AT5G62270

BEST Arabidopsis thaliana protein match is mucin-related (TAIR AT2G02880.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011235
Physical Location & Seq
Forward (+)
25011957 .. 25014812
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62270.2

Sequence Viewer

Length: 1152 bp
ATGTATAACCTTAGTAGGATCATTTATCGTTTCTCCTCGGTTTCACTTAACCCTAGCACGAGAGCATCTGGGTTTCTACTTGAGAACGCTTCATCAAAAATCTTACAATCTGCTACGAATCGAGCATTTTCTGCAAAATCAGGATCTGATGGAGTTGGAGGCGATGATAACGGCTGGAATATTTCAACAGGAGGATCATTTGGTGGTACAGGATCTGCTGATCTTGATTGGGACAATAAATCTATGTGGTCTACTGGTTTAACTAAGGAGCATTTCGATGGTGTCTCCGTTGGTCGTCAGAAGAACGCGGCGAATCCTTCTTCTGATAACACCCCTTCTGATTCGGGTGATGTAATGAGCAAATTGGGTCCAAAGGAGGTAGCTTTGGTTAATGAAATGAATGAGTATGATGATTTGCTTAAAGAGATTGAGCAAGATAATAGGCAAGGCAGGGCTTTTGTTGATGGGATTAAACAGAGAATGATGGAGATCAGTGTGTTGTTGAAGCAAGTTAAAGAGCCAGGTGCTAGAGGGTCTTATCTTAAGGACTCTGAGAAGACTGAGATGTATAGATTGCACAAAGAGAATCCTGAGGTGTATACTATTGAGAGGCTTGCTAAGGATTATAGGATTATGAGGCAGCGTGTTCACGCCATTCTTTTTCTTAAAGAAGATGAAGAAGAAGAGGAGAGGAAGCTTGGTCGTCCTTTGGATGATTCTGTTGACCGTTTGCTTGATGAGTACCCTGAGTTCTTCATTTCGCATGACCGGGAATTTCATGTGGCCTCACTTAATTACAAACCCGACTTCAAGGTCATGCCAGAAGGATGGGATGGTACAATCAAAGATATGGATGAAGTCCATTATGAGATCTCAAAGAAAGAGGATGACATGCTTTACGAAGAATTTGTCCGAAGGTTTGAGTTCAACAAAATGAAATGGAGAGGAGAAGTGATGTGCCACAAGTACAGCAGGAGACGTTCATCAGAGGGATGGAAAATCACGGTTGAGAAATTGGGTGCTAAGGGCAAACGTGGAGCCGGAGGTGGCTGGAAGTTTATGAGTCTTCCAGATGGATCAAGCCGACCACTCAACGAAATGGAGAAGGTCTATGTTAAGCGTGAAACCCCACTACGTCGCCGTAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

383

Amino Acids

43.94

Weight (kDa)

6.45

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-L20 PF12824 163 - 229 4.3e-06 Mitochondrial ribosomal protein subunit L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 812
AccI GTMKAC 2 cut(s) 251, 599
AccII CGCG 1 cut(s) 308
AciI CCGC 1 cut(s) 308
AclWI GGATC 5 cut(s) 26, 151, 202, 220, 1084
AcsI RAATTY 2 cut(s) 773, 905
AfaI GTAC 4 cut(s) 208, 743, 838, 968
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 4 cut(s) 186, 505, 811, 928
AjnI CCWGG 1 cut(s) 520
AluBI AGCT 2 cut(s) 383, 697
AluI AGCT 2 cut(s) 383, 697
Alw26I GTCTC 2 cut(s) 289, 970
AlwI GGATC 5 cut(s) 26, 151, 202, 220, 1084
AlwNI CAGNNNCTG 2 cut(s) 146, 215
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 1 cut(s) 640
ApoI RAATTY 2 cut(s) 773, 905
ArsI GACNNNNNNTTYG 2 cut(s) 894, 926
AspS9I GGNCC 1 cut(s) 368
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 591
BauI CACGAG 1 cut(s) 58
BbsI GAAGAC 2 cut(s) 563, 1058
BbvI GCAGC 1 cut(s) 652
BccI CCATC 8 cut(s) 143, 272, 458, 478, 822, 827, 987, 1067
BceAI ACGGC 2 cut(s) 187, 1125
BciT130I CCWGG 1 cut(s) 522
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 289, 970
BfaI CTAG 3 cut(s) 54, 528, 1150
BfrI CTTAAG 1 cut(s) 542
BglII AGATCT 2 cut(s) 870, 1146
BisI GCNGC 2 cut(s) 309, 641
BlsI GCNGC 2 cut(s) 310, 642
Bme1390I CCNGG 2 cut(s) 522, 770
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 369, 1039
BmrFI CCNGG 2 cut(s) 522, 770
BmsI GCATC 1 cut(s) 74
BpiI GAAGAC 2 cut(s) 563, 1058
Bpu10I CCTNAGC 2 cut(s) 618, 1023
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 770
BsaBI GATNNNNATC 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 259
Bse21I CCTNAGG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 488
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 6 cut(s) 718, 833, 838, 859, 892, 998
BseJI GATNNNNATC 1 cut(s) 488
BseMII CTCAG 4 cut(s) 543, 552, 582, 738
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 3 cut(s) 25, 701, 960
BseXI GCAGC 1 cut(s) 652
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 785
BsiSI CCGG 2 cut(s) 769, 1041
BslFI GGGAC 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 289, 970
BsmBI CGTCTC 1 cut(s) 970
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 1 cut(s) 785
Bsp143I GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
BspACI CCGC 1 cut(s) 308
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 4 cut(s) 544, 553, 583, 739
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 2 cut(s) 369, 1039
BspPI GGATC 5 cut(s) 26, 151, 202, 220, 1084
BspTI CTTAAG 1 cut(s) 542
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
BssNAI GTATAC 1 cut(s) 600
BssSI CACGAG 1 cut(s) 58
Bst1107I GTATAC 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 2 cut(s) 728, 1006
Bst6I CTCTTC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 615
BstDEI CTNAG 8 cut(s) 11, 264, 552, 561, 591, 618, 747, 1023
BstF5I GGATG 6 cut(s) 718, 833, 838, 859, 892, 998
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 9 cut(s) 21, 146, 197, 215, 223, 492, 873, 1079, 1149
BstMAI GTCTC 2 cut(s) 289, 970
BstMBI GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 1 cut(s) 895
BstSCI CCNGG 2 cut(s) 520, 768
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 652
BstV2I GAAGAC 2 cut(s) 563, 1058
BstX2I RGATCY 4 cut(s) 143, 212, 870, 1146
BstXI CCANNNNNNTGG 1 cut(s) 828
BstYI RGATCY 4 cut(s) 143, 212, 870, 1146
BstZ17I GTATAC 1 cut(s) 600
Bsu36I CCTNAGG 1 cut(s) 591
BsuRI GGCC 1 cut(s) 785
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 6 cut(s) 718, 833, 838, 859, 892, 998
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 1 cut(s) 615
CaiI CAGNNNCTG 2 cut(s) 146, 215
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 4 cut(s) 207, 742, 837, 967
CviAII CATG 4 cut(s) 764, 779, 817, 892
CviQI GTAC 4 cut(s) 207, 742, 837, 967
DdeI CTNAG 8 cut(s) 11, 264, 552, 561, 591, 618, 747, 1023
DpnI GATC 9 cut(s) 20, 145, 196, 214, 222, 491, 872, 1078, 1148
DpnII GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
DrdI GACNNNNNNGTC 1 cut(s) 812
DseDI GACNNNNNNGTC 1 cut(s) 812
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Eco47I GGWCC 1 cut(s) 368
Eco81I CCTNAGG 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 520
Esp3I CGTCTC 1 cut(s) 970
FaeI CATG 4 cut(s) 767, 782, 820, 895
FaqI GGGAC 1 cut(s) 245
FatI CATG 4 cut(s) 763, 778, 816, 891
FblI GTMKAC 2 cut(s) 251, 599
Fnu4HI GCNGC 2 cut(s) 309, 641
FokI GGATG 6 cut(s) 725, 840, 845, 866, 899, 1005
Fsp4HI GCNGC 2 cut(s) 309, 641
FspBI CTAG 3 cut(s) 54, 528, 1150
GluI GCNGC 2 cut(s) 309, 641
HaeIII GGCC 1 cut(s) 785
HapII CCGG 2 cut(s) 769, 1041
Hin1II CATG 4 cut(s) 767, 782, 820, 895
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 7 cut(s) 118, 313, 341, 548, 586, 716, 1063
HpaII CCGG 2 cut(s) 769, 1041
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 4 cut(s) 252, 600, 649, 724
Hpy188I TCNGA 7 cut(s) 148, 300, 325, 340, 553, 914, 988
Hpy188III TCNNGA 4 cut(s) 141, 224, 590, 1070
Hpy8I GTNNAC 4 cut(s) 252, 600, 649, 724
Hpy99I CGWCG 1 cut(s) 1140
HpyAV CCTTC 5 cut(s) 327, 345, 818, 909, 1099
HpyCH4III ACNGT 2 cut(s) 728, 1006
HpyCH4IV ACGT 3 cut(s) 979, 1033, 1135
HpyCH4V TGCA 2 cut(s) 134, 577
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 8 cut(s) 11, 264, 552, 561, 591, 618, 747, 1023
HpySE526I ACGT 3 cut(s) 979, 1033, 1135
Hsp92II CATG 4 cut(s) 767, 782, 820, 895
Kzo9I GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
LmnI GCTCC 2 cut(s) 268, 1037
Lsp1109I GCAGC 1 cut(s) 652
LweI GCATC 1 cut(s) 74
MaeI CTAG 3 cut(s) 54, 528, 1150
MaeII ACGT 3 cut(s) 979, 1033, 1135
MalI GATC 9 cut(s) 20, 145, 196, 214, 222, 491, 872, 1078, 1148
MboI GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
MflI RGATCY 4 cut(s) 143, 212, 870, 1146
MluCI AATT 5 cut(s) 362, 773, 793, 905, 1013
MlyI GAGTC 2 cut(s) 542, 1072
MmeI TCCRAC 1 cut(s) 136
MslI CAYNNNNRTG 1 cut(s) 276
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 2 cut(s) 769, 1041
MspR9I CCNGG 2 cut(s) 522, 770
MvaI CCWGG 1 cut(s) 522
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 131
NciI CCSGG 1 cut(s) 770
NdeII GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
NlaIII CATG 4 cut(s) 767, 782, 820, 895
NlaIV GGNNCC 2 cut(s) 369, 1039
NspI RCATGY 1 cut(s) 895
PfeI GAWTC 5 cut(s) 118, 313, 341, 586, 716
PkrI GCNGC 2 cut(s) 310, 642
PleI GAGTC 2 cut(s) 542, 1071
PpsI GAGTC 2 cut(s) 542, 1071
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 369, 1039
PspPI GGNCC 1 cut(s) 368
PsrI GAACNNNNNNTAC 2 cut(s) 734, 766
PstNI CAGNNNCTG 2 cut(s) 146, 215
PsuI RGATCY 4 cut(s) 143, 212, 870, 1146
RsaI GTAC 4 cut(s) 208, 743, 838, 968
RsaNI GTAC 4 cut(s) 207, 742, 837, 967
RseI CAYNNNNRTG 1 cut(s) 276
SatI GCNGC 2 cut(s) 309, 641
Sau3AI GATC 9 cut(s) 18, 143, 194, 212, 220, 489, 870, 1076, 1146
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 2 cut(s) 542, 1072
ScrFI CCNGG 2 cut(s) 522, 770
SfaNI GCATC 1 cut(s) 74
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 2 cut(s) 80, 542
SmoI CTYRAG 2 cut(s) 80, 542
Sse9I AATT 5 cut(s) 362, 773, 793, 905, 1013
SsiI CCGC 1 cut(s) 308
SspI AATATT 1 cut(s) 181
SspMI CTAG 3 cut(s) 54, 528, 1150
StyD4I CCNGG 2 cut(s) 520, 768
TaaI ACNGT 2 cut(s) 728, 1006
TaiI ACGT 3 cut(s) 982, 1036, 1138
TaqI TCGA 2 cut(s) 121, 276
TasI AATT 5 cut(s) 362, 773, 793, 905, 1013
TatI WGTACW 1 cut(s) 966
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 5 cut(s) 118, 313, 341, 586, 716
TscAI CASTG 1 cut(s) 499
TseI GCWGC 1 cut(s) 640
TspDTI ATGAA 9 cut(s) 81, 408, 413, 690, 745, 767, 870, 950, 972
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 773, 905
XceI RCATGY 1 cut(s) 895
XmiI GTMKAC 2 cut(s) 251, 599
XspI CTAG 3 cut(s) 54, 528, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.