Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005078

Physical Location & Seq

Genomic Coordinates Reverse (-)

65997530 .. 66000267

Gene Structure 2738 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G037510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGCATCCATGGCTTCCACACTCGCTGCCTCCTCTCTCTCCACCAAGCTCCAGAAGCCATCTCTCCTCGAGTCCTCCTTCCACGGCGTTCCCGTTGCTCGGGTTCAGCCTTTGAAGGCGGTCTCATCCAACGGCCACGGCCTCTCCGTGTCCATGTCCGCTGGTGGCTCCACCAGCGCTTACGATTTGAGCAACTTCAAGTTCGAGCCGATCAAGGAGTCCATCGTCTCCCGAGAGATGACCAGGCGATACATGACTGACATGATCACCTACGCCGATACCGACGTTGTCATCGTCGGTGCCGGCTCCGCAGGGCTATCTTGCGCATGGGAGCTCAGCAAGAACCCCGATGTGCAGGTAGCCATTATTGAGCAGTCCGTCAGCCCCGGCGGCGGGGCGTGGCTCGGTGGCCAGCTCTTCTCCGCCATGGTAGTGAGGAAACCAGCACATCACTTCCTCAACGAGTTGGGTATTGACTACGACGAGAAGGATGACTACGTAGTCATTAAGCATGCTGCCCTATTCACCTCAACTATCATGAGCAAACTCCTTGCCCGCCCCAATGTCAAACTCTTCAACGCCGTAGCAGCTGAGGATTTGATCATCAAGGGTGGAAGAGTTGCTGGTGTTGTCACCAACTGGGCCTTGGTCTCCATGAACCACGACACACAGTCGTGCATGGACCCCAACGTTATGGAGTCCAAGGTTGTGGTAAGCTCATGCGGTCACGATGGGCCAATGGGAGCCACTGGAGTAAAGAGGTTGAGGAGTGTTGGGATGATTGAGAGCGTGCCTGGGATGAAGGCCTTGGACATGAATGCAGCTGAGGATGCCTGGCATGGAAGGCAAAAGGCAGCCCATTTGGCATTGAAGGCACTGGGACTTCCCAACGCTCTTGATGGATCATATGTTGGAGGTATCCAGCCAGAGCTGATCTTAGCTGCTGCAGATTCTGCTGCTGAGATTGCTGATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009110 GO:0009228 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010319 GO:0017144 GO:0018130 GO:0018131 GO:0019438 GO:0019904 GO:0031967 GO:0031975 GO:0033554 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046484 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0051716 GO:0052837 GO:0052838 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Thi4

Protein Analysis

325

Amino Acids

34.17

Weight (kDa)

5.98

Isoelectric Point (pI)

38.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thi4	PF01946	77 - 279	1.2e-95	Thi4 family
DAO	PF01266	95 - 141	3.3e-08	FAD dependent oxidoreductase
FAD_binding_2	PF00890	95 - 131	5.8e-06	FAD binding domain
NAD_binding_8	PF13450	98 - 133	3.1e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54770
fragaria_vesca	FvH4_3g21940 FvH4_3g33490
malus_domestica	MD03G1210100.v1.1 MD11G1224800.v1.1
prunus_persica	Prupe.4G205100_v2.0.a1
pyrus_communis	pycom03g15900
rosa_chinensis	RchiOBHm_Chr5g0037761 RchiOBHm_Chr5g0060861 RchiOBHm_Chr5g0060911
rosa_laevigata	RLG00000033809 RLG00000035410
rosa_multiflora	Rmu_sc0000420.1_g000030 Rmu_sc0005416.1_g000004 Rmu_sc0006558.1_g000042 Rmu_sc0026922.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019770 Rroxscaffold_1G00043140
rosa_rugosa	Rorug05G0167100 Rorug05G0334800
rosa_samantha	Rh5AG256400 Rh5AG397800 Rh5BG258700 Rh5BG410200 Rh5CG291800 Rh5CG435200 Rh5DG268400 Rh5DG425000
rosa_wichuraiana	Rw5G023770 Rw5G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	500
Acc16I	TGCGCA	1 cut(s)	325
Acc36I	ACCTGC	1 cut(s)	346
AccB1I	GGYRCC	1 cut(s)	299
AciI	CCGC	8 cut(s)	119, 159, 309, 390, 393, 423, 556, 723
AclI	AACGTT	1 cut(s)	690
AclWI	GGATC	1 cut(s)	910
AcoI	YGGCCR	2 cut(s)	133, 409
AfeI	AGCGCT	1 cut(s)	178
AfiI	CCNNNNNNNGG	3 cut(s)	99, 392, 393
AgsI	TTSAA	4 cut(s)	115, 199, 577, 871
AhdI	GACNNNNNGTC	1 cut(s)	670
AjnI	CCWGG	3 cut(s)	242, 793, 833
AleI	CACNNNNGTG	1 cut(s)	673
AluBI	AGCT	8 cut(s)	49, 334, 415, 590, 717, 824, 931, 941
AluI	AGCT	8 cut(s)	49, 334, 415, 590, 717, 824, 931, 941
Alw21I	GWGCWC	1 cut(s)	336
Alw26I	GTCTC	3 cut(s)	127, 232, 655
AlwI	GGATC	1 cut(s)	910
AlwNI	CAGNNNCTG	1 cut(s)	953
Ama87I	CYCGRG	3 cut(s)	68, 99, 231
Aor51HI	AGCGCT	1 cut(s)	178
AoxI	GGCC	6 cut(s)	133, 139, 409, 642, 734, 804
ApeKI	GCWGC	8 cut(s)	26, 515, 587, 821, 854, 941, 944, 956
AspLEI	GCGC	2 cut(s)	179, 326
AspS9I	GGNCC	3 cut(s)	642, 682, 734
AsuC2I	CCSGG	1 cut(s)	387
AsuHPI	GGTGA	3 cut(s)	259, 517, 625
AvaI	CYCGRG	3 cut(s)	68, 99, 231
AvaII	GGWCC	1 cut(s)	682
BaeI	ACNNNNGTAYC	2 cut(s)	270, 303
BalI	TGGCCA	1 cut(s)	411
BanI	GGYRCC	1 cut(s)	299
BanII	GRGCYC	1 cut(s)	336
BarI	GAAGNNNNNNTAC	2 cut(s)	479, 511
Bbv12I	GWGCWC	1 cut(s)	336
BbvCI	CCTCAGC	2 cut(s)	591, 825
BbvI	GCAGC	8 cut(s)	13, 502, 599, 833, 866, 928, 931, 943
BccI	CCATC	4 cut(s)	67, 230, 725, 893
BceAI	ACGGC	4 cut(s)	100, 148, 154, 566
BciT130I	CCWGG	3 cut(s)	244, 795, 835
BciVI	GTATCC	1 cut(s)	929
BclI	TGATCA	2 cut(s)	264, 600
BcnI	CCSGG	1 cut(s)	387
BcoDI	GTCTC	3 cut(s)	127, 232, 655
BfmI	CTRYAG	1 cut(s)	945
BfoI	RGCGCY	1 cut(s)	180
BfuAI	ACCTGC	1 cut(s)	346
BfuI	GTATCC	1 cut(s)	929
BglI	GCCNNNNNGGC	2 cut(s)	390, 863
BisI	GCNGC	9 cut(s)	27, 391, 516, 588, 822, 855, 942, 945, 957
BlpI	GCTNAGC	1 cut(s)	335
BlsI	GCNGC	9 cut(s)	28, 392, 517, 589, 823, 856, 943, 946, 958
Bme1390I	CCNGG	4 cut(s)	244, 387, 795, 835
Bme18I	GGWCC	1 cut(s)	682
BmeRI	GACNNNNNGTC	1 cut(s)	670
BmeT110I	CYCGRG	3 cut(s)	68, 99, 231
BmgT120I	GGNCC	3 cut(s)	642, 682, 734
BmiI	GGNNCC	5 cut(s)	169, 301, 307, 684, 745
BmrFI	CCNGG	4 cut(s)	244, 387, 795, 835
BmrI	ACTGGG	2 cut(s)	649, 887
BmsI	GCATC	3 cut(s)	14, 820, 961
BmuI	ACTGGG	2 cut(s)	649, 887
BpmI	CTGGAG	2 cut(s)	35, 771
Bpu10I	CCTNAGC	2 cut(s)	591, 825
Bpu1102I	GCTNAGC	1 cut(s)	335
BpuMI	CCSGG	1 cut(s)	387
BsaAI	YACGTR	1 cut(s)	499
BsaI	GGTCTC	2 cut(s)	127, 655
BsaJI	CCNNGG	9 cut(s)	9, 82, 136, 385, 426, 645, 702, 794, 807
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	3 cut(s)	99, 392, 393
Bse118I	RCCGGY	1 cut(s)	302
Bse1I	ACTGG	3 cut(s)	644, 754, 882
BseBI	CCWGG	3 cut(s)	244, 795, 835
BseDI	CCNNGG	9 cut(s)	9, 82, 136, 385, 426, 645, 702, 794, 807
BseGI	GGATG	6 cut(s)	5, 125, 496, 783, 804, 835
BseLI	CCNNNNNNNGG	3 cut(s)	99, 392, 393
BseMII	CTCAG	4 cut(s)	349, 582, 816, 951
BseNI	ACTGG	3 cut(s)	644, 754, 882
BseRI	GAGGAG	3 cut(s)	22, 56, 781
BseXI	GCAGC	8 cut(s)	13, 502, 599, 833, 866, 928, 931, 943
BsgI	GTGCAG	1 cut(s)	374
BshFI	GGCC	6 cut(s)	135, 141, 411, 644, 736, 806
BshNI	GGYRCC	1 cut(s)	299
BsiHKAI	GWGCWC	1 cut(s)	336
BsiHKCI	CYCGRG	3 cut(s)	68, 99, 231
BsiSI	CCGG	2 cut(s)	303, 387
BslFI	GGGAC	1 cut(s)	894
BslI	CCNNNNNNNGG	3 cut(s)	99, 392, 393
BsmAI	GTCTC	3 cut(s)	127, 232, 655
BsmBI	CGTCTC	1 cut(s)	232
BsmFI	GGGAC	1 cut(s)	894
BsmI	GAATGC	1 cut(s)	823
BsnI	GGCC	6 cut(s)	135, 141, 411, 644, 736, 806
Bso31I	GGTCTC	2 cut(s)	127, 655
BsoBI	CYCGRG	3 cut(s)	68, 99, 231
Bsp1286I	GDGCHC	1 cut(s)	336
Bsp143I	GATC	5 cut(s)	210, 264, 600, 902, 933
Bsp1720I	GCTNAGC	1 cut(s)	335
Bsp19I	CCATGG	2 cut(s)	9, 426
BspACI	CCGC	8 cut(s)	119, 159, 309, 390, 393, 423, 556, 723
BspANI	GGCC	6 cut(s)	135, 141, 411, 644, 736, 806
BspCNI	CTCAG	4 cut(s)	348, 583, 817, 952
BspHI	TCATGA	1 cut(s)	537
BspLI	GGNNCC	5 cut(s)	169, 301, 307, 684, 745
BspMAI	CTGCAG	1 cut(s)	949
BspMI	ACCTGC	1 cut(s)	346
BspPI	GGATC	1 cut(s)	910
BspQI	GCTCTTC	1 cut(s)	422
BspT107I	GGYRCC	1 cut(s)	299
BspTNI	GGTCTC	2 cut(s)	127, 655
BsrFI	RCCGGY	1 cut(s)	302
BsrI	ACTGG	3 cut(s)	644, 754, 882
BssAI	RCCGGY	1 cut(s)	302
BssECI	CCNNGG	9 cut(s)	9, 82, 136, 385, 426, 645, 702, 794, 807
BssMI	GATC	5 cut(s)	210, 264, 600, 902, 933
BssT1I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Bst2UI	CCWGG	3 cut(s)	244, 795, 835
Bst4CI	ACNGT	1 cut(s)	672
Bst6I	CTCTTC	3 cut(s)	422, 578, 610
BstAPI	GCANNNNNTGC	1 cut(s)	953
BstBAI	YACGTR	1 cut(s)	499
BstC8I	GCNNGC	5 cut(s)	304, 413, 513, 556, 791
BstDEI	CTNAG	5 cut(s)	335, 591, 825, 937, 960
BstDSI	CCRYGG	4 cut(s)	9, 82, 136, 426
BstF5I	GGATG	6 cut(s)	5, 125, 496, 783, 804, 835
BstH2I	RGCGCY	1 cut(s)	180
BstHHI	GCGC	2 cut(s)	179, 326
BstKTI	GATC	5 cut(s)	213, 267, 603, 905, 936
BstMAI	GTCTC	3 cut(s)	127, 232, 655
BstMBI	GATC	5 cut(s)	210, 264, 600, 902, 933
BstNI	CCWGG	3 cut(s)	244, 795, 835
BstNSI	RCATGY	1 cut(s)	515
BstSCI	CCNGG	4 cut(s)	242, 385, 793, 833
BstSFI	CTRYAG	1 cut(s)	945
BstSNI	TACGTA	1 cut(s)	499
BstV1I	GCAGC	8 cut(s)	13, 502, 599, 833, 866, 928, 931, 943
BstXI	CCANNNNNNTGG	2 cut(s)	694, 709
BsuI	GTATCC	1 cut(s)	929
BsuRI	GGCC	6 cut(s)	135, 141, 411, 644, 736, 806
BtgI	CCRYGG	4 cut(s)	9, 82, 136, 426
BtsCI	GGATG	6 cut(s)	5, 125, 496, 783, 804, 835
BtsIMutI	CAGTG	2 cut(s)	747, 875
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	5 cut(s)	304, 413, 513, 556, 791
CaiI	CAGNNNCTG	1 cut(s)	953
CciI	TCATGA	1 cut(s)	537
CfoI	GCGC	2 cut(s)	179, 326
Cfr10I	RCCGGY	1 cut(s)	302
Cfr13I	GGNCC	3 cut(s)	642, 682, 734
DdeI	CTNAG	5 cut(s)	335, 591, 825, 937, 960
DpnI	GATC	5 cut(s)	212, 266, 602, 904, 935
DpnII	GATC	5 cut(s)	210, 264, 600, 902, 933
DrdI	GACNNNNNNGTC	1 cut(s)	500
DriI	GACNNNNNGTC	1 cut(s)	670
DseDI	GACNNNNNNGTC	1 cut(s)	500
EaeI	YGGCCR	2 cut(s)	133, 409
Eam1104I	CTCTTC	3 cut(s)	422, 578, 610
Eam1105I	GACNNNNNGTC	1 cut(s)	670
EarI	CTCTTC	3 cut(s)	422, 578, 610
EciI	GGCGGA	1 cut(s)	412
Ecl136II	GAGCTC	1 cut(s)	334
Eco105I	TACGTA	1 cut(s)	499
Eco130I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Eco147I	AGGCCT	1 cut(s)	806
Eco24I	GRGCYC	1 cut(s)	336
Eco31I	GGTCTC	2 cut(s)	127, 655
Eco47I	GGWCC	1 cut(s)	682
Eco47III	AGCGCT	1 cut(s)	178
Eco53kI	GAGCTC	1 cut(s)	334
Eco88I	CYCGRG	3 cut(s)	68, 99, 231
EcoICRI	GAGCTC	1 cut(s)	334
EcoRII	CCWGG	3 cut(s)	242, 793, 833
EcoT14I	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
EcoT38I	GRGCYC	1 cut(s)	336
ErhI	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
Esp3I	CGTCTC	1 cut(s)	232
FaqI	GGGAC	1 cut(s)	894
FauI	CCCGC	2 cut(s)	386, 563
FauNDI	CATATG	1 cut(s)	907
FbaI	TGATCA	2 cut(s)	264, 600
Fnu4HI	GCNGC	9 cut(s)	27, 391, 516, 588, 822, 855, 942, 945, 957
FokI	GGATG	5 cut(s)	112, 503, 790, 811, 842
FriOI	GRGCYC	1 cut(s)	336
Fsp4HI	GCNGC	9 cut(s)	27, 391, 516, 588, 822, 855, 942, 945, 957
FspI	TGCGCA	1 cut(s)	325
GlaI	GCGC	2 cut(s)	178, 325
GluI	GCNGC	9 cut(s)	27, 391, 516, 588, 822, 855, 942, 945, 957
GsuI	CTGGAG	2 cut(s)	35, 771
HaeII	RGCGCY	1 cut(s)	180
HaeIII	GGCC	6 cut(s)	135, 141, 411, 644, 736, 806
HapII	CCGG	2 cut(s)	303, 387
HhaI	GCGC	2 cut(s)	179, 326
Hin6I	GCGC	2 cut(s)	177, 324
HinP1I	GCGC	2 cut(s)	177, 324
HinfI	GANTC	4 cut(s)	71, 218, 698, 950
HpaII	CCGG	2 cut(s)	303, 387
HphI	GGTGA	3 cut(s)	259, 517, 625
Hpy188III	TCNNGA	5 cut(s)	52, 231, 538, 728, 896
Hpy99I	CGWCG	3 cut(s)	287, 299, 485
HpyAV	CCTTC	6 cut(s)	88, 109, 481, 796, 837, 865
HpyCH4III	ACNGT	1 cut(s)	672
HpyCH4IV	ACGT	3 cut(s)	285, 498, 690
HpyCH4V	TGCA	4 cut(s)	355, 678, 821, 947
HpyF3I	CTNAG	5 cut(s)	335, 591, 825, 937, 960
HpySE526I	ACGT	3 cut(s)	285, 498, 690
HspAI	GCGC	2 cut(s)	177, 324
KroI	GCCGGC	1 cut(s)	302
KroNI	GCCGGC	1 cut(s)	304
Ksp22I	TGATCA	2 cut(s)	264, 600
Kzo9I	GATC	5 cut(s)	210, 264, 600, 902, 933
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	5 cut(s)	54, 173, 311, 331, 743
Lsp1109I	GCAGC	8 cut(s)	13, 502, 599, 833, 866, 928, 931, 943
LweI	GCATC	3 cut(s)	14, 820, 961
MaeII	ACGT	3 cut(s)	285, 498, 690
MaeIII	GTNAC	2 cut(s)	631, 725
MalI	GATC	5 cut(s)	212, 266, 602, 904, 935
MboI	GATC	5 cut(s)	210, 264, 600, 902, 933
MboII	GAAGA	3 cut(s)	409, 565, 627
MhlI	GDGCHC	1 cut(s)	336
MlsI	TGGCCA	1 cut(s)	411
MluNI	TGGCCA	1 cut(s)	411
MlyI	GAGTC	3 cut(s)	80, 227, 707
MmeI	TCCRAC	2 cut(s)	153, 892
Mox20I	TGGCCA	1 cut(s)	411
MroNI	GCCGGC	1 cut(s)	302
MscI	TGGCCA	1 cut(s)	411
MseI	TTAA	1 cut(s)	507
MslI	CAYNNNNRTG	2 cut(s)	431, 673
Msp20I	TGGCCA	1 cut(s)	411
MspA1I	CMGCKG	3 cut(s)	161, 590, 824
MspI	CCGG	2 cut(s)	303, 387
MspR9I	CCNGG	4 cut(s)	244, 387, 795, 835
Mva1269I	GAATGC	1 cut(s)	823
MvaI	CCWGG	3 cut(s)	244, 795, 835
NaeI	GCCGGC	1 cut(s)	304
NciI	CCSGG	1 cut(s)	387
NcoI	CCATGG	2 cut(s)	9, 426
NdeI	CATATG	1 cut(s)	907
NdeII	GATC	5 cut(s)	210, 264, 600, 902, 933
NgoMIV	GCCGGC	1 cut(s)	302
NlaIV	GGNNCC	5 cut(s)	169, 301, 307, 684, 745
NmuCI	GTSAC	2 cut(s)	631, 725
NsbI	TGCGCA	1 cut(s)	325
NspI	RCATGY	1 cut(s)	515
OliI	CACNNNNGTG	1 cut(s)	673
PaeI	GCATGC	1 cut(s)	515
PaeR7I	CTCGAG	1 cut(s)	68
PagI	TCATGA	1 cut(s)	537
PceI	AGGCCT	1 cut(s)	806
PciSI	GCTCTTC	1 cut(s)	422
PcsI	WCGNNNNNNNCGW	4 cut(s)	90, 144, 279, 291
PctI	GAATGC	1 cut(s)	823
PdiI	GCCGGC	1 cut(s)	304
PfeI	GAWTC	1 cut(s)	950
PflFI	GACNNNGTC	1 cut(s)	287
PkrI	GCNGC	9 cut(s)	28, 392, 517, 589, 823, 856, 943, 946, 958
PleI	GAGTC	3 cut(s)	79, 226, 706
PpsI	GAGTC	3 cut(s)	79, 226, 706
Ppu21I	YACGTR	1 cut(s)	499
Psp124BI	GAGCTC	1 cut(s)	336
Psp1406I	AACGTT	1 cut(s)	690
Psp6I	CCWGG	3 cut(s)	242, 793, 833
PspGI	CCWGG	3 cut(s)	242, 793, 833
PspN4I	GGNNCC	5 cut(s)	169, 301, 307, 684, 745
PspPI	GGNCC	3 cut(s)	642, 682, 734
PspXI	VCTCGAGB	1 cut(s)	68
PstI	CTGCAG	1 cut(s)	949
PstNI	CAGNNNCTG	1 cut(s)	953
PsyI	GACNNNGTC	1 cut(s)	287
PvuII	CAGCTG	2 cut(s)	590, 824
RseI	CAYNNNNRTG	2 cut(s)	431, 673
SacI	GAGCTC	1 cut(s)	336
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	1 cut(s)	507
SatI	GCNGC	9 cut(s)	27, 391, 516, 588, 822, 855, 942, 945, 957
Sau3AI	GATC	5 cut(s)	210, 264, 600, 902, 933
Sau96I	GGNCC	3 cut(s)	642, 682, 734
SchI	GAGTC	3 cut(s)	80, 227, 707
ScrFI	CCNGG	4 cut(s)	244, 387, 795, 835
SduI	GDGCHC	1 cut(s)	336
SfaNI	GCATC	3 cut(s)	14, 820, 961
SfcI	CTRYAG	1 cut(s)	945
Sfr274I	CTCGAG	1 cut(s)	68
SinI	GGWCC	1 cut(s)	682
SlaI	CTCGAG	1 cut(s)	68
SmiMI	CAYNNNNRTG	2 cut(s)	431, 673
SmlI	CTYRAG	1 cut(s)	68
SmoI	CTYRAG	1 cut(s)	68
SnaBI	TACGTA	1 cut(s)	499
SphI	GCATGC	1 cut(s)	515
SseBI	AGGCCT	1 cut(s)	806
SsiI	CCGC	8 cut(s)	119, 159, 309, 390, 393, 423, 556, 723
SstI	GAGCTC	1 cut(s)	336
StuI	AGGCCT	1 cut(s)	806
StyD4I	CCNGG	4 cut(s)	242, 385, 793, 833
StyI	CCWWGG	5 cut(s)	9, 426, 645, 702, 807
TaaI	ACNGT	1 cut(s)	672
TaiI	ACGT	3 cut(s)	288, 501, 693
TaqI	TCGA	2 cut(s)	69, 204
TauI	GCSGC	1 cut(s)	393
TfiI	GAWTC	1 cut(s)	950
Tru1I	TTAA	1 cut(s)	507
Tru9I	TTAA	1 cut(s)	507
TscAI	CASTG	2 cut(s)	754, 882
TseFI	GTSAC	2 cut(s)	631, 725
TseI	GCWGC	8 cut(s)	26, 515, 587, 821, 854, 941, 944, 956
Tsp45I	GTSAC	2 cut(s)	631, 725
TspDTI	ATGAA	3 cut(s)	671, 815, 830
TspGWI	ACGGA	2 cut(s)	136, 367
TspRI	CASTG	2 cut(s)	754, 882
Tth111I	GACNNNGTC	1 cut(s)	287
VpaK11BI	GGWCC	1 cut(s)	682
XceI	RCATGY	1 cut(s)	515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	643
XhoI	CTCGAG	1 cut(s)	68

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.