s)	411
BanI	GGYRCC	2 cut(s)	26, 164
BanII	GRGCYC	1 cut(s)	336
BbsI	GAAGAC	1 cut(s)	834
Bbv12I	GWGCWC	1 cut(s)	336
BbvCI	CCTCAGC	1 cut(s)	591
BbvI	GCAGC	3 cut(s)	502, 599, 833
BccI	CCATC	4 cut(s)	67, 230, 725, 896
BceAI	ACGGC	4 cut(s)	100, 148, 154, 566
BciT130I	CCWGG	5 cut(s)	232, 244, 795, 850, 864
BclI	TGATCA	2 cut(s)	264, 600
BcnI	CCSGG	2 cut(s)	313, 387
BcoDI	GTCTC	3 cut(s)	154, 232, 655
BfoI	RGCGCY	4 cut(s)	30, 90, 168, 250
BfuAI	ACCTGC	1 cut(s)	346
BglI	GCCNNNNNGGC	1 cut(s)	390
BisI	GCNGC	6 cut(s)	120, 391, 394, 516, 588, 822
BlpI	GCTNAGC	1 cut(s)	335
BlsI	GCNGC	6 cut(s)	121, 392, 395, 517, 589, 823
Bme1390I	CCNGG	7 cut(s)	232, 244, 313, 387, 795, 850, 864
Bme18I	GGWCC	1 cut(s)	682
BmeRI	GACNNNNNGTC	1 cut(s)	670
BmgT120I	GGNCC	4 cut(s)	558, 642, 682, 734
BmiI	GGNNCC	5 cut(s)	28, 166, 307, 684, 745
BmrFI	CCNGG	7 cut(s)	232, 244, 313, 387, 795, 850, 864
BmrI	ACTGGG	1 cut(s)	649
BmsI	GCATC	1 cut(s)	14
BmuI	ACTGGG	1 cut(s)	649
BpiI	GAAGAC	1 cut(s)	834
BpmI	CTGGAG	2 cut(s)	35, 214
Bpu10I	CCTNAGC	1 cut(s)	591
Bpu1102I	GCTNAGC	1 cut(s)	335
BpuEI	CTTGAG	1 cut(s)	98
BpuMI	CCSGG	2 cut(s)	313, 387
BsaHI	GRCGYC	4 cut(s)	27, 165, 285, 831
BsaI	GGTCTC	1 cut(s)	655
BsaXI	ACNNNNNCTCC	4 cut(s)	128, 158, 607, 637
Bsc4I	CCNNNNNNNGG	2 cut(s)	392, 1040
Bse118I	RCCGGY	2 cut(s)	161, 749
Bse1I	ACTGG	2 cut(s)	644, 883
Bse3DI	GCAATG	1 cut(s)	471
BseBI	CCWGG	5 cut(s)	232, 244, 795, 850, 864
BseGI	GGATG	5 cut(s)	5, 125, 496, 783, 804
BseLI	CCNNNNNNNGG	2 cut(s)	392, 1040
BseMI	GCAATG	1 cut(s)	471
BseMII	CTCAG	4 cut(s)	202, 349, 582, 885
BseNI	ACTGG	2 cut(s)	644, 883
BseRI	GAGGAG	3 cut(s)	56, 64, 781
BseXI	GCAGC	3 cut(s)	502, 599, 833
BsgI	GTGCAG	1 cut(s)	374
Bsh1236I	CGCG	2 cut(s)	102, 398
BshFI	GGCC	8 cut(s)	119, 135, 141, 411, 559, 644, 735, 806
BshNI	GGYRCC	2 cut(s)	26, 164
BsiHKAI	GWGCWC	1 cut(s)	336
BsiSI	CCGG	4 cut(s)	162, 312, 387, 750
BslI	CCNNNNNNNGG	2 cut(s)	392, 1040
BsmAI	GTCTC	3 cut(s)	154, 232, 655
BsmBI	CGTCTC	2 cut(s)	154, 232
BsmI	GAATGC	1 cut(s)	823
BsnI	GGCC	8 cut(s)	119, 135, 141, 411, 559, 644, 735, 806
Bso31I	GGTCTC	1 cut(s)	655
Bsp1286I	GDGCHC	1 cut(s)	336
Bsp143I	GATC	5 cut(s)	184, 210, 264, 600, 905
Bsp1720I	GCTNAGC	1 cut(s)	335
Bsp19I	CCATGG	2 cut(s)	9, 426
BspACI	CCGC	9 cut(s)	42, 100, 120, 159, 309, 390, 393, 423, 488
BspANI	GGCC	8 cut(s)	119, 135, 141, 411, 559, 644, 735, 806
BspCNI	CTCAG	4 cut(s)	201, 348, 583, 886
BspFNI	CGCG	2 cut(s)	102, 398
BspHI	TCATGA	1 cut(s)	727
BspLI	GGNNCC	5 cut(s)	28, 166, 307, 684, 745
BspMI	ACCTGC	1 cut(s)	346
BspPI	GGATC	1 cut(s)	913
BspT107I	GGYRCC	2 cut(s)	26, 164
BspTNI	GGTCTC	1 cut(s)	655
BsrDI	GCAATG	1 cut(s)	471
BsrFI	RCCGGY	2 cut(s)	161, 749
BsrI	ACTGG	2 cut(s)	644, 883
BssAI	RCCGGY	2 cut(s)	161, 749
BssMI	GATC	5 cut(s)	184, 210, 264, 600, 905
BssNI	GRCGYC	4 cut(s)	27, 165, 285, 831
BssT1I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Bst2UI	CCWGG	5 cut(s)	232, 244, 795, 850, 864
Bst4CI	ACNGT	1 cut(s)	672
Bst6I	CTCTTC	3 cut(s)	578, 752, 1017
BstACI	GRCGYC	4 cut(s)	27, 165, 285, 831
BstC8I	GCNNGC	7 cut(s)	100, 163, 304, 413, 513, 545, 791
BstDEI	CTNAG	4 cut(s)	188, 335, 591, 894
BstDSI	CCRYGG	4 cut(s)	9, 82, 136, 426
BstF5I	GGATG	5 cut(s)	5, 125, 496, 783, 804
BstFNI	CGCG	2 cut(s)	102, 398
BstH2I	RGCGCY	4 cut(s)	30, 90, 168, 250
BstHHI	GCGC	6 cut(s)	29, 89, 167, 249, 326, 398
BstKTI	GATC	5 cut(s)	187, 213, 267, 603, 908
BstMAI	GTCTC	3 cut(s)	154, 232, 655
BstMBI	GATC	5 cut(s)	184, 210, 264, 600, 905
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 55, 95, 308, 390, 587, 830
BstNI	CCWGG	5 cut(s)	232, 244, 795, 850, 864
BstNSI	RCATGY	1 cut(s)	515
BstSCI	CCNGG	7 cut(s)	230, 242, 311, 385, 793, 848, 862
BstUI	CGCG	2 cut(s)	102, 398
BstV1I	GCAGC	3 cut(s)	502, 599, 833
BstV2I	GAAGAC	1 cut(s)	834
BstX2I	RGATCY	1 cut(s)	905
BstXI	CCANNNNNNTGG	2 cut(s)	694, 709
BstYI	RGATCY	1 cut(s)	905
BsuRI	GGCC	8 cut(s)	119, 135, 141, 411, 559, 644, 735, 806
BtgI	CCRYGG	4 cut(s)	9, 82, 136, 426
BtgZI	GCGATG	1 cut(s)	18
BtsCI	GGATG	5 cut(s)	5, 125, 496, 783, 804
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	7 cut(s)	100, 163, 304, 413, 513, 545, 791
CciI	TCATGA	1 cut(s)	727
CfoI	GCGC	6 cut(s)	29, 89, 167, 249, 326, 398
Cfr10I	RCCGGY	2 cut(s)	161, 749
Cfr13I	GGNCC	4 cut(s)	558, 642, 682, 734
CseI	GACGC	2 cut(s)	91, 839
DdeI	CTNAG	4 cut(s)	188, 335, 591, 894
DinI	GGCGCC	2 cut(s)	28, 166
DpnI	GATC	5 cut(s)	186, 212, 266, 602, 907
DpnII	GATC	5 cut(s)	184, 210, 264, 600, 905
DrdI	GACNNNNNNGTC	2 cut(s)	290, 500
DriI	GACNNNNNGTC	1 cut(s)	670
DseDI	GACNNNNNNGTC	2 cut(s)	290, 500
EaeI	YGGCCR	2 cut(s)	133, 409
Eam1104I	CTCTTC	3 cut(s)	578, 752, 1017
Eam1105I	GACNNNNNGTC	1 cut(s)	670
EarI	CTCTTC	3 cut(s)	578, 752, 1017
EciI	GGCGGA	5 cut(s)	31, 148, 298, 412, 503
Ecl136II	GAGCTC	1 cut(s)	334
Eco130I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Eco147I	AGGCCT	1 cut(s)	806
Eco24I	GRGCYC	1 cut(s)	336
Eco31I	GGTCTC	1 cut(s)	655
Eco47I	GGWCC	1 cut(s)	682
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	1 cut(s)	846
EcoICRI	GAGCTC	1 cut(s)	334
EcoRII	CCWGG	5 cut(s)	230, 242, 793, 848, 862
EcoT14I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
EcoT38I	GRGCYC	1 cut(s)	336
EgeI	GGCGCC	2 cut(s)	28, 166
EheI	GGCGCC	2 cut(s)	28, 166
ErhI	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Esp3I	CGTCTC	2 cut(s)	154, 232
FauI	CCCGC	1 cut(s)	107
FbaI	TGATCA	2 cut(s)	264, 600
Fnu4HI	GCNGC	6 cut(s)	120, 391, 394, 516, 588, 822
FokI	GGATG	4 cut(s)	112, 503, 790, 811
FriOI	GRGCYC	1 cut(s)	336
Fsp4HI	GCNGC	6 cut(s)	120, 391, 394, 516, 588, 822
GlaI	GCGC	6 cut(s)	28, 88, 166, 248, 325, 397
GluI	GCNGC	6 cut(s)	120, 391, 394, 516, 588, 822
GsuI	CTGGAG	2 cut(s)	35, 214
HaeII	RGCGCY	4 cut(s)	30, 90, 168, 250
HaeIII	GGCC	8 cut(s)	119, 135, 141, 411, 559, 644, 735, 806
HapII	CCGG	4 cut(s)	162, 312, 387, 750
HgaI	GACGC	2 cut(s)	91, 839
HhaI	GCGC	6 cut(s)	29, 89, 167, 249, 326, 398
Hin1I	GRCGYC	4 cut(s)	27, 165, 285, 831
Hin6I	GCGC	6 cut(s)	27, 87, 165, 247, 324, 396
HinP1I	GCGC	6 cut(s)	27, 87, 165, 247, 324, 396
HinfI	GANTC	2 cut(s)	71, 218
HpaII	CCGG	4 cut(s)	162, 312, 387, 750
HphI	GGTGA	3 cut(s)	259, 517, 625
Hpy188III	TCNNGA	3 cut(s)	52, 728, 1019
Hpy99I	CGWCG	7 cut(s)	98, 287, 290, 293, 296, 299, 485
HpyAV	CCTTC	2 cut(s)	88, 796
HpyCH4III	ACNGT	1 cut(s)	672
HpyCH4IV	ACGT	2 cut(s)	285, 690
HpyCH4V	TGCA	4 cut(s)	355, 678, 821, 1063
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 55, 95, 308, 390, 587, 830
HpyF3I	CTNAG	4 cut(s)	188, 335, 591, 894
HpySE526I	ACGT	2 cut(s)	285, 690
Hsp92I	GRCGYC	4 cut(s)	27, 165, 285, 831
HspAI	GCGC	6 cut(s)	27, 87, 165, 247, 324, 396
KasI	GGCGCC	2 cut(s)	26, 164
KroI	GCCGGC	1 cut(s)	161
KroNI	GCCGGC	1 cut(s)	163
Ksp22I	TGATCA	2 cut(s)	264, 600
Kzo9I	GATC	5 cut(s)	184, 210, 264, 600, 905
LmnI	GCTCC	6 cut(s)	54, 94, 311, 552, 743, 768
Lsp1109I	GCAGC	3 cut(s)	502, 599, 833
LweI	GCATC	1 cut(s)	14
MaeII	ACGT	2 cut(s)	285, 690
MaeIII	GTNAC	2 cut(s)	631, 874
MalI	GATC	5 cut(s)	186, 212, 266, 602, 907
MboI	GATC	5 cut(s)	184, 210, 264, 600, 905
MboII	GAAGA	5 cut(s)	565, 769, 839, 919, 1034
MflI	RGATCY	1 cut(s)	905
MhlI	GDGCHC	1 cut(s)	336
MlsI	TGGCCA	1 cut(s)	411
MluCI	AATT	4 cut(s)	922, 1003, 1037, 1067
MluNI	TGGCCA	1 cut(s)	411
Mly113I	GGCGCC	2 cut(s)	27, 165
MlyI	GAGTC	2 cut(s)	65, 227
MmeI	TCCRAC	1 cut(s)	153
Mox20I	TGGCCA	1 cut(s)	411
MreI	CGCCGGCG	1 cut(s)	161
MroNI	GCCGGC	1 cut(s)	161
MroXI	GAANNNNTTC	1 cut(s)	572
MscI	TGGCCA	1 cut(s)	411
MseI	TTAA	1 cut(s)	459
MslI	CAYNNNNRTG	3 cut(s)	431, 673, 752
Msp20I	TGGCCA	1 cut(s)	411
MspA1I	CMGCKG	2 cut(s)	590, 824
MspI	CCGG	4 cut(s)	162, 312, 387, 750
MspR9I	CCNGG	7 cut(s)	232, 244, 313, 387, 795, 850, 864
Mva1269I	GAATGC	1 cut(s)	823
MvaI	CCWGG	5 cut(s)	232, 244, 795, 850, 864
MvnI	CGCG	2 cut(s)	102, 398
MwoI	GCNNNNNNNGC	7 cut(s)	11, 55, 95, 308, 390, 587, 830
NaeI	GCCGGC	1 cut(s)	163
NarI	GGCGCC	2 cut(s)	27, 165
NciI	CCSGG	2 cut(s)	313, 387
NcoI	CCATGG	2 cut(s)	9, 426
NdeII	GATC	5 cut(s)	184, 210, 264, 600, 905
NgoMIV	GCCGGC	1 cut(s)	161
NlaIV	GGNNCC	5 cut(s)	28, 166, 307, 684, 745
NmeAIII	GCCGAG	1 cut(s)	535
NmuCI	GTSAC	1 cut(s)	631
NspI	RCATGY	1 cut(s)	515
OliI	CACNNNNGTG	2 cut(s)	673, 752
PaeI	GCATGC	1 cut(s)	515
PagI	TCATGA	1 cut(s)	727
PceI	AGGCCT	1 cut(s)	806
PcsI	WCGNNNNNNNCGW	4 cut(s)	90, 144, 279, 291
PctI	GAATGC	1 cut(s)	823
PdiI	GCCGGC	1 cut(s)	163
PdmI	GAANNNNTTC	1 cut(s)	572
PflFI	GACNNNGTC	1 cut(s)	287
PkrI	GCNGC	6 cut(s)	121, 392, 395, 517, 589, 823
PleI	GAGTC	2 cut(s)	65, 226
PluTI	GGCGCC	2 cut(s)	30, 168
PpsI	GAGTC	2 cut(s)	65, 226
Psp124BI	GAGCTC	1 cut(s)	336
Psp1406I	AACGTT	1 cut(s)	690
Psp6I	CCWGG	5 cut(s)	230, 242, 793, 848, 862
PspGI	CCWGG	5 cut(s)	230, 242, 793, 848, 862
PspN4I	GGNNCC	5 cut(s)	28, 166, 307, 684, 745
PspPI	GGNCC	4 cut(s)	558, 642, 682, 734
PsuI	RGATCY	1 cut(s)	905
PsyI	GACNNNGTC	1 cut(s)	287
PvuII	CAGCTG	2 cut(s)	590, 824
RseI	CAYNNNNRTG	3 cut(s)	431, 673, 752
SacI	GAGCTC	1 cut(s)	336
SaqAI	TTAA	1 cut(s)	459
SatI	GCNGC	6 cut(s)	120, 391, 394, 516, 588, 822
Sau3AI	GATC	5 cut(s)	184, 210, 264, 600, 905
Sau96I	GGNCC	4 cut(s)	558, 642, 682, 734
SchI	GAGTC	2 cut(s)	65, 227
ScrFI	CCNGG	7 cut(s)	232, 244, 313, 387, 795, 850, 864
SduI	GDGCHC	1 cut(s)	336
SfaNI	GCATC	1 cut(s)	14
SfoI	GGCGCC	2 cut(s)	28, 166
SgrAI	CRCCGGYG	2 cut(s)	161, 749
SinI	GGWCC	1 cut(s)	682
SmiMI	CAYNNNNRTG	3 cut(s)	431, 673, 752
SmlI	CTYRAG	1 cut(s)	113
SmoI	CTYRAG	1 cut(s)	113
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	4 cut(s)	922, 1003, 1037, 1067
SseBI	AGGCCT	1 cut(s)	806
SsiI	CCGC	9 cut(s)	42, 100, 120, 159, 309, 390, 393, 423, 488
SspDI	GGCGCC	2 cut(s)	26, 164
SstI	GAGCTC	1 cut(s)	336
StuI	AGGCCT	1 cut(s)	806
StyD4I	CCNGG	7 cut(s)	230, 242, 311, 385, 793, 848, 862
StyI	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
TaaI	ACNGT	1 cut(s)	672
TaiI	ACGT	2 cut(s)	288, 693
TaqI	TCGA	3 cut(s)	69, 204, 1018
TasI	AATT	4 cut(s)	922, 1003, 1037, 1067
TauI	GCSGC	3 cut(s)	122, 393, 396
Tru1I	TTAA	1 cut(s)	459
Tru9I	TTAA	1 cut(s)	459
TseFI	GTSAC	1 cut(s)	631
TseI	GCWGC	3 cut(s)	515, 587, 821
Tsp45I	GTSAC	1 cut(s)	631
TspDTI	ATGAA	4 cut(s)	671, 815, 830, 1080
TspGWI	ACGGA	2 cut(s)	136, 367
Tth111I	GACNNNGTC	1 cut(s)	287
VpaK11BI	GGWCC	1 cut(s)	682
XapI	RAATTY	2 cut(s)	922, 1003
XceI	RCATGY	1 cut(s)	515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	643
XmnI	GAANNNNTTC	1 cut(s)	572
ZraI	GACGTC	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.