RLG00000034557

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011235
Physical Location & Seq
Forward (+)
44919894 .. 44923220
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034557

Sequence Viewer

Length: 1308 bp
ATGCCTGCGAGGGTTTGGGGTTTAGATGTTACCATCTCTCAAATAATCCAACTCATTCCTTCCACTTACAAATGCTTCGATTTCGATCCGATTGAAGATTCAATTTCTGTGGGCGCTGCTTCAATCGTCATTTCAGGATATTCTGCACCTGTGGGTTGGCAAATTAGATTACTTCCCGGAGCTATGCAGAATCTGCACCGTCTTATATCTCGTCTTTCCTCAACTTCAATCTGCAAATGCACGAGTGCGACAAAGTTTCTGGGGGAGAAGAGCAACGCCAGTTTGGGAAACAATGGTGTTGAAGCTTGCAAGCTTATGAGCTCCCGATTTTTTTCTTTTCAATCTGGTGGTGATGGTCAAAATGATGGTCACAGTGGGTGGGATAAACCTGCGCCGGGAACATTTGGCAATAATGCTTCAGATAATTTGGGGTGGGATTCTACGTCCTCGTGGTCGCTTGGACTGACCAAGGAGCATTTTGATGGGGAGGTGGTTGGGCGACGAACAAGCGGAGATGAGACTCCCCAGTCTGCAGTCATCTCTGGTTTGCAAGAGATTGAGGATAGAATAAAGGAATTGGAGGCAGAGAACCGGAAAAGTAAGAGATTTGTTGATGGATGGGGAGAGAGATTGAAAGAGATAAGTGTGTTGATGAAGCAAGTGCGCGAACCGGGTGCAAGAGGGTCTTATCTCAAGGACTCGGAAAAGGCTGAGATGTATCGGTTGCACAAGGAAAACCCTGATGTTTACACTATTGAGCGCCTGGCCAAGGATTATAGGATTATGAGGCAGAGAGTGCATGCCATCCTTTGGCTGAAGGAGCTTCAGGAGGAAGAGGAGAAGAAGCTTGGTCGTCCCTTAGATGATTCTGTTGAGCTCCTGCTTGACAGCTTTCCTGAATTTTTCAATTCCCATGACCGTGAATTCCATGTTGCATCACTTCCTTACAAACCTGACTTCAAGGTTATGCCAGAGGGTTGGGATGGTACCACCAGAGATTTGGATGAAGTCCACTATGAGATCTCCAAGAAGGAAGATGAGATGCTGTATCAAGAATTTGTCCAGAGGATGAACTTCAACAAAATGAAAATGAGAGGAGAGGTTTTCCACCACAAGTATAGTCGTCGTCGCTCAGCAGATGGGTGGAATTTCACGGTGGAGAAAATGGGACCCAAAGGAAAGCGTGGAGGTGGTGGTGGTTGGAAGTTTGTCAGCCTGCCAGATGGTTCGAGCCGACCACTGAATGAAAAGGAGAAAATGTATGTGAAACGAGAAACTCCACGCCATCGACGCAAGATCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

436

Amino Acids

49.58

Weight (kDa)

8.16

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-L20 PF12824 202 - 279 5.5e-06 Mitochondrial ribosomal protein subunit L20
Bot1p PF12298 230 - 334 9.6e-06 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 526
Acc36I ACCTGC 1 cut(s) 397
Acc65I GGTACC 1 cut(s) 986
AccB1I GGYRCC 1 cut(s) 986
AccB7I CCANNNNNTGG 1 cut(s) 810
AccII CGCG 1 cut(s) 666
AciI CCGC 1 cut(s) 510
AclWI GGATC 2 cut(s) 80, 1291
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 4 cut(s) 899, 923, 1055, 1147
AcuI CTGAAG 3 cut(s) 402, 809, 836
AfaI GTAC 1 cut(s) 988
AfiI CCNNNNNNNGG 3 cut(s) 395, 769, 810
AjnI CCWGG 1 cut(s) 762
AluBI AGCT 8 cut(s) 182, 305, 313, 321, 823, 847, 877, 891
AluI AGCT 8 cut(s) 182, 305, 313, 321, 823, 847, 877, 891
Alw21I GWGCWC 2 cut(s) 323, 879
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 2 cut(s) 80, 1291
AlwNI CAGNNNCTG 1 cut(s) 193
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 4 cut(s) 899, 923, 1055, 1147
ArsI GACNNNNNNTTYG 2 cut(s) 352, 384
Asp718I GGTACC 1 cut(s) 986
AspLEI GCGC 4 cut(s) 116, 394, 666, 762
AspS9I GGNCC 1 cut(s) 1169
AsuC2I CCSGG 3 cut(s) 177, 396, 672
AsuHPI GGTGA 1 cut(s) 362
AvaII GGWCC 1 cut(s) 1169
BalI TGGCCA 1 cut(s) 767
BanI GGYRCC 1 cut(s) 986
BanII GRGCYC 2 cut(s) 323, 879
BauI CACGAG 2 cut(s) 241, 448
Bbv12I GWGCWC 2 cut(s) 323, 879
BbvI GCAGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 1 cut(s) 764
BcnI CCSGG 3 cut(s) 177, 396, 672
BcoDI GTCTC 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 531
BfoI RGCGCY 2 cut(s) 117, 763
BfuAI ACCTGC 1 cut(s) 397
BglII AGATCT 1 cut(s) 1020
BisI GCNGC 1 cut(s) 117
BlpI GCTNAGC 1 cut(s) 1132
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 4 cut(s) 177, 396, 672, 764
Bme18I GGWCC 1 cut(s) 1169
BmgT120I GGNCC 1 cut(s) 1169
BmiI GGNNCC 3 cut(s) 988, 1170, 1171
BmrFI CCNGG 4 cut(s) 177, 396, 672, 764
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 2 cut(s) 944, 1032
BmuI ACTGGG 1 cut(s) 520
Bpu1102I GCTNAGC 1 cut(s) 1132
BpuEI CTTGAG 1 cut(s) 677
BpuMI CCSGG 3 cut(s) 177, 396, 672
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 468, 768
BsaWI WCCGGW 1 cut(s) 591
BsaXI ACNNNNNCTCC 2 cut(s) 1244, 1274
Bsc4I CCNNNNNNNGG 3 cut(s) 395, 769, 810
Bse1I ACTGG 2 cut(s) 279, 526
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 1 cut(s) 764
BseDI CCNNGG 2 cut(s) 468, 768
BseGI GGATG 5 cut(s) 623, 804, 988, 1009, 1074
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 395, 769, 810
BseMII CTCAG 2 cut(s) 702, 1146
BseNI ACTGG 2 cut(s) 279, 526
BseRI GAGGAG 2 cut(s) 851, 1110
BseXI GCAGC 1 cut(s) 103
BsgI GTGCAG 2 cut(s) 129, 179
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 767
BshNI GGYRCC 1 cut(s) 986
BsiHKAI GWGCWC 2 cut(s) 323, 879
BsiSI CCGG 4 cut(s) 177, 395, 592, 671
BslFI GGGAC 2 cut(s) 840, 1182
BslI CCNNNNNNNGG 3 cut(s) 395, 769, 810
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 840, 1182
BsnI GGCC 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 323, 879
Bsp143I GATC 3 cut(s) 85, 1020, 1296
Bsp1720I GCTNAGC 1 cut(s) 1132
BspACI CCGC 1 cut(s) 510
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 2 cut(s) 703, 1145
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 3 cut(s) 988, 1170, 1171
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 2 cut(s) 80, 1291
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 986
BsrI ACTGG 2 cut(s) 279, 526
BssECI CCNNGG 2 cut(s) 468, 768
BssMI GATC 3 cut(s) 85, 1020, 1296
BssSI CACGAG 2 cut(s) 241, 448
BssT1I CCWWGG 2 cut(s) 468, 768
Bst2BI CACGAG 2 cut(s) 241, 448
Bst2UI CCWGG 1 cut(s) 764
Bst4CI ACNGT 4 cut(s) 200, 374, 920, 1156
Bst6I CTCTTC 2 cut(s) 263, 828
BstAPI GCANNNNNTGC 2 cut(s) 193, 796
BstC8I GCNNGC 5 cut(s) 6, 307, 311, 801, 1217
BstDEI CTNAG 3 cut(s) 711, 859, 1132
BstENI CCTNNNNNAGG 1 cut(s) 767
BstF5I GGATG 5 cut(s) 623, 804, 988, 1009, 1074
BstFNI CGCG 1 cut(s) 666
BstH2I RGCGCY 2 cut(s) 117, 763
BstHHI GCGC 4 cut(s) 116, 394, 666, 762
BstKTI GATC 3 cut(s) 88, 1023, 1299
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 3 cut(s) 85, 1020, 1296
BstMWI GCNNNNNNNGC 4 cut(s) 193, 796, 820, 1290
BstNI CCWGG 1 cut(s) 764
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 175, 394, 670, 762
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 1 cut(s) 103
BstX2I RGATCY 2 cut(s) 1020, 1296
BstXI CCANNNNNNTGG 2 cut(s) 978, 1000
BstYI RGATCY 2 cut(s) 1020, 1296
BsuRI GGCC 1 cut(s) 767
BtsCI GGATG 5 cut(s) 623, 804, 988, 1009, 1074
BtsIMutI CAGTG 2 cut(s) 379, 1238
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 5 cut(s) 6, 307, 311, 801, 1217
CaiI CAGNNNCTG 1 cut(s) 193
CfoI GCGC 4 cut(s) 116, 394, 666, 762
Cfr13I GGNCC 1 cut(s) 1169
CseI GACGC 1 cut(s) 1299
Csp6I GTAC 1 cut(s) 987
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviAII CATG 4 cut(s) 800, 914, 929, 1305
CviQI GTAC 1 cut(s) 987
DdeI CTNAG 3 cut(s) 711, 859, 1132
DpnI GATC 3 cut(s) 87, 1022, 1298
DpnII GATC 3 cut(s) 85, 1020, 1296
DrdI GACNNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 765
Eam1104I CTCTTC 2 cut(s) 263, 828
EarI CTCTTC 2 cut(s) 263, 828
Ecl136II GAGCTC 2 cut(s) 321, 877
Eco130I CCWWGG 2 cut(s) 468, 768
Eco24I GRGCYC 2 cut(s) 323, 879
Eco47I GGWCC 1 cut(s) 1169
Eco53kI GAGCTC 2 cut(s) 321, 877
Eco57I CTGAAG 3 cut(s) 402, 809, 836
EcoICRI GAGCTC 2 cut(s) 321, 877
EcoNI CCTNNNNNAGG 1 cut(s) 767
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 762
EcoT14I CCWWGG 2 cut(s) 468, 768
EcoT38I GRGCYC 2 cut(s) 323, 879
ErhI CCWWGG 2 cut(s) 468, 768
FaeI CATG 4 cut(s) 803, 917, 932, 1308
FalI AAGNNNNNCTT 2 cut(s) 670, 702
FaqI GGGAC 2 cut(s) 840, 1182
FatI CATG 4 cut(s) 799, 913, 928, 1304
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 5 cut(s) 630, 791, 995, 1016, 1081
FriOI GRGCYC 2 cut(s) 323, 879
Fsp4HI GCNGC 1 cut(s) 117
GlaI GCGC 4 cut(s) 115, 393, 665, 761
GluI GCNGC 1 cut(s) 117
HaeII RGCGCY 2 cut(s) 117, 763
HaeIII GGCC 1 cut(s) 767
HapII CCGG 4 cut(s) 177, 395, 592, 671
HgaI GACGC 1 cut(s) 1299
HhaI GCGC 4 cut(s) 116, 394, 666, 762
Hin1II CATG 4 cut(s) 803, 917, 932, 1308
Hin6I GCGC 4 cut(s) 114, 392, 664, 760
HinP1I GCGC 4 cut(s) 114, 392, 664, 760
HindIII AAGCTT 3 cut(s) 303, 311, 845
HinfI GANTC 6 cut(s) 98, 190, 437, 520, 698, 866
HpaII CCGG 4 cut(s) 177, 395, 592, 671
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 748, 1012
Hpy188I TCNGA 3 cut(s) 90, 421, 703
Hpy188III TCNNGA 6 cut(s) 135, 324, 827, 896, 1052, 1063
Hpy8I GTNNAC 2 cut(s) 748, 1012
Hpy99I CGWCG 4 cut(s) 504, 1128, 1131, 1293
HpyAV CCTTC 3 cut(s) 69, 811, 1024
HpyCH4III ACNGT 4 cut(s) 200, 374, 920, 1156
HpyCH4IV ACGT 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 796, 820, 1290
HpyF3I CTNAG 3 cut(s) 711, 859, 1132
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 4 cut(s) 803, 917, 932, 1308
HspAI GCGC 4 cut(s) 114, 392, 664, 760
KflI GGGWCCC 1 cut(s) 1169
KpnI GGTACC 1 cut(s) 990
Kzo9I GATC 3 cut(s) 85, 1020, 1296
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 5 cut(s) 179, 326, 472, 820, 882
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 2 cut(s) 944, 1032
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 28, 368
MalI GATC 3 cut(s) 87, 1022, 1298
MboI GATC 3 cut(s) 85, 1020, 1296
MboII GAAGA 5 cut(s) 107, 280, 845, 853, 1046
MflI RGATCY 2 cut(s) 1020, 1296
MhlI GDGCHC 2 cut(s) 323, 879
MlsI TGGCCA 1 cut(s) 767
MluCI AATT 9 cut(s) 102, 162, 424, 575, 899, 907, 923, 1055, 1147
MluNI TGGCCA 1 cut(s) 767
MlyI GAGTC 2 cut(s) 514, 692
MmeI TCCRAC 2 cut(s) 73, 1181
Mox20I TGGCCA 1 cut(s) 767
MscI TGGCCA 1 cut(s) 767
MslI CAYNNNNRTG 2 cut(s) 480, 918
Msp20I TGGCCA 1 cut(s) 767
MspI CCGG 4 cut(s) 177, 395, 592, 671
MspR9I CCNGG 4 cut(s) 177, 396, 672, 764
MvaI CCWGG 1 cut(s) 764
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 4 cut(s) 193, 796, 820, 1290
NciI CCSGG 3 cut(s) 177, 396, 672
NdeII GATC 3 cut(s) 85, 1020, 1296
NlaIII CATG 4 cut(s) 803, 917, 932, 1308
NlaIV GGNNCC 3 cut(s) 988, 1170, 1171
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 1 cut(s) 803
PaeI GCATGC 1 cut(s) 803
PciSI GCTCTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 98, 190, 437, 866
PflMI CCANNNNNTGG 1 cut(s) 810
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 514, 692
PpsI GAGTC 2 cut(s) 514, 692
PpuMI RGGWCCY 1 cut(s) 1169
Psp124BI GAGCTC 2 cut(s) 323, 879
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 762
PspGI CCWGG 1 cut(s) 762
PspN4I GGNNCC 3 cut(s) 988, 1170, 1171
PspPI GGNCC 1 cut(s) 1169
PspPPI RGGWCCY 1 cut(s) 1169
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 193
PsuI RGATCY 2 cut(s) 1020, 1296
RsaI GTAC 1 cut(s) 988
RsaNI GTAC 1 cut(s) 987
RseI CAYNNNNRTG 2 cut(s) 480, 918
SacI GAGCTC 2 cut(s) 323, 879
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 3 cut(s) 85, 1020, 1296
Sau96I GGNCC 1 cut(s) 1169
SchI GAGTC 2 cut(s) 514, 692
ScrFI CCNGG 4 cut(s) 177, 396, 672, 764
SduI GDGCHC 2 cut(s) 323, 879
SfaNI GCATC 2 cut(s) 944, 1032
SfcI CTRYAG 1 cut(s) 531
SinI GGWCC 1 cut(s) 1169
SmiMI CAYNNNNRTG 2 cut(s) 480, 918
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
SphI GCATGC 1 cut(s) 803
Sse9I AATT 9 cut(s) 102, 162, 424, 575, 899, 907, 923, 1055, 1147
SsiI CCGC 1 cut(s) 510
SstI GAGCTC 2 cut(s) 323, 879
StyD4I CCNGG 4 cut(s) 175, 394, 670, 762
StyI CCWWGG 2 cut(s) 468, 768
TaaI ACNGT 4 cut(s) 200, 374, 920, 1156
TaiI ACGT 1 cut(s) 446
TaqI TCGA 4 cut(s) 78, 84, 1229, 1288
TasI AATT 9 cut(s) 102, 162, 424, 575, 899, 907, 923, 1055, 1147
TfiI GAWTC 4 cut(s) 98, 190, 437, 866
TscAI CASTG 2 cut(s) 379, 1245
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 5 cut(s) 668, 1020, 1085, 1100, 1260
TspRI CASTG 2 cut(s) 379, 1245
Van91I CCANNNNNTGG 1 cut(s) 810
VpaK11BI GGWCC 1 cut(s) 1169
XagI CCTNNNNNAGG 1 cut(s) 767
XapI RAATTY 4 cut(s) 899, 923, 1055, 1147
XceI RCATGY 1 cut(s) 803
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.