Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Reverse (-)

32911866 .. 32914815

Gene Structure 2950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1224800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGGCATCCATGACTTCCACCCTCACCTCATCCTCTCTCACCACCAAGCTCCAGAAGCCCTCCCTCCTCGACTCCTCCTTCCATGGCGCTCCCATGGTCCGCCTTCAGCCGGCCAAGGCGGCCCCTGCCAGCAACGGAGGCCTCTCCGTCTCCATGTCCGCCAACGGCTTGAACCCCTCGTACGACTTGAGCGCCTTCAAGTTCGACCCCATCAAGGAATCCATCGTCTCCCGGGAGATGACCAGGCGCTACATGACTGACATGATCACCTACGCTGACACCGACGTCGTCGTAGTCGGTGCTGGCTCCGCCGGGCTCTCCTGCGCCTATGAGCTCAGCAAGAACCCCGACGTTCAGGTCGCCATCATAGAGCAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCCATGGTTGTGCGGAAACCCGCCCATCTCTTCCTAAACGAGCTCGGCATTGACTACGATGAGCAAGACAACTACGTCGTCATCAAGCACGCCGCTCTATTCACGTCCACCATCATGAGCAAGCTCCTTGCCAGGCCCAACGTGAAGCTCTTCAACGCTGTGGCCGCCGAGGACTTGATCATCAAGGGTGGCAGAGTTGGTGGGGTGGTCACCAACTGGGCTTTGGTCTCAATGAACCACGACACACAGTCGTGCATGGACCCGAACGTCATGGAGGCCAAGGTTGTGGTGAGCTCATGCGGCCATGATGGACCCATGGGAGCCACCGGGGTGAAGAGGTTGAGGAGTGTCGGAATGATTGAGAGCGTTCCTGGGATGAAGGCATTGGACATGAACACCGCTGAGGATGCTATTGTGAAGCTCACAAGGGAGATTGTGCCCGGAATGATCGTTACCGGCATGGAAGTTGCTGAGATTGATGGATCTCCAAGAATGGGACCTACATTCGGAGCCATGATGATCTCAGGGCAAAAGGCAGCTCACTTGGCATTGAAGGCACTGGGACTCCCCAACGCTTTGGATGGATCGTACGTTGGTGGCATCCAGCCGGAGCTGATCCTAGCTGCTGCAGAATCCGCTGAGATTGCCGAGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

360

Amino Acids

37.67

Weight (kDa)

5.43

Isoelectric Point (pI)

39.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	77 - 310	1e-113	Thi4 family
FAD_binding_2	PF00890	95 - 131	9.3e-07	FAD binding domain
DAO	PF01266	95 - 141	3e-08	FAD dependent oxidoreductase
Pyr_redox_2	PF07992	95 - 137	1e-05	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	98 - 152	5.4e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	4 cut(s)	284, 356, 497, 689
AatII	GACGTC	1 cut(s)	288
AccBSI	CCGCTC	1 cut(s)	518
AclWI	GGATC	3 cut(s)	913, 1015, 1033
AcoI	YGGCCR	4 cut(s)	111, 409, 585, 724
AcuI	CTGAAG	1 cut(s)	89
AcyI	GRCGYC	1 cut(s)	285
AfaI	GTAC	2 cut(s)	182, 1013
AfiI	CCNNNNNNNGG	5 cut(s)	109, 214, 392, 917, 929
AgsI	TTSAA	4 cut(s)	172, 199, 577, 976
AhdI	GACNNNNNGTC	1 cut(s)	670
AjiI	CACGTC	1 cut(s)	528
AjnI	CCWGG	3 cut(s)	242, 554, 793
AleI	CACNNNNGTG	2 cut(s)	673, 752
Alw21I	GWGCWC	3 cut(s)	336, 468, 719
Alw26I	GTCTC	3 cut(s)	154, 232, 655
AlwI	GGATC	3 cut(s)	913, 1015, 1033
Ama87I	CYCGRG	1 cut(s)	231
AoxI	GGCC	8 cut(s)	111, 120, 139, 409, 557, 585, 699, 724
ApeKI	GCWGC	3 cut(s)	959, 1046, 1049
Asp700I	GAANNNNTTC	1 cut(s)	572
AspLEI	GCGC	4 cut(s)	89, 194, 249, 326
AspS9I	GGNCC	6 cut(s)	97, 121, 558, 682, 734, 920
AsuC2I	CCSGG	6 cut(s)	232, 233, 313, 387, 751, 864
AsuHPI	GGTGA	6 cut(s)	16, 31, 259, 625, 724, 766
AvaI	CYCGRG	1 cut(s)	231
AvaII	GGWCC	4 cut(s)	97, 682, 734, 920
BaeGI	GKGCMC	1 cut(s)	864
BalI	TGGCCA	1 cut(s)	411
BanII	GRGCYC	4 cut(s)	318, 336, 468, 719
Bbv12I	GWGCWC	3 cut(s)	336, 468, 719
BbvCI	CCTCAGC	1 cut(s)	825
BbvI	GCAGC	3 cut(s)	971, 1033, 1036
BccI	CCATC	8 cut(s)	218, 230, 371, 456, 542, 725, 896, 998
BceAI	ACGGC	1 cut(s)	181
BciT130I	CCWGG	3 cut(s)	244, 556, 795
BclI	TGATCA	2 cut(s)	264, 600
BcnI	CCSGG	6 cut(s)	232, 233, 313, 387, 751, 864
BcoDI	GTCTC	3 cut(s)	154, 232, 655
BfaI	CTAG	1 cut(s)	1043
BfmI	CTRYAG	1 cut(s)	1050
BfoI	RGCGCY	3 cut(s)	90, 195, 250
BglI	GCCNNNNNGGC	2 cut(s)	119, 390
BisI	GCNGC	7 cut(s)	120, 516, 588, 724, 960, 1047, 1050
BlpI	GCTNAGC	1 cut(s)	335
BlsI	GCNGC	7 cut(s)	121, 517, 589, 725, 961, 1048, 1051
Bme1390I	CCNGG	9 cut(s)	232, 233, 244, 313, 387, 556, 751, 795, 864
Bme18I	GGWCC	4 cut(s)	97, 682, 734, 920
BmeRI	GACNNNNNGTC	1 cut(s)	670
BmeT110I	CYCGRG	1 cut(s)	231
BmgBI	CACGTC	1 cut(s)	528
BmgT120I	GGNCC	6 cut(s)	97, 121, 558, 682, 734, 920
BmiI	GGNNCC	7 cut(s)	123, 307, 684, 736, 745, 921, 934
BmrFI	CCNGG	9 cut(s)	232, 233, 244, 313, 387, 556, 751, 795, 864
BmrI	ACTGGG	2 cut(s)	649, 992
BmsI	GCATC	3 cut(s)	14, 820, 1032
BmuI	ACTGGG	2 cut(s)	649, 992
BpmI	CTGGAG	1 cut(s)	35
Bpu10I	CCTNAGC	1 cut(s)	825
Bpu1102I	GCTNAGC	1 cut(s)	335
BpuEI	CTTGAG	1 cut(s)	208
BpuMI	CCSGG	6 cut(s)	232, 233, 313, 387, 751, 864
BsaHI	GRCGYC	1 cut(s)	285
BsaI	GGTCTC	1 cut(s)	655
BsaXI	ACNNNNNCTCC	2 cut(s)	972, 1002
Bsc4I	CCNNNNNNNGG	5 cut(s)	109, 214, 392, 917, 929
Bse118I	RCCGGY	2 cut(s)	109, 878
Bse1I	ACTGG	2 cut(s)	644, 987
BseBI	CCWGG	3 cut(s)	244, 556, 795
BseGI	GGATG	6 cut(s)	5, 29, 804, 835, 1009, 1023
BseLI	CCNNNNNNNGG	5 cut(s)	109, 214, 392, 917, 929
BseMII	CTCAG	5 cut(s)	349, 816, 885, 960, 1053
BseNI	ACTGG	2 cut(s)	644, 987
BseRI	GAGGAG	3 cut(s)	56, 64, 781
BseSI	GKGCMC	1 cut(s)	864
BseXI	GCAGC	3 cut(s)	971, 1033, 1036
BshFI	GGCC	8 cut(s)	113, 122, 141, 411, 559, 587, 701, 726
BsiHKAI	GWGCWC	3 cut(s)	336, 468, 719
BsiHKCI	CYCGRG	1 cut(s)	231
BsiSI	CCGG	8 cut(s)	110, 232, 312, 387, 750, 864, 879, 1031
BsiWI	CGTACG	2 cut(s)	180, 1011
BslFI	GGGAC	2 cut(s)	933, 999
BslI	CCNNNNNNNGG	5 cut(s)	109, 214, 392, 917, 929
BsmAI	GTCTC	3 cut(s)	154, 232, 655
BsmBI	CGTCTC	2 cut(s)	154, 232
BsmFI	GGGAC	2 cut(s)	933, 999
BsnI	GGCC	8 cut(s)	113, 122, 141, 411, 559, 587, 701, 726
Bso31I	GGTCTC	1 cut(s)	655
BsoBI	CYCGRG	1 cut(s)	231
Bsp1286I	GDGCHC	5 cut(s)	318, 336, 468, 719, 864
Bsp143I	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
Bsp1720I	GCTNAGC	1 cut(s)	335
Bsp19I	CCATGG	4 cut(s)	82, 93, 426, 738
BspANI	GGCC	8 cut(s)	113, 122, 141, 411, 559, 587, 701, 726
BspCNI	CTCAG	5 cut(s)	348, 817, 886, 959, 1054
BspHI	TCATGA	1 cut(s)	537
BspLI	GGNNCC	7 cut(s)	123, 307, 684, 736, 745, 921, 934
BspMAI	CTGCAG	1 cut(s)	1054
BspPI	GGATC	3 cut(s)	913, 1015, 1033
BspQI	GCTCTTC	2 cut(s)	422, 578
BspTNI	GGTCTC	1 cut(s)	655
BsrBI	CCGCTC	1 cut(s)	518
BsrFI	RCCGGY	2 cut(s)	109, 878
BsrI	ACTGG	2 cut(s)	644, 987
BssAI	RCCGGY	2 cut(s)	109, 878
BssMI	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
BssNI	GRCGYC	1 cut(s)	285
BssT1I	CCWWGG	6 cut(s)	82, 93, 114, 426, 702, 738
Bst2UI	CCWGG	3 cut(s)	244, 556, 795
Bst4CI	ACNGT	1 cut(s)	672
Bst6I	CTCTTC	4 cut(s)	422, 458, 578, 752
BstACI	GRCGYC	1 cut(s)	285
BstC8I	GCNNGC	6 cut(s)	111, 130, 304, 413, 513, 545
BstDEI	CTNAG	5 cut(s)	335, 825, 894, 946, 1062
BstDSI	CCRYGG	4 cut(s)	82, 93, 426, 738
BstEII	GGTNACC	1 cut(s)	631
BstF5I	GGATG	6 cut(s)	5, 29, 804, 835, 1009, 1023
BstH2I	RGCGCY	3 cut(s)	90, 195, 250
BstHHI	GCGC	4 cut(s)	89, 194, 249, 326
BstKTI	GATC	7 cut(s)	267, 603, 873, 908, 945, 1010, 1041
BstMAI	GTCTC	3 cut(s)	154, 232, 655
BstMBI	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
BstNI	CCWGG	3 cut(s)	244, 556, 795
BstPI	GGTNACC	1 cut(s)	631
BstSCI	CCNGG	9 cut(s)	230, 231, 242, 311, 385, 554, 749, 793, 862
BstSFI	CTRYAG	1 cut(s)	1050
BstSLI	GKGCMC	1 cut(s)	864
BstV1I	GCAGC	3 cut(s)	971, 1033, 1036
BstX2I	RGATCY	1 cut(s)	905
BstXI	CCANNNNNNTGG	2 cut(s)	709, 1000
BstYI	RGATCY	1 cut(s)	905
BsuRI	GGCC	8 cut(s)	113, 122, 141, 411, 559, 587, 701, 726
BtgI	CCRYGG	4 cut(s)	82, 93, 426, 738
BtrI	CACGTC	1 cut(s)	528
BtsCI	GGATG	6 cut(s)	5, 29, 804, 835, 1009, 1023
BtsIMutI	CAGTG	1 cut(s)	980
Cac8I	GCNNGC	6 cut(s)	111, 130, 304, 413, 513, 545
CciI	TCATGA	1 cut(s)	537
CfoI	GCGC	4 cut(s)	89, 194, 249, 326
Cfr10I	RCCGGY	2 cut(s)	109, 878
Cfr13I	GGNCC	6 cut(s)	97, 121, 558, 682, 734, 920
Cfr9I	CCCGGG	1 cut(s)	231
Csp6I	GTAC	2 cut(s)	181, 1012
CviQI	GTAC	2 cut(s)	181, 1012
DdeI	CTNAG	5 cut(s)	335, 825, 894, 946, 1062
DpnI	GATC	7 cut(s)	266, 602, 872, 907, 944, 1009, 1040
DpnII	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
DrdI	GACNNNNNNGTC	4 cut(s)	284, 356, 497, 689
DriI	GACNNNNNGTC	1 cut(s)	670
DseDI	GACNNNNNNGTC	4 cut(s)	284, 356, 497, 689
EaeI	YGGCCR	4 cut(s)	111, 409, 585, 724
Eam1104I	CTCTTC	4 cut(s)	422, 458, 578, 752
Eam1105I	GACNNNNNGTC	1 cut(s)	670
EarI	CTCTTC	4 cut(s)	422, 458, 578, 752
EciI	GGCGGA	3 cut(s)	89, 148, 298
Ecl136II	GAGCTC	3 cut(s)	334, 466, 717
Eco130I	CCWWGG	6 cut(s)	82, 93, 114, 426, 702, 738
Eco147I	AGGCCT	1 cut(s)	141
Eco24I	GRGCYC	4 cut(s)	318, 336, 468, 719
Eco31I	GGTCTC	1 cut(s)	655
Eco47I	GGWCC	4 cut(s)	97, 682, 734, 920
Eco53kI	GAGCTC	3 cut(s)	334, 466, 717
Eco57I	CTGAAG	1 cut(s)	89
Eco88I	CYCGRG	1 cut(s)	231
Eco91I	GGTNACC	1 cut(s)	631
EcoICRI	GAGCTC	3 cut(s)	334, 466, 717
EcoO109I	RGGNCCY	1 cut(s)	920
EcoO65I	GGTNACC	1 cut(s)	631
EcoRII	CCWGG	3 cut(s)	242, 554, 793
EcoT14I	CCWWGG	6 cut(s)	82, 93, 114, 426, 702, 738
EcoT38I	GRGCYC	4 cut(s)	318, 336, 468, 719
ErhI	CCWWGG	6 cut(s)	82, 93, 114, 426, 702, 738
Esp3I	CGTCTC	2 cut(s)	154, 232
FaqI	GGGAC	2 cut(s)	933, 999
FauI	CCCGC	1 cut(s)	451
FbaI	TGATCA	2 cut(s)	264, 600
Fnu4HI	GCNGC	7 cut(s)	120, 516, 588, 724, 960, 1047, 1050
FokI	GGATG	5 cut(s)	16, 811, 842, 1010, 1016
FriOI	GRGCYC	4 cut(s)	318, 336, 468, 719
Fsp4HI	GCNGC	7 cut(s)	120, 516, 588, 724, 960, 1047, 1050
FspBI	CTAG	1 cut(s)	1043
GlaI	GCGC	4 cut(s)	88, 193, 248, 325
GluI	GCNGC	7 cut(s)	120, 516, 588, 724, 960, 1047, 1050
GsuI	CTGGAG	1 cut(s)	35
HaeII	RGCGCY	3 cut(s)	90, 195, 250
HaeIII	GGCC	8 cut(s)	113, 122, 141, 411, 559, 587, 701, 726
HapII	CCGG	8 cut(s)	110, 232, 312, 387, 750, 864, 879, 1031
HhaI	GCGC	4 cut(s)	89, 194, 249, 326
Hin1I	GRCGYC	1 cut(s)	285
Hin6I	GCGC	4 cut(s)	87, 192, 247, 324
HinP1I	GCGC	4 cut(s)	87, 192, 247, 324
HinfI	GANTC	4 cut(s)	71, 218, 987, 1055
HpaII	CCGG	8 cut(s)	110, 232, 312, 387, 750, 864, 879, 1031
HphI	GGTGA	6 cut(s)	16, 31, 259, 625, 724, 766
Hpy166II	GTNNAC	1 cut(s)	531
Hpy188I	TCNGA	2 cut(s)	776, 932
Hpy188III	TCNNGA	2 cut(s)	52, 538
Hpy8I	GTNNAC	1 cut(s)	531
Hpy99I	CGWCG	5 cut(s)	287, 290, 293, 353, 503
HpyAV	CCTTC	5 cut(s)	88, 113, 205, 796, 970
HpyCH4III	ACNGT	1 cut(s)	672
HpyCH4IV	ACGT	7 cut(s)	285, 351, 498, 527, 564, 690, 1014
HpyCH4V	TGCA	3 cut(s)	398, 678, 1052
HpyF3I	CTNAG	5 cut(s)	335, 825, 894, 946, 1062
HpySE526I	ACGT	7 cut(s)	285, 351, 498, 527, 564, 690, 1014
Hsp92I	GRCGYC	1 cut(s)	285
HspAI	GCGC	4 cut(s)	87, 192, 247, 324
KroI	GCCGGC	1 cut(s)	109
KroNI	GCCGGC	1 cut(s)	111
Ksp22I	TGATCA	2 cut(s)	264, 600
Kzo9I	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
LguI	GCTCTTC	2 cut(s)	422, 578
LmnI	GCTCC	7 cut(s)	54, 94, 311, 552, 743, 932, 1033
Lsp1109I	GCAGC	3 cut(s)	971, 1033, 1036
LweI	GCATC	3 cut(s)	14, 820, 1032
MaeI	CTAG	1 cut(s)	1043
MaeII	ACGT	7 cut(s)	285, 351, 498, 527, 564, 690, 1014
MaeIII	GTNAC	2 cut(s)	631, 874
MalI	GATC	7 cut(s)	266, 602, 872, 907, 944, 1009, 1040
MbiI	CCGCTC	1 cut(s)	518
MboI	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
MboII	GAAGA	4 cut(s)	409, 445, 565, 769
MflI	RGATCY	1 cut(s)	905
MhlI	GDGCHC	5 cut(s)	318, 336, 468, 719, 864
MlsI	TGGCCA	1 cut(s)	411
MluNI	TGGCCA	1 cut(s)	411
MlyI	GAGTC	2 cut(s)	65, 981
MmeI	TCCRAC	1 cut(s)	754
Mox20I	TGGCCA	1 cut(s)	411
MroNI	GCCGGC	1 cut(s)	109
MroXI	GAANNNNTTC	1 cut(s)	572
MscI	TGGCCA	1 cut(s)	411
MseI	TTAA	1 cut(s)	1078
MslI	CAYNNNNRTG	4 cut(s)	431, 536, 673, 752
Msp20I	TGGCCA	1 cut(s)	411
MspA1I	CMGCKG	2 cut(s)	824, 1061
MspI	CCGG	8 cut(s)	110, 232, 312, 387, 750, 864, 879, 1031
MspR9I	CCNGG	9 cut(s)	232, 233, 244, 313, 387, 556, 751, 795, 864
MvaI	CCWGG	3 cut(s)	244, 556, 795
NaeI	GCCGGC	1 cut(s)	111
NciI	CCSGG	6 cut(s)	232, 233, 313, 387, 751, 864
NcoI	CCATGG	4 cut(s)	82, 93, 426, 738
NdeII	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
NgoMIV	GCCGGC	1 cut(s)	109
NlaIV	GGNNCC	7 cut(s)	123, 307, 684, 736, 745, 921, 934
NmeAIII	GCCGAG	2 cut(s)	447, 616
NmuCI	GTSAC	1 cut(s)	631
OliI	CACNNNNGTG	2 cut(s)	673, 752
PagI	TCATGA	1 cut(s)	537
PceI	AGGCCT	1 cut(s)	141
PciSI	GCTCTTC	2 cut(s)	422, 578
PcsI	WCGNNNNNNNCGW	1 cut(s)	279
PdiI	GCCGGC	1 cut(s)	111
PdmI	GAANNNNTTC	1 cut(s)	572
PfeI	GAWTC	2 cut(s)	218, 1055
Pfl23II	CGTACG	2 cut(s)	180, 1011
PflFI	GACNNNGTC	1 cut(s)	287
PkrI	GCNGC	7 cut(s)	121, 517, 589, 725, 961, 1048, 1051
PleI	GAGTC	2 cut(s)	65, 981
PpsI	GAGTC	2 cut(s)	65, 981
PpuMI	RGGWCCY	1 cut(s)	920
Psp124BI	GAGCTC	3 cut(s)	336, 468, 719
Psp5II	RGGWCCY	1 cut(s)	920
Psp6I	CCWGG	3 cut(s)	242, 554, 793
PspEI	GGTNACC	1 cut(s)	631
PspGI	CCWGG	3 cut(s)	242, 554, 793
PspLI	CGTACG	2 cut(s)	180, 1011
PspN4I	GGNNCC	7 cut(s)	123, 307, 684, 736, 745, 921, 934
PspPI	GGNCC	6 cut(s)	97, 121, 558, 682, 734, 920
PspPPI	RGGWCCY	1 cut(s)	920
PsrI	GAACNNNNNNTAC	2 cut(s)	164, 196
PstI	CTGCAG	1 cut(s)	1054
PsuI	RGATCY	1 cut(s)	905
PsyI	GACNNNGTC	1 cut(s)	287
RsaI	GTAC	2 cut(s)	182, 1013
RsaNI	GTAC	2 cut(s)	181, 1012
RseI	CAYNNNNRTG	4 cut(s)	431, 536, 673, 752
SacI	GAGCTC	3 cut(s)	336, 468, 719
SapI	GCTCTTC	2 cut(s)	422, 578
SaqAI	TTAA	1 cut(s)	1078
SatI	GCNGC	7 cut(s)	120, 516, 588, 724, 960, 1047, 1050
Sau3AI	GATC	7 cut(s)	264, 600, 870, 905, 942, 1007, 1038
Sau96I	GGNCC	6 cut(s)	97, 121, 558, 682, 734, 920
SchI	GAGTC	2 cut(s)	65, 981
ScrFI	CCNGG	9 cut(s)	232, 233, 244, 313, 387, 556, 751, 795, 864
SduI	GDGCHC	5 cut(s)	318, 336, 468, 719, 864
SfaNI	GCATC	3 cut(s)	14, 820, 1032
SfcI	CTRYAG	1 cut(s)	1050
SfiI	GGCCNNNNNGGCC	1 cut(s)	119
SinI	GGWCC	4 cut(s)	97, 682, 734, 920
SmaI	CCCGGG	1 cut(s)	233
SmiMI	CAYNNNNRTG	4 cut(s)	431, 536, 673, 752
SmlI	CTYRAG	1 cut(s)	187
SmoI	CTYRAG	1 cut(s)	187
SseBI	AGGCCT	1 cut(s)	141
SspMI	CTAG	1 cut(s)	1043
SstI	GAGCTC	3 cut(s)	336, 468, 719
StuI	AGGCCT	1 cut(s)	141
StyD4I	CCNGG	9 cut(s)	230, 231, 242, 311, 385, 554, 749, 793, 862
StyI	CCWWGG	6 cut(s)	82, 93, 114, 426, 702, 738
TaaI	ACNGT	1 cut(s)	672
TaiI	ACGT	7 cut(s)	288, 354, 501, 530, 567, 693, 1017
TaqI	TCGA	2 cut(s)	69, 204
TauI	GCSGC	4 cut(s)	122, 518, 590, 726
TfiI	GAWTC	2 cut(s)	218, 1055
Tru1I	TTAA	1 cut(s)	1078
Tru9I	TTAA	1 cut(s)	1078
TscAI	CASTG	1 cut(s)	987
TseFI	GTSAC	1 cut(s)	631
TseI	GCWGC	3 cut(s)	959, 1046, 1049
Tsp45I	GTSAC	1 cut(s)	631
TspDTI	ATGAA	3 cut(s)	671, 815, 830
TspGWI	ACGGA	3 cut(s)	136, 150, 367
TspMI	CCCGGG	1 cut(s)	231
TspRI	CASTG	1 cut(s)	987
Tth111I	GACNNNGTC	1 cut(s)	287
VpaK11BI	GGWCC	4 cut(s)	97, 682, 734, 920
XcmI	CCANNNNNNNNNTGG	1 cut(s)	643
XmaI	CCCGGG	1 cut(s)	231
XmnI	GAANNNNTTC	1 cut(s)	572
XspI	CTAG	1 cut(s)	1043
ZraI	GACGTC	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.