RLG00000005026

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015444
Physical Location & Seq
Forward (+)
64428517 .. 64430998
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005026

Sequence Viewer

Length: 972 bp
ATGCAAGACATTCAGTCGATCGGGGGGAGGTTCTTTGGCGGCGCCGGAGGCGGAGACCGGAGGCTACGGCCGCACCACAACCAGAACCACCAGGCCTTGAAGTGCCCTAGGTGCGACTCGCTCAACACCAAGTTCTGCTACTACAACAACTACAACCTCTCTCAGCCTCGCCACTTCTGCAAGAGCTGCCGCCGCTACTGGACCAAAGGCGGCGTCCTCCGCAACGTCCCCGTCGGCGGAGGCTGCCGGAAAACGAAGCGTTCCAAGCCGAAGTCTTCTTCTTCTTCATCGCCGGCCTCACCGCCGCAGCAGCCGAACAGCGAGCGCAGAGCCAGCTCCAATTCCAGCAGCGAGAGCTCGAGCCTCACGAACACGGCCACCGCAACGGCGACCGAGGCGGTCTCGGCGCCGTCTTCGACGTCCTCGGCTTCCAATCTTCTCAACATTCCTCGTCCCGAGTCCAAATTCTTCGTTTCCCCAAACACGAATTTCGAGGCGGCTGGAGCCACCATGCTCGAACAGAGCGCCACGGAAATTGGAATGTTCTCGGATATTGGGAGCTTCACGAGCTTGATCACGGCCTCCTCGAACGACGTGCCGTTCGGTTTCACCAACATCAACGACATTTCCGTCTCGCCGTTCAGGCCAAACCAACTGGCGGCCGGTCATCTAAATCAGGTGCAGCAAAACCAGTGGGAGCAGCAACACAACCAGAATAACCAGGAACTGAAGATACCGGAGATCACCGGAGGAATGCTGGATCAGACGGCTCAGGTGGATTTATCAGTGTTCCAAACCAAAATCAACGGCGGAGGAGGAGGAGGGTTTGGGTCGCTGGATTGGCAGCCGGCCGGTGATCAAGGTCTGTTTGATCTCCCCAACACCGTTGATCAAGCATACTGGAGTCAGGGTTTCGAGATTCATGTTCCGGTGGAAATGGGTAATTGGGAGCAAACATACAGTGGTAACTAA

Protein Analysis

324

Amino Acids

35.02

Weight (kDa)

7.66

Isoelectric Point (pI)

60.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 31 - 87 1.2e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60850
fragaria_vesca FvH4_5g13390
malus_domestica MD06G1205900.v1.1 MD14G1216800.v1.1
prunus_persica Prupe.5G210200_v2.0.a1
pyrus_communis pycom06g18370 pycom14g17960
rosa_chinensis RchiOBHm_Chr7g0183751
rosa_laevigata RLG00000005026
rosa_multiflora Rmu_sc0003610.1_g000031
rosa_rugosa Rorug06G0460000
rosa_samantha Rh7AG060700 Rh7BG060800 Rh7CG062400 Rh7DG060800
rosa_wichuraiana Rw7G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 629
AatII GACGTC 1 cut(s) 422
AccB1I GGYRCC 2 cut(s) 41, 406
AclWI GGATC 1 cut(s) 768
AcoI YGGCCR 4 cut(s) 68, 375, 660, 849
AcsI RAATTY 2 cut(s) 464, 487
AcuI CTGAAG 1 cut(s) 749
AcyI GRCGYC 4 cut(s) 42, 213, 407, 419
AfiI CCNNNNNNNGG 2 cut(s) 236, 658
AgsI TTSAA 1 cut(s) 100
AhdI GACNNNNNGTC 1 cut(s) 13
AjiI CACGTC 1 cut(s) 595
AjnI CCWGG 2 cut(s) 90, 720
AluBI AGCT 5 cut(s) 186, 336, 357, 561, 570
AluI AGCT 5 cut(s) 186, 336, 357, 561, 570
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 3 cut(s) 48, 406, 637
AlwI GGATC 1 cut(s) 768
AlwNI CAGNNNCTG 1 cut(s) 727
Ama87I CYCGRG 2 cut(s) 358, 455
AoxI GGCC 8 cut(s) 68, 93, 294, 375, 579, 644, 660, 849
ApeKI GCWGC 8 cut(s) 186, 243, 307, 310, 348, 682, 700, 844
ApoI RAATTY 2 cut(s) 464, 487
ArsI GACNNNNNNTTYG 4 cut(s) 198, 230, 584, 616
AspA2I CCTAGG 1 cut(s) 107
AspLEI GCGC 4 cut(s) 44, 327, 409, 527
AspS9I GGNCC 1 cut(s) 201
AsuHPI GGTGA 4 cut(s) 291, 601, 736, 866
AvaI CYCGRG 2 cut(s) 358, 455
AvaII GGWCC 1 cut(s) 201
AvrII CCTAGG 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 107
BanI GGYRCC 2 cut(s) 41, 406
BanII GRGCYC 1 cut(s) 359
BauI CACGAG 1 cut(s) 565
BbsI GAAGAC 2 cut(s) 267, 405
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 8 cut(s) 173, 230, 319, 322, 360, 694, 712, 856
BceAI ACGGC 9 cut(s) 83, 390, 394, 402, 583, 594, 622, 783, 823
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 2 cut(s) 92, 722
BclI TGATCA 3 cut(s) 573, 856, 889
BcoDI GTCTC 3 cut(s) 48, 406, 637
BfaI CTAG 1 cut(s) 108
BfoI RGCGCY 3 cut(s) 45, 410, 528
BglI GCCNNNNNGGC 1 cut(s) 643
BlnI CCTAGG 1 cut(s) 107
Bme1390I CCNGG 2 cut(s) 92, 722
Bme18I GGWCC 1 cut(s) 201
BmeRI GACNNNNNGTC 1 cut(s) 13
BmeT110I CYCGRG 2 cut(s) 358, 455
BmgBI CACGTC 1 cut(s) 595
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 3 cut(s) 43, 408, 505
BmrFI CCNGG 2 cut(s) 92, 722
BpiI GAAGAC 2 cut(s) 267, 405
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 2 cut(s) 522, 922
Bpu10I CCTNAGC 1 cut(s) 771
BsaHI GRCGYC 4 cut(s) 42, 213, 407, 419
BsaI GGTCTC 2 cut(s) 48, 406
BsaJI CCNNGG 4 cut(s) 107, 393, 423, 528
BsaWI WCCGGW 4 cut(s) 57, 736, 746, 928
BsaXI ACNNNNNCTCC 2 cut(s) 895, 925
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 658
Bse118I RCCGGY 4 cut(s) 292, 662, 847, 851
Bse1I ACTGG 4 cut(s) 203, 660, 691, 905
BseBI CCWGG 2 cut(s) 92, 722
BseDI CCNNGG 4 cut(s) 107, 393, 423, 528
BseLI CCNNNNNNNGG 2 cut(s) 236, 658
BseMII CTCAG 2 cut(s) 176, 785
BseNI ACTGG 4 cut(s) 203, 660, 691, 905
BseRI GAGGAG 4 cut(s) 574, 828, 831, 834
BseSI GKGCMC 1 cut(s) 107
BseX3I CGGCCG 3 cut(s) 68, 660, 849
BseXI GCAGC 8 cut(s) 173, 230, 319, 322, 360, 694, 712, 856
BsgI GTGCAG 1 cut(s) 701
Bsh1285I CGRYCG 5 cut(s) 21, 71, 393, 663, 852
BshFI GGCC 8 cut(s) 70, 95, 296, 377, 581, 646, 662, 851
BshNI GGYRCC 2 cut(s) 41, 406
BsiEI CGRYCG 5 cut(s) 21, 71, 393, 663, 852
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 2 cut(s) 358, 455
BslFI GGGAC 2 cut(s) 212, 438
BslI CCNNNNNNNGG 2 cut(s) 236, 658
BsmAI GTCTC 3 cut(s) 48, 406, 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 212, 438
BsmI GAATGC 1 cut(s) 759
BsnI GGCC 8 cut(s) 70, 95, 296, 377, 581, 646, 662, 851
Bso31I GGTCTC 2 cut(s) 48, 406
BsoBI CYCGRG 2 cut(s) 358, 455
Bsp1286I GDGCHC 2 cut(s) 107, 359
Bsp143I GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
BspANI GGCC 8 cut(s) 70, 95, 296, 377, 581, 646, 662, 851
BspCNI CTCAG 2 cut(s) 175, 784
BspLI GGNNCC 3 cut(s) 43, 408, 505
BspPI GGATC 1 cut(s) 768
BspT107I GGYRCC 2 cut(s) 41, 406
BspTNI GGTCTC 2 cut(s) 48, 406
BsrFI RCCGGY 4 cut(s) 292, 662, 847, 851
BsrI ACTGG 4 cut(s) 203, 660, 691, 905
BssAI RCCGGY 4 cut(s) 292, 662, 847, 851
BssECI CCNNGG 4 cut(s) 107, 393, 423, 528
BssMI GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
BssNI GRCGYC 4 cut(s) 42, 213, 407, 419
BssSI CACGAG 1 cut(s) 565
BssT1I CCWWGG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 565
Bst2UI CCWGG 2 cut(s) 92, 722
Bst4CI ACNGT 2 cut(s) 886, 962
BstACI GRCGYC 4 cut(s) 42, 213, 407, 419
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 4 cut(s) 294, 323, 334, 849
BstDEI CTNAG 2 cut(s) 162, 771
BstDSI CCRYGG 1 cut(s) 528
BstH2I RGCGCY 3 cut(s) 45, 410, 528
BstHHI GCGC 4 cut(s) 44, 327, 409, 527
BstKTI GATC 7 cut(s) 21, 576, 744, 763, 859, 874, 892
BstMAI GTCTC 3 cut(s) 48, 406, 637
BstMBI GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
BstMCI CGRYCG 5 cut(s) 21, 71, 393, 663, 852
BstNI CCWGG 2 cut(s) 92, 722
BstSCI CCNGG 2 cut(s) 90, 720
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 8 cut(s) 173, 230, 319, 322, 360, 694, 712, 856
BstV2I GAAGAC 2 cut(s) 267, 405
BstZI CGGCCG 3 cut(s) 68, 660, 849
BsuRI GGCC 8 cut(s) 70, 95, 296, 377, 581, 646, 662, 851
BtgI CCRYGG 1 cut(s) 528
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 698, 792, 967
Cac8I GCNNGC 4 cut(s) 294, 323, 334, 849
CaiI CAGNNNCTG 1 cut(s) 727
CfoI GCGC 4 cut(s) 44, 327, 409, 527
Cfr10I RCCGGY 4 cut(s) 292, 662, 847, 851
Cfr13I GGNCC 1 cut(s) 201
CseI GACGC 1 cut(s) 202
CspCI CAANNNNNGTGG 4 cut(s) 517, 552, 674, 709
CviAII CATG 2 cut(s) 511, 923
DdeI CTNAG 2 cut(s) 162, 771
DinI GGCGCC 2 cut(s) 43, 408
DpnI GATC 7 cut(s) 20, 575, 743, 762, 858, 873, 891
DpnII GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
DrdI GACNNNNNNGTC 1 cut(s) 629
DriI GACNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 629
EaeI YGGCCR 4 cut(s) 68, 375, 660, 849
EagI CGGCCG 3 cut(s) 68, 660, 849
Eam1105I GACNNNNNGTC 1 cut(s) 13
EciI GGCGGA 3 cut(s) 66, 252, 825
Ecl136II GAGCTC 1 cut(s) 357
EclXI CGGCCG 3 cut(s) 68, 660, 849
Eco130I CCWWGG 1 cut(s) 107
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 2 cut(s) 48, 406
Eco47I GGWCC 1 cut(s) 201
Eco52I CGGCCG 3 cut(s) 68, 660, 849
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 749
Eco88I CYCGRG 2 cut(s) 358, 455
EcoICRI GAGCTC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 90, 720
EcoT14I CCWWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 359
EgeI GGCGCC 2 cut(s) 43, 408
EheI GGCGCC 2 cut(s) 43, 408
ErhI CCWWGG 1 cut(s) 107
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 2 cut(s) 514, 926
FaiI YATR 4 cut(s) 512, 898, 924, 958
FaqI GGGAC 2 cut(s) 212, 438
FatI CATG 2 cut(s) 510, 922
FbaI TGATCA 3 cut(s) 573, 856, 889
FriOI GRGCYC 1 cut(s) 359
FspBI CTAG 1 cut(s) 108
GlaI GCGC 4 cut(s) 43, 326, 408, 526
GsuI CTGGAG 2 cut(s) 522, 922
HaeII RGCGCY 3 cut(s) 45, 410, 528
HaeIII GGCC 8 cut(s) 70, 95, 296, 377, 581, 646, 662, 851
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 44, 327, 409, 527
Hin1I GRCGYC 4 cut(s) 42, 213, 407, 419
Hin1II CATG 2 cut(s) 514, 926
Hin6I GCGC 4 cut(s) 42, 325, 407, 525
HinP1I GCGC 4 cut(s) 42, 325, 407, 525
HinfI GANTC 4 cut(s) 116, 458, 904, 919
HphI GGTGA 4 cut(s) 291, 601, 736, 866
Hpy188I TCNGA 2 cut(s) 550, 765
Hpy188III TCNNGA 4 cut(s) 367, 455, 565, 916
Hpy99I CGWCG 3 cut(s) 236, 421, 596
HpyCH4III ACNGT 2 cut(s) 886, 962
HpyCH4IV ACGT 3 cut(s) 225, 419, 594
HpyCH4V TGCA 3 cut(s) 4, 180, 682
HpyF3I CTNAG 2 cut(s) 162, 771
HpySE526I ACGT 3 cut(s) 225, 419, 594
Hsp92I GRCGYC 4 cut(s) 42, 213, 407, 419
Hsp92II CATG 2 cut(s) 514, 926
HspAI GCGC 4 cut(s) 42, 325, 407, 525
KasI GGCGCC 2 cut(s) 41, 406
KroI GCCGGC 2 cut(s) 292, 847
KroNI GCCGGC 2 cut(s) 294, 849
Ksp22I TGATCA 3 cut(s) 573, 856, 889
Kzo9I GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
LmnI GCTCC 5 cut(s) 341, 503, 558, 697, 949
Lsp1109I GCAGC 8 cut(s) 173, 230, 319, 322, 360, 694, 712, 856
MaeI CTAG 1 cut(s) 108
MaeII ACGT 3 cut(s) 225, 419, 594
MaeIII GTNAC 1 cut(s) 965
MalI GATC 7 cut(s) 20, 575, 743, 762, 858, 873, 891
MboI GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
MboII GAAGA 8 cut(s) 267, 270, 273, 276, 405, 428, 460, 742
MhlI GDGCHC 2 cut(s) 107, 359
MluCI AATT 5 cut(s) 340, 464, 487, 534, 943
Mly113I GGCGCC 2 cut(s) 42, 407
MlyI GAGTC 3 cut(s) 110, 467, 913
MroNI GCCGGC 2 cut(s) 292, 847
MspR9I CCNGG 2 cut(s) 92, 722
Mva1269I GAATGC 1 cut(s) 759
MvaI CCWGG 2 cut(s) 92, 722
NaeI GCCGGC 2 cut(s) 294, 849
NarI GGCGCC 2 cut(s) 42, 407
NdeII GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
NgoMIV GCCGGC 2 cut(s) 292, 847
NlaIII CATG 2 cut(s) 514, 926
NlaIV GGNNCC 3 cut(s) 43, 408, 505
NmeAIII GCCGAG 2 cut(s) 383, 404
PaeR7I CTCGAG 1 cut(s) 358
PceI AGGCCT 1 cut(s) 95
PcsI WCGNNNNNNNCGW 3 cut(s) 365, 584, 627
PctI GAATGC 1 cut(s) 759
PdiI GCCGGC 2 cut(s) 294, 849
PfeI GAWTC 1 cut(s) 919
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 3 cut(s) 110, 466, 912
PluTI GGCGCC 2 cut(s) 45, 410
PpsI GAGTC 3 cut(s) 110, 466, 912
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 2 cut(s) 90, 720
PspGI CCWGG 2 cut(s) 90, 720
PspN4I GGNNCC 3 cut(s) 43, 408, 505
PspPI GGNCC 1 cut(s) 201
PspXI VCTCGAGB 1 cut(s) 358
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PstNI CAGNNNCTG 1 cut(s) 727
PvuI CGATCG 1 cut(s) 21
SacI GAGCTC 1 cut(s) 359
Sau3AI GATC 7 cut(s) 18, 573, 741, 760, 856, 871, 889
Sau96I GGNCC 1 cut(s) 201
SchI GAGTC 3 cut(s) 110, 467, 913
ScrFI CCNGG 2 cut(s) 92, 722
SduI GDGCHC 2 cut(s) 107, 359
SfoI GGCGCC 2 cut(s) 43, 408
Sfr274I CTCGAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 201
SlaI CTCGAG 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 5 cut(s) 340, 464, 487, 534, 943
SseBI AGGCCT 1 cut(s) 95
SspDI GGCGCC 2 cut(s) 41, 406
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 359
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 90, 720
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 886, 962
TaiI ACGT 3 cut(s) 228, 422, 597
TaqI TCGA 7 cut(s) 17, 359, 416, 492, 516, 587, 915
TaqII GACCGA 1 cut(s) 407
TasI AATT 5 cut(s) 340, 464, 487, 534, 943
TauI GCSGC 8 cut(s) 42, 73, 192, 195, 213, 307, 500, 662
TfiI GAWTC 1 cut(s) 919
TscAI CASTG 3 cut(s) 698, 792, 967
TseI GCWGC 8 cut(s) 186, 243, 307, 310, 348, 682, 700, 844
TspDTI ATGAA 2 cut(s) 276, 911
TspGWI ACGGA 2 cut(s) 545, 619
TspRI CASTG 3 cut(s) 698, 792, 967
VpaK11BI GGWCC 1 cut(s) 201
XapI RAATTY 2 cut(s) 464, 487
XhoI CTCGAG 1 cut(s) 358
XmaJI CCTAGG 1 cut(s) 107
XspI CTAG 1 cut(s) 108
ZraI GACGTC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.