RchiOBHm_Chr5g0065361

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009849
Physical Location & Seq
Forward (+)
71213252 .. 71217930
4679 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34124

Sequence Viewer

Length: 1152 bp
ATGAGTATCCCAAACAAGGATGAGAAGAACAGCAAGGGCTTCTTCGCCGCCATGAGTTCCGGCCTCTCCGTCTTCGGCAGCGCCATGCACCGATCCGTTGGCGGGATGCTTGGTTATGAAGGGGTGGAAGTTATAAATCCAGAGGGAGGCAAAGAAGACGCAGAGGAGGAAGCTCAACGAGGAAGATGGAAACAGGAGGAACGAGACAGTTACTGGAAGATGATGCAAAAGTATATAGGTTCAGATATAACATCAATGGTGACTCTTCCTCTGATGGAATATTCACACTTGTTGGATCGTGCTGATGAATGTGAGGATCCTTATATGCGGTTGGTGTACACCACTTCATGGGCTATATCTGTCTACTACGCCTACCAGCGAACATGGAAACCATTTAATCCTATACTTGGGGAGACTTATGAGATGGTTAATCACTGTGGGATTACATTCGTTGCAGAGCAGGTCAGTCATCATCCACCAATGAGTGCTGCTCATGGTGAAAATGAGCATTTCACTTATGATGTGACGTCAAAGCTGAAAACTAAATTTTTAGGGAACTCTCTTGATGTTTACCCTGTTGGAAGAACCCGAGTGACTCTTAAAAGAGATGGTGTAGTCTTAGATTTAGTGCCACCTCCCACAAAGGTAAACAACTTGATATTCGGACGGACTTGGGTTGATTCACCTGGAGAGATGATCATGACAAATCTGACAACTGGGGACAAAGCTGTGCTATGTTTCCAGCCTTGTGGCTGGTTTGGAGCTGGTCGTTATGAAGTGGATGGATATGTTTATAATTCTGCTGAGGAACCTAAGATATTGATGACTGGGAAATGGAATGAATCCATGAATTATCAACCTTGTGATATGGAAGGGGAACCCCTTCCAGGAACAGAATTAAAAGAGATTGGAGGAGAGCAGAGAGCTGAAAAGAGAGAACGAGAAGCAAAGGAACAAAAGTTTACACCAAGATGGTTCGACTTAACTGAGGAGGTTACACCTACACCGTGGGGTGACTTGGAAGTCTATCAGTATAACAGTAAATACACTCAACACCGTGCTGCAATTGATAGCTCTGACTCTGTTGATGGGGTTGATGTGAAATCAATTGACTTCAACCCATGGCAGTATGGCAATGTGTCCGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

383

Amino Acids

43.53

Weight (kDa)

4.94

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 72 - 287 1.2e-65 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 134, 795
AasI GACNNNNNNGTC 1 cut(s) 1022
AatII GACGTC 1 cut(s) 530
Acc36I ACCTGC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 348
AccI GTMKAC 1 cut(s) 363
AciI CCGC 4 cut(s) 48, 102, 328, 1143
AclWI GGATC 4 cut(s) 87, 303, 311, 324
AcsI RAATTY 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 348, 407, 887
AgsI TTSAA 1 cut(s) 1117
AjnI CCWGG 2 cut(s) 685, 886
AluBI AGCT 6 cut(s) 173, 535, 728, 764, 926, 1074
AluI AGCT 6 cut(s) 173, 535, 728, 764, 926, 1074
Alw26I GTCTC 2 cut(s) 198, 407
AlwI GGATC 4 cut(s) 87, 303, 311, 324
AlwNI CAGNNNCTG 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 78, 488, 1061
ApoI RAATTY 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 1 cut(s) 83
AsuHPI GGTGA 4 cut(s) 271, 509, 675, 1025
AvaI CYCGRG 1 cut(s) 588
BamHI GGATCC 1 cut(s) 316
BbsI GAAGAC 2 cut(s) 64, 162
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 3 cut(s) 90, 475, 1048
BccI CCATC 7 cut(s) 180, 268, 418, 602, 776, 966, 1082
BciT130I CCWGG 2 cut(s) 687, 888
BciVI GTATCC 1 cut(s) 17
BclI TGATCA 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 198, 407
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 17
BisI GCNGC 4 cut(s) 48, 79, 489, 1062
BlsI GCNGC 4 cut(s) 49, 80, 490, 1063
Bme1390I CCNGG 2 cut(s) 687, 888
BmeT110I CYCGRG 1 cut(s) 588
BmiI GGNNCC 3 cut(s) 318, 810, 879
BmrFI CCNGG 2 cut(s) 687, 888
BmrI ACTGGG 2 cut(s) 726, 837
BmsI GCATC 2 cut(s) 96, 213
BmuI ACTGGG 2 cut(s) 726, 837
BpiI GAAGAC 2 cut(s) 64, 162
BplI GAGNNNNNCTC 2 cut(s) 475, 507
BpmI CTGGAG 1 cut(s) 708
Bpu10I CCTNAGC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 2 cut(s) 1007, 1121
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 102, 146, 348, 407, 887
Bse1I ACTGG 3 cut(s) 218, 721, 832
Bse3DI GCAATG 1 cut(s) 1141
BseBI CCWGG 2 cut(s) 687, 888
BseDI CCNNGG 2 cut(s) 1007, 1121
BseGI GGATG 4 cut(s) 25, 111, 472, 787
BseLI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 348, 407, 887
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 2 cut(s) 795, 978
BseNI ACTGG 3 cut(s) 218, 721, 832
BseRI GAGGAG 3 cut(s) 179, 927, 1004
BseXI GCAGC 3 cut(s) 90, 475, 1048
BshFI GGCC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 1 cut(s) 60
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 6 cut(s) 16, 102, 146, 348, 407, 887
BsmAI GTCTC 2 cut(s) 198, 407
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 588
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 4 cut(s) 92, 295, 316, 696
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 4 cut(s) 48, 102, 328, 1143
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 2 cut(s) 796, 979
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 3 cut(s) 318, 810, 879
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 4 cut(s) 87, 303, 311, 324
BsrDI GCAATG 1 cut(s) 1141
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 3 cut(s) 218, 721, 832
BssECI CCNNGG 2 cut(s) 1007, 1121
BssMI GATC 4 cut(s) 92, 295, 316, 696
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 2 cut(s) 687, 888
Bst4CI ACNGT 5 cut(s) 209, 437, 1008, 1040, 1058
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 527
BstAUI TGTACA 1 cut(s) 336
BstDEI CTNAG 4 cut(s) 619, 804, 813, 987
BstDSI CCRYGG 2 cut(s) 1007, 1121
BstF5I GGATG 4 cut(s) 25, 111, 472, 787
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 4 cut(s) 95, 298, 319, 699
BstMAI GTCTC 2 cut(s) 198, 407
BstMBI GATC 4 cut(s) 92, 295, 316, 696
BstNI CCWGG 2 cut(s) 687, 888
BstSCI CCNGG 2 cut(s) 685, 886
BstV1I GCAGC 3 cut(s) 90, 475, 1048
BstV2I GAAGAC 2 cut(s) 64, 162
BstX2I RGATCY 1 cut(s) 316
BstXI CCANNNNNNTGG 1 cut(s) 749
BstYI RGATCY 1 cut(s) 316
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 2 cut(s) 1007, 1121
BtsCI GGATG 4 cut(s) 25, 111, 472, 787
BtsIMutI CAGTG 1 cut(s) 433
BveI ACCTGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 213
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 83
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 337
CviAII CATG 8 cut(s) 52, 85, 348, 384, 494, 700, 847, 1122
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 4 cut(s) 619, 804, 813, 987
DpnI GATC 4 cut(s) 94, 297, 318, 698
DpnII GATC 4 cut(s) 92, 295, 316, 696
DrdI GACNNNNNNGTC 1 cut(s) 1022
DseDI GACNNNNNNGTC 1 cut(s) 1022
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 1121
Eco88I CYCGRG 1 cut(s) 588
EcoRII CCWGG 2 cut(s) 685, 886
EcoT14I CCWWGG 1 cut(s) 1121
ErhI CCWWGG 1 cut(s) 1121
FaeI CATG 8 cut(s) 55, 88, 351, 387, 497, 703, 850, 1125
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 734
FatI CATG 8 cut(s) 51, 84, 347, 383, 493, 699, 846, 1121
FauI CCCGC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 696
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 48, 79, 489, 1062
FokI GGATG 4 cut(s) 32, 118, 459, 794
Fsp4HI GCNGC 4 cut(s) 48, 79, 489, 1062
GlaI GCGC 1 cut(s) 82
GluI GCNGC 4 cut(s) 48, 79, 489, 1062
GsuI CTGGAG 1 cut(s) 708
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 60
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 83
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 8 cut(s) 55, 88, 351, 387, 497, 703, 850, 1125
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 5 cut(s) 262, 595, 680, 842, 1079
HpaII CCGG 1 cut(s) 60
HphI GGTGA 4 cut(s) 271, 509, 675, 1025
Hpy166II GTNNAC 6 cut(s) 337, 339, 364, 571, 649, 963
Hpy188I TCNGA 5 cut(s) 244, 273, 665, 711, 1078
Hpy188III TCNNGA 3 cut(s) 140, 563, 700
Hpy8I GTNNAC 6 cut(s) 337, 339, 364, 571, 649, 963
HpyAV CCTTC 3 cut(s) 113, 866, 893
HpyCH4III ACNGT 5 cut(s) 209, 437, 1008, 1040, 1058
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 4 cut(s) 88, 226, 455, 1064
HpyF3I CTNAG 4 cut(s) 619, 804, 813, 987
HpySE526I ACGT 1 cut(s) 527
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 8 cut(s) 55, 88, 351, 387, 497, 703, 850, 1125
HspAI GCGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 4 cut(s) 92, 295, 316, 696
LmnI GCTCC 1 cut(s) 761
Lsp1109I GCAGC 3 cut(s) 90, 475, 1048
LweI GCATC 2 cut(s) 96, 213
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 6 cut(s) 209, 259, 523, 592, 994, 1013
MalI GATC 4 cut(s) 94, 297, 318, 698
MboI GATC 4 cut(s) 92, 295, 316, 696
MboII GAAGA 8 cut(s) 34, 37, 64, 167, 195, 229, 257, 594
MfeI CAATTG 2 cut(s) 1065, 1107
MflI RGATCY 1 cut(s) 316
MluCI AATT 6 cut(s) 545, 796, 850, 896, 1065, 1107
MlyI GAGTC 3 cut(s) 256, 589, 1073
MmeI TCCRAC 2 cut(s) 273, 559
MroXI GAANNNNTTC 1 cut(s) 882
MseI TTAA 5 cut(s) 396, 429, 600, 899, 983
MslI CAYNNNNRTG 1 cut(s) 970
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 2 cut(s) 687, 888
MunI CAATTG 2 cut(s) 1065, 1107
MvaI CCWGG 2 cut(s) 687, 888
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 4 cut(s) 92, 295, 316, 696
NlaIII CATG 8 cut(s) 55, 88, 351, 387, 497, 703, 850, 1125
NlaIV GGNNCC 3 cut(s) 318, 810, 879
NmuCI GTSAC 4 cut(s) 259, 523, 592, 1013
PagI TCATGA 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 2 cut(s) 680, 842
PflMI CCANNNNNTGG 1 cut(s) 348
PfoI TCCNGGA 1 cut(s) 886
PkrI GCNGC 4 cut(s) 49, 80, 490, 1063
PleI GAGTC 3 cut(s) 256, 589, 1073
PpsI GAGTC 3 cut(s) 256, 589, 1073
PsiI TTATAA 2 cut(s) 134, 795
Psp6I CCWGG 2 cut(s) 685, 886
PspGI CCWGG 2 cut(s) 685, 886
PspN4I GGNNCC 3 cut(s) 318, 810, 879
PstNI CAGNNNCTG 1 cut(s) 213
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 970
SaqAI TTAA 5 cut(s) 396, 429, 600, 899, 983
SatI GCNGC 4 cut(s) 48, 79, 489, 1062
Sau3AI GATC 4 cut(s) 92, 295, 316, 696
SchI GAGTC 3 cut(s) 256, 589, 1073
ScrFI CCNGG 2 cut(s) 687, 888
SfaNI GCATC 2 cut(s) 96, 213
SmiMI CAYNNNNRTG 1 cut(s) 970
Sse9I AATT 6 cut(s) 545, 796, 850, 896, 1065, 1107
SsiI CCGC 4 cut(s) 48, 102, 328, 1143
SspI AATATT 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 685, 886
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 5 cut(s) 209, 437, 1008, 1040, 1058
TaiI ACGT 1 cut(s) 530
TaqI TCGA 1 cut(s) 978
TasI AATT 6 cut(s) 545, 796, 850, 896, 1065, 1107
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 680, 842
Tru1I TTAA 5 cut(s) 396, 429, 600, 899, 983
Tru9I TTAA 5 cut(s) 396, 429, 600, 899, 983
TscAI CASTG 1 cut(s) 440
TseFI GTSAC 4 cut(s) 259, 523, 592, 1013
TseI GCWGC 3 cut(s) 78, 488, 1061
Tsp45I GTSAC 4 cut(s) 259, 523, 592, 1013
TspDTI ATGAA 6 cut(s) 132, 321, 336, 789, 855, 863
TspGWI ACGGA 3 cut(s) 58, 85, 682
TspRI CASTG 1 cut(s) 440
Van91I CCANNNNNTGG 1 cut(s) 348
XapI RAATTY 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 882
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.