Rroxscaffold_1G00015830

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009849
Physical Location & Seq
Reverse (-)
19717135 .. 19722309
5175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00015830.1

Sequence Viewer

Length: 1377 bp
ATGAGTATCCCAAACAAGGATGAGAAGAACAACAAGGGCTTCTTCGCCGCCATGAGTTCCGGCCTCTCCGTCTTCGGCAGCGCCATGCACCGATCCGTTGGCGGGATGCTTGGTTATGAAGGGGTGGAAGTTATAAATCCAGAGGGAGGCAAAGAAGACGCAGAGGAGGAAGCTCAACGAGGAAGATGGAAGCAGGAGGAACGAGACAGTTACTGGAAGATGATGCAAAAGTATATAGGTTCAGATATAACATCAATGGTGACTCTTCCTGTATATATTTTTGAGCCAATGACAATGCTGCAGAAAATGGCAGAGCTGATGGAATATTCACACTTGTTGGATCGTGCTGATGAATGTGAGGATCCTTATATGCGGTTGGTGTACTCCACTTCATGGGCTATATCTGTCTACTACGCCTACCAGCGAACATGGAAACCATTTAATCCTATACTTGGGGAGACTTATGAGATGGTTAATCACTGTGGGATTACATTCGTTGCAGAGCAGGTCAGTCATCATCCACCAATGAGTGCTGCTCATGGTGAAAATGAGCATTTCACTTATGATGTGACGTCAAAGCTGAAAACTAAATTTTTAGGGAACTCTCTTGATGTTTACCCTGTTGGAAGAACCCGAGTGACTCTTAAAAGAGATGGTGTAGTCTTAGATTTAGTGCCACCTCCCACAAAGGTAAACAACTTGATATTCGGACGGACTTGGGTTGATTCACCTGGAGAGATGATCATGACAAATCTGACAACTGGGGACAAAGCTGTGCTATGTTTCCAGCCTTGTGGCTGGTTTGGAGCTGGTCGTTATGAAGTGGATGGATATGTTTATAATTCTGCTGAGGAACCTAAGATATTGATGACTGGGAAATGGAATGAATCCATGAATTATCAACCTTGTGACATGGAAGGGGAACCCCTTCCAGGAACAGAATTAAAAGAGGTTTGGCATATTGCCGATGTTCCGGAGAATGACAAATTTCAGTACACATACTTTGCACATAAAATAAATAACTTTAACACTGCTCCTAAAAAGTTGTTGGCATCGGACTCGCGTTTGCGCCCTGATAGATATGCACTTGAGAACGGCGATCTATCCAAAGCAGGTGCAGAGAAGAGCAGATTGGAGGAGAGGCAGAGAGCTGAAAAGAGAGAACGAGAAGCAAAGGAACAAAAGTTTACACCAAGATGGTTCGACTTAACTGAGGAGGTTACACCTACACCGTGGGGTGACTTGGAAGTCTATCAATATAACAGTAAATACACTCAACACCGTGCTGCAATTGATAGCTCTGACTCTGTTGATGGGGTTGATGTGAAATCAATTGACTTCAACCCATGGCAGTATGGCAATGTGTCCGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

458

Amino Acids

52.41

Weight (kDa)

5.15

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 70 - 428 2.8e-114 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 134, 840
AarI CACCTGC 1 cut(s) 1103
AasI GACNNNNNNGTC 1 cut(s) 1247
AatII GACGTC 1 cut(s) 575
Acc36I ACCTGC 2 cut(s) 496, 1103
AccB7I CCANNNNNTGG 1 cut(s) 393
AccI GTMKAC 1 cut(s) 408
AccII CGCG 1 cut(s) 1063
AccIII TCCGGA 1 cut(s) 973
AciI CCGC 4 cut(s) 48, 102, 373, 1368
AclWI GGATC 4 cut(s) 87, 348, 356, 369
AcsI RAATTY 2 cut(s) 590, 986
AcyI GRCGYC 1 cut(s) 572
AfaI GTAC 2 cut(s) 383, 995
AfiI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 393, 452, 932
AgsI TTSAA 1 cut(s) 1342
AjnI CCWGG 2 cut(s) 730, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
AluBI AGCT 7 cut(s) 173, 316, 580, 773, 809, 1151, 1299
AluI AGCT 7 cut(s) 173, 316, 580, 773, 809, 1151, 1299
Alw26I GTCTC 2 cut(s) 198, 452
AlwI GGATC 4 cut(s) 87, 348, 356, 369
AlwNI CAGNNNCTG 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 633
Aor13HI TCCGGA 1 cut(s) 973
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 4 cut(s) 78, 298, 533, 1286
ApoI RAATTY 2 cut(s) 590, 986
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 2 cut(s) 83, 1071
AsuHPI GGTGA 4 cut(s) 271, 554, 720, 1250
AvaI CYCGRG 1 cut(s) 633
BamHI GGATCC 1 cut(s) 361
BbsI GAAGAC 2 cut(s) 64, 162
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 4 cut(s) 90, 285, 520, 1273
BccI CCATC 7 cut(s) 180, 313, 463, 647, 821, 1191, 1307
BceAI ACGGC 1 cut(s) 1111
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 2 cut(s) 732, 933
BciVI GTATCC 1 cut(s) 17
BclI TGATCA 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 198, 452
BfmI CTRYAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 2 cut(s) 496, 1103
BfuI GTATCC 1 cut(s) 17
BisI GCNGC 5 cut(s) 48, 79, 299, 534, 1287
BlsI GCNGC 5 cut(s) 49, 80, 300, 535, 1288
Bme1390I CCNGG 2 cut(s) 732, 933
BmeT110I CYCGRG 1 cut(s) 633
BmiI GGNNCC 3 cut(s) 363, 855, 924
BmrFI CCNGG 2 cut(s) 732, 933
BmrI ACTGGG 2 cut(s) 771, 882
BmsI GCATC 3 cut(s) 96, 213, 1061
BmuI ACTGGG 2 cut(s) 771, 882
BpiI GAAGAC 2 cut(s) 64, 162
BplI GAGNNNNNCTC 2 cut(s) 520, 552
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 849
BpuEI CTTGAG 1 cut(s) 1109
BsaHI GRCGYC 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 1232, 1346
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 102, 146, 393, 452, 932
Bse1I ACTGG 3 cut(s) 218, 766, 877
Bse3DI GCAATG 1 cut(s) 1366
BseAI TCCGGA 1 cut(s) 973
BseBI CCWGG 2 cut(s) 732, 933
BseDI CCNNGG 2 cut(s) 1232, 1346
BseGI GGATG 4 cut(s) 25, 111, 517, 832
BseLI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 393, 452, 932
BseMI GCAATG 1 cut(s) 1366
BseMII CTCAG 2 cut(s) 840, 1203
BseNI ACTGG 3 cut(s) 218, 766, 877
BseRI GAGGAG 3 cut(s) 179, 1151, 1229
BseXI GCAGC 4 cut(s) 90, 285, 520, 1273
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 2 cut(s) 60, 974
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 393, 452, 932
BsmAI GTCTC 2 cut(s) 198, 452
BsmFI GGGAC 1 cut(s) 779
BsnI GGCC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 633
Bsp13I TCCGGA 1 cut(s) 973
Bsp143I GATC 5 cut(s) 92, 340, 361, 741, 1099
Bsp19I CCATGG 1 cut(s) 1346
BspACI CCGC 4 cut(s) 48, 102, 373, 1368
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 2 cut(s) 841, 1204
BspEI TCCGGA 1 cut(s) 973
BspFNI CGCG 1 cut(s) 1063
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 363, 855, 924
BspMAI CTGCAG 1 cut(s) 303
BspMI ACCTGC 2 cut(s) 496, 1103
BspPI GGATC 4 cut(s) 87, 348, 356, 369
BspQI GCTCTTC 1 cut(s) 1118
BsrDI GCAATG 1 cut(s) 1366
BsrI ACTGG 3 cut(s) 218, 766, 877
BssECI CCNNGG 2 cut(s) 1232, 1346
BssMI GATC 5 cut(s) 92, 340, 361, 741, 1099
BssNI GRCGYC 1 cut(s) 572
BssT1I CCWWGG 1 cut(s) 1346
Bst2UI CCWGG 2 cut(s) 732, 933
Bst4CI ACNGT 5 cut(s) 209, 482, 1233, 1265, 1283
Bst6I CTCTTC 2 cut(s) 270, 1118
BstACI GRCGYC 1 cut(s) 572
BstDEI CTNAG 4 cut(s) 664, 849, 858, 1212
BstDSI CCRYGG 2 cut(s) 1232, 1346
BstF5I GGATG 4 cut(s) 25, 111, 517, 832
BstFNI CGCG 1 cut(s) 1063
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 1071
BstKTI GATC 5 cut(s) 95, 343, 364, 744, 1102
BstMAI GTCTC 2 cut(s) 198, 452
BstMBI GATC 5 cut(s) 92, 340, 361, 741, 1099
BstNI CCWGG 2 cut(s) 732, 933
BstSCI CCNGG 2 cut(s) 730, 931
BstSFI CTRYAG 1 cut(s) 299
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 4 cut(s) 90, 285, 520, 1273
BstV2I GAAGAC 2 cut(s) 64, 162
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 794
BstYI RGATCY 1 cut(s) 361
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 2 cut(s) 1232, 1346
BtsCI GGATG 4 cut(s) 25, 111, 517, 832
BtsI GCAGTG 1 cut(s) 1029
BtsIMutI CAGTG 2 cut(s) 478, 1029
BveI ACCTGC 2 cut(s) 496, 1103
CaiI CAGNNNCTG 1 cut(s) 213
CciI TCATGA 1 cut(s) 744
CfoI GCGC 2 cut(s) 83, 1071
CseI GACGC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 382, 994
CviAII CATG 9 cut(s) 52, 85, 393, 429, 539, 745, 892, 913, 1347
CviQI GTAC 2 cut(s) 382, 994
DdeI CTNAG 4 cut(s) 664, 849, 858, 1212
DpnI GATC 5 cut(s) 94, 342, 363, 743, 1101
DpnII GATC 5 cut(s) 92, 340, 361, 741, 1099
DrdI GACNNNNNNGTC 1 cut(s) 1247
DseDI GACNNNNNNGTC 1 cut(s) 1247
Eam1104I CTCTTC 2 cut(s) 270, 1118
EarI CTCTTC 2 cut(s) 270, 1118
Eco130I CCWWGG 1 cut(s) 1346
Eco88I CYCGRG 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 730, 931
EcoT14I CCWWGG 1 cut(s) 1346
ErhI CCWWGG 1 cut(s) 1346
FaeI CATG 9 cut(s) 55, 88, 396, 432, 542, 748, 895, 916, 1350
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 779
FatI CATG 9 cut(s) 51, 84, 392, 428, 538, 744, 891, 912, 1346
FauI CCCGC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 5 cut(s) 48, 79, 299, 534, 1287
FokI GGATG 4 cut(s) 32, 118, 504, 839
Fsp4HI GCNGC 5 cut(s) 48, 79, 299, 534, 1287
GlaI GCGC 2 cut(s) 82, 1070
GluI GCNGC 5 cut(s) 48, 79, 299, 534, 1287
GsuI CTGGAG 1 cut(s) 753
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 60, 974
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 83, 1071
Hin1I GRCGYC 1 cut(s) 572
Hin1II CATG 9 cut(s) 55, 88, 396, 432, 542, 748, 895, 916, 1350
Hin6I GCGC 2 cut(s) 81, 1069
HinP1I GCGC 2 cut(s) 81, 1069
HinfI GANTC 6 cut(s) 262, 640, 725, 887, 1058, 1304
HpaII CCGG 2 cut(s) 60, 974
HphI GGTGA 4 cut(s) 271, 554, 720, 1250
Hpy166II GTNNAC 6 cut(s) 382, 409, 616, 694, 996, 1188
Hpy188I TCNGA 5 cut(s) 244, 710, 756, 1057, 1303
Hpy188III TCNNGA 4 cut(s) 140, 608, 745, 974
Hpy8I GTNNAC 6 cut(s) 382, 409, 616, 694, 996, 1188
HpyAV CCTTC 3 cut(s) 113, 911, 938
HpyCH4III ACNGT 5 cut(s) 209, 482, 1233, 1265, 1283
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 8 cut(s) 88, 226, 301, 500, 1007, 1085, 1118, 1289
HpyF3I CTNAG 4 cut(s) 664, 849, 858, 1212
HpySE526I ACGT 1 cut(s) 572
Hsp92I GRCGYC 1 cut(s) 572
Hsp92II CATG 9 cut(s) 55, 88, 396, 432, 542, 748, 895, 916, 1350
HspAI GCGC 2 cut(s) 81, 1069
Kpn2I TCCGGA 1 cut(s) 973
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 5 cut(s) 92, 340, 361, 741, 1099
LguI GCTCTTC 1 cut(s) 1118
LmnI GCTCC 2 cut(s) 806, 1039
Lsp1109I GCAGC 4 cut(s) 90, 285, 520, 1273
LweI GCATC 3 cut(s) 96, 213, 1061
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 7 cut(s) 209, 259, 568, 637, 908, 1219, 1238
MalI GATC 5 cut(s) 94, 342, 363, 743, 1101
MboI GATC 5 cut(s) 92, 340, 361, 741, 1099
MboII GAAGA 9 cut(s) 34, 37, 64, 167, 195, 229, 257, 639, 1135
MfeI CAATTG 2 cut(s) 1290, 1332
MflI RGATCY 1 cut(s) 361
MluCI AATT 7 cut(s) 590, 841, 895, 941, 986, 1290, 1332
MlyI GAGTC 4 cut(s) 256, 634, 1052, 1298
MmeI TCCRAC 2 cut(s) 318, 604
MroI TCCGGA 1 cut(s) 973
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 6 cut(s) 441, 474, 645, 944, 1026, 1208
MslI CAYNNNNRTG 1 cut(s) 1195
MspI CCGG 2 cut(s) 60, 974
MspR9I CCNGG 2 cut(s) 732, 933
MunI CAATTG 2 cut(s) 1290, 1332
MvaI CCWGG 2 cut(s) 732, 933
MvnI CGCG 1 cut(s) 1063
NcoI CCATGG 1 cut(s) 1346
NdeII GATC 5 cut(s) 92, 340, 361, 741, 1099
NlaIII CATG 9 cut(s) 55, 88, 396, 432, 542, 748, 895, 916, 1350
NlaIV GGNNCC 3 cut(s) 363, 855, 924
NmuCI GTSAC 5 cut(s) 259, 568, 637, 908, 1238
PagI TCATGA 1 cut(s) 744
PaqCI CACCTGC 1 cut(s) 1103
PciSI GCTCTTC 1 cut(s) 1118
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 2 cut(s) 725, 887
PflMI CCANNNNNTGG 1 cut(s) 393
PfoI TCCNGGA 1 cut(s) 931
PkrI GCNGC 5 cut(s) 49, 80, 300, 535, 1288
PleI GAGTC 4 cut(s) 256, 634, 1052, 1298
PpsI GAGTC 4 cut(s) 256, 634, 1052, 1298
PsiI TTATAA 2 cut(s) 134, 840
Psp6I CCWGG 2 cut(s) 730, 931
PspGI CCWGG 2 cut(s) 730, 931
PspN4I GGNNCC 3 cut(s) 363, 855, 924
PstI CTGCAG 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 213
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 2 cut(s) 383, 995
RsaNI GTAC 2 cut(s) 382, 994
RseI CAYNNNNRTG 1 cut(s) 1195
SapI GCTCTTC 1 cut(s) 1118
SaqAI TTAA 6 cut(s) 441, 474, 645, 944, 1026, 1208
SatI GCNGC 5 cut(s) 48, 79, 299, 534, 1287
Sau3AI GATC 5 cut(s) 92, 340, 361, 741, 1099
SchI GAGTC 4 cut(s) 256, 634, 1052, 1298
ScrFI CCNGG 2 cut(s) 732, 933
SfaNI GCATC 3 cut(s) 96, 213, 1061
SfcI CTRYAG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 1195
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
Sse9I AATT 7 cut(s) 590, 841, 895, 941, 986, 1290, 1332
SsiI CCGC 4 cut(s) 48, 102, 373, 1368
SspI AATATT 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 730, 931
StyI CCWWGG 1 cut(s) 1346
TaaI ACNGT 5 cut(s) 209, 482, 1233, 1265, 1283
TaiI ACGT 1 cut(s) 575
TaqI TCGA 1 cut(s) 1203
TasI AATT 7 cut(s) 590, 841, 895, 941, 986, 1290, 1332
TatI WGTACW 2 cut(s) 381, 993
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 725, 887
Tru1I TTAA 6 cut(s) 441, 474, 645, 944, 1026, 1208
Tru9I TTAA 6 cut(s) 441, 474, 645, 944, 1026, 1208
TscAI CASTG 2 cut(s) 485, 1036
TseFI GTSAC 5 cut(s) 259, 568, 637, 908, 1238
TseI GCWGC 4 cut(s) 78, 298, 533, 1286
Tsp45I GTSAC 5 cut(s) 259, 568, 637, 908, 1238
TspDTI ATGAA 6 cut(s) 132, 366, 381, 834, 900, 908
TspGWI ACGGA 3 cut(s) 58, 85, 727
TspRI CASTG 2 cut(s) 485, 1036
Van91I CCANNNNNTGG 1 cut(s) 393
XapI RAATTY 2 cut(s) 590, 986
XmiI GTMKAC 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 927
ZraI GACGTC 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.