Rh5BG444700

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009849
Physical Location & Seq
Forward (+)
71357503 .. 71362684
5182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG444700.1

Sequence Viewer

Length: 1272 bp
ATGCTTGGTTATGAAGGGGTGGAAGTTATAAATCCAGAGGGAGGCAAAGAAGACGCAGAGGAGGAAGCTCAACGAGGAAGATGGAAACAGGAGGAACGAGACAGTTACTGGAAGATGATGCAAAAGTATATAGGTTCAGATATAACATCAATGGTGACTCTTCCTGTATATATTTTTGAGCCAATGACAATGCTGCAGAAAATGGCAGAGCTTATGGAATATTCACACTTGTTGGATCGCGCTGATGAATGTGAGGATCCTTATATGCGGTTGGTGTACACCACTTCATGGGCTATATCTGTCTACTACGCCTACCAGCGAACATGGAAACCATTTAATCCTATACTTGGGGAGACTTATGAGATGGTTAATCACTGTGGGATTACATTCGTTGCAGAGCAGGTTAGTCATCATCCACCAATGAGTGCTGCTCATGGTGAAAATGAGCATTTCACTTATGATGTGACGTCAAAGCTGAAAACTAAATTTTTAGGAAACTCTCTTGATGTTTACCCTGTTGGAAGAACCCGAGTGACTCTTAAAAGAGATGGTGTAGTCTTAGATTTAGTGCCACCTCCCACAAAGGTAAACAACTTGATATTCGGACGGACTTGGGTTGATTCACCTGGAGAGATGATCATGACAAATCTGACAACTGGGGACAAAGCTGTGCTATGTTTCCAGCCTTGTGGCTGGTTTGGAGCTGGTCGTTATGAAGTGGATGGATATGTTTATAATTCTGCTGAGGAACCTAAGATATTGATGACTGGGAAATGGAATGAATCCATGAATTATCAACCTTGTGACATGGAAGGGGAACCCCTTCCAGGAACAGAATTAAAAGAGGTTTGGCATATTGCCGATGTTCCGGAGAATGACAAATTTCAGTACACATACTTTGCACATAAAATAAATAGCTTTAACACTGCTCCTAAAAAGTTGTTGGCATCGGACTCGCGTTTGCGCCCTGATAGATATGCACTTGAGAACGGCGATCTATCCAAAGCAGGTGCAGAGAAGAGCAGATTGGAGGAGAGGCAGAGAGCTGAAAAGAGAGAACGAGAAGCAAAGGAACAAAAGTTTACACCAAGATGGTTCGACTTAACTGAGGAGGTTACACCTACACCGTGGGGTGACTTGGAAGTCTATCAGTATAACAGTAAATATACTCAACACCGTGCTGCAATTGATAGCTCTGACTCTGTTGATGGGGTTGATGTGAAATCAATTGACTTCAACCCATGGCAGTATGGCAATGTGTCCGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

423

Amino Acids

48.76

Weight (kDa)

5.01

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 35 - 393 1e-114 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 29, 735
AarI CACCTGC 1 cut(s) 998
AasI GACNNNNNNGTC 1 cut(s) 1142
AatII GACGTC 1 cut(s) 470
Acc36I ACCTGC 2 cut(s) 391, 998
AccB7I CCANNNNNTGG 1 cut(s) 288
AccI GTMKAC 1 cut(s) 303
AccII CGCG 2 cut(s) 240, 958
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 2 cut(s) 268, 1263
AclWI GGATC 3 cut(s) 243, 251, 264
AcsI RAATTY 2 cut(s) 485, 881
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 2 cut(s) 278, 890
AfiI CCNNNNNNNGG 4 cut(s) 41, 288, 347, 827
AgsI TTSAA 1 cut(s) 1237
AjnI CCWGG 2 cut(s) 625, 826
AjuI GAANNNNNNNTTGG 2 cut(s) 1010, 1042
AluBI AGCT 8 cut(s) 68, 211, 475, 668, 704, 918, 1046, 1194
AluI AGCT 8 cut(s) 68, 211, 475, 668, 704, 918, 1046, 1194
Alw26I GTCTC 2 cut(s) 93, 347
AlwI GGATC 3 cut(s) 243, 251, 264
AlwNI CAGNNNCTG 1 cut(s) 108
Ama87I CYCGRG 1 cut(s) 528
Aor13HI TCCGGA 1 cut(s) 868
ApeKI GCWGC 3 cut(s) 193, 428, 1181
ApoI RAATTY 2 cut(s) 485, 881
Asp700I GAANNNNTTC 1 cut(s) 822
AspLEI GCGC 2 cut(s) 242, 966
AsuHPI GGTGA 4 cut(s) 166, 449, 615, 1145
AvaI CYCGRG 1 cut(s) 528
BamHI GGATCC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 57
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 3 cut(s) 180, 415, 1168
BccI CCATC 6 cut(s) 75, 358, 542, 716, 1086, 1202
BceAI ACGGC 1 cut(s) 1006
BcgI CGANNNNNNTGC 2 cut(s) 936, 970
BciT130I CCWGG 2 cut(s) 627, 828
BclI TGATCA 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 93, 347
BfmI CTRYAG 1 cut(s) 194
BfuAI ACCTGC 2 cut(s) 391, 998
BisI GCNGC 3 cut(s) 194, 429, 1182
BlsI GCNGC 3 cut(s) 195, 430, 1183
Bme1390I CCNGG 2 cut(s) 627, 828
BmeT110I CYCGRG 1 cut(s) 528
BmiI GGNNCC 3 cut(s) 258, 750, 819
BmrFI CCNGG 2 cut(s) 627, 828
BmrI ACTGGG 2 cut(s) 666, 777
BmsI GCATC 2 cut(s) 108, 956
BmuI ACTGGG 2 cut(s) 666, 777
BpiI GAAGAC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 415, 447
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 744
BpuEI CTTGAG 1 cut(s) 1004
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 1127, 1241
BsaWI WCCGGW 1 cut(s) 868
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 288, 347, 827
Bse1I ACTGG 3 cut(s) 113, 661, 772
Bse3DI GCAATG 1 cut(s) 1261
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 2 cut(s) 627, 828
BseDI CCNNGG 2 cut(s) 1127, 1241
BseGI GGATG 2 cut(s) 412, 727
BseLI CCNNNNNNNGG 4 cut(s) 41, 288, 347, 827
BseMI GCAATG 1 cut(s) 1261
BseMII CTCAG 2 cut(s) 735, 1098
BseNI ACTGG 3 cut(s) 113, 661, 772
BseRI GAGGAG 3 cut(s) 74, 1046, 1124
BseXI GCAGC 3 cut(s) 180, 415, 1168
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 2 cut(s) 240, 958
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 1 cut(s) 869
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 4 cut(s) 41, 288, 347, 827
BsmAI GTCTC 2 cut(s) 93, 347
BsmFI GGGAC 1 cut(s) 674
BsoBI CYCGRG 1 cut(s) 528
Bsp13I TCCGGA 1 cut(s) 868
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 4 cut(s) 235, 256, 636, 994
Bsp19I CCATGG 1 cut(s) 1241
BspACI CCGC 2 cut(s) 268, 1263
BspCNI CTCAG 2 cut(s) 736, 1099
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 2 cut(s) 240, 958
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 3 cut(s) 258, 750, 819
BspMAI CTGCAG 1 cut(s) 198
BspMI ACCTGC 2 cut(s) 391, 998
BspPI GGATC 3 cut(s) 243, 251, 264
BspQI GCTCTTC 1 cut(s) 1013
BsrDI GCAATG 1 cut(s) 1261
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 3 cut(s) 113, 661, 772
BssECI CCNNGG 2 cut(s) 1127, 1241
BssMI GATC 4 cut(s) 235, 256, 636, 994
BssNI GRCGYC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 1241
Bst2UI CCWGG 2 cut(s) 627, 828
Bst4CI ACNGT 5 cut(s) 104, 377, 1128, 1160, 1178
Bst6I CTCTTC 2 cut(s) 165, 1013
BstACI GRCGYC 1 cut(s) 467
BstAUI TGTACA 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 559, 744, 753, 1107
BstDSI CCRYGG 2 cut(s) 1127, 1241
BstF5I GGATG 2 cut(s) 412, 727
BstFNI CGCG 2 cut(s) 240, 958
BstHHI GCGC 2 cut(s) 242, 966
BstKTI GATC 4 cut(s) 238, 259, 639, 997
BstMAI GTCTC 2 cut(s) 93, 347
BstMBI GATC 4 cut(s) 235, 256, 636, 994
BstNI CCWGG 2 cut(s) 627, 828
BstSCI CCNGG 2 cut(s) 625, 826
BstSFI CTRYAG 1 cut(s) 194
BstUI CGCG 2 cut(s) 240, 958
BstV1I GCAGC 3 cut(s) 180, 415, 1168
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 689
BstYI RGATCY 1 cut(s) 256
BtgI CCRYGG 2 cut(s) 1127, 1241
BtsCI GGATG 2 cut(s) 412, 727
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 2 cut(s) 373, 924
BveI ACCTGC 2 cut(s) 391, 998
CaiI CAGNNNCTG 1 cut(s) 108
CciI TCATGA 1 cut(s) 639
CfoI GCGC 2 cut(s) 242, 966
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 277, 889
CviAII CATG 7 cut(s) 288, 324, 434, 640, 787, 808, 1242
CviQI GTAC 2 cut(s) 277, 889
DdeI CTNAG 4 cut(s) 559, 744, 753, 1107
DpnI GATC 4 cut(s) 237, 258, 638, 996
DpnII GATC 4 cut(s) 235, 256, 636, 994
DrdI GACNNNNNNGTC 1 cut(s) 1142
DseDI GACNNNNNNGTC 1 cut(s) 1142
Eam1104I CTCTTC 2 cut(s) 165, 1013
EarI CTCTTC 2 cut(s) 165, 1013
Eco130I CCWWGG 1 cut(s) 1241
Eco88I CYCGRG 1 cut(s) 528
EcoRII CCWGG 2 cut(s) 625, 826
EcoT14I CCWWGG 1 cut(s) 1241
ErhI CCWWGG 1 cut(s) 1241
FaeI CATG 7 cut(s) 291, 327, 437, 643, 790, 811, 1245
FaqI GGGAC 1 cut(s) 674
FatI CATG 7 cut(s) 287, 323, 433, 639, 786, 807, 1241
FbaI TGATCA 1 cut(s) 636
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 194, 429, 1182
FokI GGATG 2 cut(s) 399, 734
Fsp4HI GCNGC 3 cut(s) 194, 429, 1182
GlaI GCGC 2 cut(s) 241, 965
GluI GCNGC 3 cut(s) 194, 429, 1182
GsuI CTGGAG 1 cut(s) 648
HapII CCGG 1 cut(s) 869
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 242, 966
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 7 cut(s) 291, 327, 437, 643, 790, 811, 1245
Hin6I GCGC 2 cut(s) 240, 964
HinP1I GCGC 2 cut(s) 240, 964
HinfI GANTC 6 cut(s) 157, 535, 620, 782, 953, 1199
HpaII CCGG 1 cut(s) 869
HphI GGTGA 4 cut(s) 166, 449, 615, 1145
Hpy166II GTNNAC 7 cut(s) 277, 279, 304, 511, 589, 891, 1083
Hpy188I TCNGA 5 cut(s) 139, 605, 651, 952, 1198
Hpy188III TCNNGA 4 cut(s) 35, 503, 640, 869
Hpy8I GTNNAC 7 cut(s) 277, 279, 304, 511, 589, 891, 1083
HpyAV CCTTC 3 cut(s) 8, 806, 833
HpyCH4III ACNGT 5 cut(s) 104, 377, 1128, 1160, 1178
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 7 cut(s) 121, 196, 395, 902, 980, 1013, 1184
HpyF3I CTNAG 4 cut(s) 559, 744, 753, 1107
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 7 cut(s) 291, 327, 437, 643, 790, 811, 1245
HspAI GCGC 2 cut(s) 240, 964
Kpn2I TCCGGA 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 4 cut(s) 235, 256, 636, 994
LguI GCTCTTC 1 cut(s) 1013
LmnI GCTCC 2 cut(s) 701, 934
Lsp1109I GCAGC 3 cut(s) 180, 415, 1168
LweI GCATC 2 cut(s) 108, 956
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 7 cut(s) 104, 154, 463, 532, 803, 1114, 1133
MalI GATC 4 cut(s) 237, 258, 638, 996
MboI GATC 4 cut(s) 235, 256, 636, 994
MboII GAAGA 6 cut(s) 62, 90, 124, 152, 534, 1030
MfeI CAATTG 2 cut(s) 1185, 1227
MflI RGATCY 1 cut(s) 256
MluCI AATT 7 cut(s) 485, 736, 790, 836, 881, 1185, 1227
MlyI GAGTC 4 cut(s) 151, 529, 947, 1193
MmeI TCCRAC 2 cut(s) 213, 499
MroI TCCGGA 1 cut(s) 868
MroXI GAANNNNTTC 1 cut(s) 822
MseI TTAA 6 cut(s) 336, 369, 540, 839, 921, 1103
MslI CAYNNNNRTG 1 cut(s) 1090
MspI CCGG 1 cut(s) 869
MspR9I CCNGG 2 cut(s) 627, 828
MunI CAATTG 2 cut(s) 1185, 1227
MvaI CCWGG 2 cut(s) 627, 828
MvnI CGCG 2 cut(s) 240, 958
NcoI CCATGG 1 cut(s) 1241
NdeII GATC 4 cut(s) 235, 256, 636, 994
NlaIII CATG 7 cut(s) 291, 327, 437, 643, 790, 811, 1245
NlaIV GGNNCC 3 cut(s) 258, 750, 819
NmuCI GTSAC 5 cut(s) 154, 463, 532, 803, 1133
PagI TCATGA 1 cut(s) 639
PaqCI CACCTGC 1 cut(s) 998
PciSI GCTCTTC 1 cut(s) 1013
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 2 cut(s) 620, 782
PflMI CCANNNNNTGG 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 826
PkrI GCNGC 3 cut(s) 195, 430, 1183
PleI GAGTC 4 cut(s) 151, 529, 947, 1193
PpsI GAGTC 4 cut(s) 151, 529, 947, 1193
PsiI TTATAA 2 cut(s) 29, 735
Psp6I CCWGG 2 cut(s) 625, 826
PspGI CCWGG 2 cut(s) 625, 826
PspN4I GGNNCC 3 cut(s) 258, 750, 819
PstI CTGCAG 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 2 cut(s) 278, 890
RsaNI GTAC 2 cut(s) 277, 889
RseI CAYNNNNRTG 1 cut(s) 1090
SapI GCTCTTC 1 cut(s) 1013
SaqAI TTAA 6 cut(s) 336, 369, 540, 839, 921, 1103
SatI GCNGC 3 cut(s) 194, 429, 1182
Sau3AI GATC 4 cut(s) 235, 256, 636, 994
SchI GAGTC 4 cut(s) 151, 529, 947, 1193
ScrFI CCNGG 2 cut(s) 627, 828
SfaNI GCATC 2 cut(s) 108, 956
SfcI CTRYAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 1090
SmlI CTYRAG 1 cut(s) 983
SmoI CTYRAG 1 cut(s) 983
Sse9I AATT 7 cut(s) 485, 736, 790, 836, 881, 1185, 1227
SsiI CCGC 2 cut(s) 268, 1263
SspI AATATT 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 625, 826
StyI CCWWGG 1 cut(s) 1241
TaaI ACNGT 5 cut(s) 104, 377, 1128, 1160, 1178
TaiI ACGT 1 cut(s) 470
TaqI TCGA 1 cut(s) 1098
TasI AATT 7 cut(s) 485, 736, 790, 836, 881, 1185, 1227
TatI WGTACW 2 cut(s) 276, 888
TfiI GAWTC 2 cut(s) 620, 782
Tru1I TTAA 6 cut(s) 336, 369, 540, 839, 921, 1103
Tru9I TTAA 6 cut(s) 336, 369, 540, 839, 921, 1103
TscAI CASTG 2 cut(s) 380, 931
TseFI GTSAC 5 cut(s) 154, 463, 532, 803, 1133
TseI GCWGC 3 cut(s) 193, 428, 1181
Tsp45I GTSAC 5 cut(s) 154, 463, 532, 803, 1133
TspDTI ATGAA 6 cut(s) 27, 261, 276, 729, 795, 803
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 2 cut(s) 380, 931
Van91I CCANNNNNTGG 1 cut(s) 288
XapI RAATTY 2 cut(s) 485, 881
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 822
ZraI GACGTC 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.