Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0009849

Physical Location & Seq

Genomic Coordinates Forward (+)

50006328 .. 50016414

Gene Structure 10087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0371500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGCTTCCATGTCTCCTCTGTTTGGACATTTGCAAGGAACAAGAAAGGAAAATTTCTGAAGGGCCATCACATAGAGAGAATAACAGGGATGATGAGAGGATAGAGAACTTGGCCAGGAGATCAAATGACCAATCTGAACAATGTTGTGGGAGTAATGGGATATGCTCTCATGAAGTTCTTCGAGATGGTGAATGTGATACTGATTCAAAATCTCATGAAGTTCTTCGAGATGGTGAATGTGATACTGATTCAAAATCGCTTCTCTATAAGTTTGCAAGAAAGGCATCATTCAAACTTTCATGGTGCTGCAAAGGTGACCAGAGTGACCAGCACAAGCATGACATTGTCTCTTTTGAAAGAGGAAACATAACAACTGCAGAGCGCAGCAGTAAACAGATTTCTTTGAAGTGGGAAACTCACCCACGGACTGTGCTTATATTGACCAAACCAAATTCAACTTCTGTTCGAATTCTGTGTGCAGAAATGGTCAGATGGTTGAGAGAGCAGAAAAAGTTAGATATTTATGTGGAGCCACGTGTGAGAGTTGAACTTGTGACAGAGTCATCATACTACAACTTCGTCCAGACATGGAAAGAAGACACTGAGATTATGCTCCTGCATACAAAAGTTGACCTTGTGGTAACTCTTGGTGGGGATGGAACTGTCCTCTGGGCAGCATCCATGTTCAAAGGACCAGTTCCTCCTGTTGTGCCATTTTCTTTAGGATCTCTGGGATTTATGACTCCTTTTCACAGTGAACAGTATCAGGATTATCTTGATTCACTTTTAAAAGGTCCCATTAGTATCACGCTACGGCACCGGTTACAGTGCCATGTTATTAGAGAAGCAGCTAAAAATGAGTACGAAACTGAAGGGCCAATACTTGTTTTAAATGAGGTTACAATTGACCGTGGGATATCATCATATCTCACAAATTTGGAATGCTATTGTGACAACTCTTTTGTCACATGCGTCCAAGGAGATGGGCTGATATTATCGACACCATCTGGCAGTACTGCGTATTCATTAGCAGCTGGAGGATCAATGGTCCATCCCCAGGTTCCAGGCATCCTCTTTACCCCTATTTGTCCTCATTCCCTATCGTTCCGGCCTCTGATATTACCTGAGTATGTGACATTGCGAGTACAAGTACCTTATAACAGCAGAAGCCATGCATGGGCATCATTTGATGGGAAGGACAGGAAACGACTGGCAGCTGGTGATGCACTCGTGTGCAGTATGGCTACTTGGCCCGTCCCCACTGCATGTCAAGTTGATTCAACTAGTGATTTCTTAACTAGCATTCATGATGGCCTGCACTGGAATCTGAGAAAGACTCAATCTCATGATGGCCCAAGGGATATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Oxysterol_BP

Protein Analysis

455

Amino Acids

51.18

Weight (kDa)

5.99

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_kinase	PF01513	144 - 265	4.9e-19	ATP-NAD kinase N-terminal domain
NAD_kinase_C	PF20143	297 - 412	1e-31	ATP-NAD kinase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009849)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59420
fragaria_vesca	FvH4_3g36500 FvH4_3g36500
malus_domestica	MD03G1090000.v1.1 MD11G1099300.v1.1
prunus_persica	Prupe.6G074200_v2.0.a1 Prupe.6G074200_v2.0.a1
pyrus_communis	pycom03g07160 pycom11g08370
rosa_chinensis	RchiOBHm_Chr5g0065361 RchiOBHm_Chr5g0065411
rosa_laevigata	RLG00000035786
rosa_multiflora	Rmu_sc0009942.1_g000003
rosa_roxburghii	Rroxscaffold_1G00015830
rosa_rugosa	Rorug05G0371500 Rorug05G0371500 Rorug05G0371600
rosa_samantha	Rh5BG444700 Rh5CG467000 Rh5DG457000
rosa_wichuraiana	Rw5G040310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1158
AccB1I	GGYRCC	1 cut(s)	816
AclWI	GGATC	2 cut(s)	733, 1048
AcoI	YGGCCR	1 cut(s)	111
AcsI	RAATTY	4 cut(s)	51, 451, 468, 934
AcuI	CTGAAG	2 cut(s)	78, 891
AcvI	CACGTG	1 cut(s)	536
AfaI	GTAC	4 cut(s)	863, 1015, 1146, 1152
AfiI	CCNNNNNNNGG	3 cut(s)	22, 1057, 1177
AflIII	ACRYGT	1 cut(s)	535
AgeI	ACCGGT	1 cut(s)	819
AgsI	TTSAA	9 cut(s)	207, 252, 292, 356, 406, 456, 548, 688, 1281
AhlI	ACTAGT	1 cut(s)	1283
AjnI	CCWGG	3 cut(s)	113, 1056, 1063
AjuI	GAANNNNNNNTTGG	2 cut(s)	442, 474
AleI	CACNNNNGTG	1 cut(s)	1231
AluBI	AGCT	3 cut(s)	851, 1034, 1217
AluI	AGCT	3 cut(s)	851, 1034, 1217
Alw26I	GTCTC	2 cut(s)	17, 352
AlwI	GGATC	2 cut(s)	733, 1048
AoxI	GGCC	7 cut(s)	62, 111, 875, 1109, 1250, 1312, 1351
ApeKI	GCWGC	6 cut(s)	306, 384, 674, 848, 1031, 1214
ApoI	RAATTY	4 cut(s)	51, 451, 468, 934
ArsI	GACNNNNNNTTYG	2 cut(s)	944, 976
AsiGI	ACCGGT	1 cut(s)	819
Asp700I	GAANNNNTTC	2 cut(s)	177, 222
AspLEI	GCGC	1 cut(s)	384
AspS9I	GGNCC	7 cut(s)	62, 692, 794, 875, 1048, 1251, 1352
AsuHPI	GGTGA	5 cut(s)	200, 245, 326, 410, 1232
AsuII	TTCGAA	1 cut(s)	466
AvaII	GGWCC	3 cut(s)	692, 794, 1048
BalI	TGGCCA	1 cut(s)	113
BanI	GGYRCC	1 cut(s)	816
BarI	GAAGNNNNNNTAC	2 cut(s)	864, 896
BauI	CACGAG	1 cut(s)	1229
BbrPI	CACGTG	1 cut(s)	536
BbsI	GAAGAC	1 cut(s)	603
BbvI	GCAGC	6 cut(s)	293, 396, 686, 860, 1043, 1226
BceAI	ACGGC	1 cut(s)	830
BciT130I	CCWGG	3 cut(s)	115, 1058, 1065
BcoDI	GTCTC	2 cut(s)	17, 352
BcuI	ACTAGT	1 cut(s)	1283
BfaI	CTAG	2 cut(s)	1284, 1299
BfmI	CTRYAG	1 cut(s)	375
BisI	GCNGC	6 cut(s)	307, 385, 675, 849, 1032, 1215
BlsI	GCNGC	6 cut(s)	308, 386, 676, 850, 1033, 1216
BmcAI	AGTACT	1 cut(s)	1015
Bme1390I	CCNGG	3 cut(s)	115, 1058, 1065
Bme18I	GGWCC	3 cut(s)	692, 794, 1048
BmgT120I	GGNCC	7 cut(s)	62, 692, 794, 875, 1048, 1251, 1352
BmiI	GGNNCC	4 cut(s)	531, 796, 818, 1062
BmrFI	CCNGG	3 cut(s)	115, 1058, 1065
BmsI	GCATC	5 cut(s)	293, 686, 1077, 1190, 1213
BpiI	GAAGAC	1 cut(s)	603
BplI	GAGNNNNNCTC	2 cut(s)	1321, 1353
BpmI	CTGGAG	1 cut(s)	1056
Bpu14I	TTCGAA	1 cut(s)	466
BsaAI	YACGTR	1 cut(s)	536
BsaJI	CCNNGG	5 cut(s)	422, 910, 976, 1056, 1355
BsaWI	WCCGGW	1 cut(s)	819
Bsc4I	CCNNNNNNNGG	3 cut(s)	22, 1057, 1177
Bse118I	RCCGGY	1 cut(s)	819
Bse1I	ACTGG	3 cut(s)	695, 1215, 1325
Bse3DI	GCAATG	1 cut(s)	1136
BseBI	CCWGG	3 cut(s)	115, 1058, 1065
BseDI	CCNNGG	5 cut(s)	422, 910, 976, 1056, 1355
BseGI	GGATG	5 cut(s)	94, 661, 677, 1051, 1068
BseLI	CCNNNNNNNGG	3 cut(s)	22, 1057, 1177
BseMI	GCAATG	1 cut(s)	1136
BseMII	CTCAG	3 cut(s)	594, 1116, 1319
BseNI	ACTGG	3 cut(s)	695, 1215, 1325
BseRI	GAGGAG	1 cut(s)	5
BseXI	GCAGC	6 cut(s)	293, 396, 686, 860, 1043, 1226
BsgI	GTGCAG	3 cut(s)	498, 1255, 1301
BshFI	GGCC	7 cut(s)	64, 113, 877, 1111, 1252, 1314, 1353
BshNI	GGYRCC	1 cut(s)	816
BshTI	ACCGGT	1 cut(s)	819
BsiSI	CCGG	2 cut(s)	820, 1108
BslFI	GGGAC	2 cut(s)	780, 1241
BslI	CCNNNNNNNGG	3 cut(s)	22, 1057, 1177
BsmAI	GTCTC	2 cut(s)	17, 352
BsmFI	GGGAC	2 cut(s)	780, 1241
BsmI	GAATGC	2 cut(s)	947, 1302
BsnI	GGCC	7 cut(s)	64, 113, 877, 1111, 1252, 1314, 1353
Bsp119I	TTCGAA	1 cut(s)	466
Bsp143I	GATC	3 cut(s)	119, 725, 1040
BspANI	GGCC	7 cut(s)	64, 113, 877, 1111, 1252, 1314, 1353
BspCNI	CTCAG	3 cut(s)	595, 1117, 1320
BspHI	TCATGA	4 cut(s)	169, 214, 1306, 1345
BspLI	GGNNCC	4 cut(s)	531, 796, 818, 1062
BspMAI	CTGCAG	1 cut(s)	379
BspPI	GGATC	2 cut(s)	733, 1048
BspT104I	TTCGAA	1 cut(s)	466
BspT107I	GGYRCC	1 cut(s)	816
BsrDI	GCAATG	1 cut(s)	1136
BsrFI	RCCGGY	1 cut(s)	819
BsrI	ACTGG	3 cut(s)	695, 1215, 1325
BssAI	RCCGGY	1 cut(s)	819
BssECI	CCNNGG	5 cut(s)	422, 910, 976, 1056, 1355
BssMI	GATC	3 cut(s)	119, 725, 1040
BssSI	CACGAG	1 cut(s)	1229
BssT1I	CCWWGG	2 cut(s)	976, 1355
Bst2BI	CACGAG	1 cut(s)	1229
Bst2UI	CCWGG	3 cut(s)	115, 1058, 1065
Bst4CI	ACNGT	6 cut(s)	430, 664, 755, 762, 828, 911
BstBAI	YACGTR	1 cut(s)	536
BstBI	TTCGAA	1 cut(s)	466
BstC8I	GCNNGC	1 cut(s)	1316
BstDEI	CTNAG	3 cut(s)	603, 1125, 1328
BstDSI	CCRYGG	2 cut(s)	422, 910
BstEII	GGTNACC	1 cut(s)	314
BstF5I	GGATG	5 cut(s)	94, 661, 677, 1051, 1068
BstHHI	GCGC	1 cut(s)	384
BstKTI	GATC	3 cut(s)	122, 728, 1043
BstMAI	GTCTC	2 cut(s)	17, 352
BstMBI	GATC	3 cut(s)	119, 725, 1040
BstMWI	GCNNNNNNNGC	2 cut(s)	281, 1223
BstNI	CCWGG	3 cut(s)	115, 1058, 1065
BstNSI	RCATGY	2 cut(s)	972, 1269
BstPI	GGTNACC	1 cut(s)	314
BstSCI	CCNGG	3 cut(s)	113, 1056, 1063
BstSFI	CTRYAG	1 cut(s)	375
BstV1I	GCAGC	6 cut(s)	293, 396, 686, 860, 1043, 1226
BstV2I	GAAGAC	1 cut(s)	603
BstX2I	RGATCY	1 cut(s)	725
BstXI	CCANNNNNNTGG	1 cut(s)	983
BstYI	RGATCY	1 cut(s)	725
BsuRI	GGCC	7 cut(s)	64, 113, 877, 1111, 1252, 1314, 1353
BtgI	CCRYGG	2 cut(s)	422, 910
BtsCI	GGATG	5 cut(s)	94, 661, 677, 1051, 1068
BtsI	GCAGTG	1 cut(s)	1260
BtsIMutI	CAGTG	5 cut(s)	600, 760, 833, 1260, 1318
Cac8I	GCNNGC	1 cut(s)	1316
CciI	TCATGA	4 cut(s)	169, 214, 1306, 1345
CfoI	GCGC	1 cut(s)	384
Cfr10I	RCCGGY	1 cut(s)	819
Cfr13I	GGNCC	7 cut(s)	62, 692, 794, 875, 1048, 1251, 1352
CseI	GACGC	1 cut(s)	961
Csp6I	GTAC	4 cut(s)	862, 1014, 1145, 1151
CspAI	ACCGGT	1 cut(s)	819
CviQI	GTAC	4 cut(s)	862, 1014, 1145, 1151
DdeI	CTNAG	3 cut(s)	603, 1125, 1328
DpnI	GATC	3 cut(s)	121, 727, 1042
DpnII	GATC	3 cut(s)	119, 725, 1040
DraI	TTTAAA	2 cut(s)	789, 891
EaeI	YGGCCR	1 cut(s)	111
Eco130I	CCWWGG	2 cut(s)	976, 1355
Eco32I	GATATC	1 cut(s)	918
Eco47I	GGWCC	3 cut(s)	692, 794, 1048
Eco57I	CTGAAG	2 cut(s)	78, 891
Eco72I	CACGTG	1 cut(s)	536
Eco91I	GGTNACC	1 cut(s)	314
EcoO109I	RGGNCCY	1 cut(s)	794
EcoO65I	GGTNACC	1 cut(s)	314
EcoRI	GAATTC	1 cut(s)	468
EcoRII	CCWGG	3 cut(s)	113, 1056, 1063
EcoRV	GATATC	1 cut(s)	918
EcoT14I	CCWWGG	2 cut(s)	976, 1355
EcoT22I	ATGCAT	1 cut(s)	1177
ErhI	CCWWGG	2 cut(s)	976, 1355
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	2 cut(s)	780, 1241
Fnu4HI	GCNGC	6 cut(s)	307, 385, 675, 849, 1032, 1215
FokI	GGATG	5 cut(s)	101, 664, 668, 1038, 1055
Fsp4HI	GCNGC	6 cut(s)	307, 385, 675, 849, 1032, 1215
FspBI	CTAG	2 cut(s)	1284, 1299
GlaI	GCGC	1 cut(s)	383
GluI	GCNGC	6 cut(s)	307, 385, 675, 849, 1032, 1215
GsuI	CTGGAG	1 cut(s)	1056
HaeIII	GGCC	7 cut(s)	64, 113, 877, 1111, 1252, 1314, 1353
HapII	CCGG	2 cut(s)	820, 1108
HgaI	GACGC	1 cut(s)	961
HhaI	GCGC	1 cut(s)	384
Hin6I	GCGC	1 cut(s)	382
HinP1I	GCGC	1 cut(s)	382
HincII	GTYRAC	1 cut(s)	631
HindII	GTYRAC	1 cut(s)	631
HinfI	GANTC	8 cut(s)	203, 248, 560, 742, 779, 1277, 1324, 1336
HpaII	CCGG	2 cut(s)	820, 1108
HphI	GGTGA	5 cut(s)	200, 245, 326, 410, 1232
Hpy166II	GTNNAC	3 cut(s)	392, 631, 758
Hpy188I	TCNGA	5 cut(s)	58, 136, 491, 1116, 1329
Hpy188III	TCNNGA	9 cut(s)	170, 182, 215, 227, 583, 767, 776, 1307, 1346
Hpy8I	GTNNAC	3 cut(s)	392, 631, 758
HpyAV	CCTTC	3 cut(s)	53, 866, 1189
HpyCH4III	ACNGT	6 cut(s)	430, 664, 755, 762, 828, 911
HpyCH4IV	ACGT	1 cut(s)	535
HpyF10VI	GCNNNNNNNGC	2 cut(s)	281, 1223
HpyF3I	CTNAG	3 cut(s)	603, 1125, 1328
HpySE526I	ACGT	1 cut(s)	535
HspAI	GCGC	1 cut(s)	382
Kzo9I	GATC	3 cut(s)	119, 725, 1040
LmnI	GCTCC	2 cut(s)	529, 618
Lsp1109I	GCAGC	6 cut(s)	293, 396, 686, 860, 1043, 1226
LweI	GCATC	5 cut(s)	293, 686, 1077, 1190, 1213
MaeI	CTAG	2 cut(s)	1284, 1299
MaeII	ACGT	1 cut(s)	535
MaeIII	GTNAC	9 cut(s)	314, 323, 553, 640, 822, 898, 950, 964, 1132
MalI	GATC	3 cut(s)	121, 727, 1042
MboI	GATC	3 cut(s)	119, 725, 1040
MboII	GAAGA	3 cut(s)	170, 215, 608
MfeI	CAATTG	1 cut(s)	903
MflI	RGATCY	1 cut(s)	725
MlsI	TGGCCA	1 cut(s)	113
MluCI	AATT	5 cut(s)	51, 451, 468, 903, 934
MluNI	TGGCCA	1 cut(s)	113
MlyI	GAGTC	3 cut(s)	569, 736, 1330
Mox20I	TGGCCA	1 cut(s)	113
Mph1103I	ATGCAT	1 cut(s)	1177
MroXI	GAANNNNTTC	2 cut(s)	177, 222
MscI	TGGCCA	1 cut(s)	113
MseI	TTAA	3 cut(s)	788, 890, 1295
MslI	CAYNNNNRTG	2 cut(s)	336, 1231
Msp20I	TGGCCA	1 cut(s)	113
MspA1I	CMGCKG	2 cut(s)	1034, 1217
MspI	CCGG	2 cut(s)	820, 1108
MspR9I	CCNGG	3 cut(s)	115, 1058, 1065
MunI	CAATTG	1 cut(s)	903
Mva1269I	GAATGC	2 cut(s)	947, 1302
MvaI	CCWGG	3 cut(s)	115, 1058, 1065
MwoI	GCNNNNNNNGC	2 cut(s)	281, 1223
NdeII	GATC	3 cut(s)	119, 725, 1040
NlaIV	GGNNCC	4 cut(s)	531, 796, 818, 1062
NmuCI	GTSAC	6 cut(s)	314, 323, 553, 950, 964, 1132
NsiI	ATGCAT	1 cut(s)	1177
NspI	RCATGY	2 cut(s)	972, 1269
NspV	TTCGAA	1 cut(s)	466
OliI	CACNNNNGTG	1 cut(s)	1231
PagI	TCATGA	4 cut(s)	169, 214, 1306, 1345
PctI	GAATGC	2 cut(s)	947, 1302
PdmI	GAANNNNTTC	2 cut(s)	177, 222
PfeI	GAWTC	5 cut(s)	203, 248, 779, 1277, 1324
PflFI	GACNNNGTC	2 cut(s)	344, 559
PinAI	ACCGGT	1 cut(s)	819
PkrI	GCNGC	6 cut(s)	308, 386, 676, 850, 1033, 1216
PleI	GAGTC	3 cut(s)	568, 736, 1330
PmaCI	CACGTG	1 cut(s)	536
PmlI	CACGTG	1 cut(s)	536
PpsI	GAGTC	3 cut(s)	568, 736, 1330
Ppu21I	YACGTR	1 cut(s)	536
PpuMI	RGGWCCY	1 cut(s)	794
PsiI	TTATAA	1 cut(s)	1158
Psp5II	RGGWCCY	1 cut(s)	794
Psp6I	CCWGG	3 cut(s)	113, 1056, 1063
PspCI	CACGTG	1 cut(s)	536
PspEI	GGTNACC	1 cut(s)	314
PspGI	CCWGG	3 cut(s)	113, 1056, 1063
PspN4I	GGNNCC	4 cut(s)	531, 796, 818, 1062
PspPI	GGNCC	7 cut(s)	62, 692, 794, 875, 1048, 1251, 1352
PspPPI	RGGWCCY	1 cut(s)	794
PstI	CTGCAG	1 cut(s)	379
PsuI	RGATCY	1 cut(s)	725
PsyI	GACNNNGTC	2 cut(s)	344, 559
PvuII	CAGCTG	2 cut(s)	1034, 1217
RsaI	GTAC	4 cut(s)	863, 1015, 1146, 1152
RsaNI	GTAC	4 cut(s)	862, 1014, 1145, 1151
RseI	CAYNNNNRTG	2 cut(s)	336, 1231
SaqAI	TTAA	3 cut(s)	788, 890, 1295
SatI	GCNGC	6 cut(s)	307, 385, 675, 849, 1032, 1215
Sau3AI	GATC	3 cut(s)	119, 725, 1040
Sau96I	GGNCC	7 cut(s)	62, 692, 794, 875, 1048, 1251, 1352
ScaI	AGTACT	1 cut(s)	1015
SchI	GAGTC	3 cut(s)	569, 736, 1330
ScrFI	CCNGG	3 cut(s)	115, 1058, 1065
SfaNI	GCATC	5 cut(s)	293, 686, 1077, 1190, 1213
SfcI	CTRYAG	1 cut(s)	375
SfuI	TTCGAA	1 cut(s)	466
SinI	GGWCC	3 cut(s)	692, 794, 1048
SmiMI	CAYNNNNRTG	2 cut(s)	336, 1231
SpeI	ACTAGT	1 cut(s)	1283
Sse9I	AATT	5 cut(s)	51, 451, 468, 903, 934
SspMI	CTAG	2 cut(s)	1284, 1299
StyD4I	CCNGG	3 cut(s)	113, 1056, 1063
StyI	CCWWGG	2 cut(s)	976, 1355
TaaI	ACNGT	6 cut(s)	430, 664, 755, 762, 828, 911
TaiI	ACGT	1 cut(s)	538
TaqI	TCGA	4 cut(s)	181, 226, 466, 998
TasI	AATT	5 cut(s)	51, 451, 468, 903, 934
TatI	WGTACW	2 cut(s)	1013, 1144
TfiI	GAWTC	5 cut(s)	203, 248, 779, 1277, 1324
Tru1I	TTAA	3 cut(s)	788, 890, 1295
Tru9I	TTAA	3 cut(s)	788, 890, 1295
TscAI	CASTG	5 cut(s)	607, 760, 833, 1267, 1325
TseFI	GTSAC	6 cut(s)	314, 323, 553, 950, 964, 1132
TseI	GCWGC	6 cut(s)	306, 384, 674, 848, 1031, 1214
Tsp45I	GTSAC	6 cut(s)	314, 323, 553, 950, 964, 1132
TspDTI	ATGAA	5 cut(s)	186, 231, 288, 1014, 1295
TspGWI	ACGGA	1 cut(s)	439
TspRI	CASTG	5 cut(s)	607, 760, 833, 1267, 1325
Tth111I	GACNNNGTC	2 cut(s)	344, 559
VpaK11BI	GGWCC	3 cut(s)	692, 794, 1048
XapI	RAATTY	4 cut(s)	51, 451, 468, 934
XceI	RCATGY	2 cut(s)	972, 1269
XmnI	GAANNNNTTC	2 cut(s)	177, 222
XspI	CTAG	2 cut(s)	1284, 1299
ZrmI	AGTACT	1 cut(s)	1015
Zsp2I	ATGCAT	1 cut(s)	1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.