Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Reverse (-)

17137863 .. 17139235

Gene Structure 1373 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g15900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 609 bp

ATGGCAACCATGGCTTCCACCCTCACCTCCTCCTCCCTCACCGCCAAGCTCCAGAAGTCCTCCCTCCTCGACTCCTCCTTCCATGGCGCTCCCATGGTCCGCCTTCAGCCGGCCAAGGCGGCCCCCGCCAACAACGGCGGCCTTTCCGTCTCCATGTCTGCCAACGGCTTGACCCCCTCCTACGACTTGAGCGCTTTCAAGTTCGACCCCATCAAGGAATCCATCGTCTCCCGGGAGATGACCAGGCGCTACATGACTGACATGATCACCTACGCCGACACAGACGTCGTTGTCGTCGGTGCTGGCTCCGCCGGGCTATCCTGCGCCTACGAACTCAGCAAGAACCCCGACGTTCAGGTGGCCATCATCGAGCAGTCCGTCAGCCCCGGTGGAGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCCATGGTTGTGCGCAAACCCGCCCATCTCTTCCTTAACGAGCTCGGCATTGACTACGATGAGCAAGACAACTACGTTGTCATCAAGCACGCCGCCCTATTCACCTCCACCATCATGAGCAAGCTCCTTGCCCGGCCCAACGTGAAGCTCTTCAACGCTGTGGCTGCCGAGGACTTGATCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

203

Amino Acids

21.51

Weight (kDa)

5.59

Isoelectric Point (pI)

45.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	77 - 201	7.9e-60	Thi4 family
Pyr_redox_2	PF07992	94 - 136	4.6e-06	Pyridine nucleotide-disulphide oxidoreductase
DAO	PF01266	95 - 143	7.2e-09	FAD dependent oxidoreductase
FAD_binding_2	PF00890	95 - 131	2.9e-07	FAD binding domain
NAD_binding_8	PF13450	98 - 152	2.1e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	284, 290
AatII	GACGTC	1 cut(s)	288
Acc16I	TGCGCA	1 cut(s)	437
AciI	CCGC	8 cut(s)	42, 100, 119, 126, 138, 309, 444, 516
AcoI	YGGCCR	3 cut(s)	111, 360, 409
AcuI	CTGAAG	1 cut(s)	89
AcyI	GRCGYC	1 cut(s)	285
AfeI	AGCGCT	1 cut(s)	193
AfiI	CCNNNNNNNGG	3 cut(s)	109, 214, 392
AgsI	TTSAA	2 cut(s)	199, 577
AjnI	CCWGG	1 cut(s)	242
AluBI	AGCT	5 cut(s)	49, 415, 466, 547, 571
AluI	AGCT	5 cut(s)	49, 415, 466, 547, 571
Alw21I	GWGCWC	1 cut(s)	468
Alw26I	GTCTC	2 cut(s)	154, 232
Ama87I	CYCGRG	1 cut(s)	231
Aor51HI	AGCGCT	1 cut(s)	193
AoxI	GGCC	6 cut(s)	111, 120, 139, 360, 409, 557
ApeKI	GCWGC	1 cut(s)	587
Asp700I	GAANNNNTTC	1 cut(s)	572
AspLEI	GCGC	5 cut(s)	89, 194, 249, 326, 438
AspS9I	GGNCC	3 cut(s)	97, 121, 558
AsuC2I	CCSGG	5 cut(s)	232, 233, 313, 387, 556
AsuHPI	GGTGA	4 cut(s)	16, 31, 259, 517
AvaI	CYCGRG	1 cut(s)	231
AvaII	GGWCC	1 cut(s)	97
BalI	TGGCCA	2 cut(s)	362, 411
BanII	GRGCYC	1 cut(s)	468
Bbv12I	GWGCWC	1 cut(s)	468
BbvI	GCAGC	1 cut(s)	574
BccI	CCATC	5 cut(s)	218, 230, 371, 456, 542
BceAI	ACGGC	2 cut(s)	151, 181
BciT130I	CCWGG	1 cut(s)	244
BclI	TGATCA	1 cut(s)	264
BcnI	CCSGG	5 cut(s)	232, 233, 313, 387, 556
BcoDI	GTCTC	2 cut(s)	154, 232
BfoI	RGCGCY	3 cut(s)	90, 195, 250
BglI	GCCNNNNNGGC	1 cut(s)	119
BisI	GCNGC	4 cut(s)	120, 139, 516, 588
BlsI	GCNGC	4 cut(s)	121, 140, 517, 589
Bme1390I	CCNGG	6 cut(s)	232, 233, 244, 313, 387, 556
Bme18I	GGWCC	1 cut(s)	97
BmeT110I	CYCGRG	1 cut(s)	231
BmgT120I	GGNCC	3 cut(s)	97, 121, 558
BmiI	GGNNCC	2 cut(s)	123, 307
BmrFI	CCNGG	6 cut(s)	232, 233, 244, 313, 387, 556
BpmI	CTGGAG	1 cut(s)	35
BpuEI	CTTGAG	1 cut(s)	208
BpuMI	CCSGG	5 cut(s)	232, 233, 313, 387, 556
Bsa29I	ATCGAT	1 cut(s)	603
BsaHI	GRCGYC	1 cut(s)	285
BsaJI	CCNNGG	8 cut(s)	9, 82, 93, 114, 231, 385, 426, 591
Bsc4I	CCNNNNNNNGG	3 cut(s)	109, 214, 392
Bse118I	RCCGGY	1 cut(s)	109
BseBI	CCWGG	1 cut(s)	244
BseCI	ATCGAT	1 cut(s)	603
BseDI	CCNNGG	8 cut(s)	9, 82, 93, 114, 231, 385, 426, 591
BseLI	CCNNNNNNNGG	3 cut(s)	109, 214, 392
BseMII	CTCAG	1 cut(s)	349
BseRI	GAGGAG	4 cut(s)	19, 22, 56, 64
BseXI	GCAGC	1 cut(s)	574
BshFI	GGCC	6 cut(s)	113, 122, 141, 362, 411, 559
BshVI	ATCGAT	1 cut(s)	603
BsiHKAI	GWGCWC	1 cut(s)	468
BsiHKCI	CYCGRG	1 cut(s)	231
BsiSI	CCGG	5 cut(s)	110, 232, 312, 387, 556
BslI	CCNNNNNNNGG	3 cut(s)	109, 214, 392
BsmAI	GTCTC	2 cut(s)	154, 232
BsmBI	CGTCTC	2 cut(s)	154, 232
BsnI	GGCC	6 cut(s)	113, 122, 141, 362, 411, 559
BsoBI	CYCGRG	1 cut(s)	231
Bsp1286I	GDGCHC	1 cut(s)	468
Bsp143I	GATC	2 cut(s)	264, 600
Bsp19I	CCATGG	4 cut(s)	9, 82, 93, 426
BspACI	CCGC	8 cut(s)	42, 100, 119, 126, 138, 309, 444, 516
BspANI	GGCC	6 cut(s)	113, 122, 141, 362, 411, 559
BspCNI	CTCAG	1 cut(s)	348
BspDI	ATCGAT	1 cut(s)	603
BspHI	TCATGA	1 cut(s)	537
BspLI	GGNNCC	2 cut(s)	123, 307
BspQI	GCTCTTC	2 cut(s)	422, 578
BsrFI	RCCGGY	1 cut(s)	109
BssAI	RCCGGY	1 cut(s)	109
BssECI	CCNNGG	8 cut(s)	9, 82, 93, 114, 231, 385, 426, 591
BssMI	GATC	2 cut(s)	264, 600
BssNI	GRCGYC	1 cut(s)	285
BssT1I	CCWWGG	5 cut(s)	9, 82, 93, 114, 426
Bst2UI	CCWGG	1 cut(s)	244
Bst6I	CTCTTC	3 cut(s)	422, 458, 578
BstACI	GRCGYC	1 cut(s)	285
BstC8I	GCNNGC	5 cut(s)	111, 304, 413, 513, 545
BstDEI	CTNAG	1 cut(s)	335
BstDSI	CCRYGG	4 cut(s)	9, 82, 93, 426
BstH2I	RGCGCY	3 cut(s)	90, 195, 250
BstHHI	GCGC	5 cut(s)	89, 194, 249, 326, 438
BstKTI	GATC	2 cut(s)	267, 603
BstMAI	GTCTC	2 cut(s)	154, 232
BstMBI	GATC	2 cut(s)	264, 600
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 119, 125, 308, 587
BstNI	CCWGG	1 cut(s)	244
BstSCI	CCNGG	6 cut(s)	230, 231, 242, 311, 385, 554
BstV1I	GCAGC	1 cut(s)	574
Bsu15I	ATCGAT	1 cut(s)	603
BsuRI	GGCC	6 cut(s)	113, 122, 141, 362, 411, 559
BsuTUI	ATCGAT	1 cut(s)	603
BtgI	CCRYGG	4 cut(s)	9, 82, 93, 426
Cac8I	GCNNGC	5 cut(s)	111, 304, 413, 513, 545
CciI	TCATGA	1 cut(s)	537
CfoI	GCGC	5 cut(s)	89, 194, 249, 326, 438
Cfr10I	RCCGGY	1 cut(s)	109
Cfr13I	GGNCC	3 cut(s)	97, 121, 558
Cfr9I	CCCGGG	1 cut(s)	231
ClaI	ATCGAT	1 cut(s)	603
CviAII	CATG	9 cut(s)	10, 83, 94, 154, 253, 262, 399, 427, 538
DdeI	CTNAG	1 cut(s)	335
DpnI	GATC	2 cut(s)	266, 602
DpnII	GATC	2 cut(s)	264, 600
DrdI	GACNNNNNNGTC	2 cut(s)	284, 290
DseDI	GACNNNNNNGTC	2 cut(s)	284, 290
EaeI	YGGCCR	3 cut(s)	111, 360, 409
Eam1104I	CTCTTC	3 cut(s)	422, 458, 578
EarI	CTCTTC	3 cut(s)	422, 458, 578
EciI	GGCGGA	2 cut(s)	89, 298
Ecl136II	GAGCTC	1 cut(s)	466
Eco130I	CCWWGG	5 cut(s)	9, 82, 93, 114, 426
Eco24I	GRGCYC	1 cut(s)	468
Eco47I	GGWCC	1 cut(s)	97
Eco47III	AGCGCT	1 cut(s)	193
Eco53kI	GAGCTC	1 cut(s)	466
Eco57I	CTGAAG	1 cut(s)	89
Eco88I	CYCGRG	1 cut(s)	231
EcoICRI	GAGCTC	1 cut(s)	466
EcoRII	CCWGG	1 cut(s)	242
EcoT14I	CCWWGG	5 cut(s)	9, 82, 93, 114, 426
EcoT38I	GRGCYC	1 cut(s)	468
ErhI	CCWWGG	5 cut(s)	9, 82, 93, 114, 426
Esp3I	CGTCTC	2 cut(s)	154, 232
FaeI	CATG	9 cut(s)	13, 86, 97, 157, 256, 265, 402, 430, 541
FaiI	YATR	9 cut(s)	11, 84, 95, 155, 254, 263, 400, 428, 539
FatI	CATG	9 cut(s)	9, 82, 93, 153, 252, 261, 398, 426, 537
FauI	CCCGC	2 cut(s)	133, 451
FbaI	TGATCA	1 cut(s)	264
Fnu4HI	GCNGC	4 cut(s)	120, 139, 516, 588
FriOI	GRGCYC	1 cut(s)	468
Fsp4HI	GCNGC	4 cut(s)	120, 139, 516, 588
FspI	TGCGCA	1 cut(s)	437
GlaI	GCGC	5 cut(s)	88, 193, 248, 325, 437
GluI	GCNGC	4 cut(s)	120, 139, 516, 588
GsuI	CTGGAG	1 cut(s)	35
HaeII	RGCGCY	3 cut(s)	90, 195, 250
HaeIII	GGCC	6 cut(s)	113, 122, 141, 362, 411, 559
HapII	CCGG	5 cut(s)	110, 232, 312, 387, 556
HhaI	GCGC	5 cut(s)	89, 194, 249, 326, 438
Hin1I	GRCGYC	1 cut(s)	285
Hin1II	CATG	9 cut(s)	13, 86, 97, 157, 256, 265, 402, 430, 541
Hin6I	GCGC	5 cut(s)	87, 192, 247, 324, 436
HinP1I	GCGC	5 cut(s)	87, 192, 247, 324, 436
HinfI	GANTC	2 cut(s)	71, 218
HpaII	CCGG	5 cut(s)	110, 232, 312, 387, 556
HphI	GGTGA	4 cut(s)	16, 31, 259, 517
Hpy188III	TCNNGA	2 cut(s)	52, 538
Hpy99I	CGWCG	3 cut(s)	290, 299, 353
HpyAV	CCTTC	2 cut(s)	88, 113
HpyCH4IV	ACGT	4 cut(s)	285, 351, 498, 564
HpyCH4V	TGCA	1 cut(s)	398
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 119, 125, 308, 587
HpyF3I	CTNAG	1 cut(s)	335
HpySE526I	ACGT	4 cut(s)	285, 351, 498, 564
Hsp92I	GRCGYC	1 cut(s)	285
Hsp92II	CATG	9 cut(s)	13, 86, 97, 157, 256, 265, 402, 430, 541
HspAI	GCGC	5 cut(s)	87, 192, 247, 324, 436
KroI	GCCGGC	1 cut(s)	109
KroNI	GCCGGC	1 cut(s)	111
Ksp22I	TGATCA	1 cut(s)	264
Kzo9I	GATC	2 cut(s)	264, 600
LguI	GCTCTTC	2 cut(s)	422, 578
LmnI	GCTCC	4 cut(s)	54, 94, 311, 552
Lsp1109I	GCAGC	1 cut(s)	574
MaeII	ACGT	4 cut(s)	285, 351, 498, 564
MalI	GATC	2 cut(s)	266, 602
MboI	GATC	2 cut(s)	264, 600
MboII	GAAGA	3 cut(s)	409, 445, 565
MhlI	GDGCHC	1 cut(s)	468
MlsI	TGGCCA	2 cut(s)	362, 411
MluNI	TGGCCA	2 cut(s)	362, 411
MlyI	GAGTC	1 cut(s)	65
Mox20I	TGGCCA	2 cut(s)	362, 411
MroNI	GCCGGC	1 cut(s)	109
MroXI	GAANNNNTTC	1 cut(s)	572
MscI	TGGCCA	2 cut(s)	362, 411
MseI	TTAA	2 cut(s)	459, 607
MslI	CAYNNNNRTG	2 cut(s)	431, 536
Msp20I	TGGCCA	2 cut(s)	362, 411
MspI	CCGG	5 cut(s)	110, 232, 312, 387, 556
MspR9I	CCNGG	6 cut(s)	232, 233, 244, 313, 387, 556
MvaI	CCWGG	1 cut(s)	244
MwoI	GCNNNNNNNGC	5 cut(s)	11, 119, 125, 308, 587
NaeI	GCCGGC	1 cut(s)	111
NciI	CCSGG	5 cut(s)	232, 233, 313, 387, 556
NcoI	CCATGG	4 cut(s)	9, 82, 93, 426
NdeII	GATC	2 cut(s)	264, 600
NgoMIV	GCCGGC	1 cut(s)	109
NlaIII	CATG	9 cut(s)	13, 86, 97, 157, 256, 265, 402, 430, 541
NlaIV	GGNNCC	2 cut(s)	123, 307
NmeAIII	GCCGAG	1 cut(s)	447
NsbI	TGCGCA	1 cut(s)	437
PagI	TCATGA	1 cut(s)	537
PciSI	GCTCTTC	2 cut(s)	422, 578
PcsI	WCGNNNNNNNCGW	2 cut(s)	291, 375
PdiI	GCCGGC	1 cut(s)	111
PdmI	GAANNNNTTC	1 cut(s)	572
PfeI	GAWTC	1 cut(s)	218
PkrI	GCNGC	4 cut(s)	121, 140, 517, 589
PleI	GAGTC	1 cut(s)	65
PpsI	GAGTC	1 cut(s)	65
Psp124BI	GAGCTC	1 cut(s)	468
Psp6I	CCWGG	1 cut(s)	242
PspGI	CCWGG	1 cut(s)	242
PspN4I	GGNNCC	2 cut(s)	123, 307
PspPI	GGNCC	3 cut(s)	97, 121, 558
RseI	CAYNNNNRTG	2 cut(s)	431, 536
SacI	GAGCTC	1 cut(s)	468
SapI	GCTCTTC	2 cut(s)	422, 578
SaqAI	TTAA	2 cut(s)	459, 607
SatI	GCNGC	4 cut(s)	120, 139, 516, 588
Sau3AI	GATC	2 cut(s)	264, 600
Sau96I	GGNCC	3 cut(s)	97, 121, 558
SchI	GAGTC	1 cut(s)	65
ScrFI	CCNGG	6 cut(s)	232, 233, 244, 313, 387, 556
SduI	GDGCHC	1 cut(s)	468
SfiI	GGCCNNNNNGGCC	1 cut(s)	119
SinI	GGWCC	1 cut(s)	97
SmaI	CCCGGG	1 cut(s)	233
SmiMI	CAYNNNNRTG	2 cut(s)	431, 536
SmlI	CTYRAG	1 cut(s)	187
SmoI	CTYRAG	1 cut(s)	187
SsiI	CCGC	8 cut(s)	42, 100, 119, 126, 138, 309, 444, 516
SstI	GAGCTC	1 cut(s)	468
StyD4I	CCNGG	6 cut(s)	230, 231, 242, 311, 385, 554
StyI	CCWWGG	5 cut(s)	9, 82, 93, 114, 426
TaiI	ACGT	4 cut(s)	288, 354, 501, 567
TaqI	TCGA	4 cut(s)	69, 204, 369, 603
TauI	GCSGC	3 cut(s)	122, 141, 518
TfiI	GAWTC	1 cut(s)	218
Tru1I	TTAA	2 cut(s)	459, 607
Tru9I	TTAA	2 cut(s)	459, 607
TseI	GCWGC	1 cut(s)	587
TspGWI	ACGGA	2 cut(s)	136, 367
TspMI	CCCGGG	1 cut(s)	231
VpaK11BI	GGWCC	1 cut(s)	97
XmaI	CCCGGG	1 cut(s)	231
XmnI	GAANNNNTTC	1 cut(s)	572
ZraI	GACGTC	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.