AT5G55700

beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008239
Physical Location & Seq
Forward (+)
22551734 .. 22554964
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55700.1

Sequence Viewer

Length: 1596 bp
ATGACGGAGACTGGAGTAATCGGATGTGGCTGTCGCGGAGTCACCGGCGGCAATTTCTTCCATCCCGGAGGATTTTCTTTGAAATCTTGTTTCCTCGAGCAGAGTACAAAGCGTAATCGTAACTTTTTCCGAAGCGTTTCTATGATTCCTCCTTTCAAACGCGGTCGTTTCATCACTAAGTTGCGTTCCGTCGCCGGGAACAGCCGAATCTTTAGCATGGATGCTCGAGAGAAATCACGATCGTTTGTGTTGGTATCATCAAGGCACAAGAGAGTTCCAGTTTTTGTGATGATGCCGATTGATACATTTGGAATTGATGCTTCTGGGTGTCCAAAGATTAAAAGGCTCAAGGCTTTAACTGTATCTCTTAAGGCACTCAAGTTAGCTGGTGTTCATGGAATCGCAGTTGAGGTTTGGTGGGGGATTGTAGAGCGTTTCTCTCCTCTTGAGTTTAAATGGTCACTGTATGAAGAGCTTTTTAGACTGATTTCTGAGGCAGGGTTGAAGTTACATGTTGCTCTTTGCTTTCATTCAAATATGCATTTGTTTGGTGGGAAAGGAGGCATCAGTCTTCCACTCTGGATCCGAGAGATTGGAGACGTCAATAAGGACATATACTATAGAGATAAAAGCGGATTTTCCAACAATGACTATCTCACACTTGGAGTCGATCAACTTCCTTTGTTCGGTGGCCGTACTGCTGTTCAATGCTATGAAGATTTTATGCTCAGTTTTTCAACAAAATTTGAGCCATATCTTGGGAATGTGATTGAAGAAATAAGTATAGGTCTTGGTCCTTCGGGGGAGCTTAGATATCCTGCACATCCTTCTGGAGATGGGAGGTGGAAATTTCCTGGAATTGGTGAATTCCAATGCCATGACAAGTACATGATGGAAGACTTGATGGCAGTGGCATCCCAAGAAGGCAAACCTCAATGGGGAAGCAGAGATCCTCCGAATACCGGCTGCTATAATAGCTTTCCATCTGGAGTTCCGTTCTTTGAGGAGGGCAATGATAGCTTTCTCTCTGACTATGGTCGTTTCTTTCTAGAATGGTACAGTGGGAAGTTGATTTGTCATGCTGATGCTATTCTTGCAAAGGCAGCCGATGTCTTGCGGAGACGTCAGGAAGAAGAGAAAAGCTCTGTAATGCTGGTTGCAAAAATTGGTGGAATCTATTGGTGGTATAAGACATCTTCACACCCCGCTGAACTAACTGCAGGTTATTACAACACCTCCCTCAGGGATGGTTATGATCCTGTAGCTTCCGTCTTGTCTCGTCATGGTGCTGCTCTCAACATCCCCTGCTTGGATATGGCAGATAGTGAAATACCTGAGAAATATCTTTGCAGCCCTGAAGGATTACGTAGACAGATACATGATGTTTCGAAGAAGTGGACAATACATGTGACTGGTAGAAACACAAGCGAAAGATTTGATGAGATGGGACTAAGGCAAATACGAGAGAACTGTGTGCAACCGAATGGCGACACTCTAAGATCATTTACGTTTTGCAGAATGAATGAGAAGATCTTTAGGGTCGAGAACTGGAACAACTTTGTCCCTTTCATTAGACAGATGAGTGCAGATATGTAA

Protein Analysis

531

Amino Acids

60.12

Weight (kDa)

8.74

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 95 - 523 6.2e-109 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 603, 1126
Acc36I ACCTGC 1 cut(s) 1211
AccB7I CCANNNNNTGG 1 cut(s) 758
AccI GTMKAC 1 cut(s) 1369
AccII CGCG 2 cut(s) 36, 162
AciI CCGC 6 cut(s) 36, 48, 162, 633, 1117, 1206
AclWI GGATC 4 cut(s) 577, 590, 944, 1250
AcoI YGGCCR 1 cut(s) 691
AcsI RAATTY 3 cut(s) 743, 848, 866
AcuI CTGAAG 1 cut(s) 1377
AcyI GRCGYC 2 cut(s) 600, 1123
AfaI GTAC 4 cut(s) 106, 697, 887, 1058
AfiI CCNNNNNNNGG 8 cut(s) 195, 686, 758, 860, 938, 962, 1242, 1309
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 2 cut(s) 511, 1405
AgsI TTSAA 7 cut(s) 82, 157, 505, 534, 707, 738, 773
AjnI CCWGG 1 cut(s) 853
AluBI AGCT 7 cut(s) 386, 475, 808, 978, 1020, 1143, 1265
AluI AGCT 7 cut(s) 386, 475, 808, 978, 1020, 1143, 1265
Alw26I GTCTC 4 cut(s) 2, 591, 1114, 1281
AlwI GGATC 4 cut(s) 577, 590, 944, 1250
Ama87I CYCGRG 2 cut(s) 95, 225
AoxI GGCC 1 cut(s) 691
ApeKI GCWGC 4 cut(s) 966, 1103, 1289, 1350
ApoI RAATTY 3 cut(s) 743, 848, 866
Asp700I GAANNNNTTC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 794
AsuC2I CCSGG 2 cut(s) 66, 196
AsuHPI GGTGA 2 cut(s) 34, 875
AsuII TTCGAA 1 cut(s) 1388
AvaI CYCGRG 2 cut(s) 95, 225
AvaII GGWCC 1 cut(s) 794
AxyI CCTNAGG 1 cut(s) 1241
BamHI GGATCC 1 cut(s) 582
BbsI GAAGAC 2 cut(s) 563, 903
BbvI GCAGC 4 cut(s) 953, 1115, 1276, 1362
BccI CCATC 7 cut(s) 69, 830, 886, 898, 991, 1241, 1438
BceAI ACGGC 1 cut(s) 678
BciT130I CCWGG 1 cut(s) 855
BcnI CCSGG 2 cut(s) 66, 196
BcoDI GTCTC 4 cut(s) 2, 591, 1114, 1281
BfaI CTAG 1 cut(s) 1049
BfmI CTRYAG 3 cut(s) 619, 1218, 1260
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 1211
BglII AGATCT 1 cut(s) 1530
BisI GCNGC 5 cut(s) 49, 967, 1104, 1290, 1351
BlsI GCNGC 5 cut(s) 50, 968, 1105, 1291, 1352
Bme1390I CCNGG 3 cut(s) 66, 196, 855
Bme18I GGWCC 1 cut(s) 794
BmeT110I CYCGRG 2 cut(s) 95, 225
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 584
BmrFI CCNGG 3 cut(s) 66, 196, 855
BmsI GCATC 6 cut(s) 211, 282, 307, 573, 923, 1075
BoxI GACNNNNGTC 1 cut(s) 1035
BpiI GAAGAC 2 cut(s) 563, 903
BplI GAGNNNNNCTC 4 cut(s) 422, 454, 1127, 1159
BpmI CTGGAG 3 cut(s) 33, 852, 1008
Bpu14I TTCGAA 1 cut(s) 1388
BpuEI CTTGAG 3 cut(s) 332, 362, 467
BpuMI CCSGG 2 cut(s) 66, 196
BsaAI YACGTR 1 cut(s) 1367
BsaHI GRCGYC 2 cut(s) 600, 1123
BsaXI ACNNNNNCTCC 2 cut(s) 1220, 1250
Bsc4I CCNNNNNNNGG 8 cut(s) 195, 686, 758, 860, 938, 962, 1242, 1309
Bse118I RCCGGY 2 cut(s) 44, 962
Bse1I ACTGG 4 cut(s) 16, 278, 1417, 1553
Bse21I CCTNAGG 1 cut(s) 1241
Bse3DI GCAATG 1 cut(s) 1018
BseBI CCWGG 1 cut(s) 855
BseGI GGATG 7 cut(s) 29, 61, 226, 823, 914, 1252, 1299
BseLI CCNNNNNNNGG 8 cut(s) 195, 686, 758, 860, 938, 962, 1242, 1309
BseMI GCAATG 1 cut(s) 1018
BseMII CTCAG 4 cut(s) 483, 742, 1255, 1326
BseNI ACTGG 4 cut(s) 16, 278, 1417, 1553
BseRI GAGGAG 2 cut(s) 432, 1019
BseXI GCAGC 4 cut(s) 953, 1115, 1276, 1362
BsgI GTGCAG 1 cut(s) 804
Bsh1236I CGCG 2 cut(s) 36, 162
Bsh1285I CGRYCG 2 cut(s) 166, 242
BshFI GGCC 1 cut(s) 693
BsiEI CGRYCG 2 cut(s) 166, 242
BsiHKCI CYCGRG 2 cut(s) 95, 225
BsiSI CCGG 4 cut(s) 45, 66, 195, 963
BslFI GGGAC 2 cut(s) 1461, 1547
BslI CCNNNNNNNGG 8 cut(s) 195, 686, 758, 860, 938, 962, 1242, 1309
BsmAI GTCTC 4 cut(s) 2, 591, 1114, 1281
BsmBI CGTCTC 2 cut(s) 591, 1114
BsmFI GGGAC 2 cut(s) 1461, 1547
BsnI GGCC 1 cut(s) 693
BsoBI CYCGRG 2 cut(s) 95, 225
Bsp119I TTCGAA 1 cut(s) 1388
Bsp143I GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
BspACI CCGC 6 cut(s) 36, 48, 162, 633, 1117, 1206
BspANI GGCC 1 cut(s) 693
BspCNI CTCAG 4 cut(s) 484, 741, 1254, 1327
BspFNI CGCG 2 cut(s) 36, 162
BspLI GGNNCC 1 cut(s) 584
BspMAI CTGCAG 1 cut(s) 1222
BspMI ACCTGC 1 cut(s) 1211
BspPI GGATC 4 cut(s) 577, 590, 944, 1250
BspQI GCTCTTC 1 cut(s) 465
BspT104I TTCGAA 1 cut(s) 1388
BspTI CTTAAG 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 1018
BsrFI RCCGGY 2 cut(s) 44, 962
BsrI ACTGG 4 cut(s) 16, 278, 1417, 1553
BssAI RCCGGY 2 cut(s) 44, 962
BssMI GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
BssNI GRCGYC 2 cut(s) 600, 1123
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 4 cut(s) 361, 465, 1061, 1472
Bst6I CTCTTC 2 cut(s) 465, 1128
BstACI GRCGYC 2 cut(s) 600, 1123
BstAFI CTTAAG 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 1367
BstBI TTCGAA 1 cut(s) 1388
BstDEI CTNAG 8 cut(s) 177, 492, 728, 809, 1241, 1335, 1451, 1496
BstENI CCTNNNNNAGG 1 cut(s) 1240
BstF5I GGATG 7 cut(s) 29, 61, 226, 823, 914, 1252, 1299
BstFNI CGCG 2 cut(s) 36, 162
BstKTI GATC 7 cut(s) 242, 585, 673, 952, 1258, 1502, 1533
BstMAI GTCTC 4 cut(s) 2, 591, 1114, 1281
BstMBI GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
BstMCI CGRYCG 2 cut(s) 166, 242
BstMWI GCNNNNNNNGC 4 cut(s) 975, 1017, 1094, 1103
BstNI CCWGG 1 cut(s) 855
BstNSI RCATGY 2 cut(s) 515, 1409
BstPAI GACNNNNGTC 1 cut(s) 1035
BstSCI CCNGG 3 cut(s) 64, 194, 853
BstSFI CTRYAG 3 cut(s) 619, 1218, 1260
BstSNI TACGTA 1 cut(s) 1367
BstUI CGCG 2 cut(s) 36, 162
BstV1I GCAGC 4 cut(s) 953, 1115, 1276, 1362
BstV2I GAAGAC 2 cut(s) 563, 903
BstX2I RGATCY 3 cut(s) 582, 949, 1530
BstYI RGATCY 3 cut(s) 582, 949, 1530
Bsu36I CCTNAGG 1 cut(s) 1241
BsuRI GGCC 1 cut(s) 693
BtsCI GGATG 7 cut(s) 29, 61, 226, 823, 914, 1252, 1299
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 3 cut(s) 461, 915, 1066
BveI ACCTGC 1 cut(s) 1211
Cfr10I RCCGGY 2 cut(s) 44, 962
Cfr13I GGNCC 1 cut(s) 794
Csp6I GTAC 4 cut(s) 105, 696, 886, 1057
CviAII CATG 9 cut(s) 217, 395, 512, 878, 889, 1079, 1283, 1379, 1406
CviQI GTAC 4 cut(s) 105, 696, 886, 1057
DdeI CTNAG 8 cut(s) 177, 492, 728, 809, 1241, 1335, 1451, 1496
DpnI GATC 7 cut(s) 241, 584, 672, 951, 1257, 1501, 1532
DpnII GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
DraI TTTAAA 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 691
Eam1104I CTCTTC 2 cut(s) 465, 1128
EarI CTCTTC 2 cut(s) 465, 1128
Eco105I TACGTA 1 cut(s) 1367
Eco32I GATATC 1 cut(s) 815
Eco47I GGWCC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 1377
Eco81I CCTNAGG 1 cut(s) 1241
Eco88I CYCGRG 2 cut(s) 95, 225
EcoNI CCTNNNNNAGG 1 cut(s) 1240
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 1 cut(s) 853
EcoRV GATATC 1 cut(s) 815
EcoT22I ATGCAT 1 cut(s) 543
Esp3I CGTCTC 2 cut(s) 591, 1114
FaeI CATG 9 cut(s) 220, 398, 515, 881, 892, 1082, 1286, 1382, 1409
FaqI GGGAC 2 cut(s) 1461, 1547
FatI CATG 9 cut(s) 216, 394, 511, 877, 888, 1078, 1282, 1378, 1405
FauI CCCGC 1 cut(s) 1213
FblI GTMKAC 1 cut(s) 1369
Fnu4HI GCNGC 5 cut(s) 49, 967, 1104, 1290, 1351
FokI GGATG 7 cut(s) 36, 48, 233, 810, 901, 1259, 1286
Fsp4HI GCNGC 5 cut(s) 49, 967, 1104, 1290, 1351
FspBI CTAG 1 cut(s) 1049
GluI GCNGC 5 cut(s) 49, 967, 1104, 1290, 1351
GsuI CTGGAG 3 cut(s) 33, 852, 1008
HaeIII GGCC 1 cut(s) 693
HapII CCGG 4 cut(s) 45, 66, 195, 963
Hin1I GRCGYC 2 cut(s) 600, 1123
Hin1II CATG 9 cut(s) 220, 398, 515, 881, 892, 1082, 1286, 1382, 1409
HinfI GANTC 6 cut(s) 39, 145, 207, 399, 666, 1173
HpaII CCGG 4 cut(s) 45, 66, 195, 963
HphI GGTGA 2 cut(s) 34, 875
Hpy166II GTNNAC 2 cut(s) 1370, 1398
Hpy188I TCNGA 6 cut(s) 23, 131, 493, 587, 957, 1030
Hpy188III TCNNGA 9 cut(s) 227, 237, 446, 580, 831, 987, 1049, 1127, 1543
Hpy8I GTNNAC 2 cut(s) 1370, 1398
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 4 cut(s) 807, 837, 917, 1352
HpyCH4III ACNGT 4 cut(s) 361, 465, 1061, 1472
HpyCH4IV ACGT 4 cut(s) 600, 1123, 1366, 1508
HpyCH4V TGCA 9 cut(s) 541, 821, 1097, 1160, 1220, 1350, 1477, 1515, 1586
HpyF10VI GCNNNNNNNGC 4 cut(s) 975, 1017, 1094, 1103
HpyF3I CTNAG 8 cut(s) 177, 492, 728, 809, 1241, 1335, 1451, 1496
HpySE526I ACGT 4 cut(s) 600, 1123, 1366, 1508
Hsp92I GRCGYC 2 cut(s) 600, 1123
Hsp92II CATG 9 cut(s) 220, 398, 515, 881, 892, 1082, 1286, 1382, 1409
Kzo9I GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 4 cut(s) 953, 1115, 1276, 1362
LweI GCATC 6 cut(s) 211, 282, 307, 573, 923, 1075
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 4 cut(s) 600, 1123, 1366, 1508
MaeIII GTNAC 5 cut(s) 40, 119, 459, 507, 1408
MalI GATC 7 cut(s) 241, 584, 672, 951, 1257, 1501, 1532
MboI GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
MflI RGATCY 3 cut(s) 582, 949, 1530
MluCI AATT 7 cut(s) 52, 312, 743, 848, 858, 866, 1164
MlyI GAGTC 2 cut(s) 48, 675
MmeI TCCRAC 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 136
MseI TTAA 4 cut(s) 339, 356, 369, 453
MslI CAYNNNNRTG 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 1208
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 4 cut(s) 45, 66, 195, 963
MspR9I CCNGG 3 cut(s) 66, 196, 855
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 2 cut(s) 36, 162
MwoI GCNNNNNNNGC 4 cut(s) 975, 1017, 1094, 1103
NciI CCSGG 2 cut(s) 66, 196
NdeII GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
NlaIII CATG 9 cut(s) 220, 398, 515, 881, 892, 1082, 1286, 1382, 1409
NlaIV GGNNCC 1 cut(s) 584
NmuCI GTSAC 3 cut(s) 40, 459, 1408
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 2 cut(s) 515, 1409
NspV TTCGAA 1 cut(s) 1388
PaeR7I CTCGAG 2 cut(s) 95, 225
PciI ACATGT 2 cut(s) 511, 1405
PciSI GCTCTTC 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 136
PfeI GAWTC 4 cut(s) 145, 207, 399, 1173
PflMI CCANNNNNTGG 1 cut(s) 758
PfoI TCCNGGA 2 cut(s) 64, 853
PkrI GCNGC 5 cut(s) 50, 968, 1105, 1291, 1352
Ple19I CGATCG 1 cut(s) 242
PleI GAGTC 2 cut(s) 47, 674
PpsI GAGTC 2 cut(s) 47, 674
Ppu21I YACGTR 1 cut(s) 1367
PscI ACATGT 2 cut(s) 511, 1405
PshAI GACNNNNGTC 1 cut(s) 1035
Psp6I CCWGG 1 cut(s) 853
PspGI CCWGG 1 cut(s) 853
PspN4I GGNNCC 1 cut(s) 584
PspPI GGNCC 1 cut(s) 794
PspXI VCTCGAGB 1 cut(s) 95
PstI CTGCAG 1 cut(s) 1222
PsuI RGATCY 3 cut(s) 582, 949, 1530
PvuI CGATCG 1 cut(s) 242
RsaI GTAC 4 cut(s) 106, 697, 887, 1058
RsaNI GTAC 4 cut(s) 105, 696, 886, 1057
RseI CAYNNNNRTG 1 cut(s) 1083
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 4 cut(s) 339, 356, 369, 453
SatI GCNGC 5 cut(s) 49, 967, 1104, 1290, 1351
Sau3AI GATC 7 cut(s) 239, 582, 670, 949, 1255, 1499, 1530
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 2 cut(s) 48, 675
ScrFI CCNGG 3 cut(s) 66, 196, 855
SfaNI GCATC 6 cut(s) 211, 282, 307, 573, 923, 1075
SfcI CTRYAG 3 cut(s) 619, 1218, 1260
Sfr274I CTCGAG 2 cut(s) 95, 225
SfuI TTCGAA 1 cut(s) 1388
SgrAI CRCCGGYG 1 cut(s) 44
SinI GGWCC 1 cut(s) 794
SlaI CTCGAG 2 cut(s) 95, 225
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 6 cut(s) 95, 225, 347, 368, 377, 446
SmoI CTYRAG 6 cut(s) 95, 225, 347, 368, 377, 446
SnaBI TACGTA 1 cut(s) 1367
Sse9I AATT 7 cut(s) 52, 312, 743, 848, 858, 866, 1164
SsiI CCGC 6 cut(s) 36, 48, 162, 633, 1117, 1206
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 3 cut(s) 64, 194, 853
TaaI ACNGT 4 cut(s) 361, 465, 1061, 1472
TaiI ACGT 4 cut(s) 603, 1126, 1369, 1511
TaqI TCGA 5 cut(s) 96, 226, 669, 1388, 1542
TasI AATT 7 cut(s) 52, 312, 743, 848, 858, 866, 1164
TatI WGTACW 2 cut(s) 104, 885
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 4 cut(s) 145, 207, 399, 1173
Tru1I TTAA 4 cut(s) 339, 356, 369, 453
Tru9I TTAA 4 cut(s) 339, 356, 369, 453
TscAI CASTG 3 cut(s) 468, 915, 1066
TseFI GTSAC 3 cut(s) 40, 459, 1408
TseI GCWGC 4 cut(s) 966, 1103, 1289, 1350
Tsp45I GTSAC 3 cut(s) 40, 459, 1408
TspDTI ATGAA 7 cut(s) 160, 383, 483, 518, 729, 1535, 1558
TspGWI ACGGA 4 cut(s) 20, 178, 984, 1258
TspRI CASTG 3 cut(s) 468, 915, 1066
Van91I CCANNNNNTGG 1 cut(s) 758
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 794
XagI CCTNNNNNAGG 1 cut(s) 1240
XapI RAATTY 3 cut(s) 743, 848, 866
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 2 cut(s) 515, 1409
XhoI CTCGAG 2 cut(s) 95, 225
XmiI GTMKAC 1 cut(s) 1369
XmnI GAANNNNTTC 1 cut(s) 136
XspI CTAG 1 cut(s) 1049
ZraI GACGTC 2 cut(s) 601, 1124
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.