AT5G60850

Dof domain, zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015444
Physical Location & Seq
Forward (+)
24480291 .. 24482163
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60850.1

Sequence Viewer

Length: 924 bp
ATGCAAGATATTCATGATTTCTCCATGAACGGAGTTGGTGGTGGGGGAGGAGGAGGAGGGAGGTTTTTCGGTGGAGGAATCGGCGGCGGAGGAGGTGGTGATCGAAGGATGAGAGCTCATCAGAACAATATACTTAACCATCATCAATCTCTCAAGTGTCCTCGTTGTAATTCTCTTAACACAAAGTTCTGTTACTACAACAATTACAATCTTTCTCAGCCTCGTCACTTTTGCAAGAACTGTCGTCGTTACTGGACTAAAGGTGGTGTTCTCCGTAACGTTCCCGTCGGAGGTGGTTGCCGGAAAGCTAAACGTTCGAAAACAAAACAGGTTCCGTCGTCGTCATCAGCCGACAAACCAACGACGACGCAAGATGATCATCACGTGGAGGAGAAATCGAGTACAGGATCTCACTCTAGCAGCGAGAGCTCTTCTCTCACCGCTTCTAACTCTACCACCGTCGCCGCCGTCTCCGTCACCGCGGCGGCGGAAGTTGCTTCGTCGGTTATTCCAGGTTTTGATATGCCTAATATGAAAATTTACGGTAACGGGATCGAGTGGTCGACGTTACTTGGACAAGGCTCATCGGCCGGTGGTGTTTTCTCGGAGATCGGTGGTTTTCCGGCGGTTTCAGCTATTGAAACTACACCGTTTGGATTCGGGGGTAAATTCGTAAATCAAGATGATCATCTGAAGTTAGAAGGTGAAACTGTACAGCAGCAACAGTTTGGAGATCGAACGGCTCAGGTTGAGTTTCAAGGAAGATCTTCGGATCCGAATATGGGATTTGAACCGTTGGATTGGGGAAGTGGCGGTGGAGATCAAACACTGTTTGATTTAACCAGTACCGTTGATCATGCATACTGGAGTCAAAGTCAATGGACGTCGTCTGACCAAGATCAGAGTGGTCTCTACCTTCCTTGA

Protein Analysis

307

Amino Acids

32.74

Weight (kDa)

6.54

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 49 - 105 2.1e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60850
fragaria_vesca FvH4_5g13390
malus_domestica MD06G1205900.v1.1 MD14G1216800.v1.1
prunus_persica Prupe.5G210200_v2.0.a1
pyrus_communis pycom06g18370 pycom14g17960
rosa_chinensis RchiOBHm_Chr7g0183751
rosa_laevigata RLG00000005026
rosa_multiflora Rmu_sc0003610.1_g000031
rosa_rugosa Rorug06G0460000
rosa_samantha Rh7AG060700 Rh7BG060800 Rh7CG062400 Rh7DG060800
rosa_wichuraiana Rw7G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 887
AccI GTMKAC 1 cut(s) 563
AccII CGCG 1 cut(s) 482
AclI AACGTT 2 cut(s) 279, 313
AclWI GGATC 4 cut(s) 415, 560, 767, 780
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 2 cut(s) 537, 668
AcuI CTGAAG 1 cut(s) 713
AcvI CACGTG 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 884
AfaI GTAC 3 cut(s) 403, 714, 847
AfiI CCNNNNNNNGG 2 cut(s) 290, 782
AgsI TTSAA 3 cut(s) 641, 758, 791
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 4 cut(s) 116, 308, 429, 635
AluI AGCT 4 cut(s) 116, 308, 429, 635
Alw21I GWGCWC 2 cut(s) 118, 431
Alw26I GTCTC 2 cut(s) 475, 914
AlwI GGATC 4 cut(s) 415, 560, 767, 780
AoxI GGCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 420, 718
ApoI RAATTY 2 cut(s) 537, 668
Asp700I GAANNNNTTC 1 cut(s) 766
AsuHPI GGTGA 4 cut(s) 110, 430, 469, 716
AsuII TTCGAA 1 cut(s) 317
BamHI GGATCC 1 cut(s) 772
BanII GRGCYC 2 cut(s) 118, 431
BbrPI CACGTG 1 cut(s) 385
Bbv12I GWGCWC 2 cut(s) 118, 431
BbvI GCAGC 2 cut(s) 432, 730
BccI CCATC 1 cut(s) 147
BceAI ACGGC 2 cut(s) 452, 756
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 3 cut(s) 376, 685, 853
BcoDI GTCTC 2 cut(s) 475, 914
BfaI CTAG 1 cut(s) 417
BglII AGATCT 1 cut(s) 764
BisI GCNGC 6 cut(s) 85, 421, 465, 483, 486, 719
BlsI GCNGC 6 cut(s) 86, 422, 466, 484, 487, 720
Bme1390I CCNGG 1 cut(s) 513
BmiI GGNNCC 2 cut(s) 333, 774
BmrFI CCNGG 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 418, 450
BpmI CTGGAG 1 cut(s) 886
Bpu10I CCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 2 cut(s) 378, 687
BsaHI GRCGYC 1 cut(s) 884
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 1 cut(s) 480
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 782
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 3 cut(s) 257, 843, 869
Bse8I GATNNNNATC 2 cut(s) 378, 687
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 1 cut(s) 114
BseJI GATNNNNATC 2 cut(s) 378, 687
BseLI CCNNNNNNNGG 2 cut(s) 290, 782
BseMII CTCAG 2 cut(s) 230, 758
BseNI ACTGG 3 cut(s) 257, 843, 869
BseRI GAGGAG 5 cut(s) 63, 66, 69, 105, 404
BseX3I CGGCCG 1 cut(s) 588
BseXI GCAGC 2 cut(s) 432, 730
Bsh1236I CGCG 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 591
BshFI GGCC 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 591
BsiHKAI GWGCWC 2 cut(s) 118, 431
BsiSI CCGG 3 cut(s) 301, 591, 623
BslI CCNNNNNNNGG 2 cut(s) 290, 782
BsmAI GTCTC 2 cut(s) 475, 914
BsmBI CGTCTC 1 cut(s) 475
BsnI GGCC 1 cut(s) 590
Bso31I GGTCTC 1 cut(s) 914
Bsp119I TTCGAA 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 118, 431
Bsp1407I TGTACA 1 cut(s) 712
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 2 cut(s) 229, 757
BspFNI CGCG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 333, 774
BspPI GGATC 4 cut(s) 415, 560, 767, 780
BspQI GCTCTTC 1 cut(s) 436
BspT104I TTCGAA 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 914
BsrFI RCCGGY 1 cut(s) 590
BsrGI TGTACA 1 cut(s) 712
BsrI ACTGG 3 cut(s) 257, 843, 869
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 1 cut(s) 480
BssNI GRCGYC 1 cut(s) 884
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 9 cut(s) 242, 460, 545, 651, 712, 726, 795, 831, 850
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 712
BstBAI YACGTR 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 216, 744
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 482
BstMAI GTCTC 2 cut(s) 475, 914
BstMCI CGRYCG 1 cut(s) 591
BstMWI GCNNNNNNNGC 3 cut(s) 426, 494, 632
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 2 cut(s) 432, 730
BstX2I RGATCY 3 cut(s) 407, 764, 772
BstYI RGATCY 3 cut(s) 407, 764, 772
BstZI CGGCCG 1 cut(s) 588
BsuRI GGCC 1 cut(s) 590
BtgI CCRYGG 1 cut(s) 480
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 590
Cfr42I CCGCGG 1 cut(s) 483
CseI GACGC 1 cut(s) 376
Csp6I GTAC 3 cut(s) 402, 713, 846
CviAII CATG 3 cut(s) 14, 25, 857
CviJI RGCY 9 cut(s) 116, 220, 308, 350, 429, 582, 590, 635, 743
CviKI_1 RGCY 9 cut(s) 116, 220, 308, 350, 429, 582, 590, 635, 743
CviQI GTAC 3 cut(s) 402, 713, 846
DdeI CTNAG 2 cut(s) 216, 744
EaeI YGGCCR 1 cut(s) 588
EagI CGGCCG 1 cut(s) 588
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
EciI GGCGGA 2 cut(s) 102, 503
Ecl136II GAGCTC 2 cut(s) 116, 429
EclXI CGGCCG 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 118, 431
Eco31I GGTCTC 1 cut(s) 914
Eco52I CGGCCG 1 cut(s) 588
Eco53kI GAGCTC 2 cut(s) 116, 429
Eco57I CTGAAG 1 cut(s) 713
Eco72I CACGTG 1 cut(s) 385
EcoICRI GAGCTC 2 cut(s) 116, 429
EcoRII CCWGG 1 cut(s) 511
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 2 cut(s) 118, 431
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 3 cut(s) 17, 28, 860
FaiI YATR 8 cut(s) 15, 26, 131, 524, 533, 782, 858, 862
FatI CATG 3 cut(s) 13, 24, 856
FbaI TGATCA 3 cut(s) 376, 685, 853
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 6 cut(s) 85, 421, 465, 483, 486, 719
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 2 cut(s) 118, 431
Fsp4HI GCNGC 6 cut(s) 85, 421, 465, 483, 486, 719
FspBI CTAG 1 cut(s) 417
GluI GCNGC 6 cut(s) 85, 421, 465, 483, 486, 719
GsuI CTGGAG 1 cut(s) 886
HaeIII GGCC 1 cut(s) 590
HapII CCGG 3 cut(s) 301, 591, 623
HgaI GACGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 884
Hin1II CATG 3 cut(s) 17, 28, 860
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HinfI GANTC 3 cut(s) 78, 657, 868
HpaII CCGG 3 cut(s) 301, 591, 623
HphI GGTGA 4 cut(s) 110, 430, 469, 716
Hpy166II GTNNAC 1 cut(s) 564
Hpy188I TCNGA 8 cut(s) 123, 290, 607, 693, 772, 777, 892, 903
Hpy188III TCNNGA 2 cut(s) 14, 680
Hpy8I GTNNAC 1 cut(s) 564
HpyAV CCTTC 2 cut(s) 99, 695
HpyCH4III ACNGT 9 cut(s) 242, 460, 545, 651, 712, 726, 795, 831, 850
HpyCH4IV ACGT 5 cut(s) 279, 313, 384, 566, 884
HpyCH4V TGCA 3 cut(s) 4, 234, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 426, 494, 632
HpyF3I CTNAG 2 cut(s) 216, 744
HpySE526I ACGT 5 cut(s) 279, 313, 384, 566, 884
Hsp92I GRCGYC 1 cut(s) 884
Hsp92II CATG 3 cut(s) 17, 28, 860
Ksp22I TGATCA 3 cut(s) 376, 685, 853
KspI CCGCGG 1 cut(s) 483
LguI GCTCTTC 1 cut(s) 436
Lsp1109I GCAGC 2 cut(s) 432, 730
MaeI CTAG 1 cut(s) 417
MaeII ACGT 5 cut(s) 279, 313, 384, 566, 884
MaeIII GTNAC 7 cut(s) 191, 224, 248, 275, 475, 545, 567
MboII GAAGA 3 cut(s) 423, 759, 774
MflI RGATCY 3 cut(s) 407, 764, 772
MhlI GDGCHC 2 cut(s) 118, 431
MluCI AATT 4 cut(s) 169, 202, 537, 668
MlyI GAGTC 1 cut(s) 877
MmeI TCCRAC 2 cut(s) 268, 777
Mph1103I ATGCAT 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 766
MseI TTAA 3 cut(s) 135, 177, 839
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 3 cut(s) 301, 591, 623
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 3 cut(s) 426, 494, 632
NlaIII CATG 3 cut(s) 17, 28, 860
NlaIV GGNNCC 2 cut(s) 333, 774
NmuCI GTSAC 2 cut(s) 224, 475
NsiI ATGCAT 1 cut(s) 862
NspV TTCGAA 1 cut(s) 317
PagI TCATGA 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 766
PfeI GAWTC 2 cut(s) 78, 657
PflFI GACNNNGTC 1 cut(s) 886
PkrI GCNGC 6 cut(s) 86, 422, 466, 484, 487, 720
PleI GAGTC 1 cut(s) 876
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 1 cut(s) 876
Ppu21I YACGTR 1 cut(s) 385
Psp124BI GAGCTC 2 cut(s) 118, 431
Psp1406I AACGTT 2 cut(s) 279, 313
Psp6I CCWGG 1 cut(s) 511
PspCI CACGTG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 333, 774
PsuI RGATCY 3 cut(s) 407, 764, 772
PsyI GACNNNGTC 1 cut(s) 886
RsaI GTAC 3 cut(s) 403, 714, 847
RsaNI GTAC 3 cut(s) 402, 713, 846
SacI GAGCTC 2 cut(s) 118, 431
SacII CCGCGG 1 cut(s) 483
SalI GTCGAC 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 436
SaqAI TTAA 3 cut(s) 135, 177, 839
SatI GCNGC 6 cut(s) 85, 421, 465, 483, 486, 719
SchI GAGTC 1 cut(s) 877
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 2 cut(s) 118, 431
Sfr303I CCGCGG 1 cut(s) 483
SfuI TTCGAA 1 cut(s) 317
SgrBI CCGCGG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 4 cut(s) 169, 202, 537, 668
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 2 cut(s) 118, 431
StyD4I CCNGG 1 cut(s) 511
TaaI ACNGT 9 cut(s) 242, 460, 545, 651, 712, 726, 795, 831, 850
TaiI ACGT 5 cut(s) 282, 316, 387, 569, 887
TaqI TCGA 6 cut(s) 103, 317, 398, 555, 563, 736
TasI AATT 4 cut(s) 169, 202, 537, 668
TatI WGTACW 2 cut(s) 401, 712
TauI GCSGC 4 cut(s) 87, 467, 485, 488
TfiI GAWTC 2 cut(s) 78, 657
Tru1I TTAA 3 cut(s) 135, 177, 839
Tru9I TTAA 3 cut(s) 135, 177, 839
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 2 cut(s) 224, 475
TseI GCWGC 2 cut(s) 420, 718
Tsp45I GTSAC 2 cut(s) 224, 475
TspDTI ATGAA 2 cut(s) 41, 548
TspGWI ACGGA 4 cut(s) 45, 263, 324, 463
TspRI CASTG 1 cut(s) 834
Tth111I GACNNNGTC 1 cut(s) 886
XapI RAATTY 2 cut(s) 537, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 766
XspI CTAG 1 cut(s) 417
ZraI GACGTC 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.