Rmu_sc0000420.1_g000030

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000420.1
Physical Location & Seq
Forward (+)
147170 .. 149536
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000420.1_g000030.1.cds

Sequence Viewer

Length: 1074 bp
atggcttccacactcgctgcctcctctctctccaccaagctccagaagccatctctcctcgagtcctccttccacggcgttcccgtggctcgggttcagcctttgaaggcggtctcatccaacggccacggcctctccgtgtccatgtccgctggtggctccaccagcgcttacgatttgagcaacttcaagttcgagccgatcaaggagtccatcgtctcccgagagatgaccaggcgatacatgactgacatgatcacctacgccgataccgacgttgtcatcgtcggtgccggttccgcagggctatcttgcgcatgggagctcagcaagaaccccgatgtgcaggtagccattattgagcagtccgtcagccccggcggcggcgcgtggctcggtggccagctcttctccgccatggtagtgaggaaaccagcacatcacttcctcaacgagttgggcattgactacgacgagaaggatgactacgtagtcattaagcatgctgccctattcacctcaactatcatgagcaagctccttgcccgtcccaatgtcaaactcttcaacgccgtagcagctgaggatttgatcatcaagggtggaagagttgctggtgttgtcaccaactgggccttggtctccatgaaccacgacacacagtcgtgcatggaccccaacgttatggagtccaaggttgtggtaagctcatgcggtcacgatgggccaatgggagccactggagtaaagaggttgaggagtgttgggatgattgagagcgtgcctgggatgaaggccttggacatgaatgcagctgaggatgccattgtgaagctaacaagggaaatcgtgcctggaatgatcgttactggcatggaagttgctgagattgatggctctccaagaatgggacctacatttggggctatgatgatatcagggcaaaaggcagcccatttggcattgaaggcactgggacttcccaacgctcttgatggatcatatgttggaggtatccagccagagctgatcttagctgctgcagattcagctgctgagattgctgatgcttga

Protein Analysis

357

Amino Acids

37.42

Weight (kDa)

5.63

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 316
Acc36I ACCTGC 1 cut(s) 337
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 1 cut(s) 389
AciI CCGC 7 cut(s) 110, 150, 300, 381, 384, 414, 714
AclI AACGTT 1 cut(s) 681
AclWI GGATC 1 cut(s) 1006
AcoI YGGCCR 2 cut(s) 124, 400
AfeI AGCGCT 1 cut(s) 169
AfiI CCNNNNNNNGG 4 cut(s) 90, 383, 908, 920
AgsI TTSAA 4 cut(s) 106, 190, 568, 967
AhdI GACNNNNNGTC 1 cut(s) 661
AjnI CCWGG 3 cut(s) 233, 784, 853
AleI CACNNNNGTG 1 cut(s) 664
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 3 cut(s) 118, 223, 646
AlwI GGATC 1 cut(s) 1006
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 3 cut(s) 59, 90, 222
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 6 cut(s) 124, 130, 400, 633, 725, 795
ApeKI GCWGC 8 cut(s) 17, 506, 578, 812, 950, 1037, 1040, 1052
AspLEI GCGC 3 cut(s) 170, 317, 389
AspS9I GGNCC 4 cut(s) 633, 673, 725, 911
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 3 cut(s) 250, 508, 616
AvaI CYCGRG 3 cut(s) 59, 90, 222
AvaII GGWCC 2 cut(s) 673, 911
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 327
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
Bbv12I GWGCWC 1 cut(s) 327
BbvCI CCTCAGC 2 cut(s) 582, 816
BbvI GCAGC 8 cut(s) 4, 493, 590, 824, 962, 1024, 1027, 1039
BccI CCATC 5 cut(s) 58, 221, 716, 887, 989
BceAI ACGGC 4 cut(s) 91, 139, 145, 557
BciT130I CCWGG 3 cut(s) 235, 786, 855
BciVI GTATCC 1 cut(s) 1025
BclI TGATCA 2 cut(s) 255, 591
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 3 cut(s) 118, 223, 646
BfmI CTRYAG 1 cut(s) 1041
BfoI RGCGCY 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 337
BfuI GTATCC 1 cut(s) 1025
BglI GCCNNNNNGGC 2 cut(s) 381, 959
BlpI GCTNAGC 1 cut(s) 326
Bme1390I CCNGG 4 cut(s) 235, 378, 786, 855
Bme18I GGWCC 2 cut(s) 673, 911
BmeRI GACNNNNNGTC 1 cut(s) 661
BmeT110I CYCGRG 3 cut(s) 59, 90, 222
BmgT120I GGNCC 4 cut(s) 633, 673, 725, 911
BmiI GGNNCC 6 cut(s) 160, 292, 298, 675, 736, 912
BmrFI CCNGG 4 cut(s) 235, 378, 786, 855
BmrI ACTGGG 2 cut(s) 640, 983
BmsI GCATC 2 cut(s) 811, 1057
BmuI ACTGGG 2 cut(s) 640, 983
BpmI CTGGAG 2 cut(s) 26, 762
Bpu10I CCTNAGC 2 cut(s) 582, 816
Bpu1102I GCTNAGC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 490
BsaI GGTCTC 2 cut(s) 118, 646
BsaJI CCNNGG 9 cut(s) 73, 84, 127, 376, 417, 636, 693, 785, 798
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 383, 908, 920
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 4 cut(s) 635, 745, 874, 978
BseBI CCWGG 3 cut(s) 235, 786, 855
BseDI CCNNGG 9 cut(s) 73, 84, 127, 376, 417, 636, 693, 785, 798
BseGI GGATG 5 cut(s) 116, 487, 774, 795, 826
BseLI CCNNNNNNNGG 4 cut(s) 90, 383, 908, 920
BseMII CTCAG 5 cut(s) 340, 573, 807, 876, 1047
BseNI ACTGG 4 cut(s) 635, 745, 874, 978
BseRI GAGGAG 3 cut(s) 13, 47, 772
BseXI GCAGC 8 cut(s) 4, 493, 590, 824, 962, 1024, 1027, 1039
BsgI GTGCAG 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 6 cut(s) 126, 132, 402, 635, 727, 797
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 3 cut(s) 59, 90, 222
BsiSI CCGG 2 cut(s) 294, 378
BslFI GGGAC 3 cut(s) 534, 924, 990
BslI CCNNNNNNNGG 4 cut(s) 90, 383, 908, 920
BsmAI GTCTC 3 cut(s) 118, 223, 646
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 3 cut(s) 534, 924, 990
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 6 cut(s) 126, 132, 402, 635, 727, 797
Bso31I GGTCTC 2 cut(s) 118, 646
BsoBI CYCGRG 3 cut(s) 59, 90, 222
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 7 cut(s) 110, 150, 300, 381, 384, 414, 714
BspANI GGCC 6 cut(s) 126, 132, 402, 635, 727, 797
BspCNI CTCAG 5 cut(s) 339, 574, 808, 877, 1048
BspFNI CGCG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 6 cut(s) 160, 292, 298, 675, 736, 912
BspMAI CTGCAG 1 cut(s) 1045
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 1 cut(s) 1006
BspQI GCTCTTC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 2 cut(s) 118, 646
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 4 cut(s) 635, 745, 874, 978
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 9 cut(s) 73, 84, 127, 376, 417, 636, 693, 785, 798
BssMI GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
BssT1I CCWWGG 4 cut(s) 417, 636, 693, 798
Bst2UI CCWGG 3 cut(s) 235, 786, 855
Bst4CI ACNGT 1 cut(s) 663
Bst6I CTCTTC 3 cut(s) 413, 569, 601
BstBAI YACGTR 1 cut(s) 490
BstC8I GCNNGC 4 cut(s) 404, 504, 536, 782
BstDEI CTNAG 6 cut(s) 326, 582, 816, 885, 1033, 1056
BstDSI CCRYGG 4 cut(s) 73, 84, 127, 417
BstF5I GGATG 5 cut(s) 116, 487, 774, 795, 826
BstFNI CGCG 1 cut(s) 389
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 3 cut(s) 170, 317, 389
BstKTI GATC 6 cut(s) 204, 258, 594, 864, 1001, 1032
BstMAI GTCTC 3 cut(s) 118, 223, 646
BstMBI GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
BstNI CCWGG 3 cut(s) 235, 786, 855
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 4 cut(s) 233, 376, 784, 853
BstSFI CTRYAG 1 cut(s) 1041
BstSNI TACGTA 1 cut(s) 490
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 8 cut(s) 4, 493, 590, 824, 962, 1024, 1027, 1039
BstXI CCANNNNNNTGG 2 cut(s) 685, 700
BsuI GTATCC 1 cut(s) 1025
BsuRI GGCC 6 cut(s) 126, 132, 402, 635, 727, 797
BtgI CCRYGG 4 cut(s) 73, 84, 127, 417
BtsCI GGATG 5 cut(s) 116, 487, 774, 795, 826
BtsIMutI CAGTG 2 cut(s) 738, 971
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 4 cut(s) 404, 504, 536, 782
CaiI CAGNNNCTG 1 cut(s) 1055
CciI TCATGA 1 cut(s) 528
CfoI GCGC 3 cut(s) 170, 317, 389
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 4 cut(s) 633, 673, 725, 911
DdeI CTNAG 6 cut(s) 326, 582, 816, 885, 1033, 1056
DpnI GATC 6 cut(s) 203, 257, 593, 863, 1000, 1031
DpnII GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
DrdI GACNNNNNNGTC 1 cut(s) 491
DriI GACNNNNNGTC 1 cut(s) 661
DseDI GACNNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 2 cut(s) 124, 400
Eam1104I CTCTTC 3 cut(s) 413, 569, 601
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 3 cut(s) 413, 569, 601
EciI GGCGGA 1 cut(s) 403
Ecl136II GAGCTC 1 cut(s) 325
Eco105I TACGTA 1 cut(s) 490
Eco130I CCWWGG 4 cut(s) 417, 636, 693, 798
Eco147I AGGCCT 1 cut(s) 797
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 2 cut(s) 118, 646
Eco32I GATATC 1 cut(s) 936
Eco47I GGWCC 2 cut(s) 673, 911
Eco47III AGCGCT 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 325
Eco88I CYCGRG 3 cut(s) 59, 90, 222
EcoICRI GAGCTC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 911
EcoRII CCWGG 3 cut(s) 233, 784, 853
EcoRV GATATC 1 cut(s) 936
EcoT14I CCWWGG 4 cut(s) 417, 636, 693, 798
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 4 cut(s) 417, 636, 693, 798
Esp3I CGTCTC 1 cut(s) 223
FaqI GGGAC 3 cut(s) 534, 924, 990
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 2 cut(s) 255, 591
FokI GGATG 5 cut(s) 103, 494, 781, 802, 833
FriOI GRGCYC 1 cut(s) 327
FspI TGCGCA 1 cut(s) 316
GlaI GCGC 3 cut(s) 169, 316, 388
GsuI CTGGAG 2 cut(s) 26, 762
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 6 cut(s) 126, 132, 402, 635, 727, 797
HapII CCGG 2 cut(s) 294, 378
HhaI GCGC 3 cut(s) 170, 317, 389
Hin6I GCGC 3 cut(s) 168, 315, 387
HinP1I GCGC 3 cut(s) 168, 315, 387
HinfI GANTC 4 cut(s) 62, 209, 689, 1046
HpaII CCGG 2 cut(s) 294, 378
HphI GGTGA 3 cut(s) 250, 508, 616
Hpy188III TCNNGA 5 cut(s) 43, 222, 529, 719, 992
Hpy99I CGWCG 3 cut(s) 278, 290, 476
HpyAV CCTTC 5 cut(s) 79, 100, 472, 787, 961
HpyCH4III ACNGT 1 cut(s) 663
HpyCH4IV ACGT 3 cut(s) 276, 489, 681
HpyCH4V TGCA 4 cut(s) 346, 669, 812, 1043
HpyF3I CTNAG 6 cut(s) 326, 582, 816, 885, 1033, 1056
HpySE526I ACGT 3 cut(s) 276, 489, 681
HspAI GCGC 3 cut(s) 168, 315, 387
Ksp22I TGATCA 2 cut(s) 255, 591
Kzo9I GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 5 cut(s) 45, 164, 322, 543, 734
Lsp1109I GCAGC 8 cut(s) 4, 493, 590, 824, 962, 1024, 1027, 1039
LweI GCATC 2 cut(s) 811, 1057
MaeII ACGT 3 cut(s) 276, 489, 681
MaeIII GTNAC 3 cut(s) 622, 716, 865
MalI GATC 6 cut(s) 203, 257, 593, 863, 1000, 1031
MboI GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
MboII GAAGA 3 cut(s) 400, 556, 618
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 3 cut(s) 71, 218, 698
MmeI TCCRAC 2 cut(s) 144, 988
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 2 cut(s) 422, 664
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 4 cut(s) 152, 581, 815, 1052
MspI CCGG 2 cut(s) 294, 378
MspR9I CCNGG 4 cut(s) 235, 378, 786, 855
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 3 cut(s) 235, 786, 855
MvnI CGCG 1 cut(s) 389
NciI CCSGG 1 cut(s) 378
NcoI CCATGG 1 cut(s) 417
NdeI CATATG 1 cut(s) 1003
NdeII GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
NlaIV GGNNCC 6 cut(s) 160, 292, 298, 675, 736, 912
NmuCI GTSAC 2 cut(s) 622, 716
NsbI TGCGCA 1 cut(s) 316
NspI RCATGY 1 cut(s) 506
OliI CACNNNNGTG 1 cut(s) 664
PaeI GCATGC 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 1 cut(s) 528
PceI AGGCCT 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 135, 270, 282
PctI GAATGC 1 cut(s) 814
PfeI GAWTC 1 cut(s) 1046
PflFI GACNNNGTC 1 cut(s) 278
PleI GAGTC 3 cut(s) 70, 217, 697
PpsI GAGTC 3 cut(s) 70, 217, 697
Ppu21I YACGTR 1 cut(s) 490
PpuMI RGGWCCY 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 911
Psp6I CCWGG 3 cut(s) 233, 784, 853
PspGI CCWGG 3 cut(s) 233, 784, 853
PspN4I GGNNCC 6 cut(s) 160, 292, 298, 675, 736, 912
PspPI GGNCC 4 cut(s) 633, 673, 725, 911
PspPPI RGGWCCY 1 cut(s) 911
PspXI VCTCGAGB 1 cut(s) 59
PstI CTGCAG 1 cut(s) 1045
PstNI CAGNNNCTG 1 cut(s) 1055
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 3 cut(s) 581, 815, 1052
RseI CAYNNNNRTG 2 cut(s) 422, 664
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 498
Sau3AI GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
Sau96I GGNCC 4 cut(s) 633, 673, 725, 911
SchI GAGTC 3 cut(s) 71, 218, 698
ScrFI CCNGG 4 cut(s) 235, 378, 786, 855
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 2 cut(s) 811, 1057
SfcI CTRYAG 1 cut(s) 1041
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 2 cut(s) 673, 911
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 422, 664
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SnaBI TACGTA 1 cut(s) 490
SphI GCATGC 1 cut(s) 506
SseBI AGGCCT 1 cut(s) 797
SsiI CCGC 7 cut(s) 110, 150, 300, 381, 384, 414, 714
SstI GAGCTC 1 cut(s) 327
StuI AGGCCT 1 cut(s) 797
StyD4I CCNGG 4 cut(s) 233, 376, 784, 853
StyI CCWWGG 4 cut(s) 417, 636, 693, 798
TaaI ACNGT 1 cut(s) 663
TaiI ACGT 3 cut(s) 279, 492, 684
TaqI TCGA 2 cut(s) 60, 195
TauI GCSGC 2 cut(s) 384, 387
TfiI GAWTC 1 cut(s) 1046
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 2 cut(s) 745, 978
TseFI GTSAC 2 cut(s) 622, 716
TseI GCWGC 8 cut(s) 17, 506, 578, 812, 950, 1037, 1040, 1052
Tsp45I GTSAC 2 cut(s) 622, 716
TspDTI ATGAA 3 cut(s) 662, 806, 821
TspGWI ACGGA 2 cut(s) 127, 358
TspRI CASTG 2 cut(s) 745, 978
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 673, 911
XceI RCATGY 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XhoI CTCGAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.