pycom03g07160

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009849
Physical Location & Seq
Reverse (-)
5718912 .. 5724031
5120 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07160.1

Sequence Viewer

Length: 1371 bp
ATGGGTAGCCCAGAGAAGGATCAGAGCAAGGGCTTTTTCGCAGCCATGAGTTCTGGCCTCTCCATGTTCGGCAACGTCATGCACCGATCCGTCGGCGGGTTGCTTGGTTATGAAGGGGTGGAAGTCATCAATCCAGAGGGAGGCAAAGAAGATGCAGAGGAGGAAGCTCAAAGAGGAAGATGGAAGCAGGAGGAACGAGATAGTTACTGGAGGATGATGCAGAAGTATATTGGCTCAGATGTGACATCAATGGTGACTCTTCGTGTACTTATTTTTGAGCCGATGACAATGTTGCAGAAGATGGCTGAGTTAATGGAGTATTCATACTTGTTGGATCGCGCTGATGAATGTGAGGATCCTTATATGCAGTTGGCGTTTGCCGCATCATGGGCTATATCGGTCTACTATGCCTACCAGCGGACATGGAAGCCGTTCAACCCTATCCTTGGGGAGACTTATGAAATGGTTAATCACTCAGGGGTAACATTTATTGCAGAGCAGGTGAGTCATCATCCACCAATGAGTGCTGGGCATGCAGAGAATGAGCATTTCACTTATGATGTGACTTCAAAACTTAAAACTAAATTTTTAGGGAATTCGCTTGATGTTTACCCTGTTGGAAGAACACGTGTGACTCTTAAAAGAAATGGTGTTGTCTTAGGTTTAGTGCCGCCTCCCACAAAGGTAAACAACTTGATATTTGGCCGGACGTGGGTTGATTCACCAGGGGAGATGGTCATGACCAATCTGACGACTGGGGACAAAGTTGTGATATATTTTCAGCCATGTGGCTGGTTTGGAGCTGGCCGCTATGAAGTGGATGGATATGTTTATAATGCTGCAGAGGAACCTCAGATATTGATGACTGGGAAATGGAACGAGTCGTTGAGTTATCAACCATGTGATTTGGAAGGGGAACCCCTTCCAGGCACAGAATTAAAAGAGGTTTGGCATGTTGCCGATGTTCCAAAGAATGACAAATTTCAGTACACATATTTTGCACATAAAATAAATAGCTTTGACACTGCTCCTAAAAAGTTGTTGGCATCAGACTCTCGTTTGCGGCCTGATAGATATGCACTTGAGAAGGGGGATCTGTCCAAATCCGGTGCAGAAAAGGGCAGATTGGAGGAGAGGCAGAGAGCTGAAAAGAGAGAGCGAGAGGCCAAGCAGCATCAGTTTAGGCCAAGATGGTTTGACTTGACAGAGGAAGTTACACCTACACCTTGGGGTGACTTGGAAGTCTATCAATATAACAATAAATACACTGAATACCGAGCCTCAATTGATAGCTCTGACTCCATTGATACGGTTGATGTTACATCAATTGAATTCAACCCATGGCAGTATGGTAACCTGTCTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

457

Amino Acids

52.17

Weight (kDa)

5.15

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 68 - 426 2.6e-109 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AarI CACCTGC 1 cut(s) 490
AasI GACNNNNNNGTC 1 cut(s) 1241
Acc36I ACCTGC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 339
AciI CCGC 6 cut(s) 96, 381, 418, 671, 808, 1063
AclWI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
AcoI YGGCCR 2 cut(s) 703, 805
AcsI RAATTY 4 cut(s) 584, 595, 980, 1331
AcvI CACGTG 1 cut(s) 629
AfaI GTAC 2 cut(s) 267, 989
AfiI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
AflIII ACRYGT 2 cut(s) 626, 628
AgsI TTSAA 4 cut(s) 436, 570, 1331, 1336
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 2 cut(s) 724, 925
AluBI AGCT 5 cut(s) 167, 803, 1017, 1145, 1293
AluI AGCT 5 cut(s) 167, 803, 1017, 1145, 1293
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
AoxI GGCC 6 cut(s) 55, 703, 805, 1064, 1164, 1184
ApeKI GCWGC 3 cut(s) 41, 839, 1171
ApoI RAATTY 4 cut(s) 584, 595, 980, 1331
Asp700I GAANNNNTTC 2 cut(s) 431, 921
AspLEI GCGC 1 cut(s) 341
AsuHPI GGTGA 4 cut(s) 265, 514, 714, 1244
BamHI GGATCC 1 cut(s) 355
BbrPI CACGTG 1 cut(s) 629
BbvI GCAGC 3 cut(s) 53, 826, 1183
BccI CCATC 5 cut(s) 174, 295, 727, 815, 1185
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 2 cut(s) 726, 927
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 840, 1362
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 7 cut(s) 42, 381, 671, 808, 840, 1064, 1172
BlsI GCNGC 7 cut(s) 43, 382, 672, 809, 841, 1065, 1173
Bme1390I CCNGG 2 cut(s) 726, 927
BmgBI CACGTC 1 cut(s) 711
BmiI GGNNCC 3 cut(s) 357, 849, 918
BmrFI CCNGG 2 cut(s) 726, 927
BmrI ACTGGG 2 cut(s) 765, 876
BmsI GCATC 5 cut(s) 142, 207, 392, 1055, 1183
BmuI ACTGGG 2 cut(s) 765, 876
BpmI CTGGAG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 1103
BsaAI YACGTR 1 cut(s) 629
BsaJI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BsaWI WCCGGW 1 cut(s) 1106
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
Bse1I ACTGG 3 cut(s) 212, 760, 871
BseBI CCWGG 2 cut(s) 726, 927
BseDI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BseGI GGATG 3 cut(s) 219, 511, 826
BseLI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BseMII CTCAG 4 cut(s) 249, 297, 489, 866
BseNI ACTGG 3 cut(s) 212, 760, 871
BseRI GAGGAG 2 cut(s) 173, 1145
BseXI GCAGC 3 cut(s) 53, 826, 1183
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 1131
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BsiSI CCGG 2 cut(s) 706, 1107
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
Bsp143I GATC 5 cut(s) 19, 86, 334, 355, 1093
Bsp19I CCATGG 1 cut(s) 1340
BspACI CCGC 6 cut(s) 96, 381, 418, 671, 808, 1063
BspANI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BspCNI CTCAG 4 cut(s) 248, 298, 488, 865
BspFNI CGCG 1 cut(s) 339
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 3 cut(s) 357, 849, 918
BspMAI CTGCAG 2 cut(s) 844, 1366
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 6 cut(s) 27, 81, 342, 350, 363, 1101
BsrI ACTGG 3 cut(s) 212, 760, 871
BssECI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BssMI GATC 5 cut(s) 19, 86, 334, 355, 1093
BssT1I CCWWGG 3 cut(s) 445, 1226, 1340
Bst2UI CCWGG 2 cut(s) 726, 927
Bst4CI ACNGT 1 cut(s) 1312
Bst6I CTCTTC 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 534, 805
BstDEI CTNAG 5 cut(s) 235, 306, 475, 658, 852
BstDSI CCRYGG 1 cut(s) 1340
BstEII GGTNACC 1 cut(s) 1352
BstF5I GGATG 3 cut(s) 219, 511, 826
BstFNI CGCG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 5 cut(s) 22, 89, 337, 358, 1096
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 5 cut(s) 19, 86, 334, 355, 1093
BstMWI GCNNNNNNNGC 3 cut(s) 380, 389, 533
BstNI CCWGG 2 cut(s) 726, 927
BstNSI RCATGY 2 cut(s) 536, 956
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 2 cut(s) 724, 925
BstSFI CTRYAG 2 cut(s) 840, 1362
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 3 cut(s) 53, 826, 1183
BstX2I RGATCY 2 cut(s) 355, 1093
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 2 cut(s) 355, 1093
BsuRI GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
BtgI CCRYGG 1 cut(s) 1340
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 3 cut(s) 219, 511, 826
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 1023, 1266
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 534, 805
CciI TCATGA 1 cut(s) 738
CfoI GCGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 266, 988
CviQI GTAC 2 cut(s) 266, 988
DdeI CTNAG 5 cut(s) 235, 306, 475, 658, 852
DpnI GATC 5 cut(s) 21, 88, 336, 357, 1095
DpnII GATC 5 cut(s) 19, 86, 334, 355, 1093
DrdI GACNNNNNNGTC 1 cut(s) 1241
DseDI GACNNNNNNGTC 1 cut(s) 1241
EaeI YGGCCR 2 cut(s) 703, 805
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 445, 1226, 1340
Eco72I CACGTG 1 cut(s) 629
Eco91I GGTNACC 1 cut(s) 1352
EcoO65I GGTNACC 1 cut(s) 1352
EcoRI GAATTC 2 cut(s) 595, 1331
EcoRII CCWGG 2 cut(s) 724, 925
EcoT14I CCWWGG 3 cut(s) 445, 1226, 1340
ErhI CCWWGG 3 cut(s) 445, 1226, 1340
FaqI GGGAC 1 cut(s) 773
FauI CCCGC 1 cut(s) 89
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 7 cut(s) 42, 381, 671, 808, 840, 1064, 1172
FokI GGATG 3 cut(s) 226, 498, 833
Fsp4HI GCNGC 7 cut(s) 42, 381, 671, 808, 840, 1064, 1172
GlaI GCGC 1 cut(s) 340
GluI GCNGC 7 cut(s) 42, 381, 671, 808, 840, 1064, 1172
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 6 cut(s) 57, 705, 807, 1066, 1166, 1186
HapII CCGG 2 cut(s) 706, 1107
HhaI GCGC 1 cut(s) 341
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HinfI GANTC 7 cut(s) 256, 505, 634, 719, 881, 1052, 1298
HpaII CCGG 2 cut(s) 706, 1107
HphI GGTGA 4 cut(s) 265, 514, 714, 1244
Hpy166II GTNNAC 5 cut(s) 266, 403, 610, 688, 990
Hpy188I TCNGA 6 cut(s) 24, 238, 750, 855, 1051, 1297
Hpy188III TCNNGA 2 cut(s) 134, 739
Hpy8I GTNNAC 5 cut(s) 266, 403, 610, 688, 990
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 5 cut(s) 10, 107, 905, 932, 1081
HpyCH4III ACNGT 1 cut(s) 1312
HpyCH4IV ACGT 3 cut(s) 75, 628, 710
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 389, 533
HpyF3I CTNAG 5 cut(s) 235, 306, 475, 658, 852
HpySE526I ACGT 3 cut(s) 75, 628, 710
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 5 cut(s) 19, 86, 334, 355, 1093
LmnI GCTCC 2 cut(s) 800, 1033
Lsp1109I GCAGC 3 cut(s) 53, 826, 1183
LweI GCATC 5 cut(s) 142, 207, 392, 1055, 1183
MaeII ACGT 3 cut(s) 75, 628, 710
MalI GATC 5 cut(s) 21, 88, 336, 357, 1095
MboI GATC 5 cut(s) 19, 86, 334, 355, 1093
MboII GAAGA 5 cut(s) 161, 189, 251, 310, 633
MfeI CAATTG 2 cut(s) 1284, 1326
MflI RGATCY 2 cut(s) 355, 1093
MluCI AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
MlyI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
MmeI TCCRAC 2 cut(s) 312, 598
MroXI GAANNNNTTC 2 cut(s) 431, 921
MseI TTAA 5 cut(s) 311, 468, 576, 639, 938
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 2 cut(s) 706, 1107
MspR9I CCNGG 2 cut(s) 726, 927
MunI CAATTG 2 cut(s) 1284, 1326
MvaI CCWGG 2 cut(s) 726, 927
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 3 cut(s) 380, 389, 533
NcoI CCATGG 1 cut(s) 1340
NdeII GATC 5 cut(s) 19, 86, 334, 355, 1093
NlaIV GGNNCC 3 cut(s) 357, 849, 918
NmuCI GTSAC 5 cut(s) 241, 253, 562, 631, 1232
NspI RCATGY 2 cut(s) 536, 956
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 1 cut(s) 738
PaqCI CACCTGC 1 cut(s) 490
PdmI GAANNNNTTC 2 cut(s) 431, 921
PfeI GAWTC 1 cut(s) 719
PkrI GCNGC 7 cut(s) 43, 382, 672, 809, 841, 1065, 1173
PleI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
PpsI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
Ppu21I YACGTR 1 cut(s) 629
PsiI TTATAA 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 724, 925
PspCI CACGTG 1 cut(s) 629
PspEI GGTNACC 1 cut(s) 1352
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 724, 925
PspN4I GGNNCC 3 cut(s) 357, 849, 918
PstI CTGCAG 2 cut(s) 844, 1366
PsuI RGATCY 2 cut(s) 355, 1093
RsaI GTAC 2 cut(s) 267, 989
RsaNI GTAC 2 cut(s) 266, 988
SaqAI TTAA 5 cut(s) 311, 468, 576, 639, 938
SatI GCNGC 7 cut(s) 42, 381, 671, 808, 840, 1064, 1172
Sau3AI GATC 5 cut(s) 19, 86, 334, 355, 1093
SchI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
ScrFI CCNGG 2 cut(s) 726, 927
SfaNI GCATC 5 cut(s) 142, 207, 392, 1055, 1183
SfcI CTRYAG 2 cut(s) 840, 1362
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SphI GCATGC 1 cut(s) 536
Sse9I AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
SsiI CCGC 6 cut(s) 96, 381, 418, 671, 808, 1063
StyD4I CCNGG 2 cut(s) 724, 925
StyI CCWWGG 3 cut(s) 445, 1226, 1340
TaaI ACNGT 1 cut(s) 1312
TaiI ACGT 3 cut(s) 78, 631, 713
TaqII GACCGA 1 cut(s) 388
TasI AATT 7 cut(s) 584, 595, 935, 980, 1284, 1326, 1331
TatI WGTACW 2 cut(s) 265, 987
TauI GCSGC 4 cut(s) 383, 673, 810, 1066
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 5 cut(s) 311, 468, 576, 639, 938
Tru9I TTAA 5 cut(s) 311, 468, 576, 639, 938
TscAI CASTG 2 cut(s) 1030, 1273
TseFI GTSAC 5 cut(s) 241, 253, 562, 631, 1232
TseI GCWGC 3 cut(s) 41, 839, 1171
Tsp45I GTSAC 5 cut(s) 241, 253, 562, 631, 1232
TspDTI ATGAA 5 cut(s) 126, 312, 360, 474, 828
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 1030, 1273
XapI RAATTY 4 cut(s) 584, 595, 980, 1331
XceI RCATGY 2 cut(s) 536, 956
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 431, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.