AT5G59420

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009849
Physical Location & Seq
Forward (+)
23961567 .. 23964874
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59420.1

Sequence Viewer

Length: 1374 bp
ATGGGAAGTCCAAAGAAGAACGAGAACAAAGGCTTCTTCGCCGCCATGACTTCTGGCTTCTCCATGTTCGGTACCGCCGTGTCGAGATCCGTTAACGGCGTGCAAGGTAATGAAGGAGTTGAGGTCATAAATCCAGAAGGTGGCAAGGAAGATGCTGAAGAGGAAGCTCAGAAAGGAAGGTGGAAGGACGAGGAACGAGATAGTTACTGGAAGATGATGCAGAAATATATAGGTTCGGATATTACGTCAATGGTGACTCTTCCTGTTGTTATATTTGAGCCTATGACTATGCTCCAGAAGATGGCTGAGATAATGGAGTATTCTCATTTGTTGGATCAAGCAGATGAATGCGAAGATCCATACTTGCGTTTAGTATATGCTTCATCATGGGCTATATCTGTTTACTATGCCTTCCAACGAACTTGGAAGCCTTTCAATCCTATTCTTGGGGAGACATATGAGATGGTCAACCATGGTGGGATTTCTTTTATTTCTGAGCAGGTTAGCCATCATCCACCAATGAGTGCTGGTCATGCCGAGAACGAGCACTTCATTTACGACATCACATCAAAGTTGAAAACTAAACTTTTGGGTAACTCTGTTGATGTTTACCCTGTGGGAAGAACGCGTGTAACCCTCAAGAAAGATGGTGTGGTTCTGGATTTGGTGCCGCCTCTCACTAAGATTCACAATCTAATATTTGGACGAACCTGGGTTGACTCACCTGGGGAAATGGTCATGACAAATTTAACCACTGGAGACAAAGTTGTGCTTTATTTCCAGCCATGTGGCTGGTTCGGTTCTGGCCGCTATGAAGTTGATGGCTACGTTTACAGCGCAGCTGAAGAACCGAAAATCATGATGACAGGAAAATGGAATGAGAAAATGAGCTACCAACCTTGTGATGCCGAAGGGGAACCCCTTCCAGGAACAGAGCTGAAAGAGGTGTGGCATTTGGCTGATGTCCCCAAAAACGACAACTTTCAGTACACTCACTTTGCTCACAAGATAAACAGCTTCGACACAGCGCCTGCTAAGCTCTTGGCTTCAGACTCACGTATCCGTCCTGATAGATATTCCCTTGAGCAGGGTGACCTTTCTAAAGCTGGTTCCGAGAAACACAGCCTTGAGGAGAGACAAAGGGCCGAAAAGAGGACCAGAGAGACAAAGGGACAAAAGTTCACTCCAAGATGGTTCGATCTAACGGATGAGATCACACCTACTCCATGGGGAGATATTGAAGTATACCAATACAACGGGAAGTACAATGAACACCGAGACACGGCAGAGAGCTCAAGTAGTGCCTCCAACGAAACGGACCTCAAATCGATCGAGTTTAATCCTTGGCAATATGGTAATATCTCAACCGAATGA

Protein Analysis

457

Amino Acids

51.97

Weight (kDa)

5.18

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 68 - 426 2.2e-111 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 490
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 2 cut(s) 71, 667
AccB7I CCANNNNNTGG 2 cut(s) 140, 301
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 1 cut(s) 628
AciI CCGC 4 cut(s) 42, 75, 671, 808
AclWI GGATC 3 cut(s) 81, 342, 350
AcoI YGGCCR 1 cut(s) 805
AcsI RAATTY 1 cut(s) 745
AcuI CTGAAG 3 cut(s) 177, 864, 1032
AfaI GTAC 3 cut(s) 73, 989, 1265
AfiI CCNNNNNNNGG 7 cut(s) 140, 301, 446, 926, 1087, 1152, 1282
AflIII ACRYGT 1 cut(s) 626
AgsI TTSAA 3 cut(s) 436, 577, 1241
AjnI CCWGG 3 cut(s) 710, 724, 925
AluBI AGCT 8 cut(s) 167, 842, 891, 937, 1017, 1039, 1106, 1293
AluI AGCT 8 cut(s) 167, 842, 891, 937, 1017, 1039, 1106, 1293
Alw21I GWGCWC 2 cut(s) 549, 1295
Alw26I GTCTC 5 cut(s) 446, 753, 1129, 1157, 1272
AlwI GGATC 3 cut(s) 81, 342, 350
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 805, 1143
ApeKI GCWGC 1 cut(s) 839
ApoI RAATTY 1 cut(s) 745
Asp700I GAANNNNTTC 2 cut(s) 431, 921
Asp718I GGTACC 1 cut(s) 71
AspLEI GCGC 2 cut(s) 839, 1030
AspS9I GGNCC 3 cut(s) 1143, 1155, 1318
AsuHPI GGTGA 3 cut(s) 265, 714, 1103
AvaII GGWCC 2 cut(s) 1155, 1318
BaeI ACNNNNGTAYC 4 cut(s) 63, 96, 1042, 1075
BanI GGYRCC 2 cut(s) 71, 667
BanII GRGCYC 1 cut(s) 1295
Bbv12I GWGCWC 2 cut(s) 549, 1295
BbvI GCAGC 1 cut(s) 851
BccI CCATC 6 cut(s) 295, 457, 516, 641, 815, 1185
BceAI ACGGC 3 cut(s) 62, 112, 1299
BciT130I CCWGG 3 cut(s) 712, 726, 927
BciVI GTATCC 1 cut(s) 1070
BcoDI GTCTC 5 cut(s) 446, 753, 1129, 1157, 1272
BfoI RGCGCY 1 cut(s) 1031
BfuAI ACCTGC 1 cut(s) 490
BfuI GTATCC 1 cut(s) 1070
BisI GCNGC 4 cut(s) 42, 671, 808, 840
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 4 cut(s) 43, 672, 809, 841
Bme1390I CCNGG 3 cut(s) 712, 726, 927
Bme18I GGWCC 2 cut(s) 1155, 1318
BmgT120I GGNCC 3 cut(s) 1143, 1155, 1318
BmiI GGNNCC 4 cut(s) 73, 669, 918, 1111
BmrFI CCNGG 3 cut(s) 712, 726, 927
BmsI GCATC 3 cut(s) 142, 207, 895
BpmI CTGGAG 2 cut(s) 278, 777
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 4 cut(s) 623, 1103, 1148, 1279
Bsa29I ATCGAT 1 cut(s) 1328
BsaAI YACGTR 1 cut(s) 1058
BsaJI CCNNGG 5 cut(s) 472, 711, 725, 1226, 1343
BsaXI ACNNNNNCTCC 6 cut(s) 108, 138, 750, 780, 1207, 1237
Bsc4I CCNNNNNNNGG 7 cut(s) 140, 301, 446, 926, 1087, 1152, 1282
Bse1I ACTGG 2 cut(s) 212, 760
BseBI CCWGG 3 cut(s) 712, 726, 927
BseCI ATCGAT 1 cut(s) 1328
BseDI CCNNGG 5 cut(s) 472, 711, 725, 1226, 1343
BseGI GGATG 2 cut(s) 511, 1213
BseLI CCNNNNNNNGG 7 cut(s) 140, 301, 446, 926, 1087, 1152, 1282
BseMII CTCAG 3 cut(s) 182, 297, 486
BseNI ACTGG 2 cut(s) 212, 760
BseRI GAGGAG 1 cut(s) 1145
BseXI GCAGC 1 cut(s) 851
Bsh1236I CGCG 1 cut(s) 628
Bsh1285I CGRYCG 1 cut(s) 1332
BshFI GGCC 2 cut(s) 807, 1145
BshNI GGYRCC 2 cut(s) 71, 667
BshVI ATCGAT 1 cut(s) 1328
BsiEI CGRYCG 1 cut(s) 1332
BsiHKAI GWGCWC 2 cut(s) 549, 1295
BslFI GGGAC 2 cut(s) 950, 1185
BslI CCNNNNNNNGG 7 cut(s) 140, 301, 446, 926, 1087, 1152, 1282
BsmAI GTCTC 5 cut(s) 446, 753, 1129, 1157, 1272
BsmFI GGGAC 2 cut(s) 950, 1185
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 2 cut(s) 807, 1145
Bsp1286I GDGCHC 2 cut(s) 549, 1295
Bsp143I GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
Bsp1720I GCTNAGC 1 cut(s) 1035
Bsp19I CCATGG 2 cut(s) 472, 1226
BspACI CCGC 4 cut(s) 42, 75, 671, 808
BspANI GGCC 2 cut(s) 807, 1145
BspCNI CTCAG 3 cut(s) 181, 298, 487
BspDI ATCGAT 1 cut(s) 1328
BspFNI CGCG 1 cut(s) 628
BspHI TCATGA 2 cut(s) 738, 858
BspLI GGNNCC 4 cut(s) 73, 669, 918, 1111
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 3 cut(s) 81, 342, 350
BspT107I GGYRCC 2 cut(s) 71, 667
BsrI ACTGG 2 cut(s) 212, 760
BssECI CCNNGG 5 cut(s) 472, 711, 725, 1226, 1343
BssMI GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
BssNAI GTATAC 1 cut(s) 1246
BssT1I CCWWGG 3 cut(s) 472, 1226, 1343
Bst1107I GTATAC 1 cut(s) 1246
Bst2UI CCWGG 3 cut(s) 712, 726, 927
Bst6I CTCTTC 2 cut(s) 153, 264
BstBAI YACGTR 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 101, 1032
BstDEI CTNAG 5 cut(s) 168, 306, 495, 681, 1035
BstDSI CCRYGG 2 cut(s) 472, 1226
BstEII GGTNACC 1 cut(s) 1091
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 2 cut(s) 511, 1213
BstFNI CGCG 1 cut(s) 628
BstH2I RGCGCY 1 cut(s) 1031
BstHHI GCGC 2 cut(s) 839, 1030
BstKTI GATC 6 cut(s) 89, 337, 358, 1201, 1215, 1332
BstMAI GTCTC 5 cut(s) 446, 753, 1129, 1157, 1272
BstMBI GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
BstMCI CGRYCG 1 cut(s) 1332
BstMWI GCNNNNNNNGC 2 cut(s) 533, 1036
BstNI CCWGG 3 cut(s) 712, 726, 927
BstPI GGTNACC 1 cut(s) 1091
BstSCI CCNGG 3 cut(s) 710, 724, 925
BstUI CGCG 1 cut(s) 628
BstV1I GCAGC 1 cut(s) 851
BstX2I RGATCY 2 cut(s) 86, 355
BstXI CCANNNNNNTGG 2 cut(s) 788, 792
BstYI RGATCY 2 cut(s) 86, 355
BstZ17I GTATAC 1 cut(s) 1246
Bsu15I ATCGAT 1 cut(s) 1328
BsuI GTATCC 1 cut(s) 1070
BsuRI GGCC 2 cut(s) 807, 1145
BsuTUI ATCGAT 1 cut(s) 1328
BtgI CCRYGG 2 cut(s) 472, 1226
BtsCI GGATG 2 cut(s) 511, 1213
BtsIMutI CAGTG 1 cut(s) 753
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 101, 1032
CaiI CAGNNNCTG 1 cut(s) 1031
CciI TCATGA 2 cut(s) 738, 858
CfoI GCGC 2 cut(s) 839, 1030
Cfr13I GGNCC 3 cut(s) 1143, 1155, 1318
ClaI ATCGAT 1 cut(s) 1328
Csp6I GTAC 3 cut(s) 72, 988, 1264
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 9 cut(s) 46, 64, 387, 473, 533, 739, 786, 859, 1227
CviQI GTAC 3 cut(s) 72, 988, 1264
DdeI CTNAG 5 cut(s) 168, 306, 495, 681, 1035
DpnI GATC 6 cut(s) 88, 336, 357, 1200, 1214, 1331
DpnII GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
EaeI YGGCCR 1 cut(s) 805
Eam1104I CTCTTC 2 cut(s) 153, 264
EarI CTCTTC 2 cut(s) 153, 264
Ecl136II GAGCTC 1 cut(s) 1293
Eco130I CCWWGG 3 cut(s) 472, 1226, 1343
Eco24I GRGCYC 1 cut(s) 1295
Eco47I GGWCC 2 cut(s) 1155, 1318
Eco53kI GAGCTC 1 cut(s) 1293
Eco57I CTGAAG 3 cut(s) 177, 864, 1032
Eco91I GGTNACC 1 cut(s) 1091
EcoICRI GAGCTC 1 cut(s) 1293
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoO65I GGTNACC 1 cut(s) 1091
EcoRII CCWGG 3 cut(s) 710, 724, 925
EcoT14I CCWWGG 3 cut(s) 472, 1226, 1343
EcoT38I GRGCYC 1 cut(s) 1295
ErhI CCWWGG 3 cut(s) 472, 1226, 1343
FaeI CATG 9 cut(s) 49, 67, 390, 476, 536, 742, 789, 862, 1230
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 2 cut(s) 950, 1185
FatI CATG 9 cut(s) 45, 63, 386, 472, 532, 738, 785, 858, 1226
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 4 cut(s) 42, 671, 808, 840
FokI GGATG 2 cut(s) 498, 1220
FriOI GRGCYC 1 cut(s) 1295
Fsp4HI GCNGC 4 cut(s) 42, 671, 808, 840
GlaI GCGC 2 cut(s) 838, 1029
GluI GCNGC 4 cut(s) 42, 671, 808, 840
GsuI CTGGAG 2 cut(s) 278, 777
HaeII RGCGCY 1 cut(s) 1031
HaeIII GGCC 2 cut(s) 807, 1145
HhaI GCGC 2 cut(s) 839, 1030
Hin1II CATG 9 cut(s) 49, 67, 390, 476, 536, 742, 789, 862, 1230
Hin6I GCGC 2 cut(s) 837, 1028
HinP1I GCGC 2 cut(s) 837, 1028
HincII GTYRAC 3 cut(s) 94, 469, 718
HindII GTYRAC 3 cut(s) 94, 469, 718
HinfI GANTC 4 cut(s) 256, 685, 719, 1052
HpaI GTTAAC 1 cut(s) 94
HphI GGTGA 3 cut(s) 265, 714, 1103
Hpy166II GTNNAC 9 cut(s) 94, 403, 469, 610, 718, 832, 990, 1182, 1246
Hpy188I TCNGA 5 cut(s) 171, 238, 496, 1051, 1114
Hpy188III TCNNGA 8 cut(s) 84, 134, 295, 640, 659, 739, 859, 1067
Hpy8I GTNNAC 9 cut(s) 94, 403, 469, 610, 718, 832, 990, 1182, 1246
HpyAV CCTTC 7 cut(s) 107, 131, 171, 178, 421, 905, 932
HpyCH4IV ACGT 3 cut(s) 245, 828, 1057
HpyCH4V TGCA 2 cut(s) 103, 220
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 1036
HpyF3I CTNAG 5 cut(s) 168, 306, 495, 681, 1035
HpySE526I ACGT 3 cut(s) 245, 828, 1057
Hsp92II CATG 9 cut(s) 49, 67, 390, 476, 536, 742, 789, 862, 1230
HspAI GCGC 2 cut(s) 837, 1028
KpnI GGTACC 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
LmnI GCTCC 1 cut(s) 297
Lsp1109I GCAGC 1 cut(s) 851
LweI GCATC 3 cut(s) 142, 207, 895
MaeII ACGT 3 cut(s) 245, 828, 1057
MaeIII GTNAC 5 cut(s) 203, 253, 593, 631, 1091
MalI GATC 6 cut(s) 88, 336, 357, 1200, 1214, 1331
MboI GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
MflI RGATCY 2 cut(s) 86, 355
MhlI GDGCHC 2 cut(s) 549, 1295
MluCI AATT 1 cut(s) 745
MluI ACGCGT 1 cut(s) 626
MlyI GAGTC 3 cut(s) 250, 713, 1046
MmeI TCCRAC 3 cut(s) 312, 439, 1332
MroXI GAANNNNTTC 2 cut(s) 431, 921
MseI TTAA 3 cut(s) 93, 749, 1338
MspA1I CMGCKG 1 cut(s) 842
MspR9I CCNGG 3 cut(s) 712, 726, 927
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 3 cut(s) 712, 726, 927
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 2 cut(s) 533, 1036
NcoI CCATGG 2 cut(s) 472, 1226
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
NlaIII CATG 9 cut(s) 49, 67, 390, 476, 536, 742, 789, 862, 1230
NlaIV GGNNCC 4 cut(s) 73, 669, 918, 1111
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 2 cut(s) 253, 1091
PagI TCATGA 2 cut(s) 738, 858
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 242
PctI GAATGC 1 cut(s) 353
PdmI GAANNNNTTC 2 cut(s) 431, 921
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 2 cut(s) 140, 301
PfoI TCCNGGA 1 cut(s) 925
PkrI GCNGC 4 cut(s) 43, 672, 809, 841
Ple19I CGATCG 1 cut(s) 1332
PleI GAGTC 3 cut(s) 250, 713, 1046
PpsI GAGTC 3 cut(s) 250, 713, 1046
Ppu21I YACGTR 1 cut(s) 1058
Psp124BI GAGCTC 1 cut(s) 1295
Psp6I CCWGG 3 cut(s) 710, 724, 925
PspEI GGTNACC 1 cut(s) 1091
PspGI CCWGG 3 cut(s) 710, 724, 925
PspN4I GGNNCC 4 cut(s) 73, 669, 918, 1111
PspPI GGNCC 3 cut(s) 1143, 1155, 1318
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 2 cut(s) 86, 355
PvuI CGATCG 1 cut(s) 1332
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 3 cut(s) 73, 989, 1265
RsaNI GTAC 3 cut(s) 72, 988, 1264
SacI GAGCTC 1 cut(s) 1295
SaqAI TTAA 3 cut(s) 93, 749, 1338
SatI GCNGC 4 cut(s) 42, 671, 808, 840
Sau3AI GATC 6 cut(s) 86, 334, 355, 1198, 1212, 1329
Sau96I GGNCC 3 cut(s) 1143, 1155, 1318
SchI GAGTC 3 cut(s) 250, 713, 1046
ScrFI CCNGG 3 cut(s) 712, 726, 927
SduI GDGCHC 2 cut(s) 549, 1295
SfaNI GCATC 3 cut(s) 142, 207, 895
SinI GGWCC 2 cut(s) 1155, 1318
SmlI CTYRAG 4 cut(s) 638, 1082, 1127, 1294
SmoI CTYRAG 4 cut(s) 638, 1082, 1127, 1294
Sse9I AATT 1 cut(s) 745
SsiI CCGC 4 cut(s) 42, 75, 671, 808
SspI AATATT 1 cut(s) 699
SstI GAGCTC 1 cut(s) 1295
StyD4I CCNGG 3 cut(s) 710, 724, 925
StyI CCWWGG 3 cut(s) 472, 1226, 1343
TaiI ACGT 3 cut(s) 248, 831, 1060
TaqI TCGA 5 cut(s) 83, 1020, 1197, 1328, 1332
TasI AATT 1 cut(s) 745
TatI WGTACW 2 cut(s) 987, 1263
TauI GCSGC 3 cut(s) 44, 673, 810
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 3 cut(s) 93, 749, 1338
Tru9I TTAA 3 cut(s) 93, 749, 1338
TscAI CASTG 1 cut(s) 760
TseFI GTSAC 2 cut(s) 253, 1091
TseI GCWGC 1 cut(s) 839
Tsp45I GTSAC 2 cut(s) 253, 1091
TspDTI ATGAA 6 cut(s) 126, 360, 372, 541, 828, 1284
TspGWI ACGGA 4 cut(s) 79, 1052, 1220, 1331
TspRI CASTG 1 cut(s) 760
Van91I CCANNNNNTGG 2 cut(s) 140, 301
VpaK11BI GGWCC 2 cut(s) 1155, 1318
XagI CCTNNNNNAGG 1 cut(s) 1085
XapI RAATTY 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 1245
XmnI GAANNNNTTC 2 cut(s) 431, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.