RLG00000035410

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
Physical Location & Seq
Reverse (-)
66562795 .. 66565143
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035410

Sequence Viewer

Length: 1071 bp
ATGGCTTCCACGCTCGCCGCCTCCTCTCTCTCCACAAAGCTCCAGAAGCCATCTCTCCTCGAATCCTCCTTCCACGGCGTTCAGGTGGCTCGTCAGCCTTTGAAGGTGGTCTCATCCAACGGCCACGGCCTCTCCGTGTCCATGTCCGCTGGTGGCTCCACCAGCGCTTACGATTTGAGCAACTTCAAGTTCGAGCCGATCAAGGAGTCCATCGTCTCCCGGGAGATGACCAGGCGATACATGACTGACATGATCACCTACGCCGATACCGACGTTGTCATCGTCGGTGCCGGTTCCGCAGGGCTATCTTGCGCATGGGAGCTCAGCAAGAACCCCGATGTGCAGGTAGCCATTATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCGTGGCTCGGTGGCCAGCTCTTCTCGGCCATGGTAGTGAGGAAACCAGCACATCACTTCCTCAACGAGTTGGGCATTGACTACGACGAGAAAGATGACTACGTAGTCATTAAGCATGCTGCCCTATTCACCTCAACTATCATGAGCAAGCTCCTTGCCCGTCCCAATGTCAAACTCTTCAACGCCGTAGCAGCTGAGGATTTGATCATCAAAGGTGGAAGAGTTGCTGGTGTTGTCACCAACTGGGCCTTGGTCTCCATGAACCACGACACACAGTCGTGCATGGACCCCAACGTTATGGAGTCCAAGGTTGTGGTAAGCTCATGCGGTCACGATGGGCCAATGGGAGCCACTGGAGTAAAGAGGTTGAGGAGTGTTGGGATGATTGAGAGCGTGCCTGGGATGAAGGCCTTGGACATGAATGCAGCTGAGGATGCCATTGTGAAGCTAACAAGGGAAATCGTGCCTGGAATGATCGTTACTGGCATGGAAGTTGCTGAGATTGATGGCTCTCCAAGAATGGGACCTACATTCGGGGCTATGATGATATCAGGGCAAAAGGCAGCCCATTTGGCATTGAAGGCACTGGGACTTCCCAACGCTCTTGATGGATCATATGTTGGAGGTATCCAGCCAGAGCTGATCTTAGCTGCTGCAGATTCTGCTGCTGAGATTGCTGATGCTTGA

Protein Analysis

357

Amino Acids

37.38

Weight (kDa)

5.63

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 73 - 306 3.8e-113 Thi4 family
DAO PF01266 91 - 137 3.7e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 91 - 127 6.6e-06 FAD binding domain
NAD_binding_8 PF13450 94 - 129 3.6e-07 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 488
Acc16I TGCGCA 1 cut(s) 313
Acc36I ACCTGC 1 cut(s) 334
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 1 cut(s) 386
AciI CCGC 6 cut(s) 18, 147, 297, 378, 381, 711
AclI AACGTT 1 cut(s) 678
AclWI GGATC 1 cut(s) 1003
AcoI YGGCCR 3 cut(s) 121, 397, 411
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 3 cut(s) 380, 905, 917
AgsI TTSAA 4 cut(s) 103, 187, 565, 964
AhdI GACNNNNNGTC 1 cut(s) 658
AjnI CCWGG 3 cut(s) 230, 781, 850
AleI CACNNNNGTG 1 cut(s) 661
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 3 cut(s) 115, 220, 643
AlwI GGATC 1 cut(s) 1003
AlwNI CAGNNNCTG 1 cut(s) 1046
Ama87I CYCGRG 1 cut(s) 219
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 7 cut(s) 121, 127, 397, 411, 630, 722, 792
ApeKI GCWGC 7 cut(s) 503, 575, 809, 947, 1034, 1037, 1049
AspLEI GCGC 3 cut(s) 167, 314, 386
AspS9I GGNCC 4 cut(s) 630, 670, 722, 908
AsuC2I CCSGG 3 cut(s) 220, 221, 375
AsuHPI GGTGA 3 cut(s) 247, 505, 613
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 2 cut(s) 670, 908
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BalI TGGCCA 1 cut(s) 399
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 324
BbvCI CCTCAGC 2 cut(s) 579, 813
BbvI GCAGC 7 cut(s) 490, 587, 821, 959, 1021, 1024, 1036
BccI CCATC 5 cut(s) 58, 218, 713, 884, 986
BceAI ACGGC 4 cut(s) 91, 136, 142, 554
BciT130I CCWGG 3 cut(s) 232, 783, 852
BciVI GTATCC 1 cut(s) 1022
BclI TGATCA 2 cut(s) 252, 588
BcnI CCSGG 3 cut(s) 220, 221, 375
BcoDI GTCTC 3 cut(s) 115, 220, 643
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 334
BfuI GTATCC 1 cut(s) 1022
BglI GCCNNNNNGGC 2 cut(s) 378, 956
BlpI GCTNAGC 1 cut(s) 323
Bme1390I CCNGG 6 cut(s) 220, 221, 232, 375, 783, 852
Bme18I GGWCC 2 cut(s) 670, 908
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 4 cut(s) 630, 670, 722, 908
BmiI GGNNCC 6 cut(s) 157, 289, 295, 672, 733, 909
BmrFI CCNGG 6 cut(s) 220, 221, 232, 375, 783, 852
BmrI ACTGGG 2 cut(s) 637, 980
BmsI GCATC 2 cut(s) 808, 1054
BmuI ACTGGG 2 cut(s) 637, 980
BpmI CTGGAG 2 cut(s) 26, 759
Bpu10I CCTNAGC 2 cut(s) 579, 813
Bpu1102I GCTNAGC 1 cut(s) 323
BpuMI CCSGG 3 cut(s) 220, 221, 375
BsaAI YACGTR 1 cut(s) 487
BsaI GGTCTC 2 cut(s) 115, 643
BsaJI CCNNGG 9 cut(s) 73, 124, 219, 373, 414, 633, 690, 782, 795
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 3 cut(s) 380, 905, 917
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 4 cut(s) 632, 742, 871, 975
BseBI CCWGG 3 cut(s) 232, 783, 852
BseDI CCNNGG 9 cut(s) 73, 124, 219, 373, 414, 633, 690, 782, 795
BseGI GGATG 4 cut(s) 113, 771, 792, 823
BseLI CCNNNNNNNGG 3 cut(s) 380, 905, 917
BseMII CTCAG 5 cut(s) 337, 570, 804, 873, 1044
BseNI ACTGG 4 cut(s) 632, 742, 871, 975
BseRI GAGGAG 3 cut(s) 13, 47, 769
BseXI GCAGC 7 cut(s) 490, 587, 821, 959, 1021, 1024, 1036
BsgI GTGCAG 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 7 cut(s) 123, 129, 399, 413, 632, 724, 794
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 3 cut(s) 220, 291, 375
BslFI GGGAC 3 cut(s) 531, 921, 987
BslI CCNNNNNNNGG 3 cut(s) 380, 905, 917
BsmAI GTCTC 3 cut(s) 115, 220, 643
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 3 cut(s) 531, 921, 987
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 7 cut(s) 123, 129, 399, 413, 632, 724, 794
Bso31I GGTCTC 2 cut(s) 115, 643
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
Bsp1720I GCTNAGC 1 cut(s) 323
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 6 cut(s) 18, 147, 297, 378, 381, 711
BspANI GGCC 7 cut(s) 123, 129, 399, 413, 632, 724, 794
BspCNI CTCAG 5 cut(s) 336, 571, 805, 874, 1045
BspFNI CGCG 1 cut(s) 386
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 6 cut(s) 157, 289, 295, 672, 733, 909
BspMAI CTGCAG 1 cut(s) 1042
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 1003
BspQI GCTCTTC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 115, 643
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 4 cut(s) 632, 742, 871, 975
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 9 cut(s) 73, 124, 219, 373, 414, 633, 690, 782, 795
BssMI GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
BssT1I CCWWGG 4 cut(s) 414, 633, 690, 795
Bst2UI CCWGG 3 cut(s) 232, 783, 852
Bst4CI ACNGT 1 cut(s) 660
Bst6I CTCTTC 3 cut(s) 410, 566, 598
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 5 cut(s) 15, 401, 501, 533, 779
BstDEI CTNAG 6 cut(s) 323, 579, 813, 882, 1030, 1053
BstDSI CCRYGG 3 cut(s) 73, 124, 414
BstF5I GGATG 4 cut(s) 113, 771, 792, 823
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 314, 386
BstKTI GATC 6 cut(s) 201, 255, 591, 861, 998, 1029
BstMAI GTCTC 3 cut(s) 115, 220, 643
BstMBI GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
BstNI CCWGG 3 cut(s) 232, 783, 852
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 6 cut(s) 218, 219, 230, 373, 781, 850
BstSFI CTRYAG 1 cut(s) 1038
BstSNI TACGTA 1 cut(s) 487
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 7 cut(s) 490, 587, 821, 959, 1021, 1024, 1036
BstXI CCANNNNNNTGG 2 cut(s) 682, 697
BsuI GTATCC 1 cut(s) 1022
BsuRI GGCC 7 cut(s) 123, 129, 399, 413, 632, 724, 794
BtgI CCRYGG 3 cut(s) 73, 124, 414
BtsCI GGATG 4 cut(s) 113, 771, 792, 823
BtsIMutI CAGTG 2 cut(s) 735, 968
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 5 cut(s) 15, 401, 501, 533, 779
CaiI CAGNNNCTG 1 cut(s) 1046
CciI TCATGA 1 cut(s) 525
CfoI GCGC 3 cut(s) 167, 314, 386
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 4 cut(s) 630, 670, 722, 908
Cfr9I CCCGGG 1 cut(s) 219
DdeI CTNAG 6 cut(s) 323, 579, 813, 882, 1030, 1053
DpnI GATC 6 cut(s) 200, 254, 590, 860, 997, 1028
DpnII GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
DrdI GACNNNNNNGTC 1 cut(s) 488
DriI GACNNNNNGTC 1 cut(s) 658
DseDI GACNNNNNNGTC 1 cut(s) 488
EaeI YGGCCR 3 cut(s) 121, 397, 411
Eam1104I CTCTTC 3 cut(s) 410, 566, 598
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 3 cut(s) 410, 566, 598
Ecl136II GAGCTC 1 cut(s) 322
Eco105I TACGTA 1 cut(s) 487
Eco130I CCWWGG 4 cut(s) 414, 633, 690, 795
Eco147I AGGCCT 1 cut(s) 794
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 2 cut(s) 115, 643
Eco32I GATATC 1 cut(s) 933
Eco47I GGWCC 2 cut(s) 670, 908
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 908
EcoRII CCWGG 3 cut(s) 230, 781, 850
EcoRV GATATC 1 cut(s) 933
EcoT14I CCWWGG 4 cut(s) 414, 633, 690, 795
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 4 cut(s) 414, 633, 690, 795
Esp3I CGTCTC 1 cut(s) 220
FaqI GGGAC 3 cut(s) 531, 921, 987
FauNDI CATATG 1 cut(s) 1000
FbaI TGATCA 2 cut(s) 252, 588
FokI GGATG 4 cut(s) 100, 778, 799, 830
FriOI GRGCYC 1 cut(s) 324
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 166, 313, 385
GsuI CTGGAG 2 cut(s) 26, 759
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 7 cut(s) 123, 129, 399, 413, 632, 724, 794
HapII CCGG 3 cut(s) 220, 291, 375
HhaI GCGC 3 cut(s) 167, 314, 386
Hin6I GCGC 3 cut(s) 165, 312, 384
HinP1I GCGC 3 cut(s) 165, 312, 384
HinfI GANTC 4 cut(s) 62, 206, 686, 1043
HpaII CCGG 3 cut(s) 220, 291, 375
HphI GGTGA 3 cut(s) 247, 505, 613
Hpy188III TCNNGA 4 cut(s) 43, 526, 716, 989
Hpy99I CGWCG 3 cut(s) 275, 287, 473
HpyAV CCTTC 4 cut(s) 79, 97, 784, 958
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4IV ACGT 3 cut(s) 273, 486, 678
HpyCH4V TGCA 4 cut(s) 343, 666, 809, 1040
HpyF3I CTNAG 6 cut(s) 323, 579, 813, 882, 1030, 1053
HpySE526I ACGT 3 cut(s) 273, 486, 678
HspAI GCGC 3 cut(s) 165, 312, 384
Ksp22I TGATCA 2 cut(s) 252, 588
Kzo9I GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
LguI GCTCTTC 1 cut(s) 410
LmnI GCTCC 5 cut(s) 45, 161, 319, 540, 731
Lsp1109I GCAGC 7 cut(s) 490, 587, 821, 959, 1021, 1024, 1036
LweI GCATC 2 cut(s) 808, 1054
MaeII ACGT 3 cut(s) 273, 486, 678
MaeIII GTNAC 3 cut(s) 619, 713, 862
MalI GATC 6 cut(s) 200, 254, 590, 860, 997, 1028
MboI GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
MboII GAAGA 3 cut(s) 397, 553, 615
MhlI GDGCHC 1 cut(s) 324
MlsI TGGCCA 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 2 cut(s) 215, 695
MmeI TCCRAC 2 cut(s) 141, 985
Mox20I TGGCCA 1 cut(s) 399
MscI TGGCCA 1 cut(s) 399
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 2 cut(s) 419, 661
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 3 cut(s) 149, 578, 812
MspI CCGG 3 cut(s) 220, 291, 375
MspR9I CCNGG 6 cut(s) 220, 221, 232, 375, 783, 852
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 3 cut(s) 232, 783, 852
MvnI CGCG 1 cut(s) 386
NciI CCSGG 3 cut(s) 220, 221, 375
NcoI CCATGG 1 cut(s) 414
NdeI CATATG 1 cut(s) 1000
NdeII GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
NlaIV GGNNCC 6 cut(s) 157, 289, 295, 672, 733, 909
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 619, 713
NsbI TGCGCA 1 cut(s) 313
NspI RCATGY 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 661
PaeI GCATGC 1 cut(s) 503
PagI TCATGA 1 cut(s) 525
PceI AGGCCT 1 cut(s) 794
PciSI GCTCTTC 1 cut(s) 410
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 267, 279
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 2 cut(s) 62, 1043
PflFI GACNNNGTC 1 cut(s) 275
PleI GAGTC 2 cut(s) 214, 694
PpsI GAGTC 2 cut(s) 214, 694
Ppu21I YACGTR 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 908
Psp124BI GAGCTC 1 cut(s) 324
Psp1406I AACGTT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 3 cut(s) 230, 781, 850
PspGI CCWGG 3 cut(s) 230, 781, 850
PspN4I GGNNCC 6 cut(s) 157, 289, 295, 672, 733, 909
PspPI GGNCC 4 cut(s) 630, 670, 722, 908
PspPPI RGGWCCY 1 cut(s) 908
PstI CTGCAG 1 cut(s) 1042
PstNI CAGNNNCTG 1 cut(s) 1046
PsyI GACNNNGTC 1 cut(s) 275
PvuII CAGCTG 2 cut(s) 578, 812
RseI CAYNNNNRTG 2 cut(s) 419, 661
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 495
Sau3AI GATC 6 cut(s) 198, 252, 588, 858, 995, 1026
Sau96I GGNCC 4 cut(s) 630, 670, 722, 908
SchI GAGTC 2 cut(s) 215, 695
ScrFI CCNGG 6 cut(s) 220, 221, 232, 375, 783, 852
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 2 cut(s) 808, 1054
SfcI CTRYAG 1 cut(s) 1038
SinI GGWCC 2 cut(s) 670, 908
SmaI CCCGGG 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 419, 661
SnaBI TACGTA 1 cut(s) 487
SphI GCATGC 1 cut(s) 503
SseBI AGGCCT 1 cut(s) 794
SsiI CCGC 6 cut(s) 18, 147, 297, 378, 381, 711
SstI GAGCTC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 6 cut(s) 218, 219, 230, 373, 781, 850
StyI CCWWGG 4 cut(s) 414, 633, 690, 795
TaaI ACNGT 1 cut(s) 660
TaiI ACGT 3 cut(s) 276, 489, 681
TaqI TCGA 2 cut(s) 60, 192
TauI GCSGC 3 cut(s) 20, 381, 384
TfiI GAWTC 2 cut(s) 62, 1043
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 742, 975
TseFI GTSAC 2 cut(s) 619, 713
TseI GCWGC 7 cut(s) 503, 575, 809, 947, 1034, 1037, 1049
Tsp45I GTSAC 2 cut(s) 619, 713
TspDTI ATGAA 3 cut(s) 659, 803, 818
TspGWI ACGGA 2 cut(s) 124, 355
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 2 cut(s) 742, 975
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 670, 908
XceI RCATGY 1 cut(s) 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmaI CCCGGG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.