Rh5CG388600
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008239
Physical Location & Seq
Reverse (-)
51151925 .. 51154754
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG388600.1

Sequence Viewer

Length: 1248 bp
ATGGCAATGGCTGAGAATGGTGGAGTGGTCTGCAAATGCACAGCCAGGAGGAGTTTCCGCTTCAGACAACCCAAGCACTCCAATCTCCGAAACCTTTCCAGAATTCCTCTCTTCACTCGCCGCCCATCTCTTGCCGGAAAACTTACCCTTCTCAGCACGAGTGCACAAGAGAAATCGAGGCCTGCGGTATTGTCATCTATGAGACATGAGAGAATTCCCATATATGTGATGATGCCTGTTGATGCATTCTGCATAGATGGTTCTGGGATTCCAAGGATTAGGAGACTCAAGGCCTTAACTGCATCTCTAAAAGCACTCAAGTTGGCAGGTGTCCATGGAATTGCAGTCGAGGTTTGGTGGGGAATCGTAGAACATTTCTCTCCCCTTGCATATGACTGGTCTCTATATGAAAAGCTATTTAAGCTGATTTCTGAGTCAGGGTTAAAGGTGCATGTTGCCTTGTCTTTTCACTCAAATGTAAACTCTTCTTCTTGCACAAATGGGGGTGTTAGTCTTCCGCTTTGGATCACACAGATTGGTGACAGCAATAAGCATATATACTATCGAGACAAAAATGGGTTTTCAAGTGATGATTATCTTACACTAGGGGTTGATAACCTTCCTCTGTTTTGTGGCCGGACTGCCCTTCAATGTTATGAAGATTTTATGACCAGTTTTGTTAAGAAATTTGAATCCCATATTGGAAGTGTCATAGAAGAAATTTGTGTTGGTCTTGGTCCTTCTGGTGAACTTAGGTATCCTGCACATTTTGGTGATGGTATATGGAAGTTCCCTGGAATTGGCGAGTTCCAGTGCTATGACAAGTACATGCTGGACAACCTAAAGATGGCCGCATGTGAGGAAGGGAAGCCTCAATGGGGAGAAGGGGGACCACCAAATGCTGGCTATTACAATAGTCTTCCATCTGGAGCTCCTTTCTTTGAAGAGGGAGAGGAAAGCTTTCTTTCTGATTATGGGTGCTTTTTCCTTGAATGGTATAGTGGTAGTTTGCTTGGTCATGCAGATGCTATTCTTGCAAAGGCAGCCAAAATTTTGAAGAAGTATCAAGAAGACAAGCAAACTTCTATTCTGTTAGTGGCTAAAATTGGTGGAATATATTGGTGGTACCAGACAGTAGCACACCCTGCTGAACTCACAGCTGGATACTACAACACTGCTCTTAGGGATGGTTATGATCCTGTTGCTTCAATTTTTTCTCGTCATGGAGCTGCTTTGCATGTGTCGTAA

Protein Analysis

415

Amino Acids

46.14

Weight (kDa)

8.22

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 76 - 413 4.3e-87 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
Acc65I GGTACC 1 cut(s) 1125
AccB1I GGYRCC 1 cut(s) 1125
AccB7I CCANNNNNTGG 1 cut(s) 902
AciI CCGC 5 cut(s) 58, 121, 185, 518, 852
AclWI GGATC 2 cut(s) 533, 1190
AcoI YGGCCR 2 cut(s) 634, 849
AcsI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
AcuI CTGAAG 1 cut(s) 46
AfaI GTAC 2 cut(s) 827, 1127
AfiI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
AgsI TTSAA 7 cut(s) 585, 650, 692, 944, 992, 1057, 1209
AjnI CCWGG 2 cut(s) 44, 793
AjuI GAANNNNNNNTTGG 4 cut(s) 684, 711, 716, 743
AluBI AGCT 6 cut(s) 415, 424, 932, 960, 1160, 1229
AluI AGCT 6 cut(s) 415, 424, 932, 960, 1160, 1229
Alw21I GWGCWC 2 cut(s) 166, 934
Alw26I GTCTC 4 cut(s) 196, 277, 405, 561
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 2 cut(s) 533, 1190
AoxI GGCC 4 cut(s) 179, 291, 634, 849
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 1043, 1229
ApoI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
Asp700I GAANNNNTTC 2 cut(s) 94, 960
Asp718I GGTACC 1 cut(s) 1125
AspS9I GGNCC 2 cut(s) 737, 890
AsuHPI GGTGA 3 cut(s) 551, 758, 785
AvaII GGWCC 2 cut(s) 737, 890
BaeGI GKGCMC 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 740, 773
BanI GGYRCC 1 cut(s) 1125
BanII GRGCYC 1 cut(s) 934
BauI CACGAG 1 cut(s) 157
BbsI GAAGAC 3 cut(s) 506, 911, 1077
Bbv12I GWGCWC 2 cut(s) 166, 934
BbvI GCAGC 2 cut(s) 1055, 1216
BccI CCATC 6 cut(s) 133, 251, 770, 841, 931, 1181
BciT130I CCWGG 2 cut(s) 46, 795
BciVI GTATCC 2 cut(s) 768, 1157
BcoDI GTCTC 4 cut(s) 196, 277, 405, 561
BfaI CTAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 317
BfuI GTATCC 2 cut(s) 768, 1157
BisI GCNGC 4 cut(s) 121, 852, 1044, 1230
BlsI GCNGC 4 cut(s) 122, 853, 1045, 1231
Bme1390I CCNGG 2 cut(s) 46, 795
Bme18I GGWCC 2 cut(s) 737, 890
BmgT120I GGNCC 2 cut(s) 737, 890
BmiI GGNNCC 2 cut(s) 891, 1127
BmrFI CCNGG 2 cut(s) 46, 795
BmsI GCATC 4 cut(s) 222, 232, 311, 1015
BpiI GAAGAC 3 cut(s) 506, 911, 1077
BpmI CTGGAG 1 cut(s) 948
BpuEI CTTGAG 2 cut(s) 272, 302
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 272, 334, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
Bse1I ACTGG 3 cut(s) 401, 672, 811
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 2 cut(s) 46, 795
BseDI CCNNGG 3 cut(s) 272, 334, 793
BseGI GGATG 1 cut(s) 1192
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 166, 423
BseNI ACTGG 3 cut(s) 401, 672, 811
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 1055, 1216
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 4 cut(s) 181, 293, 636, 851
BshNI GGYRCC 1 cut(s) 1125
BsiHKAI GWGCWC 2 cut(s) 166, 934
BsiSI CCGG 2 cut(s) 135, 637
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
BsmAI GTCTC 4 cut(s) 196, 277, 405, 561
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 4 cut(s) 181, 293, 636, 851
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 166, 934
Bsp143I GATC 2 cut(s) 525, 1195
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 5 cut(s) 58, 121, 185, 518, 852
BspANI GGCC 4 cut(s) 181, 293, 636, 851
BspCNI CTCAG 3 cut(s) 4, 165, 424
BspLI GGNNCC 2 cut(s) 891, 1127
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 533, 1190
BspT107I GGYRCC 1 cut(s) 1125
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 3 cut(s) 401, 672, 811
BssECI CCNNGG 3 cut(s) 272, 334, 793
BssMI GATC 2 cut(s) 525, 1195
BssSI CACGAG 1 cut(s) 157
BssT1I CCWWGG 2 cut(s) 272, 334
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 46, 795
Bst4CI ACNGT 1 cut(s) 1135
Bst6I CTCTTC 3 cut(s) 116, 490, 939
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstC8I GCNNGC 2 cut(s) 183, 904
BstDEI CTNAG 5 cut(s) 12, 152, 432, 752, 1181
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 1192
BstKTI GATC 2 cut(s) 528, 1198
BstMAI GTCTC 4 cut(s) 196, 277, 405, 561
BstMBI GATC 2 cut(s) 525, 1195
BstMWI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
BstNI CCWGG 2 cut(s) 46, 795
BstNSI RCATGY 4 cut(s) 455, 832, 858, 1241
BstSCI CCNGG 2 cut(s) 44, 793
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 1055, 1216
BstV2I GAAGAC 3 cut(s) 506, 911, 1077
BsuI GTATCC 2 cut(s) 768, 1157
BsuRI GGCC 4 cut(s) 181, 293, 636, 851
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 1 cut(s) 1192
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 2 cut(s) 818, 1173
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 183, 904
Cfr13I GGNCC 2 cut(s) 737, 890
Csp6I GTAC 2 cut(s) 826, 1126
CviAII CATG 8 cut(s) 206, 335, 452, 829, 855, 1019, 1223, 1238
CviQI GTAC 2 cut(s) 826, 1126
DdeI CTNAG 5 cut(s) 12, 152, 432, 752, 1181
DpnI GATC 2 cut(s) 527, 1197
DpnII GATC 2 cut(s) 525, 1195
EaeI YGGCCR 2 cut(s) 634, 849
Eam1104I CTCTTC 3 cut(s) 116, 490, 939
EarI CTCTTC 3 cut(s) 116, 490, 939
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 272, 334
Eco147I AGGCCT 2 cut(s) 181, 293
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 737, 890
Eco53kI GAGCTC 1 cut(s) 932
Eco57I CTGAAG 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 932
EcoRI GAATTC 2 cut(s) 102, 213
EcoRII CCWGG 2 cut(s) 44, 793
EcoT14I CCWWGG 2 cut(s) 272, 334
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 2 cut(s) 272, 334
FaeI CATG 8 cut(s) 209, 338, 455, 832, 858, 1022, 1226, 1241
FaqI GGGAC 1 cut(s) 903
FatI CATG 8 cut(s) 205, 334, 451, 828, 854, 1018, 1222, 1237
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 121, 852, 1044, 1230
FokI GGATG 1 cut(s) 1199
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 4 cut(s) 121, 852, 1044, 1230
FspBI CTAG 1 cut(s) 605
GluI GCNGC 4 cut(s) 121, 852, 1044, 1230
GsuI CTGGAG 1 cut(s) 948
HaeIII GGCC 4 cut(s) 181, 293, 636, 851
HapII CCGG 2 cut(s) 135, 637
Hin1II CATG 8 cut(s) 209, 338, 455, 832, 858, 1022, 1226, 1241
HindIII AAGCTT 1 cut(s) 958
HinfI GANTC 5 cut(s) 268, 285, 363, 434, 692
HpaII CCGG 2 cut(s) 135, 637
HphI GGTGA 3 cut(s) 551, 758, 785
Hpy166II GTNNAC 3 cut(s) 164, 481, 749
Hpy188I TCNGA 4 cut(s) 65, 89, 433, 970
Hpy188III TCNNGA 4 cut(s) 99, 566, 927, 1067
Hpy8I GTNNAC 3 cut(s) 164, 481, 749
HpyAV CCTTC 6 cut(s) 158, 629, 656, 750, 857, 878
HpyCH4III ACNGT 1 cut(s) 1135
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
HpyF3I CTNAG 5 cut(s) 12, 152, 432, 752, 1181
Hsp92II CATG 8 cut(s) 209, 338, 455, 832, 858, 1022, 1226, 1241
KpnI GGTACC 1 cut(s) 1129
Kzo9I GATC 2 cut(s) 525, 1195
LmnI GCTCC 3 cut(s) 929, 937, 1226
Lsp1109I GCAGC 2 cut(s) 1055, 1216
LweI GCATC 4 cut(s) 222, 232, 311, 1015
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 539
MalI GATC 2 cut(s) 527, 1197
MboI GATC 2 cut(s) 525, 1195
MhlI GDGCHC 2 cut(s) 166, 934
MluCI AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
MlyI GAGTC 2 cut(s) 279, 443
MnlI CCTC 9 cut(s) 42, 117, 171, 343, 633, 853, 882, 940, 946
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 94, 960
MseI TTAA 4 cut(s) 296, 420, 443, 681
MslI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 2 cut(s) 135, 637
MspR9I CCNGG 2 cut(s) 46, 795
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 2 cut(s) 46, 795
MwoI GCNNNNNNNGC 5 cut(s) 299, 421, 1034, 1043, 1145
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 1 cut(s) 391
NdeII GATC 2 cut(s) 525, 1195
NlaIII CATG 8 cut(s) 209, 338, 455, 832, 858, 1022, 1226, 1241
NlaIV GGNNCC 2 cut(s) 891, 1127
NmuCI GTSAC 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 4 cut(s) 455, 832, 858, 1241
PaqCI CACCTGC 1 cut(s) 317
PceI AGGCCT 2 cut(s) 181, 293
PctI GAATGC 1 cut(s) 245
PdmI GAANNNNTTC 2 cut(s) 94, 960
PfeI GAWTC 3 cut(s) 268, 363, 692
PflMI CCANNNNNTGG 1 cut(s) 902
PkrI GCNGC 4 cut(s) 122, 853, 1045, 1231
PleI GAGTC 2 cut(s) 279, 442
PpsI GAGTC 2 cut(s) 279, 442
Psp124BI GAGCTC 1 cut(s) 934
Psp6I CCWGG 2 cut(s) 44, 793
PspGI CCWGG 2 cut(s) 44, 793
PspN4I GGNNCC 2 cut(s) 891, 1127
PspPI GGNCC 2 cut(s) 737, 890
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 2 cut(s) 827, 1127
RsaNI GTAC 2 cut(s) 826, 1126
RseI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
SacI GAGCTC 1 cut(s) 934
SaqAI TTAA 4 cut(s) 296, 420, 443, 681
SatI GCNGC 4 cut(s) 121, 852, 1044, 1230
Sau3AI GATC 2 cut(s) 525, 1195
Sau96I GGNCC 2 cut(s) 737, 890
SchI GAGTC 2 cut(s) 279, 443
ScrFI CCNGG 2 cut(s) 46, 795
SduI GDGCHC 2 cut(s) 166, 934
SfaNI GCATC 4 cut(s) 222, 232, 311, 1015
SinI GGWCC 2 cut(s) 737, 890
SmiMI CAYNNNNRTG 4 cut(s) 224, 474, 771, 1023
SmlI CTYRAG 2 cut(s) 287, 317
SmoI CTYRAG 2 cut(s) 287, 317
Sse9I AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
SseBI AGGCCT 2 cut(s) 181, 293
SsiI CCGC 5 cut(s) 58, 121, 185, 518, 852
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 1 cut(s) 934
StuI AGGCCT 2 cut(s) 181, 293
StyD4I CCNGG 2 cut(s) 44, 793
StyI CCWWGG 2 cut(s) 272, 334
TaaI ACNGT 1 cut(s) 1135
TaqI TCGA 3 cut(s) 176, 348, 565
TasI AATT 9 cut(s) 102, 213, 339, 686, 720, 798, 1050, 1104, 1209
TatI WGTACW 1 cut(s) 825
TauI GCSGC 2 cut(s) 123, 854
TfiI GAWTC 3 cut(s) 268, 363, 692
Tru1I TTAA 4 cut(s) 296, 420, 443, 681
Tru9I TTAA 4 cut(s) 296, 420, 443, 681
TscAI CASTG 2 cut(s) 818, 1180
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 2 cut(s) 1043, 1229
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 423, 672
TspRI CASTG 2 cut(s) 818, 1180
Van91I CCANNNNNTGG 1 cut(s) 902
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 737, 890
XapI RAATTY 5 cut(s) 102, 213, 686, 720, 1050
XceI RCATGY 4 cut(s) 455, 832, 858, 1241
XmnI GAANNNNTTC 2 cut(s) 94, 960
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.