MD03G1090000.v1.1

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009849
Physical Location & Seq
Reverse (-)
7419069 .. 7425067
5999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1090000.v1.1.491

Sequence Viewer

Length: 1371 bp
ATGGGTAGCCCAGAGAAGGATCAGAGCAAGGGCTTCTTCGCAGCCATGAGTTCTGGCCTCACAATGTTCGGCAACGCCATGCACCGATCCGTTGGCGGGTTGCTTGGTTATGAAGGGGTGGAAGTCATCAATCCAGAGGGAGGTGAAGAAGATGCAGAGGAGGAAGCTCAAAGAGGAAGATGGAAGGAGGAGGAACGAGATAGTTACTGGAGGATGATGCAGAAGTATATTGGCTCAGATGTGACATCAATGGTGACTCTTCCTGTACTTATTTTTGAGCCAATGACAATGTTGCAGAAGATGGCTGAGTTAATGGAGTATTCACACTTGTTGGATCACGCTGATGAATGTGAGGATCCTTATATGCAGCTGGTGTTTGCCACATCATGGGCTATATCAGTCTACTATGCCTACCAGCGGACATGGAAGCCATTCAACCCTATCCTTGGGGAGACTTATGAAATGGTTAATCACTTAGGGGTAACATTTATTGCAGAGCAGGTGAGTCATCATCCACCAATGAGTGCTGGGCATGCAGAGAATGAGCATTTCACTTATGATGTGACTTCAAAACTTAAAACTAAATTTTTAGGGAATTCTCTTGATGTTTACCCTGTTGGAAGAACACGTGTGACTCTTAAAAGAAATGGTGTCGTCTTAGATTTAGTGCCACCTCCCACAAAGGTAAACAACTTGATATTTGGCCGGACATGGGTTGATTCACCGGGGGAGATGGTCATGACCAATCTGACGACTGGGGACAAAGTTGTGATATATTTTCAGCCATGTGGCTGGTTTGGAGCTGGCCGCTATGAAGTGGATGGATATGTTTATAATGCTGCAGAGGAACCTCAGATATTGATGACTGGGAAATGGAACGAGTCATTGAGTTATCAATCATGTGATTTGGAAGGGGAACCCCTTCCAGGCTCAGAATTAAAAGAGGTTTGGCATGTTGCCGATGTTCCAAAGAATGACAAATTTCAGTACACATATTTTGCACATAAAATAAATAGCTTTGACACTGCTCCTAAAAAGTTGTTGGCATCAGACTCTCGTTTGCGGCCTGATAGATATGCACTTGAGAAGGGGGATCTGTCCAAATCTGGTGCAGAAAAGAGCAGATTGGAGGAGAGGCAGAGAGCTGAAAAGAGAGAGCGAGAGGCCAAGCAGCATCAGTTTAGGCCAAGATGGTTTGACTTGACAGAGGAAGTGACACCTACACCTTGGGGTGACTTGGAAGTCTATCAATATAACAATAAATACACTGAACACCGAGCCACAATTGATAGCTCAGACTCCATTGATACGGTTGATGTTACATCAATTGAATTCAACCCATGGCAGTATGGTAACCTGTCTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

457

Amino Acids

52.19

Weight (kDa)

5.02

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 68 - 426 1.3e-112 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AarI CACCTGC 1 cut(s) 490
AasI GACNNNNNNGTC 1 cut(s) 1241
Acc36I ACCTGC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 387
AccI GTMKAC 1 cut(s) 402
AciI CCGC 4 cut(s) 96, 418, 808, 1063
AclWI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
AcoI YGGCCR 2 cut(s) 703, 805
AcsI RAATTY 4 cut(s) 584, 595, 980, 1331
AcvI CACGTG 1 cut(s) 629
AfaI GTAC 2 cut(s) 267, 989
AfiI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
AflIII ACRYGT 2 cut(s) 626, 628
AgsI TTSAA 4 cut(s) 436, 570, 1331, 1336
AjnI CCWGG 1 cut(s) 925
AluBI AGCT 6 cut(s) 167, 370, 803, 1017, 1145, 1293
AluI AGCT 6 cut(s) 167, 370, 803, 1017, 1145, 1293
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
AoxI GGCC 6 cut(s) 55, 703, 805, 1064, 1164, 1184
ApeKI GCWGC 4 cut(s) 41, 367, 839, 1171
ApoI RAATTY 4 cut(s) 584, 595, 980, 1331
Asp700I GAANNNNTTC 2 cut(s) 431, 921
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 5 cut(s) 155, 265, 514, 714, 1244
BamHI GGATCC 1 cut(s) 355
BbrPI CACGTG 1 cut(s) 629
BbvI GCAGC 4 cut(s) 53, 379, 826, 1183
BccI CCATC 5 cut(s) 174, 295, 727, 815, 1185
BciT130I CCWGG 1 cut(s) 927
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 840, 1362
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 6 cut(s) 42, 368, 808, 840, 1064, 1172
BlsI GCNGC 6 cut(s) 43, 369, 809, 841, 1065, 1173
Bme1390I CCNGG 2 cut(s) 726, 927
BmiI GGNNCC 3 cut(s) 357, 849, 918
BmrFI CCNGG 2 cut(s) 726, 927
BmrI ACTGGG 2 cut(s) 765, 876
BmsI GCATC 4 cut(s) 142, 207, 1055, 1183
BmuI ACTGGG 2 cut(s) 765, 876
BpmI CTGGAG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 1 cut(s) 726
BsaAI YACGTR 1 cut(s) 629
BsaJI CCNNGG 4 cut(s) 445, 725, 1226, 1340
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
Bse1I ACTGG 3 cut(s) 212, 760, 871
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BseGI GGATG 3 cut(s) 219, 511, 826
BseLI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BseMII CTCAG 5 cut(s) 249, 297, 866, 945, 1308
BseNI ACTGG 3 cut(s) 212, 760, 871
BseRI GAGGAG 3 cut(s) 173, 203, 1145
BseXI GCAGC 4 cut(s) 53, 379, 826, 1183
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 1131
BshFI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BsiSI CCGG 2 cut(s) 706, 725
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
Bsp143I GATC 5 cut(s) 19, 86, 334, 355, 1093
Bsp19I CCATGG 1 cut(s) 1340
BspACI CCGC 4 cut(s) 96, 418, 808, 1063
BspANI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BspCNI CTCAG 5 cut(s) 248, 298, 865, 944, 1307
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 3 cut(s) 357, 849, 918
BspMAI CTGCAG 2 cut(s) 844, 1366
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
BsrI ACTGG 3 cut(s) 212, 760, 871
BssECI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BssMI GATC 5 cut(s) 19, 86, 334, 355, 1093
BssT1I CCWWGG 3 cut(s) 445, 1226, 1340
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 1 cut(s) 1312
Bst6I CTCTTC 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 534, 805
BstDEI CTNAG 7 cut(s) 235, 306, 475, 658, 852, 931, 1294
BstDSI CCRYGG 1 cut(s) 1340
BstEII GGTNACC 1 cut(s) 1352
BstF5I GGATG 3 cut(s) 219, 511, 826
BstKTI GATC 5 cut(s) 22, 89, 337, 358, 1096
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 5 cut(s) 19, 86, 334, 355, 1093
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 1 cut(s) 927
BstNSI RCATGY 2 cut(s) 536, 956
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 2 cut(s) 724, 925
BstSFI CTRYAG 2 cut(s) 840, 1362
BstV1I GCAGC 4 cut(s) 53, 379, 826, 1183
BstX2I RGATCY 2 cut(s) 355, 1093
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 2 cut(s) 355, 1093
BsuRI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BtgI CCRYGG 1 cut(s) 1340
BtsCI GGATG 3 cut(s) 219, 511, 826
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 1023, 1266
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 534, 805
CciI TCATGA 1 cut(s) 738
Csp6I GTAC 2 cut(s) 266, 988
CviQI GTAC 2 cut(s) 266, 988
DdeI CTNAG 7 cut(s) 235, 306, 475, 658, 852, 931, 1294
DpnI GATC 5 cut(s) 21, 88, 336, 357, 1095
DpnII GATC 5 cut(s) 19, 86, 334, 355, 1093
DrdI GACNNNNNNGTC 1 cut(s) 1241
DseDI GACNNNNNNGTC 1 cut(s) 1241
EaeI YGGCCR 2 cut(s) 703, 805
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 445, 1226, 1340
Eco72I CACGTG 1 cut(s) 629
Eco91I GGTNACC 1 cut(s) 1352
EcoO65I GGTNACC 1 cut(s) 1352
EcoRI GAATTC 2 cut(s) 595, 1331
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 445, 1226, 1340
ErhI CCWWGG 3 cut(s) 445, 1226, 1340
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 1 cut(s) 773
FauI CCCGC 1 cut(s) 89
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 6 cut(s) 42, 368, 808, 840, 1064, 1172
FokI GGATG 3 cut(s) 226, 498, 833
Fsp4HI GCNGC 6 cut(s) 42, 368, 808, 840, 1064, 1172
GluI GCNGC 6 cut(s) 42, 368, 808, 840, 1064, 1172
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
HapII CCGG 2 cut(s) 706, 725
HinfI GANTC 7 cut(s) 256, 505, 634, 719, 881, 1052, 1298
HpaII CCGG 2 cut(s) 706, 725
HphI GGTGA 5 cut(s) 155, 265, 514, 714, 1244
Hpy166II GTNNAC 4 cut(s) 403, 610, 688, 990
Hpy188I TCNGA 7 cut(s) 24, 238, 750, 855, 934, 1051, 1297
Hpy188III TCNNGA 3 cut(s) 134, 602, 739
Hpy8I GTNNAC 4 cut(s) 403, 610, 688, 990
HpyAV CCTTC 6 cut(s) 10, 107, 178, 905, 932, 1081
HpyCH4III ACNGT 1 cut(s) 1312
HpyCH4IV ACGT 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 7 cut(s) 235, 306, 475, 658, 852, 931, 1294
HpySE526I ACGT 1 cut(s) 628
Kzo9I GATC 5 cut(s) 19, 86, 334, 355, 1093
LmnI GCTCC 2 cut(s) 800, 1033
Lsp1109I GCAGC 4 cut(s) 53, 379, 826, 1183
LweI GCATC 4 cut(s) 142, 207, 1055, 1183
MaeII ACGT 1 cut(s) 628
MalI GATC 5 cut(s) 21, 88, 336, 357, 1095
MboI GATC 5 cut(s) 19, 86, 334, 355, 1093
MboII GAAGA 7 cut(s) 28, 158, 161, 189, 251, 310, 633
MfeI CAATTG 2 cut(s) 1284, 1326
MflI RGATCY 2 cut(s) 355, 1093
MluCI AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
MlyI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
MmeI TCCRAC 2 cut(s) 312, 598
MroXI GAANNNNTTC 2 cut(s) 431, 921
MseI TTAA 5 cut(s) 311, 468, 576, 639, 938
MslI CAYNNNNRTG 1 cut(s) 342
MspA1I CMGCKG 2 cut(s) 370, 418
MspI CCGG 2 cut(s) 706, 725
MspR9I CCNGG 2 cut(s) 726, 927
MunI CAATTG 2 cut(s) 1284, 1326
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 726
NcoI CCATGG 1 cut(s) 1340
NdeII GATC 5 cut(s) 19, 86, 334, 355, 1093
NlaIV GGNNCC 3 cut(s) 357, 849, 918
NmuCI GTSAC 6 cut(s) 241, 253, 562, 631, 1213, 1232
NspI RCATGY 2 cut(s) 536, 956
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 1 cut(s) 738
PaqCI CACCTGC 1 cut(s) 490
PdmI GAANNNNTTC 2 cut(s) 431, 921
PfeI GAWTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 6 cut(s) 43, 369, 809, 841, 1065, 1173
PleI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
PpsI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
Ppu21I YACGTR 1 cut(s) 629
PsiI TTATAA 1 cut(s) 834
Psp6I CCWGG 1 cut(s) 925
PspCI CACGTG 1 cut(s) 629
PspEI GGTNACC 1 cut(s) 1352
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 3 cut(s) 357, 849, 918
PstI CTGCAG 2 cut(s) 844, 1366
PsuI RGATCY 2 cut(s) 355, 1093
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 2 cut(s) 267, 989
RsaNI GTAC 2 cut(s) 266, 988
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 5 cut(s) 311, 468, 576, 639, 938
SatI GCNGC 6 cut(s) 42, 368, 808, 840, 1064, 1172
Sau3AI GATC 5 cut(s) 19, 86, 334, 355, 1093
SchI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
ScrFI CCNGG 2 cut(s) 726, 927
SfaNI GCATC 4 cut(s) 142, 207, 1055, 1183
SfcI CTRYAG 2 cut(s) 840, 1362
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SphI GCATGC 1 cut(s) 536
Sse9I AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
SsiI CCGC 4 cut(s) 96, 418, 808, 1063
StyD4I CCNGG 2 cut(s) 724, 925
StyI CCWWGG 3 cut(s) 445, 1226, 1340
TaaI ACNGT 1 cut(s) 1312
TaiI ACGT 1 cut(s) 631
TasI AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
TatI WGTACW 2 cut(s) 265, 987
TauI GCSGC 2 cut(s) 810, 1066
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 5 cut(s) 311, 468, 576, 639, 938
Tru9I TTAA 5 cut(s) 311, 468, 576, 639, 938
TscAI CASTG 2 cut(s) 1030, 1273
TseFI GTSAC 6 cut(s) 241, 253, 562, 631, 1213, 1232
TseI GCWGC 4 cut(s) 41, 367, 839, 1171
Tsp45I GTSAC 6 cut(s) 241, 253, 562, 631, 1213, 1232
TspDTI ATGAA 4 cut(s) 126, 360, 474, 828
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 1030, 1273
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 4 cut(s) 584, 595, 980, 1331
XceI RCATGY 2 cut(s) 536, 956
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 431, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.