MD11G1199800.v1.1
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008239
Physical Location & Seq
Reverse (-)
28836952 .. 28839212
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1199800.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCAATGGCGGACAATGGGCGAGTGGTGTGCAAATGCACGGCAGCCAGGAGGAGCTTCTTCTGCTTCAGAGAAGTCAGCTTCGATGACAAGCCCAACCGCCGAAACCACCTCCGGAACGTTTCAGCCGTTCCCGTCTTTAAAAACGGCATCTTCACTCGCTGCAGCCCATCTGTCGCCGGAAACAGCATTCTCAGTATGGATGCACGTGAGAATCCGAGGTCCTCGATATTGCCATCATCCCGAGATAAGAGAATTCCCATATATGTGATGATGCCTGTTGATGCTTTCTGCATTGATGGTTCGGGGCGTCCAAGGATTAGGAAAATCAAGGCCTTAACTGTAGCTCTTAAAGCACTCAAGTTGGCAGGCGTCCACGGAATTGCAGTTGAGGTTTGGTGGGGAATCGTAGAGCGTTTCTCTCCTCTTGCATATGATTGGTCTCTATACGAAGAGCTTTTCAAACTGATTTCTGAGTCAGGTTTGAAGTTGCATGTTGCCTTGTCTTTTCATTCAAATGTTAACTCTTCTTCTAGCAGAAAAGGAGGTGTAAGTCTTCCGCTATGGATCGTAGAGATCGGTGATCAGAATAAGCATATATATTATCGCGATCAAAATGGGTATTCCAATGATGATTATCTTACACTCGGAGTTGATCATGTTCCTCTCTTTTGTGGCCGGACTGCCCTCCAATGTTATGAAGATTTCATGTCAAATTTTGCTAAGAAATTTGAATCTTTTATCGGAACTGTGATAGAAGAAATTAGTGTTGGCCTTGGTCCTTCTGGTGAACTTAGATATCCTGCACATCCTTTTGGTGATGGTAGATGGAAGTTTCCTGGAATCGGTGAATTCCAATGCTATGACAAGTACATGATGGATGACCTAAAGATGGCTGCACGCAAGGAAGGAAAGCCTCAGTGGGGAGAGAGGGGACCACAAAATGCTGGCTGCTACAACAGTCTCCCATCTGAAGTTCCTTTCTTTGAAGAGGGAGAGGAAAGCTTTCTTTCTGATTATGGGTGTTTTTTCCTTGTATGA

Protein Analysis

347

Amino Acids

38.96

Weight (kDa)

7.98

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 90 - 345 1.1e-68 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 609
AccIII TCCGGA 1 cut(s) 114
AciI CCGC 3 cut(s) 11, 100, 560
AclI AACGTT 1 cut(s) 120
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 4 cut(s) 255, 715, 728, 851
AcuI CTGAAG 2 cut(s) 52, 993
AcvI CACGTG 1 cut(s) 209
AcyI GRCGYC 2 cut(s) 310, 372
AfaI GTAC 1 cut(s) 872
AfiI CCNNNNNNNGG 3 cut(s) 845, 892, 923
AgsI TTSAA 5 cut(s) 463, 487, 516, 734, 989
AjnI CCWGG 2 cut(s) 47, 838
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 5 cut(s) 57, 81, 347, 457, 1005
AluI AGCT 5 cut(s) 57, 81, 347, 457, 1005
Alw26I GTCTC 2 cut(s) 447, 968
AlwI GGATC 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 243
Aor13HI TCCGGA 1 cut(s) 114
AoxI GGCC 3 cut(s) 333, 676, 772
ApeKI GCWGC 5 cut(s) 44, 162, 165, 896, 951
ApoI RAATTY 4 cut(s) 255, 715, 728, 851
Asp700I GAANNNNTTC 1 cut(s) 1005
AspS9I GGNCC 3 cut(s) 222, 779, 935
AsuHPI GGTGA 4 cut(s) 593, 800, 830, 860
AvaI CYCGRG 1 cut(s) 243
AvaII GGWCC 3 cut(s) 222, 779, 935
BbrPI CACGTG 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 5 cut(s) 56, 149, 177, 883, 938
BccI CCATC 8 cut(s) 178, 244, 293, 815, 822, 871, 886, 976
BceAI ACGGC 3 cut(s) 57, 113, 163
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 2 cut(s) 49, 840
BclI TGATCA 2 cut(s) 583, 655
BcoDI GTCTC 2 cut(s) 447, 968
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 163, 342
BisI GCNGC 5 cut(s) 45, 163, 166, 897, 952
BlsI GCNGC 5 cut(s) 46, 164, 167, 898, 953
Bme1390I CCNGG 2 cut(s) 49, 840
Bme18I GGWCC 3 cut(s) 222, 779, 935
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 222, 779, 935
BmiI GGNNCC 1 cut(s) 936
BmrFI CCNGG 2 cut(s) 49, 840
BmsI GCATC 4 cut(s) 159, 193, 264, 274
BpiI GAAGAC 1 cut(s) 548
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpuEI CTTGAG 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 2 cut(s) 310, 372
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 4 cut(s) 218, 314, 376, 775
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 845, 892, 923
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 114
BseBI CCWGG 2 cut(s) 49, 840
BseDI CCNNGG 4 cut(s) 218, 314, 376, 775
BseGI GGATG 4 cut(s) 208, 239, 808, 886
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 3 cut(s) 845, 892, 923
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 208, 465, 932
BseRI GAGGAG 2 cut(s) 67, 414
BseXI GCAGC 5 cut(s) 56, 149, 177, 883, 938
BsgI GTGCAG 2 cut(s) 789, 882
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 3 cut(s) 335, 678, 774
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 3 cut(s) 115, 180, 679
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 3 cut(s) 845, 892, 923
BsmAI GTCTC 2 cut(s) 447, 968
BsmFI GGGAC 1 cut(s) 948
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 3 cut(s) 335, 678, 774
Bso31I GGTCTC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 243
Bsp13I TCCGGA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 567, 576, 583, 610, 655
Bsp68I TCGCGA 1 cut(s) 609
BspACI CCGC 3 cut(s) 11, 100, 560
BspANI GGCC 3 cut(s) 335, 678, 774
BspCNI CTCAG 3 cut(s) 207, 466, 931
BspEI TCCGGA 1 cut(s) 114
BspFNI CGCG 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 936
BspMAI CTGCAG 1 cut(s) 167
BspPI GGATC 1 cut(s) 575
BspQI GCTCTTC 1 cut(s) 447
BspTNI GGTCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 12
BssECI CCNNGG 4 cut(s) 218, 314, 376, 775
BssMI GATC 5 cut(s) 567, 576, 583, 610, 655
BssNI GRCGYC 2 cut(s) 310, 372
BssT1I CCWWGG 2 cut(s) 314, 775
Bst2UI CCWGG 2 cut(s) 49, 840
Bst4CI ACNGT 3 cut(s) 343, 751, 962
Bst6I CTCTTC 3 cut(s) 447, 532, 984
BstACI GRCGYC 2 cut(s) 310, 372
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 370, 901, 949
BstDEI CTNAG 5 cut(s) 194, 474, 723, 794, 918
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 4 cut(s) 208, 239, 808, 886
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 5 cut(s) 570, 579, 586, 613, 658
BstMAI GTCTC 2 cut(s) 447, 968
BstMBI GATC 5 cut(s) 567, 576, 583, 610, 655
BstMWI GCNNNNNNNGC 2 cut(s) 63, 353
BstNI CCWGG 2 cut(s) 49, 840
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 2 cut(s) 47, 838
BstSFI CTRYAG 2 cut(s) 163, 342
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 56, 149, 177, 883, 938
BstV2I GAAGAC 1 cut(s) 548
BsuRI GGCC 3 cut(s) 335, 678, 774
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 4 cut(s) 208, 239, 808, 886
BtsIMutI CAGTG 1 cut(s) 926
BtuMI TCGCGA 1 cut(s) 609
Cac8I GCNNGC 3 cut(s) 370, 901, 949
Cfr13I GGNCC 3 cut(s) 222, 779, 935
CseI GACGC 2 cut(s) 299, 361
Csp6I GTAC 1 cut(s) 871
CviAII CATG 4 cut(s) 494, 659, 709, 874
CviQI GTAC 1 cut(s) 871
DdeI CTNAG 5 cut(s) 194, 474, 723, 794, 918
DpnI GATC 5 cut(s) 569, 578, 585, 612, 657
DpnII GATC 5 cut(s) 567, 576, 583, 610, 655
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 447, 532, 984
EarI CTCTTC 3 cut(s) 447, 532, 984
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 314, 775
Eco147I AGGCCT 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 800
Eco47I GGWCC 3 cut(s) 222, 779, 935
Eco57I CTGAAG 2 cut(s) 52, 993
Eco72I CACGTG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 243
EcoO109I RGGNCCY 1 cut(s) 222
EcoRI GAATTC 2 cut(s) 255, 851
EcoRII CCWGG 2 cut(s) 47, 838
EcoRV GATATC 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 314, 775
ErhI CCWWGG 2 cut(s) 314, 775
FaeI CATG 4 cut(s) 497, 662, 712, 877
FaqI GGGAC 1 cut(s) 948
FatI CATG 4 cut(s) 493, 658, 708, 873
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 2 cut(s) 583, 655
Fnu4HI GCNGC 5 cut(s) 45, 163, 166, 897, 952
FokI GGATG 4 cut(s) 215, 226, 795, 893
Fsp4HI GCNGC 5 cut(s) 45, 163, 166, 897, 952
FspBI CTAG 1 cut(s) 534
GluI GCNGC 5 cut(s) 45, 163, 166, 897, 952
HaeIII GGCC 3 cut(s) 335, 678, 774
HapII CCGG 3 cut(s) 115, 180, 679
HgaI GACGC 2 cut(s) 299, 361
Hin1I GRCGYC 2 cut(s) 310, 372
Hin1II CATG 4 cut(s) 497, 662, 712, 877
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 5 cut(s) 214, 405, 476, 734, 843
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 3 cut(s) 115, 180, 679
HphI GGTGA 4 cut(s) 593, 800, 830, 860
Hpy166II GTNNAC 3 cut(s) 376, 523, 791
Hpy188I TCNGA 8 cut(s) 71, 219, 475, 588, 650, 746, 973, 1015
Hpy188III TCNNGA 3 cut(s) 115, 243, 608
Hpy8I GTNNAC 3 cut(s) 376, 523, 791
HpyAV CCTTC 2 cut(s) 792, 902
HpyCH4III ACNGT 3 cut(s) 343, 751, 962
HpyCH4IV ACGT 2 cut(s) 120, 208
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 353
HpyF3I CTNAG 5 cut(s) 194, 474, 723, 794, 918
HpySE526I ACGT 2 cut(s) 120, 208
Hsp92I GRCGYC 2 cut(s) 310, 372
Hsp92II CATG 4 cut(s) 497, 662, 712, 877
Kpn2I TCCGGA 1 cut(s) 114
Ksp22I TGATCA 2 cut(s) 583, 655
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 5 cut(s) 567, 576, 583, 610, 655
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 5 cut(s) 56, 149, 177, 883, 938
LweI GCATC 4 cut(s) 159, 193, 264, 274
MaeI CTAG 1 cut(s) 534
MaeII ACGT 2 cut(s) 120, 208
MalI GATC 5 cut(s) 569, 578, 585, 612, 657
MboI GATC 5 cut(s) 567, 576, 583, 610, 655
MboII GAAGA 9 cut(s) 52, 145, 464, 519, 522, 548, 713, 770, 1001
MluCI AATT 6 cut(s) 255, 381, 715, 728, 762, 851
MlyI GAGTC 1 cut(s) 485
MroI TCCGGA 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 4 cut(s) 141, 338, 351, 522
MslI CAYNNNNRTG 2 cut(s) 266, 516
MspI CCGG 3 cut(s) 115, 180, 679
MspR9I CCNGG 2 cut(s) 49, 840
Mva1269I GAATGC 1 cut(s) 189
MvaI CCWGG 2 cut(s) 49, 840
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 63, 353
NdeI CATATG 1 cut(s) 433
NdeII GATC 5 cut(s) 567, 576, 583, 610, 655
NlaIII CATG 4 cut(s) 497, 662, 712, 877
NlaIV GGNNCC 1 cut(s) 936
NruI TCGCGA 1 cut(s) 609
NspI RCATGY 1 cut(s) 497
PceI AGGCCT 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 4 cut(s) 214, 405, 734, 843
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 5 cut(s) 46, 164, 167, 898, 953
PleI GAGTC 1 cut(s) 484
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
PpsI GAGTC 1 cut(s) 484
Ppu21I YACGTR 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 47, 838
PspCI CACGTG 1 cut(s) 209
PspGI CCWGG 2 cut(s) 47, 838
PspN4I GGNNCC 1 cut(s) 936
PspPI GGNCC 3 cut(s) 222, 779, 935
PspPPI RGGWCCY 1 cut(s) 222
PstI CTGCAG 1 cut(s) 167
RruI TCGCGA 1 cut(s) 609
RsaI GTAC 1 cut(s) 872
RsaNI GTAC 1 cut(s) 871
RseI CAYNNNNRTG 2 cut(s) 266, 516
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 4 cut(s) 141, 338, 351, 522
SatI GCNGC 5 cut(s) 45, 163, 166, 897, 952
Sau3AI GATC 5 cut(s) 567, 576, 583, 610, 655
Sau96I GGNCC 3 cut(s) 222, 779, 935
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 49, 840
SfaNI GCATC 4 cut(s) 159, 193, 264, 274
SfcI CTRYAG 2 cut(s) 163, 342
SinI GGWCC 3 cut(s) 222, 779, 935
SmiMI CAYNNNNRTG 2 cut(s) 266, 516
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 6 cut(s) 255, 381, 715, 728, 762, 851
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 3 cut(s) 11, 100, 560
SspMI CTAG 1 cut(s) 534
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 47, 838
StyI CCWWGG 2 cut(s) 314, 775
TaaI ACNGT 3 cut(s) 343, 751, 962
TaiI ACGT 2 cut(s) 123, 211
TaqI TCGA 2 cut(s) 84, 227
TasI AATT 6 cut(s) 255, 381, 715, 728, 762, 851
TatI WGTACW 1 cut(s) 870
TfiI GAWTC 4 cut(s) 214, 405, 734, 843
Tru1I TTAA 4 cut(s) 141, 338, 351, 522
Tru9I TTAA 4 cut(s) 141, 338, 351, 522
TscAI CASTG 1 cut(s) 926
TseI GCWGC 5 cut(s) 44, 162, 165, 896, 951
TspDTI ATGAA 3 cut(s) 500, 697, 714
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 926
VpaK11BI GGWCC 3 cut(s) 222, 779, 935
XapI RAATTY 4 cut(s) 255, 715, 728, 851
XceI RCATGY 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.