Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Reverse (-)

66085502 .. 66088349

Gene Structure 2848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ33729

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGCGAATCTTTGGAAATTATTGCAACCACCGGGGGCTCTATTGAAACCTGAAAACCTCAGGGGCCTGCACATCACTCCAGATATTTCACACCTCGGTGTCCATAAATACACTCTCCCAGTTACACACTCACCACTCTTAAGTCCCATCTTCAACAAAACCAAACCCCCATATCTGTTCCCTCTCTCTCTTCATACCATCAAAATGGCATCCATGGCTTCCACACTCGCTGCCTTCTCACTCTCCACCAAGCTCCAGAAGCCATCTCTCCTCGAATCCTCCTTCCACGGCGTTCCCGTGGCTCGGGTTCAGCCTTTGAAGGCGGTCTCATCCAACGGCCACGGCCTCTCCGTGTCCATGTCCGATGGTGGCTCCACCAGCGCTTACGATTTGAGCAACTTCAAGTTCGAGCCGATCAAGGAGTCCATCGTCTCCCGAGAGATGACCAGGCGATACATGACTGACATGATCACCTACGCCGATACCGACGTTGTCATCGTCGGTGCCGGTGCCGCAGGGCTATCTTGCGCATGGGAGCTCAGCAAGAACCCCGATGTGCAGGTAGCCATTATTGAGCAGTCCGTCGGCCCTGGCGGCGGCGCGTGGCTCGGTGGCCAGCTCTTCTCCGCCATGGTAGTGAGGAAACCAGCACATCACTTCCTCAACGAGTTGGGCATTGACTACGACGAGAAGGATGACTACGTAGTCATTAAGCATGCTGCCCTATTCACCTCAACTATCATGAGCAAGCTCCTTGCCCGTCCCAATGTCAAACTCTTCAACGCCGTAGCATCTGAGGATTTGATCATCAAGGGTGGAAGAGTTGCTGGTGTCGTCACCAACTGGGCCTTGGTCTCCATGAACCACGACACACAGTCGTGCATGGACCCCAACGTTATGGAGTCCAAGGTTGTGGTAAGCTCATGCGGTCACGATGGGCCAATGGGGGCCACTGGAGTAAAGAGGTTGAGGAGTGTTGGGATGATTGAGAGCGTGCCTGGGATGACGGCCTTGGACATGAATGCAGCTGAGGATGCCATTGTGAAGCTAACAAGGGAAATCGTGCCTGGAATGATCGTTACTGGCATGGAAGTTGCTGAGATTGATGGCTCTCCAAGAATGGGACCAACATTCGGGGCTATGATGATATCAGGGCAAAAGGCAGCCCATTTGGCATTGAAGGCACTGGGACTTCCCAACGCTCTTGATGGATCATATGTTGGAGGTATCCAGCCAGAGCTGATCTTAGCTGCTGCAGATTCTGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

423

Amino Acids

44.89

Weight (kDa)

6.67

Isoelectric Point (pI)

38.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	145 - 378	1.7e-110	Thi4 family
DAO	PF01266	163 - 211	2.6e-08	FAD dependent oxidoreductase
FAD_binding_2	PF00890	163 - 197	3.3e-06	FAD binding domain
NAD_binding_8	PF13450	166 - 201	6.2e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	704
Acc16I	TGCGCA	1 cut(s)	529
Acc36I	ACCTGC	1 cut(s)	550
AccB1I	GGYRCC	2 cut(s)	503, 509
AccII	CGCG	1 cut(s)	602
AciI	CCGC	6 cut(s)	323, 513, 594, 597, 627, 927
AclI	AACGTT	1 cut(s)	894
AclWI	GGATC	1 cut(s)	1219
AcoI	YGGCCR	2 cut(s)	337, 613
AfeI	AGCGCT	1 cut(s)	382
AfiI	CCNNNNNNNGG	4 cut(s)	303, 596, 1121, 1133
AflII	CTTAAG	1 cut(s)	139
AgsI	TTSAA	6 cut(s)	46, 154, 319, 403, 781, 1180
AhdI	GACNNNNNGTC	1 cut(s)	874
AjnI	CCWGG	4 cut(s)	446, 589, 997, 1066
AleI	CACNNNNGTG	2 cut(s)	96, 877
AluBI	AGCT	9 cut(s)	253, 538, 619, 751, 921, 1028, 1048, 1240, 1250
AluI	AGCT	9 cut(s)	253, 538, 619, 751, 921, 1028, 1048, 1240, 1250
Alw21I	GWGCWC	1 cut(s)	540
Alw26I	GTCTC	3 cut(s)	331, 436, 859
AlwI	GGATC	1 cut(s)	1219
AlwNI	CAGNNNCTG	1 cut(s)	1262
Ama87I	CYCGRG	2 cut(s)	303, 435
Aor51HI	AGCGCT	1 cut(s)	382
AoxI	GGCC	9 cut(s)	64, 337, 343, 586, 613, 846, 938, 948, 1008
ApeKI	GCWGC	7 cut(s)	230, 719, 1025, 1163, 1250, 1253, 1265
AspLEI	GCGC	3 cut(s)	383, 530, 602
AspS9I	GGNCC	7 cut(s)	64, 587, 846, 886, 938, 948, 1124
AsuC2I	CCSGG	1 cut(s)	33
AsuHPI	GGTGA	4 cut(s)	123, 463, 721, 829
AvaI	CYCGRG	2 cut(s)	303, 435
AvaII	GGWCC	2 cut(s)	886, 1124
AxyI	CCTNAGG	1 cut(s)	59
BaeI	ACNNNNGTAYC	2 cut(s)	474, 507
BalI	TGGCCA	1 cut(s)	615
BanI	GGYRCC	2 cut(s)	503, 509
BanII	GRGCYC	2 cut(s)	40, 540
BarI	GAAGNNNNNNTAC	2 cut(s)	683, 715
Bbv12I	GWGCWC	1 cut(s)	540
BbvCI	CCTCAGC	1 cut(s)	1029
BbvI	GCAGC	7 cut(s)	217, 706, 1037, 1175, 1237, 1240, 1252
BccI	CCATC	8 cut(s)	155, 206, 271, 359, 434, 929, 1100, 1202
BceAI	ACGGC	5 cut(s)	304, 352, 358, 770, 1023
BciT130I	CCWGG	4 cut(s)	448, 591, 999, 1068
BciVI	GTATCC	1 cut(s)	1238
BclI	TGATCA	2 cut(s)	468, 804
BcnI	CCSGG	1 cut(s)	33
BcoDI	GTCTC	3 cut(s)	331, 436, 859
BfmI	CTRYAG	1 cut(s)	1254
BfoI	RGCGCY	1 cut(s)	384
BfrI	CTTAAG	1 cut(s)	139
BfuAI	ACCTGC	1 cut(s)	550
BfuI	GTATCC	1 cut(s)	1238
BglI	GCCNNNNNGGC	2 cut(s)	594, 1172
BlpI	GCTNAGC	1 cut(s)	539
Bme1390I	CCNGG	5 cut(s)	33, 448, 591, 999, 1068
Bme18I	GGWCC	2 cut(s)	886, 1124
BmeRI	GACNNNNNGTC	1 cut(s)	874
BmeT110I	CYCGRG	2 cut(s)	303, 435
BmgT120I	GGNCC	7 cut(s)	64, 587, 846, 886, 938, 948, 1124
BmiI	GGNNCC	7 cut(s)	65, 373, 505, 511, 888, 949, 1125
BmrFI	CCNGG	5 cut(s)	33, 448, 591, 999, 1068
BmrI	ACTGGG	3 cut(s)	113, 853, 1196
BmsI	GCATC	3 cut(s)	218, 800, 1024
BmuI	ACTGGG	3 cut(s)	113, 853, 1196
BpmI	CTGGAG	3 cut(s)	63, 239, 975
Bpu10I	CCTNAGC	1 cut(s)	1029
Bpu1102I	GCTNAGC	1 cut(s)	539
BpuMI	CCSGG	1 cut(s)	33
BsaAI	YACGTR	1 cut(s)	703
BsaI	GGTCTC	2 cut(s)	331, 859
BsaXI	ACNNNNNCTCC	2 cut(s)	332, 362
Bsc4I	CCNNNNNNNGG	4 cut(s)	303, 596, 1121, 1133
Bse118I	RCCGGY	1 cut(s)	506
Bse1I	ACTGG	5 cut(s)	119, 848, 958, 1087, 1191
Bse21I	CCTNAGG	1 cut(s)	59
BseBI	CCWGG	4 cut(s)	448, 591, 999, 1068
BseGI	GGATG	6 cut(s)	209, 329, 700, 987, 1008, 1039
BseLI	CCNNNNNNNGG	4 cut(s)	303, 596, 1121, 1133
BseMII	CTCAG	5 cut(s)	73, 553, 786, 1020, 1089
BseNI	ACTGG	5 cut(s)	119, 848, 958, 1087, 1191
BseRI	GAGGAG	2 cut(s)	260, 985
BseXI	GCAGC	7 cut(s)	217, 706, 1037, 1175, 1237, 1240, 1252
BsgI	GTGCAG	2 cut(s)	53, 578
Bsh1236I	CGCG	1 cut(s)	602
BshFI	GGCC	9 cut(s)	66, 339, 345, 588, 615, 848, 940, 950, 1010
BshNI	GGYRCC	2 cut(s)	503, 509
BsiHKAI	GWGCWC	1 cut(s)	540
BsiHKCI	CYCGRG	2 cut(s)	303, 435
BsiSI	CCGG	2 cut(s)	32, 507
BslFI	GGGAC	4 cut(s)	129, 747, 1137, 1203
BslI	CCNNNNNNNGG	4 cut(s)	303, 596, 1121, 1133
BsmAI	GTCTC	3 cut(s)	331, 436, 859
BsmBI	CGTCTC	1 cut(s)	436
BsmFI	GGGAC	4 cut(s)	129, 747, 1137, 1203
BsmI	GAATGC	1 cut(s)	1027
BsnI	GGCC	9 cut(s)	66, 339, 345, 588, 615, 848, 940, 950, 1010
Bso31I	GGTCTC	2 cut(s)	331, 859
BsoBI	CYCGRG	2 cut(s)	303, 435
Bsp1286I	GDGCHC	2 cut(s)	40, 540
Bsp143I	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
Bsp1720I	GCTNAGC	1 cut(s)	539
Bsp19I	CCATGG	2 cut(s)	213, 630
BspACI	CCGC	6 cut(s)	323, 513, 594, 597, 627, 927
BspANI	GGCC	9 cut(s)	66, 339, 345, 588, 615, 848, 940, 950, 1010
BspCNI	CTCAG	5 cut(s)	72, 552, 787, 1021, 1090
BspFNI	CGCG	1 cut(s)	602
BspHI	TCATGA	1 cut(s)	741
BspLI	GGNNCC	7 cut(s)	65, 373, 505, 511, 888, 949, 1125
BspMAI	CTGCAG	1 cut(s)	1258
BspMI	ACCTGC	1 cut(s)	550
BspPI	GGATC	1 cut(s)	1219
BspQI	GCTCTTC	1 cut(s)	626
BspT107I	GGYRCC	2 cut(s)	503, 509
BspTI	CTTAAG	1 cut(s)	139
BspTNI	GGTCTC	2 cut(s)	331, 859
BsrFI	RCCGGY	1 cut(s)	506
BsrI	ACTGG	5 cut(s)	119, 848, 958, 1087, 1191
BssAI	RCCGGY	1 cut(s)	506
BssMI	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
BssT1I	CCWWGG	5 cut(s)	213, 630, 849, 906, 1011
Bst2UI	CCWGG	4 cut(s)	448, 591, 999, 1068
Bst4CI	ACNGT	1 cut(s)	876
Bst6I	CTCTTC	4 cut(s)	195, 626, 782, 814
BstAFI	CTTAAG	1 cut(s)	139
BstAPI	GCANNNNNTGC	1 cut(s)	1262
BstBAI	YACGTR	1 cut(s)	703
BstC8I	GCNNGC	5 cut(s)	68, 617, 717, 749, 995
BstDEI	CTNAG	6 cut(s)	59, 539, 795, 1029, 1098, 1246
BstDSI	CCRYGG	5 cut(s)	213, 286, 297, 340, 630
BstF5I	GGATG	6 cut(s)	209, 329, 700, 987, 1008, 1039
BstFNI	CGCG	1 cut(s)	602
BstH2I	RGCGCY	1 cut(s)	384
BstHHI	GCGC	3 cut(s)	383, 530, 602
BstKTI	GATC	6 cut(s)	417, 471, 807, 1077, 1214, 1245
BstMAI	GTCTC	3 cut(s)	331, 436, 859
BstMBI	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
BstMWI	GCNNNNNNNGC	9 cut(s)	215, 259, 378, 512, 594, 1034, 1172, 1181, 1262
BstNI	CCWGG	4 cut(s)	448, 591, 999, 1068
BstNSI	RCATGY	1 cut(s)	719
BstSCI	CCNGG	5 cut(s)	31, 446, 589, 997, 1066
BstSFI	CTRYAG	1 cut(s)	1254
BstSNI	TACGTA	1 cut(s)	703
BstUI	CGCG	1 cut(s)	602
BstV1I	GCAGC	7 cut(s)	217, 706, 1037, 1175, 1237, 1240, 1252
BstXI	CCANNNNNNTGG	3 cut(s)	205, 898, 913
Bsu36I	CCTNAGG	1 cut(s)	59
BsuI	GTATCC	1 cut(s)	1238
BsuRI	GGCC	9 cut(s)	66, 339, 345, 588, 615, 848, 940, 950, 1010
BtgI	CCRYGG	5 cut(s)	213, 286, 297, 340, 630
BtsCI	GGATG	6 cut(s)	209, 329, 700, 987, 1008, 1039
BtsIMutI	CAGTG	2 cut(s)	951, 1184
BveI	ACCTGC	1 cut(s)	550
Cac8I	GCNNGC	5 cut(s)	68, 617, 717, 749, 995
CaiI	CAGNNNCTG	1 cut(s)	1262
CciI	TCATGA	1 cut(s)	741
CfoI	GCGC	3 cut(s)	383, 530, 602
Cfr10I	RCCGGY	1 cut(s)	506
Cfr13I	GGNCC	7 cut(s)	64, 587, 846, 886, 938, 948, 1124
DdeI	CTNAG	6 cut(s)	59, 539, 795, 1029, 1098, 1246
DpnI	GATC	6 cut(s)	416, 470, 806, 1076, 1213, 1244
DpnII	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
DrdI	GACNNNNNNGTC	1 cut(s)	704
DriI	GACNNNNNGTC	1 cut(s)	874
DseDI	GACNNNNNNGTC	1 cut(s)	704
EaeI	YGGCCR	2 cut(s)	337, 613
Eam1104I	CTCTTC	4 cut(s)	195, 626, 782, 814
Eam1105I	GACNNNNNGTC	1 cut(s)	874
EarI	CTCTTC	4 cut(s)	195, 626, 782, 814
EciI	GGCGGA	1 cut(s)	616
Ecl136II	GAGCTC	1 cut(s)	538
Eco105I	TACGTA	1 cut(s)	703
Eco130I	CCWWGG	5 cut(s)	213, 630, 849, 906, 1011
Eco24I	GRGCYC	2 cut(s)	40, 540
Eco31I	GGTCTC	2 cut(s)	331, 859
Eco32I	GATATC	1 cut(s)	1149
Eco47I	GGWCC	2 cut(s)	886, 1124
Eco47III	AGCGCT	1 cut(s)	382
Eco53kI	GAGCTC	1 cut(s)	538
Eco81I	CCTNAGG	1 cut(s)	59
Eco88I	CYCGRG	2 cut(s)	303, 435
EcoICRI	GAGCTC	1 cut(s)	538
EcoO109I	RGGNCCY	1 cut(s)	64
EcoRII	CCWGG	4 cut(s)	446, 589, 997, 1066
EcoRV	GATATC	1 cut(s)	1149
EcoT14I	CCWWGG	5 cut(s)	213, 630, 849, 906, 1011
EcoT38I	GRGCYC	2 cut(s)	40, 540
ErhI	CCWWGG	5 cut(s)	213, 630, 849, 906, 1011
Esp3I	CGTCTC	1 cut(s)	436
FaqI	GGGAC	4 cut(s)	129, 747, 1137, 1203
FauNDI	CATATG	1 cut(s)	1216
FbaI	TGATCA	2 cut(s)	468, 804
FokI	GGATG	6 cut(s)	196, 316, 707, 994, 1015, 1046
FriOI	GRGCYC	2 cut(s)	40, 540
FspI	TGCGCA	1 cut(s)	529
GlaI	GCGC	3 cut(s)	382, 529, 601
GsuI	CTGGAG	3 cut(s)	63, 239, 975
HaeII	RGCGCY	1 cut(s)	384
HaeIII	GGCC	9 cut(s)	66, 339, 345, 588, 615, 848, 940, 950, 1010
HapII	CCGG	2 cut(s)	32, 507
HhaI	GCGC	3 cut(s)	383, 530, 602
Hin6I	GCGC	3 cut(s)	381, 528, 600
HinP1I	GCGC	3 cut(s)	381, 528, 600
HinfI	GANTC	5 cut(s)	7, 275, 422, 902, 1259
HpaII	CCGG	2 cut(s)	32, 507
HphI	GGTGA	4 cut(s)	123, 463, 721, 829
Hpy188I	TCNGA	2 cut(s)	364, 796
Hpy188III	TCNNGA	6 cut(s)	80, 256, 435, 742, 932, 1205
Hpy99I	CGWCG	4 cut(s)	491, 503, 587, 689
HpyAV	CCTTC	5 cut(s)	244, 292, 313, 685, 1174
HpyCH4III	ACNGT	1 cut(s)	876
HpyCH4IV	ACGT	3 cut(s)	489, 702, 894
HpyCH4V	TGCA	6 cut(s)	25, 70, 559, 882, 1025, 1256
HpyF10VI	GCNNNNNNNGC	9 cut(s)	215, 259, 378, 512, 594, 1034, 1172, 1181, 1262
HpyF3I	CTNAG	6 cut(s)	59, 539, 795, 1029, 1098, 1246
HpySE526I	ACGT	3 cut(s)	489, 702, 894
HspAI	GCGC	3 cut(s)	381, 528, 600
Ksp22I	TGATCA	2 cut(s)	468, 804
Kzo9I	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
LguI	GCTCTTC	1 cut(s)	626
LmnI	GCTCC	4 cut(s)	258, 377, 535, 756
Lsp1109I	GCAGC	7 cut(s)	217, 706, 1037, 1175, 1237, 1240, 1252
LweI	GCATC	3 cut(s)	218, 800, 1024
MaeII	ACGT	3 cut(s)	489, 702, 894
MaeIII	GTNAC	4 cut(s)	121, 835, 929, 1078
MalI	GATC	6 cut(s)	416, 470, 806, 1076, 1213, 1244
MboI	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
MboII	GAAGA	5 cut(s)	142, 182, 613, 769, 831
MhlI	GDGCHC	2 cut(s)	40, 540
MlsI	TGGCCA	1 cut(s)	615
MluCI	AATT	1 cut(s)	17
MluNI	TGGCCA	1 cut(s)	615
MlyI	GAGTC	2 cut(s)	431, 911
MmeI	TCCRAC	2 cut(s)	357, 1201
Mox20I	TGGCCA	1 cut(s)	615
MscI	TGGCCA	1 cut(s)	615
MseI	TTAA	2 cut(s)	140, 711
MslI	CAYNNNNRTG	4 cut(s)	96, 203, 635, 877
Msp20I	TGGCCA	1 cut(s)	615
MspA1I	CMGCKG	1 cut(s)	1028
MspCI	CTTAAG	1 cut(s)	139
MspI	CCGG	2 cut(s)	32, 507
MspR9I	CCNGG	5 cut(s)	33, 448, 591, 999, 1068
Mva1269I	GAATGC	1 cut(s)	1027
MvaI	CCWGG	4 cut(s)	448, 591, 999, 1068
MvnI	CGCG	1 cut(s)	602
MwoI	GCNNNNNNNGC	9 cut(s)	215, 259, 378, 512, 594, 1034, 1172, 1181, 1262
NciI	CCSGG	1 cut(s)	33
NcoI	CCATGG	2 cut(s)	213, 630
NdeI	CATATG	1 cut(s)	1216
NdeII	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
NlaIV	GGNNCC	7 cut(s)	65, 373, 505, 511, 888, 949, 1125
NmuCI	GTSAC	2 cut(s)	835, 929
NsbI	TGCGCA	1 cut(s)	529
NspI	RCATGY	1 cut(s)	719
OliI	CACNNNNGTG	2 cut(s)	96, 877
PaeI	GCATGC	1 cut(s)	719
PagI	TCATGA	1 cut(s)	741
PciSI	GCTCTTC	1 cut(s)	626
PcsI	WCGNNNNNNNCGW	4 cut(s)	294, 348, 483, 495
PctI	GAATGC	1 cut(s)	1027
PfeI	GAWTC	3 cut(s)	7, 275, 1259
PflFI	GACNNNGTC	1 cut(s)	491
PleI	GAGTC	2 cut(s)	430, 910
PpsI	GAGTC	2 cut(s)	430, 910
Ppu21I	YACGTR	1 cut(s)	703
Psp124BI	GAGCTC	1 cut(s)	540
Psp1406I	AACGTT	1 cut(s)	894
Psp6I	CCWGG	4 cut(s)	446, 589, 997, 1066
PspGI	CCWGG	4 cut(s)	446, 589, 997, 1066
PspN4I	GGNNCC	7 cut(s)	65, 373, 505, 511, 888, 949, 1125
PspPI	GGNCC	7 cut(s)	64, 587, 846, 886, 938, 948, 1124
PstI	CTGCAG	1 cut(s)	1258
PstNI	CAGNNNCTG	1 cut(s)	1262
PsyI	GACNNNGTC	1 cut(s)	491
PvuII	CAGCTG	1 cut(s)	1028
RseI	CAYNNNNRTG	4 cut(s)	96, 203, 635, 877
SacI	GAGCTC	1 cut(s)	540
SapI	GCTCTTC	1 cut(s)	626
SaqAI	TTAA	2 cut(s)	140, 711
Sau3AI	GATC	6 cut(s)	414, 468, 804, 1074, 1211, 1242
Sau96I	GGNCC	7 cut(s)	64, 587, 846, 886, 938, 948, 1124
SchI	GAGTC	2 cut(s)	431, 911
ScrFI	CCNGG	5 cut(s)	33, 448, 591, 999, 1068
SduI	GDGCHC	2 cut(s)	40, 540
SfaNI	GCATC	3 cut(s)	218, 800, 1024
SfcI	CTRYAG	1 cut(s)	1254
SinI	GGWCC	2 cut(s)	886, 1124
SmiMI	CAYNNNNRTG	4 cut(s)	96, 203, 635, 877
SmlI	CTYRAG	1 cut(s)	139
SmoI	CTYRAG	1 cut(s)	139
SnaBI	TACGTA	1 cut(s)	703
SphI	GCATGC	1 cut(s)	719
Sse9I	AATT	1 cut(s)	17
SsiI	CCGC	6 cut(s)	323, 513, 594, 597, 627, 927
SstI	GAGCTC	1 cut(s)	540
StyD4I	CCNGG	5 cut(s)	31, 446, 589, 997, 1066
StyI	CCWWGG	5 cut(s)	213, 630, 849, 906, 1011
TaaI	ACNGT	1 cut(s)	876
TaiI	ACGT	3 cut(s)	492, 705, 897
TaqI	TCGA	2 cut(s)	273, 408
TasI	AATT	1 cut(s)	17
TauI	GCSGC	3 cut(s)	515, 597, 600
TfiI	GAWTC	3 cut(s)	7, 275, 1259
Tru1I	TTAA	2 cut(s)	140, 711
Tru9I	TTAA	2 cut(s)	140, 711
TscAI	CASTG	2 cut(s)	958, 1191
TseFI	GTSAC	2 cut(s)	835, 929
TseI	GCWGC	7 cut(s)	230, 719, 1025, 1163, 1250, 1253, 1265
Tsp45I	GTSAC	2 cut(s)	835, 929
TspDTI	ATGAA	3 cut(s)	182, 875, 1034
TspGWI	ACGGA	2 cut(s)	340, 571
TspRI	CASTG	2 cut(s)	958, 1191
Tth111I	GACNNNGTC	1 cut(s)	491
Vha464I	CTTAAG	1 cut(s)	139
VpaK11BI	GGWCC	2 cut(s)	886, 1124
XceI	RCATGY	1 cut(s)	719
XcmI	CCANNNNNNNNNTGG	1 cut(s)	847

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.