Rw5G030140

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011235
Physical Location & Seq
Reverse (-)
47497838 .. 47503971
6134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030140.1

Sequence Viewer

Length: 1257 bp
ATGTTCTTGGGATATTCTGCACCTGTGGGTTGGCAAATTAGATTACTTCCCGGAGCTATGCAGAATCTGCACCGTCTTATATCTCGTCTTTCCTCAACTTCAATCTGCAAAAGCACGACTCCGACAAAGTTTCTGGGGGAGAAGAGCAACGCCAGTTTGGGAAACAATGGTGTTGAAGCTTGTAAGCCTATGAGCTCCCGATTTTTTTCTTTTCAATCTGGTGGTGATGGTCAAAATGATGGTAACAGTGGGTGGGATAAACCTGCGGCGGGAACATTTGGCAATAATGCATCAGATAATTTGGGGTGGGATTCTACGTCCTCGTGGTCGCTTGGACTGACCAAGGAGCATTTTGATGGGGAGGTGGTGGGGCGACGAACGAGCGGAGTTGAGACTCCCCAGTCTGCAGTCATCTCTGGTTTGCAAGAGATTGAGGATAGAATAAAGGAATTGGAGGCAGAGAACCGGAAAAGTAAGAGATTTGTTGATGGATGGGGAGAGAGATTGAAAGAGATAAGTGTGCTGATGAAGCAAGTGCGTGAACCGGGTGCAAGAGGGTCTTATCTCAAGGACTCGGAAAAGGCTGAGATGTATCGATTGCACAAGGAAAACCCTGATGTTTACACTGTTGAGCGCCTGGCCAAGGATTATAGGATTATGAGGCAGAGAGTGCATGCCATTCTTTGGCTGAAGGAGCTTCAGGAGGAAGAGGAGAAGAAGCTTGGTCGTCCTTATGATTCTGTTGAGCTCCTGCTTGACAGCTTTCCTGAGATTCTATGCAAATTTTATGTTTATGATATAGGTTTGCTCATTGAGATTTTTTTTGGTTCCTGTGGGGCTAACACTTTTCCTCATTCAGATTTTTTCAATTCCATGACCGTGGAATTCCATGTTGCATCACTTCCTTACAAACCTGACTTCAAGGTTATGCCAGAGGGTTGGGATGATGAGATGCTGTATCAAGAATTTGTCCAGAGGATGAACTTCAACAAAATGAAAATGAGAGGAGAGGTTTTCCACCACAAGTATAGTCGTCGTCGCTCTGCAGATGGGTGGAATTTCACGGTGGAGAAAATGGGACCCAAAGGAAAGCGTGGAGATGGTGGTGGCTGGAAGTTTGTCAGCCTGCCAGATGGTTCGAGCCGACCACTGAATGAAAAGGAGAAAATGTATGTGAAACGAGAAACTCCACGCCATCGACAACAGCGTGTGTCTGAAGGGACCAAAGTTGATATATATACACCAGCATCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

418

Amino Acids

47.68

Weight (kDa)

8.77

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-L20 PF12824 160 - 237 3.5e-06 Mitochondrial ribosomal protein subunit L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 684
AccBSI CCGCTC 1 cut(s) 384
AciI CCGC 3 cut(s) 266, 269, 384
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 4 cut(s) 782, 884, 965, 1057
AcuI CTGAAG 3 cut(s) 683, 710, 1236
AfiI CCNNNNNNNGG 3 cut(s) 269, 643, 684
AgsI TTSAA 7 cut(s) 102, 176, 215, 508, 868, 922, 988
AjnI CCWGG 1 cut(s) 636
AluBI AGCT 7 cut(s) 56, 179, 195, 697, 721, 748, 762
AluI AGCT 7 cut(s) 56, 179, 195, 697, 721, 748, 762
Alw21I GWGCWC 2 cut(s) 197, 750
Alw26I GTCTC 1 cut(s) 386
AlwNI CAGNNNCTG 1 cut(s) 67
AoxI GGCC 1 cut(s) 639
ApoI RAATTY 4 cut(s) 782, 884, 965, 1057
AspLEI GCGC 1 cut(s) 636
AspS9I GGNCC 2 cut(s) 1079, 1221
AsuC2I CCSGG 2 cut(s) 51, 546
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 2 cut(s) 1079, 1221
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 2 cut(s) 197, 750
BauI CACGAG 1 cut(s) 322
Bbv12I GWGCWC 2 cut(s) 197, 750
BccI CCATC 9 cut(s) 221, 233, 350, 482, 486, 1043, 1094, 1127, 1203
BciT130I CCWGG 1 cut(s) 638
BcnI CCSGG 2 cut(s) 51, 546
BcoDI GTCTC 1 cut(s) 386
BfmI CTRYAG 2 cut(s) 405, 1044
BfoI RGCGCY 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 3 cut(s) 51, 546, 638
Bme18I GGWCC 2 cut(s) 1079, 1221
BmgT120I GGNCC 2 cut(s) 1079, 1221
BmiI GGNNCC 4 cut(s) 829, 1080, 1081, 1222
BmrFI CCNGG 3 cut(s) 51, 546, 638
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 3 cut(s) 299, 905, 942
BmuI ACTGGG 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 551
BpuMI CCSGG 2 cut(s) 51, 546
Bsa29I ATCGAT 1 cut(s) 595
BsaJI CCNNGG 3 cut(s) 342, 642, 879
BsaWI WCCGGW 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 1154, 1184
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 643, 684
Bse1I ACTGG 2 cut(s) 153, 400
BseBI CCWGG 1 cut(s) 638
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 3 cut(s) 342, 642, 879
BseGI GGATG 3 cut(s) 497, 949, 984
BseLI CCNNNNNNNGG 3 cut(s) 269, 643, 684
BseMII CTCAG 2 cut(s) 576, 759
BseNI ACTGG 2 cut(s) 153, 400
BseRI GAGGAG 2 cut(s) 725, 1020
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 1 cut(s) 641
BshVI ATCGAT 1 cut(s) 595
BsiHKAI GWGCWC 2 cut(s) 197, 750
BsiSI CCGG 3 cut(s) 51, 466, 545
BslFI GGGAC 2 cut(s) 1092, 1234
BslI CCNNNNNNNGG 3 cut(s) 269, 643, 684
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 1092, 1234
BsnI GGCC 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 197, 750
BspACI CCGC 3 cut(s) 266, 269, 384
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 2 cut(s) 577, 760
BspDI ATCGAT 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 829, 1080, 1081, 1222
BspMAI CTGCAG 2 cut(s) 409, 1048
BspMI ACCTGC 1 cut(s) 271
BspQI GCTCTTC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 384
BsrI ACTGG 2 cut(s) 153, 400
BssECI CCNNGG 3 cut(s) 342, 642, 879
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 342, 642
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 5 cut(s) 74, 248, 628, 880, 1066
Bst6I CTCTTC 2 cut(s) 137, 702
BstAPI GCANNNNNTGC 2 cut(s) 67, 670
BstC8I GCNNGC 2 cut(s) 675, 1127
BstDEI CTNAG 2 cut(s) 585, 768
BstDSI CCRYGG 1 cut(s) 879
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 3 cut(s) 497, 949, 984
BstH2I RGCGCY 1 cut(s) 637
BstHHI GCGC 1 cut(s) 636
BstMAI GTCTC 1 cut(s) 386
BstMWI GCNNNNNNNGC 4 cut(s) 67, 529, 670, 694
BstNI CCWGG 1 cut(s) 638
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 49, 544, 636
BstSFI CTRYAG 2 cut(s) 405, 1044
BstXI CCANNNNNNTGG 2 cut(s) 880, 939
Bsu15I ATCGAT 1 cut(s) 595
BsuRI GGCC 1 cut(s) 641
BsuTUI ATCGAT 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 3 cut(s) 497, 949, 984
BtsIMutI CAGTG 3 cut(s) 253, 624, 1148
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 675, 1127
CaiI CAGNNNCTG 1 cut(s) 67
CfoI GCGC 1 cut(s) 636
Cfr13I GGNCC 2 cut(s) 1079, 1221
ClaI ATCGAT 1 cut(s) 595
CviAII CATG 3 cut(s) 674, 874, 890
DdeI CTNAG 2 cut(s) 585, 768
DrdI GACNNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 2 cut(s) 137, 702
EarI CTCTTC 2 cut(s) 137, 702
Ecl136II GAGCTC 2 cut(s) 195, 748
Eco130I CCWWGG 2 cut(s) 342, 642
Eco24I GRGCYC 2 cut(s) 197, 750
Eco47I GGWCC 2 cut(s) 1079, 1221
Eco53kI GAGCTC 2 cut(s) 195, 748
Eco57I CTGAAG 3 cut(s) 683, 710, 1236
EcoICRI GAGCTC 2 cut(s) 195, 748
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 884
EcoRII CCWGG 1 cut(s) 636
EcoT14I CCWWGG 2 cut(s) 342, 642
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 197, 750
ErhI CCWWGG 2 cut(s) 342, 642
FaeI CATG 3 cut(s) 677, 877, 893
FalI AAGNNNNNCTT 2 cut(s) 544, 576
FaqI GGGAC 2 cut(s) 1092, 1234
FatI CATG 3 cut(s) 673, 873, 889
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 3 cut(s) 504, 956, 991
FriOI GRGCYC 2 cut(s) 197, 750
Fsp4HI GCNGC 1 cut(s) 267
GlaI GCGC 1 cut(s) 635
GluI GCNGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 637
HaeIII GGCC 1 cut(s) 641
HapII CCGG 3 cut(s) 51, 466, 545
HhaI GCGC 1 cut(s) 636
Hin1II CATG 3 cut(s) 677, 877, 893
Hin6I GCGC 1 cut(s) 634
HinP1I GCGC 1 cut(s) 634
HindIII AAGCTT 2 cut(s) 177, 719
HinfI GANTC 7 cut(s) 64, 118, 311, 394, 572, 737, 772
HpaII CCGG 3 cut(s) 51, 466, 545
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 542, 622
Hpy188I TCNGA 5 cut(s) 123, 295, 577, 859, 1216
Hpy188III TCNNGA 5 cut(s) 198, 701, 767, 962, 973
Hpy8I GTNNAC 2 cut(s) 542, 622
Hpy99I CGWCG 3 cut(s) 378, 1038, 1041
HpyAV CCTTC 2 cut(s) 685, 1211
HpyCH4III ACNGT 5 cut(s) 74, 248, 628, 880, 1066
HpyCH4IV ACGT 1 cut(s) 317
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 529, 670, 694
HpyF3I CTNAG 2 cut(s) 585, 768
HpySE526I ACGT 1 cut(s) 317
Hsp92II CATG 3 cut(s) 677, 877, 893
HspAI GCGC 1 cut(s) 634
KflI GGGWCCC 1 cut(s) 1079
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 5 cut(s) 53, 200, 346, 694, 753
LweI GCATC 3 cut(s) 299, 905, 942
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 1 cut(s) 242
MbiI CCGCTC 1 cut(s) 384
MboII GAAGA 3 cut(s) 154, 719, 727
MhlI GDGCHC 2 cut(s) 197, 750
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 8 cut(s) 36, 298, 449, 782, 868, 884, 965, 1057
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 3 cut(s) 112, 388, 566
MmeI TCCRAC 1 cut(s) 146
Mox20I TGGCCA 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 292
MscI TGGCCA 1 cut(s) 641
MslI CAYNNNNRTG 2 cut(s) 354, 878
Msp20I TGGCCA 1 cut(s) 641
MspI CCGG 3 cut(s) 51, 466, 545
MspR9I CCNGG 3 cut(s) 51, 546, 638
MvaI CCWGG 1 cut(s) 638
MwoI GCNNNNNNNGC 4 cut(s) 67, 529, 670, 694
NciI CCSGG 2 cut(s) 51, 546
NlaIII CATG 3 cut(s) 677, 877, 893
NlaIV GGNNCC 4 cut(s) 829, 1080, 1081, 1222
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 677
PaeI GCATGC 1 cut(s) 677
PciSI GCTCTTC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 1204
PfeI GAWTC 4 cut(s) 64, 311, 737, 772
PflMI CCANNNNNTGG 1 cut(s) 684
PfoI TCCNGGA 1 cut(s) 49
PkrI GCNGC 1 cut(s) 268
PleI GAGTC 3 cut(s) 112, 388, 566
PpsI GAGTC 3 cut(s) 112, 388, 566
PpuMI RGGWCCY 1 cut(s) 1079
Psp124BI GAGCTC 2 cut(s) 197, 750
Psp5II RGGWCCY 1 cut(s) 1079
Psp6I CCWGG 1 cut(s) 636
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 4 cut(s) 829, 1080, 1081, 1222
PspPI GGNCC 2 cut(s) 1079, 1221
PspPPI RGGWCCY 1 cut(s) 1079
PstI CTGCAG 2 cut(s) 409, 1048
PstNI CAGNNNCTG 1 cut(s) 67
RseI CAYNNNNRTG 2 cut(s) 354, 878
SacI GAGCTC 2 cut(s) 197, 750
SapI GCTCTTC 1 cut(s) 137
SatI GCNGC 1 cut(s) 267
Sau96I GGNCC 2 cut(s) 1079, 1221
SchI GAGTC 3 cut(s) 112, 388, 566
ScrFI CCNGG 3 cut(s) 51, 546, 638
SduI GDGCHC 2 cut(s) 197, 750
SfaNI GCATC 3 cut(s) 299, 905, 942
SfcI CTRYAG 2 cut(s) 405, 1044
SinI GGWCC 2 cut(s) 1079, 1221
SmiMI CAYNNNNRTG 2 cut(s) 354, 878
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SphI GCATGC 1 cut(s) 677
Sse9I AATT 8 cut(s) 36, 298, 449, 782, 868, 884, 965, 1057
SsiI CCGC 3 cut(s) 266, 269, 384
SstI GAGCTC 2 cut(s) 197, 750
StyD4I CCNGG 3 cut(s) 49, 544, 636
StyI CCWWGG 2 cut(s) 342, 642
TaaI ACNGT 5 cut(s) 74, 248, 628, 880, 1066
TaiI ACGT 1 cut(s) 320
TaqI TCGA 3 cut(s) 595, 1139, 1198
TasI AATT 8 cut(s) 36, 298, 449, 782, 868, 884, 965, 1057
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 4 cut(s) 64, 311, 737, 772
TscAI CASTG 3 cut(s) 253, 631, 1155
TspDTI ATGAA 4 cut(s) 542, 995, 1010, 1170
TspRI CASTG 3 cut(s) 253, 631, 1155
Van91I CCANNNNNTGG 1 cut(s) 684
VpaK11BI GGWCC 2 cut(s) 1079, 1221
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 4 cut(s) 782, 884, 965, 1057
XceI RCATGY 1 cut(s) 677
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.