RchiOBHm_Chr5g0053651
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008239
Physical Location & Seq
Reverse (-)
56034294 .. 56036702
2409 bp
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UTR
Exon/CDS
Intron
PRQ33093

Sequence Viewer

Length: 996 bp
ATGGCAATGGCTGAGAATGCTGGAGTAGTCTGCAAATGCACAGCCAGGAGGAGTTTCCGCTTCAGGCAACCCAAGCACTCCAATCTCCGAAACCTTTCCAGAATTCCTCTCTTCACTCGCCGCCCATCTCTTGCCGGAAAACTCACCCTTCTCAGCACGAGTGCACAAGAGAAATCAAGGCCTGCGGTATTGTCATCTACGAGACATAAGAGAATTCCCATATATGTGATGATGCCTGTTGATGCATTCTGCATAGATGGTTCTGGGATTCCAAGGATTAGGAGACTCAAGGCCTTAACTGCATCTCTAAAAGCACTCAAGTTGGCAGGTGTCCATGGAATTGCAGTTGAGGTTTGGTGGGGAATCATAGAACGTTTCTCTCCCCTTGCATATGACTGGTCTCTATATGAAAAGCTATTTGAGCTGATTTCTGAGTCAGGGTTAAAGGTGCATGTTGCCTTGTCTTTTCACTCAAATGTAAACTCTTCTTCTTGCAGAAATGGGGGTGTAAGTCTTCCGCTTTGGATCACACAGATTGGTGACAGCAATAAGCATATATACTATCGAGACAAAAATGGGTTTTCAAGTGATGATTATCTTACGCTAGGGGTTGATAACCTTCCTCTGTTTTGTGGCCGGACTGCCCTTCAATGTTATGAAGATTTTATGACCAGTTTTGTTAAGAAATTTGAATCCCATATTGGAAGTGTCATAGAAGAAATTTGTGTTGGTCTTGGTCCTTCTGGTGAACTTAGGTATCCTGCACATTTTGGTGATGGTATATGGAAGTTCCCTGGAATTGGCGAGTTCCAGTGCTATGACAAGTACATGCTGGACAACCTAAAGATGGCCGCATGTGAGGAAGGGAAGCCTCAATGGGGAGAAGGGGGACCACCAAATGCTGGCTATTACAATAGTCTTCCATCTGGAGCTCCTTTCTTTGAAGAGGGAGAGGAAAGCTTTCTTTCTGATTATGGGTGCTTTTTCCTTGTATGA

Protein Analysis

331

Amino Acids

36.94

Weight (kDa)

8.45

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 76 - 330 1.6e-63 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 902
AciI CCGC 5 cut(s) 58, 121, 185, 518, 852
AclI AACGTT 1 cut(s) 373
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 2 cut(s) 634, 849
AcsI RAATTY 4 cut(s) 102, 213, 686, 720
AcuI CTGAAG 1 cut(s) 46
AfaI GTAC 1 cut(s) 827
AfiI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
AgsI TTSAA 4 cut(s) 585, 650, 692, 944
AjnI CCWGG 2 cut(s) 44, 793
AjuI GAANNNNNNNTTGG 4 cut(s) 684, 711, 716, 743
AluBI AGCT 4 cut(s) 415, 424, 932, 960
AluI AGCT 4 cut(s) 415, 424, 932, 960
Alw21I GWGCWC 2 cut(s) 166, 934
Alw26I GTCTC 4 cut(s) 196, 277, 405, 561
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 1 cut(s) 533
AoxI GGCC 4 cut(s) 179, 291, 634, 849
ApaLI GTGCAC 1 cut(s) 162
ApoI RAATTY 4 cut(s) 102, 213, 686, 720
Asp700I GAANNNNTTC 2 cut(s) 94, 960
AspS9I GGNCC 2 cut(s) 737, 890
AsuHPI GGTGA 4 cut(s) 136, 551, 758, 785
AvaII GGWCC 2 cut(s) 737, 890
BaeGI GKGCMC 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 740, 773
BanII GRGCYC 1 cut(s) 934
BauI CACGAG 1 cut(s) 157
BbsI GAAGAC 2 cut(s) 506, 911
Bbv12I GWGCWC 2 cut(s) 166, 934
BccI CCATC 5 cut(s) 133, 251, 770, 841, 931
BciT130I CCWGG 2 cut(s) 46, 795
BciVI GTATCC 1 cut(s) 768
BcoDI GTCTC 4 cut(s) 196, 277, 405, 561
BfaI CTAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 317
BfuI GTATCC 1 cut(s) 768
BisI GCNGC 2 cut(s) 121, 852
BlsI GCNGC 2 cut(s) 122, 853
Bme1390I CCNGG 2 cut(s) 46, 795
Bme18I GGWCC 2 cut(s) 737, 890
BmgT120I GGNCC 2 cut(s) 737, 890
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 2 cut(s) 46, 795
BmsI GCATC 3 cut(s) 222, 232, 311
BpiI GAAGAC 2 cut(s) 506, 911
BpmI CTGGAG 2 cut(s) 42, 948
BpuEI CTTGAG 2 cut(s) 272, 302
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 272, 334, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
Bse1I ACTGG 3 cut(s) 401, 672, 811
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 2 cut(s) 46, 795
BseDI CCNNGG 3 cut(s) 272, 334, 793
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 166, 423
BseNI ACTGG 3 cut(s) 401, 672, 811
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 4 cut(s) 181, 293, 636, 851
BsiHKAI GWGCWC 2 cut(s) 166, 934
BsiSI CCGG 2 cut(s) 135, 637
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 4 cut(s) 800, 847, 878, 902
BsmAI GTCTC 4 cut(s) 196, 277, 405, 561
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 2 cut(s) 22, 245
BsnI GGCC 4 cut(s) 181, 293, 636, 851
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 166, 934
Bsp143I GATC 1 cut(s) 525
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 5 cut(s) 58, 121, 185, 518, 852
BspANI GGCC 4 cut(s) 181, 293, 636, 851
BspCNI CTCAG 3 cut(s) 4, 165, 424
BspLI GGNNCC 1 cut(s) 891
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 3 cut(s) 401, 672, 811
BssECI CCNNGG 3 cut(s) 272, 334, 793
BssMI GATC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 157
BssT1I CCWWGG 2 cut(s) 272, 334
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 46, 795
Bst6I CTCTTC 3 cut(s) 116, 490, 939
BstC8I GCNNGC 2 cut(s) 183, 904
BstDEI CTNAG 4 cut(s) 12, 152, 432, 752
BstDSI CCRYGG 1 cut(s) 334
BstKTI GATC 1 cut(s) 528
BstMAI GTCTC 4 cut(s) 196, 277, 405, 561
BstMBI GATC 1 cut(s) 525
BstMWI GCNNNNNNNGC 4 cut(s) 17, 73, 299, 421
BstNI CCWGG 2 cut(s) 46, 795
BstNSI RCATGY 3 cut(s) 455, 832, 858
BstSCI CCNGG 2 cut(s) 44, 793
BstSLI GKGCMC 1 cut(s) 166
BstV2I GAAGAC 2 cut(s) 506, 911
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 4 cut(s) 181, 293, 636, 851
BtgI CCRYGG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 818
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 183, 904
Cfr13I GGNCC 2 cut(s) 737, 890
Csp6I GTAC 1 cut(s) 826
CviAII CATG 4 cut(s) 335, 452, 829, 855
CviQI GTAC 1 cut(s) 826
DdeI CTNAG 4 cut(s) 12, 152, 432, 752
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 634, 849
Eam1104I CTCTTC 3 cut(s) 116, 490, 939
EarI CTCTTC 3 cut(s) 116, 490, 939
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 272, 334
Eco147I AGGCCT 2 cut(s) 181, 293
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 737, 890
Eco53kI GAGCTC 1 cut(s) 932
Eco57I CTGAAG 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 932
EcoRI GAATTC 2 cut(s) 102, 213
EcoRII CCWGG 2 cut(s) 44, 793
EcoT14I CCWWGG 2 cut(s) 272, 334
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 2 cut(s) 272, 334
FaeI CATG 4 cut(s) 338, 455, 832, 858
FaqI GGGAC 1 cut(s) 903
FatI CATG 4 cut(s) 334, 451, 828, 854
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 2 cut(s) 121, 852
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 2 cut(s) 121, 852
FspBI CTAG 1 cut(s) 605
GluI GCNGC 2 cut(s) 121, 852
GsuI CTGGAG 2 cut(s) 42, 948
HaeIII GGCC 4 cut(s) 181, 293, 636, 851
HapII CCGG 2 cut(s) 135, 637
Hin1II CATG 4 cut(s) 338, 455, 832, 858
HindIII AAGCTT 1 cut(s) 958
HinfI GANTC 5 cut(s) 268, 285, 363, 434, 692
HpaII CCGG 2 cut(s) 135, 637
HphI GGTGA 4 cut(s) 136, 551, 758, 785
Hpy166II GTNNAC 3 cut(s) 164, 481, 749
Hpy188I TCNGA 3 cut(s) 89, 433, 970
Hpy188III TCNNGA 3 cut(s) 99, 566, 927
Hpy8I GTNNAC 3 cut(s) 164, 481, 749
HpyAV CCTTC 6 cut(s) 158, 629, 656, 750, 857, 878
HpyCH4IV ACGT 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 73, 299, 421
HpyF3I CTNAG 4 cut(s) 12, 152, 432, 752
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 4 cut(s) 338, 455, 832, 858
Kzo9I GATC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 929, 937
LweI GCATC 3 cut(s) 222, 232, 311
MaeI CTAG 1 cut(s) 605
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 539
MalI GATC 1 cut(s) 527
MboI GATC 1 cut(s) 525
MboII GAAGA 8 cut(s) 103, 477, 480, 506, 671, 728, 911, 956
MhlI GDGCHC 2 cut(s) 166, 934
MluCI AATT 6 cut(s) 102, 213, 339, 686, 720, 798
MlyI GAGTC 2 cut(s) 279, 443
MnlI CCTC 8 cut(s) 42, 117, 343, 633, 853, 882, 940, 946
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 94, 960
MseI TTAA 3 cut(s) 296, 443, 681
MslI CAYNNNNRTG 3 cut(s) 224, 474, 771
MspI CCGG 2 cut(s) 135, 637
MspR9I CCNGG 2 cut(s) 46, 795
Mva1269I GAATGC 2 cut(s) 22, 245
MvaI CCWGG 2 cut(s) 46, 795
MwoI GCNNNNNNNGC 4 cut(s) 17, 73, 299, 421
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 1 cut(s) 391
NdeII GATC 1 cut(s) 525
NlaIII CATG 4 cut(s) 338, 455, 832, 858
NlaIV GGNNCC 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 3 cut(s) 455, 832, 858
PaqCI CACCTGC 1 cut(s) 317
PceI AGGCCT 2 cut(s) 181, 293
PctI GAATGC 2 cut(s) 22, 245
PdmI GAANNNNTTC 2 cut(s) 94, 960
PfeI GAWTC 3 cut(s) 268, 363, 692
PflMI CCANNNNNTGG 1 cut(s) 902
PkrI GCNGC 2 cut(s) 122, 853
PleI GAGTC 2 cut(s) 279, 442
PpsI GAGTC 2 cut(s) 279, 442
Psp124BI GAGCTC 1 cut(s) 934
Psp1406I AACGTT 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 44, 793
PspGI CCWGG 2 cut(s) 44, 793
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 2 cut(s) 737, 890
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 3 cut(s) 224, 474, 771
SacI GAGCTC 1 cut(s) 934
SaqAI TTAA 3 cut(s) 296, 443, 681
SatI GCNGC 2 cut(s) 121, 852
Sau3AI GATC 1 cut(s) 525
Sau96I GGNCC 2 cut(s) 737, 890
SchI GAGTC 2 cut(s) 279, 443
ScrFI CCNGG 2 cut(s) 46, 795
SduI GDGCHC 2 cut(s) 166, 934
SfaNI GCATC 3 cut(s) 222, 232, 311
SinI GGWCC 2 cut(s) 737, 890
SmiMI CAYNNNNRTG 3 cut(s) 224, 474, 771
SmlI CTYRAG 2 cut(s) 287, 317
SmoI CTYRAG 2 cut(s) 287, 317
Sse9I AATT 6 cut(s) 102, 213, 339, 686, 720, 798
SseBI AGGCCT 2 cut(s) 181, 293
SsiI CCGC 5 cut(s) 58, 121, 185, 518, 852
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 1 cut(s) 934
StuI AGGCCT 2 cut(s) 181, 293
StyD4I CCNGG 2 cut(s) 44, 793
StyI CCWWGG 2 cut(s) 272, 334
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 565
TasI AATT 6 cut(s) 102, 213, 339, 686, 720, 798
TatI WGTACW 1 cut(s) 825
TauI GCSGC 2 cut(s) 123, 854
TfiI GAWTC 3 cut(s) 268, 363, 692
Tru1I TTAA 3 cut(s) 296, 443, 681
Tru9I TTAA 3 cut(s) 296, 443, 681
TscAI CASTG 1 cut(s) 818
TseFI GTSAC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 423, 672
TspRI CASTG 1 cut(s) 818
Van91I CCANNNNNTGG 1 cut(s) 902
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 737, 890
XapI RAATTY 4 cut(s) 102, 213, 686, 720
XceI RCATGY 3 cut(s) 455, 832, 858
XmnI GAANNNNTTC 2 cut(s) 94, 960
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.