Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G54770 |
| fragaria_vesca | FvH4_3g21940 FvH4_3g33490 |
| malus_domestica | MD03G1210100.v1.1 MD11G1224800.v1.1 |
| prunus_persica | Prupe.4G205100_v2.0.a1 |
| pyrus_communis | pycom03g15900 |
| rosa_chinensis | RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911 |
| rosa_laevigata | RLG00000033809 RLG00000035410 |
| rosa_multiflora | Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00019770 Rroxscaffold_1G00043140 |
| rosa_rugosa | Rorug05G0167100 Rorug05G0334800 |
| rosa_samantha | Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000 |
| rosa_wichuraiana | Rw5G023770 Rw5G037510 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 654 |
| AccB1I | GGYRCC | 1 cut(s) | 463 |
| AccBSI | CCGCTC | 1 cut(s) | 593 |
| AciI | CCGC | 3 cut(s) | 140, 593, 970 |
| AclI | AACGTT | 1 cut(s) | 911 |
| AcsI | RAATTY | 4 cut(s) | 356, 826, 916, 1089 |
| AcuI | CTGAAG | 2 cut(s) | 900, 1080 |
| AdeI | CACNNNGTG | 1 cut(s) | 562 |
| AfaI | GTAC | 1 cut(s) | 541 |
| AfeI | AGCGCT | 1 cut(s) | 1279 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1158, 1190 |
| AgsI | TTSAA | 8 cut(s) | 211, 490, 785, 916, 1060, 1072, 1112, 1173 |
| AluBI | AGCT | 4 cut(s) | 86, 458, 635, 1208 |
| AluI | AGCT | 4 cut(s) | 86, 458, 635, 1208 |
| Alw26I | GTCTC | 2 cut(s) | 129, 707 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 20 |
| Ama87I | CYCGRG | 2 cut(s) | 1009, 1183 |
| Aor51HI | AGCGCT | 1 cut(s) | 1279 |
| ApeKI | GCWGC | 3 cut(s) | 427, 891, 1388 |
| ApoI | RAATTY | 4 cut(s) | 356, 826, 916, 1089 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 811, 843 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1093 |
| AspLEI | GCGC | 2 cut(s) | 1196, 1280 |
| AspS9I | GGNCC | 1 cut(s) | 168 |
| AsuC2I | CCSGG | 2 cut(s) | 1184, 1185 |
| AsuHPI | GGTGA | 4 cut(s) | 701, 844, 995, 1235 |
| AvaI | CYCGRG | 2 cut(s) | 1009, 1183 |
| AvaII | GGWCC | 1 cut(s) | 168 |
| BanI | GGYRCC | 1 cut(s) | 463 |
| BbsI | GAAGAC | 1 cut(s) | 798 |
| BbvI | GCAGC | 3 cut(s) | 439, 903, 1400 |
| BccI | CCATC | 4 cut(s) | 475, 1156, 1184, 1214 |
| BciVI | GTATCC | 1 cut(s) | 317 |
| BclI | TGATCA | 2 cut(s) | 3, 1225 |
| BcnI | CCSGG | 2 cut(s) | 1184, 1185 |
| BcoDI | GTCTC | 2 cut(s) | 129, 707 |
| BfaI | CTAG | 2 cut(s) | 497, 636 |
| BfmI | CTRYAG | 1 cut(s) | 91 |
| BfoI | RGCGCY | 1 cut(s) | 1281 |
| BfuI | GTATCC | 1 cut(s) | 317 |
| BisI | GCNGC | 3 cut(s) | 428, 892, 1389 |
| BlsI | GCNGC | 3 cut(s) | 429, 893, 1390 |
| BmcAI | AGTACT | 1 cut(s) | 541 |
| Bme1390I | CCNGG | 2 cut(s) | 1184, 1185 |
| Bme18I | GGWCC | 1 cut(s) | 168 |
| BmeT110I | CYCGRG | 2 cut(s) | 1009, 1183 |
| BmgT120I | GGNCC | 1 cut(s) | 168 |
| BmiI | GGNNCC | 2 cut(s) | 465, 1164 |
| BmrFI | CCNGG | 2 cut(s) | 1184, 1185 |
| BmsI | GCATC | 2 cut(s) | 127, 391 |
| BpiI | GAAGAC | 1 cut(s) | 798 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 652, 684, 1399, 1431 |
| BpmI | CTGGAG | 1 cut(s) | 1327 |
| BpuEI | CTTGAG | 2 cut(s) | 752, 818 |
| BpuMI | CCSGG | 2 cut(s) | 1184, 1185 |
| BsaBI | GATNNNNATC | 2 cut(s) | 252, 1224 |
| BsaJI | CCNNGG | 3 cut(s) | 451, 1183, 1285 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 456, 486, 1300, 1330 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1158, 1190 |
| Bse1I | ACTGG | 1 cut(s) | 943 |
| Bse8I | GATNNNNATC | 2 cut(s) | 252, 1224 |
| BseDI | CCNNGG | 3 cut(s) | 451, 1183, 1285 |
| BseGI | GGATG | 5 cut(s) | 142, 685, 808, 1127, 1148 |
| BseJI | GATNNNNATC | 2 cut(s) | 252, 1224 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1158, 1190 |
| BseMII | CTCAG | 1 cut(s) | 910 |
| BseNI | ACTGG | 1 cut(s) | 943 |
| BseRI | GAGGAG | 1 cut(s) | 580 |
| BseXI | GCAGC | 3 cut(s) | 439, 903, 1400 |
| BshNI | GGYRCC | 1 cut(s) | 463 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1009, 1183 |
| BsiSI | CCGG | 1 cut(s) | 1184 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1158, 1190 |
| BsmAI | GTCTC | 2 cut(s) | 129, 707 |
| BsoBI | CYCGRG | 2 cut(s) | 1009, 1183 |
| Bsp143I | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| Bsp19I | CCATGG | 1 cut(s) | 451 |
| BspACI | CCGC | 3 cut(s) | 140, 593, 970 |
| BspCNI | CTCAG | 1 cut(s) | 909 |
| BspLI | GGNNCC | 2 cut(s) | 465, 1164 |
| BspT107I | GGYRCC | 1 cut(s) | 463 |
| BsrBI | CCGCTC | 1 cut(s) | 593 |
| BsrI | ACTGG | 1 cut(s) | 943 |
| BssECI | CCNNGG | 3 cut(s) | 451, 1183, 1285 |
| BssMI | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| BssT1I | CCWWGG | 1 cut(s) | 451 |
| Bst4CI | ACNGT | 1 cut(s) | 1177 |
| Bst6I | CTCTTC | 3 cut(s) | 39, 705, 1039 |
| BstC8I | GCNNGC | 1 cut(s) | 380 |
| BstDEI | CTNAG | 4 cut(s) | 239, 311, 862, 896 |
| BstDSI | CCRYGG | 1 cut(s) | 451 |
| BstF5I | GGATG | 5 cut(s) | 142, 685, 808, 1127, 1148 |
| BstH2I | RGCGCY | 1 cut(s) | 1281 |
| BstHHI | GCGC | 2 cut(s) | 1196, 1280 |
| BstKTI | GATC | 4 cut(s) | 6, 337, 991, 1228 |
| BstMAI | GTCTC | 2 cut(s) | 129, 707 |
| BstMBI | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1394 |
| BstNSI | RCATGY | 1 cut(s) | 382 |
| BstSCI | CCNGG | 2 cut(s) | 1182, 1183 |
| BstSFI | CTRYAG | 1 cut(s) | 91 |
| BstV1I | GCAGC | 3 cut(s) | 439, 903, 1400 |
| BstV2I | GAAGAC | 1 cut(s) | 798 |
| BsuI | GTATCC | 1 cut(s) | 317 |
| BtgI | CCRYGG | 1 cut(s) | 451 |
| BtsCI | GGATG | 5 cut(s) | 142, 685, 808, 1127, 1148 |
| BtsIMutI | CAGTG | 1 cut(s) | 18 |
| Cac8I | GCNNGC | 1 cut(s) | 380 |
| CaiI | CAGNNNCTG | 1 cut(s) | 20 |
| CfoI | GCGC | 2 cut(s) | 1196, 1280 |
| Cfr13I | GGNCC | 1 cut(s) | 168 |
| Cfr9I | CCCGGG | 1 cut(s) | 1183 |
| Csp6I | GTAC | 1 cut(s) | 540 |
| CviAII | CATG | 5 cut(s) | 379, 438, 452, 519, 1341 |
| CviQI | GTAC | 1 cut(s) | 540 |
| DdeI | CTNAG | 4 cut(s) | 239, 311, 862, 896 |
| DpnI | GATC | 4 cut(s) | 5, 336, 990, 1227 |
| DpnII | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| DraIII | CACNNNGTG | 1 cut(s) | 562 |
| Eam1104I | CTCTTC | 3 cut(s) | 39, 705, 1039 |
| EarI | CTCTTC | 3 cut(s) | 39, 705, 1039 |
| Eco130I | CCWWGG | 1 cut(s) | 451 |
| Eco47I | GGWCC | 1 cut(s) | 168 |
| Eco47III | AGCGCT | 1 cut(s) | 1279 |
| Eco57I | CTGAAG | 2 cut(s) | 900, 1080 |
| Eco88I | CYCGRG | 2 cut(s) | 1009, 1183 |
| EcoRI | GAATTC | 1 cut(s) | 1089 |
| EcoT14I | CCWWGG | 1 cut(s) | 451 |
| ErhI | CCWWGG | 1 cut(s) | 451 |
| FaeI | CATG | 5 cut(s) | 382, 441, 455, 522, 1344 |
| FalI | AAGNNNNNCTT | 5 cut(s) | 1018, 1050, 1078, 1110, 1417 |
| FatI | CATG | 5 cut(s) | 378, 437, 451, 518, 1340 |
| FbaI | TGATCA | 2 cut(s) | 3, 1225 |
| Fnu4HI | GCNGC | 3 cut(s) | 428, 892, 1389 |
| FokI | GGATG | 5 cut(s) | 149, 692, 815, 1114, 1135 |
| Fsp4HI | GCNGC | 3 cut(s) | 428, 892, 1389 |
| FspBI | CTAG | 2 cut(s) | 497, 636 |
| GlaI | GCGC | 2 cut(s) | 1195, 1279 |
| GluI | GCNGC | 3 cut(s) | 428, 892, 1389 |
| GsuI | CTGGAG | 1 cut(s) | 1327 |
| HaeII | RGCGCY | 1 cut(s) | 1281 |
| HapII | CCGG | 1 cut(s) | 1184 |
| HhaI | GCGC | 2 cut(s) | 1196, 1280 |
| Hin1II | CATG | 5 cut(s) | 382, 441, 455, 522, 1344 |
| Hin6I | GCGC | 2 cut(s) | 1194, 1278 |
| HinP1I | GCGC | 2 cut(s) | 1194, 1278 |
| HincII | GTYRAC | 2 cut(s) | 349, 503 |
| HindII | GTYRAC | 2 cut(s) | 349, 503 |
| HinfI | GANTC | 8 cut(s) | 122, 211, 701, 820, 900, 1018, 1124, 1362 |
| HpaI | GTTAAC | 1 cut(s) | 349 |
| HpaII | CCGG | 1 cut(s) | 1184 |
| HphI | GGTGA | 4 cut(s) | 701, 844, 995, 1235 |
| Hpy166II | GTNNAC | 2 cut(s) | 349, 503 |
| Hpy188I | TCNGA | 4 cut(s) | 216, 232, 825, 899 |
| Hpy188III | TCNNGA | 6 cut(s) | 7, 119, 924, 986, 1083, 1306 |
| Hpy8I | GTNNAC | 2 cut(s) | 349, 503 |
| HpyAV | CCTTC | 7 cut(s) | 39, 333, 403, 496, 720, 860, 1104 |
| HpyCH4III | ACNGT | 1 cut(s) | 1177 |
| HpyCH4IV | ACGT | 1 cut(s) | 911 |
| HpyCH4V | TGCA | 6 cut(s) | 378, 382, 427, 613, 665, 1403 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1394 |
| HpyF3I | CTNAG | 4 cut(s) | 239, 311, 862, 896 |
| HpySE526I | ACGT | 1 cut(s) | 911 |
| Hsp92II | CATG | 5 cut(s) | 382, 441, 455, 522, 1344 |
| HspAI | GCGC | 2 cut(s) | 1194, 1278 |
| Ksp22I | TGATCA | 2 cut(s) | 3, 1225 |
| KspAI | GTTAAC | 1 cut(s) | 349 |
| Kzo9I | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| LmnI | GCTCC | 2 cut(s) | 268, 455 |
| LpnPI | CCDG | 9 cut(s) | 802, 891, 924, 962, 1068, 1083, 1197, 1218, 1291 |
| Lsp1109I | GCAGC | 3 cut(s) | 439, 903, 1400 |
| LweI | GCATC | 2 cut(s) | 127, 391 |
| MaeI | CTAG | 2 cut(s) | 497, 636 |
| MaeII | ACGT | 1 cut(s) | 911 |
| MalI | GATC | 4 cut(s) | 5, 336, 990, 1227 |
| MbiI | CCGCTC | 1 cut(s) | 593 |
| MboI | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| MfeI | CAATTG | 2 cut(s) | 190, 1398 |
| MlyI | GAGTC | 3 cut(s) | 131, 814, 894 |
| MmeI | TCCRAC | 2 cut(s) | 664, 703 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1093 |
| MseI | TTAA | 1 cut(s) | 348 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1153, 1233 |
| MspA1I | CMGCKG | 1 cut(s) | 1208 |
| MspI | CCGG | 1 cut(s) | 1184 |
| MspR9I | CCNGG | 2 cut(s) | 1184, 1185 |
| MunI | CAATTG | 2 cut(s) | 190, 1398 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1394 |
| NciI | CCSGG | 2 cut(s) | 1184, 1185 |
| NcoI | CCATGG | 1 cut(s) | 451 |
| NdeII | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| NlaIII | CATG | 5 cut(s) | 382, 441, 455, 522, 1344 |
| NlaIV | GGNNCC | 2 cut(s) | 465, 1164 |
| NmeAIII | GCCGAG | 1 cut(s) | 1266 |
| NspI | RCATGY | 1 cut(s) | 382 |
| PaeI | GCATGC | 1 cut(s) | 382 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1093 |
| PfeI | GAWTC | 5 cut(s) | 211, 701, 1018, 1124, 1362 |
| PkrI | GCNGC | 3 cut(s) | 429, 893, 1390 |
| PleI | GAGTC | 3 cut(s) | 130, 814, 894 |
| PpsI | GAGTC | 3 cut(s) | 130, 814, 894 |
| PsiI | TTATAA | 1 cut(s) | 654 |
| Psp1406I | AACGTT | 1 cut(s) | 911 |
| PspN4I | GGNNCC | 2 cut(s) | 465, 1164 |
| PspPI | GGNCC | 1 cut(s) | 168 |
| PstNI | CAGNNNCTG | 1 cut(s) | 20 |
| PvuII | CAGCTG | 1 cut(s) | 1208 |
| RsaI | GTAC | 1 cut(s) | 541 |
| RsaNI | GTAC | 1 cut(s) | 540 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1153, 1233 |
| SaqAI | TTAA | 1 cut(s) | 348 |
| SatI | GCNGC | 3 cut(s) | 428, 892, 1389 |
| Sau3AI | GATC | 4 cut(s) | 3, 334, 988, 1225 |
| Sau96I | GGNCC | 1 cut(s) | 168 |
| ScaI | AGTACT | 1 cut(s) | 541 |
| SchI | GAGTC | 3 cut(s) | 131, 814, 894 |
| ScrFI | CCNGG | 2 cut(s) | 1184, 1185 |
| SfaNI | GCATC | 2 cut(s) | 127, 391 |
| SfcI | CTRYAG | 1 cut(s) | 91 |
| SinI | GGWCC | 1 cut(s) | 168 |
| SmaI | CCCGGG | 1 cut(s) | 1185 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1153, 1233 |
| SmlI | CTYRAG | 2 cut(s) | 731, 797 |
| SmoI | CTYRAG | 2 cut(s) | 731, 797 |
| SphI | GCATGC | 1 cut(s) | 382 |
| SsiI | CCGC | 3 cut(s) | 140, 593, 970 |
| SspMI | CTAG | 2 cut(s) | 497, 636 |
| StyD4I | CCNGG | 2 cut(s) | 1182, 1183 |
| StyI | CCWWGG | 1 cut(s) | 451 |
| TaaI | ACNGT | 1 cut(s) | 1177 |
| TaiI | ACGT | 1 cut(s) | 914 |
| TaqI | TCGA | 2 cut(s) | 337, 1360 |
| TatI | WGTACW | 1 cut(s) | 539 |
| TfiI | GAWTC | 5 cut(s) | 211, 701, 1018, 1124, 1362 |
| Tru1I | TTAA | 1 cut(s) | 348 |
| Tru9I | TTAA | 1 cut(s) | 348 |
| TscAI | CASTG | 1 cut(s) | 25 |
| TseI | GCWGC | 3 cut(s) | 427, 891, 1388 |
| TspMI | CCCGGG | 1 cut(s) | 1183 |
| TspRI | CASTG | 1 cut(s) | 25 |
| VpaK11BI | GGWCC | 1 cut(s) | 168 |
| XapI | RAATTY | 4 cut(s) | 356, 826, 916, 1089 |
| XceI | RCATGY | 1 cut(s) | 382 |
| XmaI | CCCGGG | 1 cut(s) | 1183 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1093 |
| XspI | CTAG | 2 cut(s) | 497, 636 |
| ZrmI | AGTACT | 1 cut(s) | 541 |