Rw5G033280
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008239
Physical Location & Seq
Reverse (-)
56458456 .. 56462702
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033280.1

Sequence Viewer

Length: 1536 bp
ATGGCAATGGCTGAGAATGGTGGAGTAGTCTGCAAATGCACAGCCAGGAGGAGTTTCCGGTTCAGACAACCCAAGCACTCCAATCTCCGAAACCTTTCCAGAATTCCTCTCTTCACTCGCCGCCCATCTCTTGCCGGAAAACTCACCCTTCTCAGCACGAGTGCACAAGAGAAATCAAGGCCTGCGGTATTGTCATCTACGAGACATAAGAGAATTCCCATATATGTGATGATGCCTGTTGATGCATTCTGCATAGATGGTTTTGGGATTCCAAGGATTAGGAGACTCAAGGCCTTAACTGCATCTCTAAAAGCACTCAAGTTGGCAGGTGTCCATGGAATTGCAGTTGAGGTTTGGTGGGGAATCGTAGAACGTTTCTCTCCCCTTGCATATGACTGGTCTCTATATGAAAAGCTATTTGAGCTGATTTCTGAGTCAGGGTTAAAGGTGCATGTTGCCTTGTCTTTTCACTCAAATGTAAACTCTTCTTCTTGCAGAAATGGGGGTGTAAGTCTTCCGCTTTGGATCACACAGATTGGTGACAGCAATAAGCATATATACTATCGAGACAAAAATGGGTTTTCAAGTGATGATTATCTTACGCTAGGGGTTGATAACCTTCCTCTGTTTTGTGGCCGGACTGCCCTTCAATGTTATGAAGATTTTATGACCAGTTTTGTTAAGAAATTTGAATCCCATATTGGAAGTGTCATAGAAGAAATTTGTGTTGGTCTTGGTCCTTCTGGTGAACTTAGGTATCCTGCACATTTTGGTGATGGTATATGGAAGTTCCCTGGAATTGGCGAGTTCCAGTGCTATGACAAGTACATGCTGGACAACCTAAAGATGGCCGCATGTGAGGAAGGGAAGCCTCAATGGGGAGAAGGGGGACCACCAAATGCTGGCTATTACAATAGTCTTCCATCTGGAGCTCCTTTCTTTGAAGAGGGAGAGGAAAGCTTTCTTTCTGATTATGGGTGCTTTTTCCTTGAATGGTATAGTGGTAGTTTGCTTGGTCATGCAGATGCTATTCTTGCAAAGGCAGCCAAAATTTTGAAGAAGTATCAAGAAGACAAGCAAACTTCTATTCTGTTAGTGGCTAAAATTGGTGGAATATATTGGTGGTACCAGACAGTAGCACACCCTGCTGAACTCACAGCTGGATACTACAACACTGCTCTGAGGGATGGTTATGATCCTGTTGCTTCAATTTTTTCTCGTCATGGAGCTGCTTTGCATGTGTCCTGCTTGGAAATGATGGATGGTGAAACACCAGAATCCTATCTCTGCAGCCCAGAAGGATTACTGCAACAGTTATGGAGTGTTTCGAAGAAAAGGATAATACATTTGATCGGGAGAAACACCAACGAACGATTTGACAGGGTTAGTTTATGGCAAATACATGACAACTGTTACCATTCACAGGCGGAAGTTGTAAGATCATTTACTTATTTCAGGATGAATGATAAGATCTTTAGGGCTGAAAACTGGAGTAACTTCGTTCCTTTTGTTAAAAAAATGAGCACAAGTTGGTGA

Protein Analysis

511

Amino Acids

57.73

Weight (kDa)

8.54

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 76 - 503 3e-106 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
Acc65I GGTACC 1 cut(s) 1125
AccB1I GGYRCC 1 cut(s) 1125
AccB7I CCANNNNNTGG 1 cut(s) 902
AciI CCGC 5 cut(s) 121, 185, 518, 852, 1427
AclI AACGTT 1 cut(s) 373
AclWI GGATC 2 cut(s) 533, 1190
AcoI YGGCCR 2 cut(s) 634, 849
AcsI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
AfaI GTAC 2 cut(s) 827, 1127
AfiI CCNNNNNNNGG 5 cut(s) 800, 847, 878, 902, 1423
AgsI TTSAA 7 cut(s) 585, 650, 692, 944, 992, 1057, 1209
AjnI CCWGG 2 cut(s) 44, 793
AjuI GAANNNNNNNTTGG 4 cut(s) 684, 711, 716, 743
AluBI AGCT 6 cut(s) 415, 424, 932, 960, 1160, 1229
AluI AGCT 6 cut(s) 415, 424, 932, 960, 1160, 1229
Alw21I GWGCWC 3 cut(s) 166, 934, 1526
Alw26I GTCTC 4 cut(s) 196, 277, 405, 561
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 2 cut(s) 533, 1190
AoxI GGCC 4 cut(s) 179, 291, 634, 849
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 1043, 1229, 1290
ApoI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
Asp700I GAANNNNTTC 2 cut(s) 94, 960
Asp718I GGTACC 1 cut(s) 1125
AspS9I GGNCC 2 cut(s) 737, 890
AsuHPI GGTGA 5 cut(s) 136, 551, 758, 785, 1277
AsuII TTCGAA 1 cut(s) 1328
AvaII GGWCC 2 cut(s) 737, 890
BaeGI GKGCMC 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 740, 773
BanI GGYRCC 1 cut(s) 1125
BanII GRGCYC 1 cut(s) 934
BauI CACGAG 1 cut(s) 157
BbsI GAAGAC 3 cut(s) 506, 911, 1077
Bbv12I GWGCWC 3 cut(s) 166, 934, 1526
BbvI GCAGC 3 cut(s) 1055, 1216, 1302
BccI CCATC 8 cut(s) 133, 251, 770, 841, 931, 1181, 1252, 1256
BciT130I CCWGG 2 cut(s) 46, 795
BciVI GTATCC 2 cut(s) 768, 1157
BcoDI GTCTC 4 cut(s) 196, 277, 405, 561
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 1288
BfuAI ACCTGC 1 cut(s) 317
BfuI GTATCC 2 cut(s) 768, 1157
BglII AGATCT 1 cut(s) 1470
BisI GCNGC 5 cut(s) 121, 852, 1044, 1230, 1291
BlsI GCNGC 5 cut(s) 122, 853, 1045, 1231, 1292
Bme1390I CCNGG 2 cut(s) 46, 795
Bme18I GGWCC 2 cut(s) 737, 890
BmgT120I GGNCC 2 cut(s) 737, 890
BmiI GGNNCC 2 cut(s) 891, 1127
BmrFI CCNGG 2 cut(s) 46, 795
BmsI GCATC 4 cut(s) 222, 232, 311, 1015
BpiI GAAGAC 3 cut(s) 506, 911, 1077
BpmI CTGGAG 2 cut(s) 948, 1510
Bpu14I TTCGAA 1 cut(s) 1328
BpuEI CTTGAG 2 cut(s) 272, 302
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 272, 334, 793
BsaWI WCCGGW 1 cut(s) 57
Bsc4I CCNNNNNNNGG 5 cut(s) 800, 847, 878, 902, 1423
Bse1I ACTGG 4 cut(s) 401, 672, 811, 1493
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 2 cut(s) 46, 795
BseDI CCNNGG 3 cut(s) 272, 334, 793
BseGI GGATG 3 cut(s) 1192, 1267, 1464
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 5 cut(s) 800, 847, 878, 902, 1423
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 166, 423, 1172
BseNI ACTGG 4 cut(s) 401, 672, 811, 1493
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 3 cut(s) 1055, 1216, 1302
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 4 cut(s) 181, 293, 636, 851
BshNI GGYRCC 1 cut(s) 1125
BsiHKAI GWGCWC 3 cut(s) 166, 934, 1526
BsiSI CCGG 3 cut(s) 58, 135, 637
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 5 cut(s) 800, 847, 878, 902, 1423
BsmAI GTCTC 4 cut(s) 196, 277, 405, 561
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 4 cut(s) 181, 293, 636, 851
Bso31I GGTCTC 1 cut(s) 405
Bsp119I TTCGAA 1 cut(s) 1328
Bsp1286I GDGCHC 3 cut(s) 166, 934, 1526
Bsp143I GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 5 cut(s) 121, 185, 518, 852, 1427
BspANI GGCC 4 cut(s) 181, 293, 636, 851
BspCNI CTCAG 4 cut(s) 4, 165, 424, 1173
BspLI GGNNCC 2 cut(s) 891, 1127
BspMAI CTGCAG 1 cut(s) 1292
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 533, 1190
BspT104I TTCGAA 1 cut(s) 1328
BspT107I GGYRCC 1 cut(s) 1125
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 4 cut(s) 401, 672, 811, 1493
BssECI CCNNGG 3 cut(s) 272, 334, 793
BssMI GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
BssSI CACGAG 1 cut(s) 157
BssT1I CCWWGG 2 cut(s) 272, 334
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 46, 795
Bst4CI ACNGT 3 cut(s) 1135, 1314, 1412
Bst6I CTCTTC 3 cut(s) 116, 490, 939
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstBI TTCGAA 1 cut(s) 1328
BstC8I GCNNGC 2 cut(s) 183, 904
BstDEI CTNAG 5 cut(s) 12, 152, 432, 752, 1181
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 3 cut(s) 1192, 1267, 1464
BstKTI GATC 5 cut(s) 528, 1198, 1353, 1442, 1473
BstMAI GTCTC 4 cut(s) 196, 277, 405, 561
BstMBI GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
BstMWI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
BstNI CCWGG 2 cut(s) 46, 795
BstNSI RCATGY 4 cut(s) 455, 832, 858, 1241
BstSCI CCNGG 2 cut(s) 44, 793
BstSFI CTRYAG 1 cut(s) 1288
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 3 cut(s) 1055, 1216, 1302
BstV2I GAAGAC 3 cut(s) 506, 911, 1077
BstX2I RGATCY 1 cut(s) 1470
BstYI RGATCY 1 cut(s) 1470
BsuI GTATCC 2 cut(s) 768, 1157
BsuRI GGCC 4 cut(s) 181, 293, 636, 851
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 3 cut(s) 1192, 1267, 1464
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 2 cut(s) 818, 1173
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 183, 904
Cfr13I GGNCC 2 cut(s) 737, 890
Csp6I GTAC 2 cut(s) 826, 1126
CviAII CATG 8 cut(s) 335, 452, 829, 855, 1019, 1223, 1238, 1403
CviQI GTAC 2 cut(s) 826, 1126
DdeI CTNAG 5 cut(s) 12, 152, 432, 752, 1181
DpnI GATC 5 cut(s) 527, 1197, 1352, 1441, 1472
DpnII GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
EaeI YGGCCR 2 cut(s) 634, 849
Eam1104I CTCTTC 3 cut(s) 116, 490, 939
EarI CTCTTC 3 cut(s) 116, 490, 939
EciI GGCGGA 1 cut(s) 1442
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 272, 334
Eco147I AGGCCT 2 cut(s) 181, 293
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 737, 890
Eco53kI GAGCTC 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 932
EcoRI GAATTC 2 cut(s) 102, 213
EcoRII CCWGG 2 cut(s) 44, 793
EcoT14I CCWWGG 2 cut(s) 272, 334
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 2 cut(s) 272, 334
FaeI CATG 8 cut(s) 338, 455, 832, 858, 1022, 1226, 1241, 1406
FaqI GGGAC 1 cut(s) 903
FatI CATG 8 cut(s) 334, 451, 828, 854, 1018, 1222, 1237, 1402
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 5 cut(s) 121, 852, 1044, 1230, 1291
FokI GGATG 3 cut(s) 1199, 1274, 1471
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 5 cut(s) 121, 852, 1044, 1230, 1291
FspBI CTAG 1 cut(s) 605
GluI GCNGC 5 cut(s) 121, 852, 1044, 1230, 1291
GsuI CTGGAG 2 cut(s) 948, 1510
HaeIII GGCC 4 cut(s) 181, 293, 636, 851
HapII CCGG 3 cut(s) 58, 135, 637
Hin1II CATG 8 cut(s) 338, 455, 832, 858, 1022, 1226, 1241, 1406
HindIII AAGCTT 1 cut(s) 958
HinfI GANTC 6 cut(s) 268, 285, 363, 434, 692, 1277
HpaII CCGG 3 cut(s) 58, 135, 637
HphI GGTGA 5 cut(s) 136, 551, 758, 785, 1277
Hpy166II GTNNAC 3 cut(s) 164, 481, 749
Hpy188I TCNGA 5 cut(s) 65, 89, 433, 970, 1182
Hpy188III TCNNGA 6 cut(s) 99, 566, 927, 1067, 1354, 1456
Hpy8I GTNNAC 3 cut(s) 164, 481, 749
HpyAV CCTTC 7 cut(s) 158, 629, 656, 750, 857, 878, 1292
HpyCH4III ACNGT 3 cut(s) 1135, 1314, 1412
HpyCH4IV ACGT 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
HpyF3I CTNAG 5 cut(s) 12, 152, 432, 752, 1181
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 8 cut(s) 338, 455, 832, 858, 1022, 1226, 1241, 1406
KpnI GGTACC 1 cut(s) 1129
Kzo9I GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
LmnI GCTCC 3 cut(s) 929, 937, 1226
Lsp1109I GCAGC 3 cut(s) 1055, 1216, 1302
LweI GCATC 4 cut(s) 222, 232, 311, 1015
MaeI CTAG 1 cut(s) 605
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 3 cut(s) 539, 1412, 1493
MalI GATC 5 cut(s) 527, 1197, 1352, 1441, 1472
MboI GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
MflI RGATCY 1 cut(s) 1470
MhlI GDGCHC 3 cut(s) 166, 934, 1526
MluCI AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
MlyI GAGTC 2 cut(s) 279, 443
MnlI CCTC 9 cut(s) 42, 117, 343, 633, 853, 882, 940, 946, 1176
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 94, 960
MseI TTAA 4 cut(s) 296, 443, 681, 1512
MslI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 3 cut(s) 58, 135, 637
MspR9I CCNGG 2 cut(s) 46, 795
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 2 cut(s) 46, 795
MwoI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 1 cut(s) 391
NdeII GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
NlaIII CATG 8 cut(s) 338, 455, 832, 858, 1022, 1226, 1241, 1406
NlaIV GGNNCC 2 cut(s) 891, 1127
NmuCI GTSAC 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 4 cut(s) 455, 832, 858, 1241
NspV TTCGAA 1 cut(s) 1328
PaqCI CACCTGC 1 cut(s) 317
PceI AGGCCT 2 cut(s) 181, 293
PctI GAATGC 1 cut(s) 245
PdmI GAANNNNTTC 2 cut(s) 94, 960
PfeI GAWTC 4 cut(s) 268, 363, 692, 1277
PflMI CCANNNNNTGG 1 cut(s) 902
PkrI GCNGC 5 cut(s) 122, 853, 1045, 1231, 1292
PleI GAGTC 2 cut(s) 279, 442
PpsI GAGTC 2 cut(s) 279, 442
Psp124BI GAGCTC 1 cut(s) 934
Psp1406I AACGTT 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 44, 793
PspGI CCWGG 2 cut(s) 44, 793
PspN4I GGNNCC 2 cut(s) 891, 1127
PspPI GGNCC 2 cut(s) 737, 890
PstI CTGCAG 1 cut(s) 1292
PsuI RGATCY 1 cut(s) 1470
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 2 cut(s) 827, 1127
RsaNI GTAC 2 cut(s) 826, 1126
RseI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
SacI GAGCTC 1 cut(s) 934
SaqAI TTAA 4 cut(s) 296, 443, 681, 1512
SatI GCNGC 5 cut(s) 121, 852, 1044, 1230, 1291
Sau3AI GATC 5 cut(s) 525, 1195, 1350, 1439, 1470
Sau96I GGNCC 2 cut(s) 737, 890
SchI GAGTC 2 cut(s) 279, 443
ScrFI CCNGG 2 cut(s) 46, 795
SduI GDGCHC 3 cut(s) 166, 934, 1526
SfaNI GCATC 4 cut(s) 222, 232, 311, 1015
SfcI CTRYAG 1 cut(s) 1288
SfuI TTCGAA 1 cut(s) 1328
SinI GGWCC 2 cut(s) 737, 890
SmiMI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
SmlI CTYRAG 2 cut(s) 287, 317
SmoI CTYRAG 2 cut(s) 287, 317
Sse9I AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
SseBI AGGCCT 2 cut(s) 181, 293
SsiI CCGC 5 cut(s) 121, 185, 518, 852, 1427
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 1 cut(s) 934
StuI AGGCCT 2 cut(s) 181, 293
StyD4I CCNGG 2 cut(s) 44, 793
StyI CCWWGG 2 cut(s) 272, 334
TaaI ACNGT 3 cut(s) 1135, 1314, 1412
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 565, 1328
TasI AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
TatI WGTACW 1 cut(s) 825
TauI GCSGC 2 cut(s) 123, 854
TfiI GAWTC 4 cut(s) 268, 363, 692, 1277
Tru1I TTAA 4 cut(s) 296, 443, 681, 1512
Tru9I TTAA 4 cut(s) 296, 443, 681, 1512
TscAI CASTG 2 cut(s) 818, 1180
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 3 cut(s) 1043, 1229, 1290
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 3 cut(s) 423, 672, 1475
TspRI CASTG 2 cut(s) 818, 1180
Van91I CCANNNNNTGG 1 cut(s) 902
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 737, 890
XapI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
XceI RCATGY 4 cut(s) 455, 832, 858, 1241
XmnI GAANNNNTTC 2 cut(s) 94, 960
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.