Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Reverse (-)

61459247 .. 61463678

Gene Structure 4432 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00043140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGCATCCATGGCTTCCACACTCGCCGCCTCCTCGCTCTCCGCGAAGCTCCAGAAGCCTTCTCTCCTCGACTCCTCCTTCCACGGCGCTCCCGTCGCCCGCGTCCAGCCACTCAAGGCCGCCTCCTCATCCAACGGTCACGGCCTCTCCGTCTCCATGTCCGCCGGCGCCTCCACCAACTCCTATGATCTCAGCAACTTCAAGTTCGAGCCGATCAAGGAGTCCATCGTCTCCAGGGAGATGACCAGGCGCTACATGACTGACATGATCACCTACGCCGACACCGACGTCGTCGTCGTCGGTGCTGGCTCCGCCGGGCTTTCCTGCGCCTACGAGCTCAGCAAGAACCCCGATGTGCAGGTTGCTATTATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCGTGGCTCGGTGGCCAGCTCTTCTCCGCCATGGTGGTGAGGAAACCAGCACATCACTTCCTTAATGAGTTGGGCATTGCCTACGATGAGGCGGATGACTATGTAGTCATCAAGCATGCTGCTCTATTCACATCAACTATCATGAGCAAGCTCCTTGCTCGGCCCAATGTGAAACTCTTCAATGCTGTAGCAGCTGAGGATTTGATCATCAAGGGTGGGAGAGTTGGTGGTGTTGTCACGAACTGGGCCTTGGTCTCCATGAACCATGACACACAGTCGTGCATGGACCCCAACGTTATGGAAGCCAAGGTGGTGGTTAGCTCATGTGGTCACGATGGCCCAATGGGAGCCACTGGCGTGAAGAGGTTGAGGAGCGTTGGGATGATTGACAGCGTGCCTGGGATGAAGGCCTTGGACATGAATGCAGCTGAAGACGCCATTGTGAAGCTAACCAGGGAAATCGTGCCTGGAATGATTGTTACTGGCATGGAAGTTGCTGAGATTGATGGATCTCCAAGAATGGGACCTACATTTGGAGCTATGATGATCTCAGGGCAAAAGGCAGCCCACTTGGCATTGAAGGCGCTAGGACTTCCCAACGCTCTTGATGGAACATATGTTGGAGGCATCCACCCAGAGCTGATCTTAGCTGCTGCAGAATCTGCTTCTGAGATCGCAGATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

361

Amino Acids

37.62

Weight (kDa)

5.72

Isoelectric Point (pI)

33.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	78 - 311	2.9e-113	Thi4 family
FAD_binding_2	PF00890	96 - 132	9.9e-07	FAD binding domain
DAO	PF01266	96 - 143	2.9e-08	FAD dependent oxidoreductase
Pyr_redox_2	PF07992	96 - 137	8e-06	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	99 - 134	6.5e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	287, 293, 503
AatII	GACGTC	1 cut(s)	291
Acc36I	ACCTGC	1 cut(s)	349
AccB1I	GGYRCC	1 cut(s)	167
AccII	CGCG	3 cut(s)	44, 102, 401
AclI	AACGTT	1 cut(s)	693
AclWI	GGATC	1 cut(s)	916
AcoI	YGGCCR	1 cut(s)	412
AcuI	CTGAAG	1 cut(s)	849
AcyI	GRCGYC	3 cut(s)	168, 288, 834
AfiI	CCNNNNNNNGG	3 cut(s)	395, 920, 932
AgsI	TTSAA	3 cut(s)	202, 580, 979
AhdI	GACNNNNNGTC	1 cut(s)	673
AjnI	CCWGG	5 cut(s)	233, 245, 796, 851, 865
AjuI	GAANNNNNNNTTGG	2 cut(s)	632, 664
AleI	CACNNNNGTG	2 cut(s)	676, 755
Alw21I	GWGCWC	1 cut(s)	339
Alw26I	GTCTC	3 cut(s)	157, 235, 658
AlwI	GGATC	1 cut(s)	916
AlwNI	CAGNNNCTG	1 cut(s)	1061
AoxI	GGCC	7 cut(s)	117, 142, 412, 560, 645, 736, 807
ApeKI	GCWGC	6 cut(s)	518, 590, 824, 962, 1049, 1052
Asp700I	GAANNNNTTC	1 cut(s)	575
AspLEI	GCGC	6 cut(s)	89, 170, 252, 329, 401, 985
AspS9I	GGNCC	5 cut(s)	561, 645, 685, 737, 923
AsuC2I	CCSGG	2 cut(s)	316, 390
AsuHPI	GGTGA	2 cut(s)	262, 448
AvaII	GGWCC	2 cut(s)	685, 923
BalI	TGGCCA	1 cut(s)	414
BanI	GGYRCC	1 cut(s)	167
BanII	GRGCYC	1 cut(s)	339
BbsI	GAAGAC	1 cut(s)	837
Bbv12I	GWGCWC	1 cut(s)	339
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	6 cut(s)	505, 602, 836, 974, 1036, 1039
BccI	CCATC	4 cut(s)	233, 728, 899, 1001
BceAI	ACGGC	2 cut(s)	100, 157
BciT130I	CCWGG	5 cut(s)	235, 247, 798, 853, 867
BclI	TGATCA	2 cut(s)	267, 603
BcnI	CCSGG	2 cut(s)	316, 390
BcoDI	GTCTC	3 cut(s)	157, 235, 658
BfaI	CTAG	1 cut(s)	986
BfmI	CTRYAG	2 cut(s)	585, 1053
BfoI	RGCGCY	4 cut(s)	90, 171, 253, 986
BfuAI	ACCTGC	1 cut(s)	349
BglI	GCCNNNNNGGC	2 cut(s)	393, 971
BlpI	GCTNAGC	1 cut(s)	338
Bme1390I	CCNGG	7 cut(s)	235, 247, 316, 390, 798, 853, 867
Bme18I	GGWCC	2 cut(s)	685, 923
BmeRI	GACNNNNNGTC	1 cut(s)	673
BmgT120I	GGNCC	5 cut(s)	561, 645, 685, 737, 923
BmiI	GGNNCC	5 cut(s)	169, 310, 687, 748, 924
BmrFI	CCNGG	7 cut(s)	235, 247, 316, 390, 798, 853, 867
BmrI	ACTGGG	1 cut(s)	652
BmsI	GCATC	3 cut(s)	14, 1035, 1069
BmuI	ACTGGG	1 cut(s)	652
BpiI	GAAGAC	1 cut(s)	837
BpmI	CTGGAG	2 cut(s)	35, 217
Bpu10I	CCTNAGC	1 cut(s)	594
Bpu1102I	GCTNAGC	1 cut(s)	338
BpuEI	CTTGAG	1 cut(s)	98
BpuMI	CCSGG	2 cut(s)	316, 390
BsaHI	GRCGYC	3 cut(s)	168, 288, 834
BsaI	GGTCTC	1 cut(s)	658
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 610, 640
Bsc4I	CCNNNNNNNGG	3 cut(s)	395, 920, 932
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	3 cut(s)	647, 757, 886
Bse3DI	GCAATG	1 cut(s)	474
BseBI	CCWGG	5 cut(s)	235, 247, 798, 853, 867
BseGI	GGATG	6 cut(s)	5, 128, 499, 786, 807, 1026
BseLI	CCNNNNNNNGG	3 cut(s)	395, 920, 932
BseMI	GCAATG	1 cut(s)	474
BseMII	CTCAG	6 cut(s)	205, 352, 585, 888, 963, 1059
BseNI	ACTGG	3 cut(s)	647, 757, 886
BseRI	GAGGAG	5 cut(s)	22, 56, 64, 115, 784
BseXI	GCAGC	6 cut(s)	505, 602, 836, 974, 1036, 1039
BsgI	GTGCAG	1 cut(s)	377
Bsh1236I	CGCG	3 cut(s)	44, 102, 401
BshFI	GGCC	7 cut(s)	119, 144, 414, 562, 647, 738, 809
BshNI	GGYRCC	1 cut(s)	167
BsiHKAI	GWGCWC	1 cut(s)	339
BsiSI	CCGG	3 cut(s)	165, 315, 390
BslFI	GGGAC	1 cut(s)	936
BslI	CCNNNNNNNGG	3 cut(s)	395, 920, 932
BsmAI	GTCTC	3 cut(s)	157, 235, 658
BsmBI	CGTCTC	2 cut(s)	157, 235
BsmFI	GGGAC	1 cut(s)	936
BsmI	GAATGC	1 cut(s)	826
BsnI	GGCC	7 cut(s)	119, 144, 414, 562, 647, 738, 809
Bso31I	GGTCTC	1 cut(s)	658
Bsp1286I	GDGCHC	1 cut(s)	339
Bsp143I	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	2 cut(s)	9, 429
BspANI	GGCC	7 cut(s)	119, 144, 414, 562, 647, 738, 809
BspCNI	CTCAG	6 cut(s)	204, 351, 586, 889, 962, 1060
BspFNI	CGCG	3 cut(s)	44, 102, 401
BspHI	TCATGA	1 cut(s)	540
BspLI	GGNNCC	5 cut(s)	169, 310, 687, 748, 924
BspMAI	CTGCAG	1 cut(s)	1057
BspMI	ACCTGC	1 cut(s)	349
BspPI	GGATC	1 cut(s)	916
BspQI	GCTCTTC	1 cut(s)	425
BspT107I	GGYRCC	1 cut(s)	167
BspTNI	GGTCTC	1 cut(s)	658
BsrDI	GCAATG	1 cut(s)	474
BsrFI	RCCGGY	1 cut(s)	164
BsrI	ACTGG	3 cut(s)	647, 757, 886
BssAI	RCCGGY	1 cut(s)	164
BssMI	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
BssNI	GRCGYC	3 cut(s)	168, 288, 834
BssT1I	CCWWGG	5 cut(s)	9, 429, 648, 705, 810
Bst2UI	CCWGG	5 cut(s)	235, 247, 798, 853, 867
Bst4CI	ACNGT	2 cut(s)	137, 675
Bst6I	CTCTTC	3 cut(s)	425, 581, 755
BstACI	GRCGYC	3 cut(s)	168, 288, 834
BstAPI	GCANNNNNTGC	1 cut(s)	1061
BstC8I	GCNNGC	7 cut(s)	100, 166, 307, 416, 516, 548, 794
BstDEI	CTNAG	7 cut(s)	191, 338, 594, 897, 949, 1045, 1068
BstDSI	CCRYGG	3 cut(s)	9, 82, 429
BstF5I	GGATG	6 cut(s)	5, 128, 499, 786, 807, 1026
BstFNI	CGCG	3 cut(s)	44, 102, 401
BstH2I	RGCGCY	4 cut(s)	90, 171, 253, 986
BstHHI	GCGC	6 cut(s)	89, 170, 252, 329, 401, 985
BstKTI	GATC	8 cut(s)	190, 216, 270, 606, 911, 948, 1044, 1074
BstMAI	GTCTC	3 cut(s)	157, 235, 658
BstMBI	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
BstNI	CCWGG	5 cut(s)	235, 247, 798, 853, 867
BstNSI	RCATGY	1 cut(s)	518
BstSCI	CCNGG	7 cut(s)	233, 245, 314, 388, 796, 851, 865
BstSFI	CTRYAG	2 cut(s)	585, 1053
BstUI	CGCG	3 cut(s)	44, 102, 401
BstV1I	GCAGC	6 cut(s)	505, 602, 836, 974, 1036, 1039
BstV2I	GAAGAC	1 cut(s)	837
BstX2I	RGATCY	1 cut(s)	908
BstXI	CCANNNNNNTGG	2 cut(s)	697, 712
BstYI	RGATCY	1 cut(s)	908
BsuRI	GGCC	7 cut(s)	119, 144, 414, 562, 647, 738, 809
BtgI	CCRYGG	3 cut(s)	9, 82, 429
BtsCI	GGATG	6 cut(s)	5, 128, 499, 786, 807, 1026
BtsIMutI	CAGTG	1 cut(s)	750
BveI	ACCTGC	1 cut(s)	349
Cac8I	GCNNGC	7 cut(s)	100, 166, 307, 416, 516, 548, 794
CaiI	CAGNNNCTG	1 cut(s)	1061
CciI	TCATGA	1 cut(s)	540
CfoI	GCGC	6 cut(s)	89, 170, 252, 329, 401, 985
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	5 cut(s)	561, 645, 685, 737, 923
CseI	GACGC	2 cut(s)	91, 842
DdeI	CTNAG	7 cut(s)	191, 338, 594, 897, 949, 1045, 1068
DinI	GGCGCC	1 cut(s)	169
DpnI	GATC	8 cut(s)	189, 215, 269, 605, 910, 947, 1043, 1073
DpnII	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
DrdI	GACNNNNNNGTC	3 cut(s)	287, 293, 503
DriI	GACNNNNNGTC	1 cut(s)	673
DseDI	GACNNNNNNGTC	3 cut(s)	287, 293, 503
EaeI	YGGCCR	1 cut(s)	412
Eam1104I	CTCTTC	3 cut(s)	425, 581, 755
Eam1105I	GACNNNNNGTC	1 cut(s)	673
EarI	CTCTTC	3 cut(s)	425, 581, 755
EciI	GGCGGA	4 cut(s)	151, 301, 415, 506
Ecl136II	GAGCTC	1 cut(s)	337
Eco130I	CCWWGG	5 cut(s)	9, 429, 648, 705, 810
Eco147I	AGGCCT	1 cut(s)	809
Eco24I	GRGCYC	1 cut(s)	339
Eco31I	GGTCTC	1 cut(s)	658
Eco47I	GGWCC	2 cut(s)	685, 923
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	1 cut(s)	849
EcoICRI	GAGCTC	1 cut(s)	337
EcoO109I	RGGNCCY	1 cut(s)	923
EcoRII	CCWGG	5 cut(s)	233, 245, 796, 851, 865
EcoT14I	CCWWGG	5 cut(s)	9, 429, 648, 705, 810
EcoT38I	GRGCYC	1 cut(s)	339
EgeI	GGCGCC	1 cut(s)	169
EheI	GGCGCC	1 cut(s)	169
ErhI	CCWWGG	5 cut(s)	9, 429, 648, 705, 810
Esp3I	CGTCTC	2 cut(s)	157, 235
FaqI	GGGAC	1 cut(s)	936
FauI	CCCGC	1 cut(s)	107
FauNDI	CATATG	1 cut(s)	1015
FbaI	TGATCA	2 cut(s)	267, 603
FokI	GGATG	5 cut(s)	115, 506, 793, 814, 1013
FriOI	GRGCYC	1 cut(s)	339
FspBI	CTAG	1 cut(s)	986
GlaI	GCGC	6 cut(s)	88, 169, 251, 328, 400, 984
GsuI	CTGGAG	2 cut(s)	35, 217
HaeII	RGCGCY	4 cut(s)	90, 171, 253, 986
HaeIII	GGCC	7 cut(s)	119, 144, 414, 562, 647, 738, 809
HapII	CCGG	3 cut(s)	165, 315, 390
HgaI	GACGC	2 cut(s)	91, 842
HhaI	GCGC	6 cut(s)	89, 170, 252, 329, 401, 985
Hin1I	GRCGYC	3 cut(s)	168, 288, 834
Hin6I	GCGC	6 cut(s)	87, 168, 250, 327, 399, 983
HinP1I	GCGC	6 cut(s)	87, 168, 250, 327, 399, 983
HinfI	GANTC	3 cut(s)	71, 221, 1058
HpaII	CCGG	3 cut(s)	165, 315, 390
HphI	GGTGA	2 cut(s)	262, 448
Hpy188I	TCNGA	1 cut(s)	1069
Hpy188III	TCNNGA	5 cut(s)	52, 541, 637, 731, 1004
Hpy99I	CGWCG	6 cut(s)	98, 290, 293, 296, 299, 302
HpyAV	CCTTC	4 cut(s)	69, 88, 799, 973
HpyCH4III	ACNGT	2 cut(s)	137, 675
HpyCH4IV	ACGT	2 cut(s)	288, 693
HpyCH4V	TGCA	4 cut(s)	358, 681, 824, 1055
HpyF3I	CTNAG	7 cut(s)	191, 338, 594, 897, 949, 1045, 1068
HpySE526I	ACGT	2 cut(s)	288, 693
Hsp92I	GRCGYC	3 cut(s)	168, 288, 834
HspAI	GCGC	6 cut(s)	87, 168, 250, 327, 399, 983
KasI	GGCGCC	1 cut(s)	167
KroI	GCCGGC	1 cut(s)	164
KroNI	GCCGGC	1 cut(s)	166
Ksp22I	TGATCA	2 cut(s)	267, 603
Kzo9I	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
LguI	GCTCTTC	1 cut(s)	425
LmnI	GCTCC	7 cut(s)	54, 94, 314, 555, 746, 771, 935
Lsp1109I	GCAGC	6 cut(s)	505, 602, 836, 974, 1036, 1039
LweI	GCATC	3 cut(s)	14, 1035, 1069
MaeI	CTAG	1 cut(s)	986
MaeII	ACGT	2 cut(s)	288, 693
MaeIII	GTNAC	4 cut(s)	137, 634, 728, 877
MalI	GATC	8 cut(s)	189, 215, 269, 605, 910, 947, 1043, 1073
MboI	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
MboII	GAAGA	4 cut(s)	412, 568, 772, 842
MflI	RGATCY	1 cut(s)	908
MhlI	GDGCHC	1 cut(s)	339
MlsI	TGGCCA	1 cut(s)	414
MluNI	TGGCCA	1 cut(s)	414
Mly113I	GGCGCC	1 cut(s)	168
MlyI	GAGTC	2 cut(s)	65, 230
MmeI	TCCRAC	2 cut(s)	156, 1000
Mox20I	TGGCCA	1 cut(s)	414
MreI	CGCCGGCG	1 cut(s)	164
MroNI	GCCGGC	1 cut(s)	164
MroXI	GAANNNNTTC	1 cut(s)	575
MscI	TGGCCA	1 cut(s)	414
MseI	TTAA	2 cut(s)	462, 1084
MslI	CAYNNNNRTG	3 cut(s)	434, 676, 755
Msp20I	TGGCCA	1 cut(s)	414
MspA1I	CMGCKG	2 cut(s)	593, 827
MspI	CCGG	3 cut(s)	165, 315, 390
MspR9I	CCNGG	7 cut(s)	235, 247, 316, 390, 798, 853, 867
Mva1269I	GAATGC	1 cut(s)	826
MvaI	CCWGG	5 cut(s)	235, 247, 798, 853, 867
MvnI	CGCG	3 cut(s)	44, 102, 401
NaeI	GCCGGC	1 cut(s)	166
NarI	GGCGCC	1 cut(s)	168
NciI	CCSGG	2 cut(s)	316, 390
NcoI	CCATGG	2 cut(s)	9, 429
NdeI	CATATG	1 cut(s)	1015
NdeII	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
NgoMIV	GCCGGC	1 cut(s)	164
NlaIV	GGNNCC	5 cut(s)	169, 310, 687, 748, 924
NmeAIII	GCCGAG	1 cut(s)	538
NmuCI	GTSAC	3 cut(s)	137, 634, 728
NspI	RCATGY	1 cut(s)	518
OliI	CACNNNNGTG	2 cut(s)	676, 755
PaeI	GCATGC	1 cut(s)	518
PagI	TCATGA	1 cut(s)	540
PceI	AGGCCT	1 cut(s)	809
PciSI	GCTCTTC	1 cut(s)	425
PcsI	WCGNNNNNNNCGW	5 cut(s)	41, 90, 147, 282, 294
PctI	GAATGC	1 cut(s)	826
PdiI	GCCGGC	1 cut(s)	166
PdmI	GAANNNNTTC	1 cut(s)	575
PfeI	GAWTC	1 cut(s)	1058
PflFI	GACNNNGTC	1 cut(s)	290
PleI	GAGTC	2 cut(s)	65, 229
PluTI	GGCGCC	1 cut(s)	171
PpsI	GAGTC	2 cut(s)	65, 229
PpuMI	RGGWCCY	1 cut(s)	923
Psp124BI	GAGCTC	1 cut(s)	339
Psp1406I	AACGTT	1 cut(s)	693
Psp5II	RGGWCCY	1 cut(s)	923
Psp6I	CCWGG	5 cut(s)	233, 245, 796, 851, 865
PspGI	CCWGG	5 cut(s)	233, 245, 796, 851, 865
PspN4I	GGNNCC	5 cut(s)	169, 310, 687, 748, 924
PspPI	GGNCC	5 cut(s)	561, 645, 685, 737, 923
PspPPI	RGGWCCY	1 cut(s)	923
PstI	CTGCAG	1 cut(s)	1057
PstNI	CAGNNNCTG	1 cut(s)	1061
PsuI	RGATCY	1 cut(s)	908
PsyI	GACNNNGTC	1 cut(s)	290
PvuII	CAGCTG	2 cut(s)	593, 827
RseI	CAYNNNNRTG	3 cut(s)	434, 676, 755
SacI	GAGCTC	1 cut(s)	339
SapI	GCTCTTC	1 cut(s)	425
SaqAI	TTAA	2 cut(s)	462, 1084
Sau3AI	GATC	8 cut(s)	187, 213, 267, 603, 908, 945, 1041, 1071
Sau96I	GGNCC	5 cut(s)	561, 645, 685, 737, 923
SchI	GAGTC	2 cut(s)	65, 230
ScrFI	CCNGG	7 cut(s)	235, 247, 316, 390, 798, 853, 867
SduI	GDGCHC	1 cut(s)	339
SfaNI	GCATC	3 cut(s)	14, 1035, 1069
SfcI	CTRYAG	2 cut(s)	585, 1053
SfoI	GGCGCC	1 cut(s)	169
SgrAI	CRCCGGYG	1 cut(s)	164
SinI	GGWCC	2 cut(s)	685, 923
SmiMI	CAYNNNNRTG	3 cut(s)	434, 676, 755
SmlI	CTYRAG	1 cut(s)	113
SmoI	CTYRAG	1 cut(s)	113
SphI	GCATGC	1 cut(s)	518
SseBI	AGGCCT	1 cut(s)	809
SspDI	GGCGCC	1 cut(s)	167
SspMI	CTAG	1 cut(s)	986
SstI	GAGCTC	1 cut(s)	339
StuI	AGGCCT	1 cut(s)	809
StyD4I	CCNGG	7 cut(s)	233, 245, 314, 388, 796, 851, 865
StyI	CCWWGG	5 cut(s)	9, 429, 648, 705, 810
TaaI	ACNGT	2 cut(s)	137, 675
TaiI	ACGT	2 cut(s)	291, 696
TaqI	TCGA	2 cut(s)	69, 207
TauI	GCSGC	4 cut(s)	29, 122, 396, 399
TfiI	GAWTC	1 cut(s)	1058
Tru1I	TTAA	2 cut(s)	462, 1084
Tru9I	TTAA	2 cut(s)	462, 1084
TscAI	CASTG	1 cut(s)	757
TseFI	GTSAC	3 cut(s)	137, 634, 728
TseI	GCWGC	6 cut(s)	518, 590, 824, 962, 1049, 1052
Tsp45I	GTSAC	3 cut(s)	137, 634, 728
TspDTI	ATGAA	3 cut(s)	674, 818, 833
TspGWI	ACGGA	2 cut(s)	139, 370
TspRI	CASTG	1 cut(s)	757
Tth111I	GACNNNGTC	1 cut(s)	290
VpaK11BI	GGWCC	2 cut(s)	685, 923
XceI	RCATGY	1 cut(s)	518
XmnI	GAANNNNTTC	1 cut(s)	575
XspI	CTAG	1 cut(s)	986
ZraI	GACGTC	1 cut(s)	289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.