Prupe.6G074200_v2.0.a1

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009849
Physical Location & Seq
Reverse (-)
5072746 .. 5078657
5912 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G074200.2

Sequence Viewer

Length: 1371 bp
ATGGTTAGCCCAAAGAAGGAACAGAGCAACGGCTTCTTCGCAGCCATGAATTCTGGCCTCTCCGTGTTCAGCAACGCCATGCACCGATCTGTTGGCGGGTTGCTTGGTTATGAAGGGGTGGAAGTCATAAATCCAGAGGGAGGCAAAGAAGATGCAGAGGAGGAAGCTCATAGAGGAAGATGGAAGCAGGAGGAACGAGATAGTTACTGGAGGATGATGCAGAAGTATATAGGTTCAGATGTAACATCAATGGTGACTCTTCCTGTACTTATTTTTGAGCCCATGACAATGCTGCAGAAAATGGCCGAGTTAATGGAGTATTCGAACTTGTTGGATCGCGCAGACGAATGTGAGGATCCTTATTTGCGGTTGGTGTTTGCCACGTCATGGGCTATATCCGTCTACTATGCCTACCAGCGAACATGGAAGCCATTCAATCCTATCCTTGGGGAAACTTATGAAATGGTTAATCACTCAGGGGTTACATTTATTGCAGAGCAGGTGAGTCATCATCCACCAATGAGTGCTGGGCATGCTGAAAATGAGCATTTTGCTTATGACGTGACATCAAAGCTGAAAACTAAATTTTTAGGCAACTCTGTTGATGTTTACCCTGTTGGAAGAACACGCGTTACTCTTAAAAGAGATGGTGTAGTCTTAGACTTAGTGCCACCTCCCACAAAGGTAAACAACTTAATATTCGGCCGGACGTGGGTTGATTCACCAGGGGAGATGATCATGACCAATCTGACGACTGGGGACAAAGCTGTGCTATATTTTCAGCCATGTGGCTGGTTTGGAGCTGGCCGCTATGAAGTGGATGGATATGTTTACAATGCTGCTGAGGAACCTCAGATATTGATGACTGGGAAATGGAATGAGTCAATGAGTTATCAACCATGTGATATGGAAGGGGAACCCCTTCCAGGAACAGAATTAAAAGAGGTTTGGCATATTGCTGATGCTCCAAAAAATGACAAATTTCAGTACACATATTTTGCACATAAAATAAATAGCTTTGACACCGCTCCTAAAAGGTTGTTGGCATCAGACTCTCGTTTGCGGCCTGATAGATATGCACTTGAGAAAGGTGATCTATCCAAATCAGGTGCAGAAAAGAGCAGATTGGAGGAGAGGCAGAGAGCCGAAAAGAGAGAACGAGAGGCCAAGCATCATCAGTTTACACCACGATGGTTTGACTTGACTGAGGAAGTTACACCTACACCTTGGGGTGACTTGGAAGTCTATCAATATAACAATAAATACAATGAACACCGAGCAGCAGTCGATAGCTCTGACTCTATTGATGAGGTTGATGTTAAACTAATTGAATTCAACCCATGGCAGTATGGTAATTTGTCTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

457

Amino Acids

52.25

Weight (kDa)

5.19

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 490
AasI GACNNNNNNGTC 1 cut(s) 1241
Acc36I ACCTGC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 402
AccII CGCG 2 cut(s) 339, 630
AciI CCGC 5 cut(s) 96, 367, 808, 1026, 1063
AclWI GGATC 3 cut(s) 342, 350, 363
AcoI YGGCCR 3 cut(s) 303, 703, 805
AcsI RAATTY 4 cut(s) 49, 584, 980, 1331
AfaI GTAC 2 cut(s) 267, 989
AfiI CCNNNNNNNGG 6 cut(s) 16, 140, 387, 446, 712, 926
AflIII ACRYGT 1 cut(s) 628
AgsI TTSAA 3 cut(s) 436, 1331, 1336
AjiI CACGTC 3 cut(s) 384, 562, 711
AjnI CCWGG 2 cut(s) 724, 925
AluBI AGCT 6 cut(s) 167, 574, 767, 803, 1017, 1293
AluI AGCT 6 cut(s) 167, 574, 767, 803, 1017, 1293
AlwI GGATC 3 cut(s) 342, 350, 363
AoxI GGCC 6 cut(s) 55, 303, 703, 805, 1064, 1164
ApeKI GCWGC 4 cut(s) 41, 292, 839, 1280
ApoI RAATTY 4 cut(s) 49, 584, 980, 1331
Asp700I GAANNNNTTC 2 cut(s) 431, 921
AspLEI GCGC 1 cut(s) 341
AsuHPI GGTGA 5 cut(s) 265, 514, 714, 1103, 1244
AsuII TTCGAA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 4 cut(s) 53, 279, 826, 1292
BccI CCATC 4 cut(s) 174, 641, 815, 1185
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 2 cut(s) 726, 927
BclI TGATCA 1 cut(s) 735
BfmI CTRYAG 2 cut(s) 293, 1362
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 6 cut(s) 42, 293, 808, 840, 1064, 1281
BlsI GCNGC 6 cut(s) 43, 294, 809, 841, 1065, 1282
Bme1390I CCNGG 2 cut(s) 726, 927
BmgBI CACGTC 3 cut(s) 384, 562, 711
BmiI GGNNCC 3 cut(s) 357, 849, 918
BmrFI CCNGG 2 cut(s) 726, 927
BmrI ACTGGG 2 cut(s) 765, 876
BmsI GCATC 5 cut(s) 142, 207, 952, 1055, 1180
BmuI ACTGGG 2 cut(s) 765, 876
BpmI CTGGAG 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 1103
BsaJI CCNNGG 4 cut(s) 445, 725, 1226, 1340
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 140, 387, 446, 712, 926
Bse1I ACTGG 3 cut(s) 212, 760, 871
BseBI CCWGG 2 cut(s) 726, 927
BseDI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BseGI GGATG 3 cut(s) 219, 511, 826
BseLI CCNNNNNNNGG 6 cut(s) 16, 140, 387, 446, 712, 926
BseMII CTCAG 4 cut(s) 489, 834, 866, 1197
BseNI ACTGG 3 cut(s) 212, 760, 871
BseRI GAGGAG 2 cut(s) 173, 1145
BseX3I CGGCCG 1 cut(s) 703
BseXI GCAGC 4 cut(s) 53, 279, 826, 1292
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 1131
Bsh1236I CGCG 2 cut(s) 339, 630
Bsh1285I CGRYCG 1 cut(s) 706
BshFI GGCC 6 cut(s) 57, 305, 705, 807, 1066, 1166
BsiEI CGRYCG 1 cut(s) 706
BsiSI CCGG 1 cut(s) 706
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 6 cut(s) 16, 140, 387, 446, 712, 926
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 6 cut(s) 57, 305, 705, 807, 1066, 1166
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 5 cut(s) 86, 334, 355, 735, 1093
Bsp19I CCATGG 1 cut(s) 1340
BspACI CCGC 5 cut(s) 96, 367, 808, 1026, 1063
BspANI GGCC 6 cut(s) 57, 305, 705, 807, 1066, 1166
BspCNI CTCAG 4 cut(s) 488, 835, 865, 1198
BspFNI CGCG 2 cut(s) 339, 630
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 3 cut(s) 357, 849, 918
BspMAI CTGCAG 2 cut(s) 297, 1366
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 3 cut(s) 342, 350, 363
BspT104I TTCGAA 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 1028
BsrI ACTGG 3 cut(s) 212, 760, 871
BssECI CCNNGG 4 cut(s) 445, 725, 1226, 1340
BssMI GATC 5 cut(s) 86, 334, 355, 735, 1093
BssT1I CCWWGG 3 cut(s) 445, 1226, 1340
Bst2UI CCWGG 2 cut(s) 726, 927
Bst6I CTCTTC 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 534, 805
BstDEI CTNAG 6 cut(s) 475, 658, 664, 843, 852, 1206
BstDSI CCRYGG 1 cut(s) 1340
BstF5I GGATG 3 cut(s) 219, 511, 826
BstFNI CGCG 2 cut(s) 339, 630
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 5 cut(s) 89, 337, 358, 738, 1096
BstMBI GATC 5 cut(s) 86, 334, 355, 735, 1093
BstMCI CGRYCG 1 cut(s) 706
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 2 cut(s) 726, 927
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 724, 925
BstSFI CTRYAG 2 cut(s) 293, 1362
BstUI CGCG 2 cut(s) 339, 630
BstV1I GCAGC 4 cut(s) 53, 279, 826, 1292
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 1 cut(s) 355
BstZI CGGCCG 1 cut(s) 703
BsuRI GGCC 6 cut(s) 57, 305, 705, 807, 1066, 1166
BtgI CCRYGG 1 cut(s) 1340
BtrI CACGTC 3 cut(s) 384, 562, 711
BtsCI GGATG 3 cut(s) 219, 511, 826
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 534, 805
CciI TCATGA 1 cut(s) 738
CfoI GCGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 266, 988
CviQI GTAC 2 cut(s) 266, 988
DdeI CTNAG 6 cut(s) 475, 658, 664, 843, 852, 1206
DpnI GATC 5 cut(s) 88, 336, 357, 737, 1095
DpnII GATC 5 cut(s) 86, 334, 355, 735, 1093
DrdI GACNNNNNNGTC 1 cut(s) 1241
DseDI GACNNNNNNGTC 1 cut(s) 1241
EaeI YGGCCR 3 cut(s) 303, 703, 805
EagI CGGCCG 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EclXI CGGCCG 1 cut(s) 703
Eco130I CCWWGG 3 cut(s) 445, 1226, 1340
Eco24I GRGCYC 1 cut(s) 282
Eco52I CGGCCG 1 cut(s) 703
EcoRI GAATTC 2 cut(s) 49, 1331
EcoRII CCWGG 2 cut(s) 724, 925
EcoT14I CCWWGG 3 cut(s) 445, 1226, 1340
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 3 cut(s) 445, 1226, 1340
FaqI GGGAC 1 cut(s) 773
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 6 cut(s) 42, 293, 808, 840, 1064, 1281
FokI GGATG 3 cut(s) 226, 498, 833
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 6 cut(s) 42, 293, 808, 840, 1064, 1281
GlaI GCGC 1 cut(s) 340
GluI GCNGC 6 cut(s) 42, 293, 808, 840, 1064, 1281
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 6 cut(s) 57, 305, 705, 807, 1066, 1166
HapII CCGG 1 cut(s) 706
HhaI GCGC 1 cut(s) 341
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HinfI GANTC 6 cut(s) 256, 505, 719, 881, 1052, 1298
HpaII CCGG 1 cut(s) 706
HphI GGTGA 5 cut(s) 265, 514, 714, 1103, 1244
Hpy166II GTNNAC 6 cut(s) 403, 610, 688, 832, 990, 1182
Hpy188I TCNGA 5 cut(s) 238, 750, 855, 1051, 1297
Hpy188III TCNNGA 2 cut(s) 134, 739
Hpy8I GTNNAC 6 cut(s) 403, 610, 688, 832, 990, 1182
HpyAV CCTTC 4 cut(s) 10, 107, 905, 932
HpyCH4IV ACGT 3 cut(s) 383, 561, 710
HpyCH4V TGCA 9 cut(s) 82, 155, 220, 295, 494, 1001, 1079, 1112, 1364
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 6 cut(s) 475, 658, 664, 843, 852, 1206
HpySE526I ACGT 3 cut(s) 383, 561, 710
HspAI GCGC 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 5 cut(s) 86, 334, 355, 735, 1093
LmnI GCTCC 3 cut(s) 800, 970, 1033
Lsp1109I GCAGC 4 cut(s) 53, 279, 826, 1292
LweI GCATC 5 cut(s) 142, 207, 952, 1055, 1180
MaeII ACGT 3 cut(s) 383, 561, 710
MaeIII GTNAC 8 cut(s) 203, 241, 253, 481, 562, 631, 1213, 1232
MalI GATC 5 cut(s) 88, 336, 357, 737, 1095
MbiI CCGCTC 1 cut(s) 1028
MboI GATC 5 cut(s) 86, 334, 355, 735, 1093
MboII GAAGA 5 cut(s) 28, 161, 189, 251, 633
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 7 cut(s) 49, 584, 935, 980, 1326, 1331, 1354
MluI ACGCGT 1 cut(s) 628
MlyI GAGTC 5 cut(s) 250, 514, 890, 1046, 1292
MmeI TCCRAC 2 cut(s) 312, 598
MroXI GAANNNNTTC 2 cut(s) 431, 921
MseI TTAA 6 cut(s) 311, 468, 639, 695, 938, 1320
MslI CAYNNNNRTG 2 cut(s) 287, 1189
MspI CCGG 1 cut(s) 706
MspR9I CCNGG 2 cut(s) 726, 927
MvaI CCWGG 2 cut(s) 726, 927
MvnI CGCG 2 cut(s) 339, 630
MwoI GCNNNNNNNGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 1340
NdeII GATC 5 cut(s) 86, 334, 355, 735, 1093
NlaIV GGNNCC 3 cut(s) 357, 849, 918
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 3 cut(s) 253, 562, 1232
NspI RCATGY 1 cut(s) 536
NspV TTCGAA 1 cut(s) 323
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 1 cut(s) 738
PaqCI CACCTGC 1 cut(s) 490
PdmI GAANNNNTTC 2 cut(s) 431, 921
PfeI GAWTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 925
PkrI GCNGC 6 cut(s) 43, 294, 809, 841, 1065, 1282
PleI GAGTC 5 cut(s) 250, 513, 889, 1046, 1292
PpsI GAGTC 5 cut(s) 250, 513, 889, 1046, 1292
Psp6I CCWGG 2 cut(s) 724, 925
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 724, 925
PspN4I GGNNCC 3 cut(s) 357, 849, 918
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PstI CTGCAG 2 cut(s) 297, 1366
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 267, 989
RsaNI GTAC 2 cut(s) 266, 988
RseI CAYNNNNRTG 2 cut(s) 287, 1189
SaqAI TTAA 6 cut(s) 311, 468, 639, 695, 938, 1320
SatI GCNGC 6 cut(s) 42, 293, 808, 840, 1064, 1281
Sau3AI GATC 5 cut(s) 86, 334, 355, 735, 1093
SchI GAGTC 5 cut(s) 250, 514, 890, 1046, 1292
ScrFI CCNGG 2 cut(s) 726, 927
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 5 cut(s) 142, 207, 952, 1055, 1180
SfcI CTRYAG 2 cut(s) 293, 1362
SfuI TTCGAA 1 cut(s) 323
SmiMI CAYNNNNRTG 2 cut(s) 287, 1189
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SphI GCATGC 1 cut(s) 536
Sse9I AATT 7 cut(s) 49, 584, 935, 980, 1326, 1331, 1354
SsiI CCGC 5 cut(s) 96, 367, 808, 1026, 1063
SspI AATATT 1 cut(s) 699
StyD4I CCNGG 2 cut(s) 724, 925
StyI CCWWGG 3 cut(s) 445, 1226, 1340
TaiI ACGT 3 cut(s) 386, 564, 713
TaqI TCGA 2 cut(s) 323, 1287
TasI AATT 7 cut(s) 49, 584, 935, 980, 1326, 1331, 1354
TatI WGTACW 2 cut(s) 265, 987
TauI GCSGC 2 cut(s) 810, 1066
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 6 cut(s) 311, 468, 639, 695, 938, 1320
Tru9I TTAA 6 cut(s) 311, 468, 639, 695, 938, 1320
TseFI GTSAC 3 cut(s) 253, 562, 1232
TseI GCWGC 4 cut(s) 41, 292, 839, 1280
Tsp45I GTSAC 3 cut(s) 253, 562, 1232
TspDTI ATGAA 5 cut(s) 62, 126, 474, 828, 1284
TspGWI ACGGA 2 cut(s) 52, 388
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 4 cut(s) 49, 584, 980, 1331
XceI RCATGY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 431, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.