MD11G1250300.v1.1

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011235
Physical Location & Seq
Reverse (-)
36246409 .. 36249928
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1250300.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGCAGAATCTGCACCGCGTTATATCCCGTCTTTCCTCAACTTCAATTGGCACAAGCACAACAAAGTTTCTGAGAGAGAAAAGCAAACCGACTTTGGGAAGCAATGGTGTGCTTCCGTTGAAGAATGATATCGTATCTTGTCAGTTGATCACTTCCCGATTCCTTTCTTTCAGCTCTGGAGGTGACGGTGAAAATGATGGTAAAGATGAGTGGGATAAACCCCCGTCTGGGACATTTAGCAATAATGCATCAGATGATTTGGGGTGGGATAAACCTTCATCTGGAACATTTAGCAATAATGTATCAGATAATTTGGGGTGGGATACTGCATCAGCATGGTCGACTGGACTGACTAAGGAGCATTTTGATGGGGAGGTGGTAGGCCGACGGACAAGTGGGCTTGAGCCATCTCAATCTTCGGTGGTATCTGGTTTGCAAGAAATTGAGGATAGAATAAGGGAACTGGAAGAAGAGAACAAGAAAAGTAAAGTGTTTGTGGATGGGTGGGGAGAGAGATTGCGGGAGATCAGTGTGCTTCTGAAGCAAGTGCGTGAGCCTGGTGCGAGAGGGTCTTATCTCAAGGACTCGGAGAAGGCTGAGATGTATACGAAGCACAAGGAAAACCCTGAGGTTTATACTGTTGAGCGGCTGGCAAAGGATTATAGGATCATGAGGCAGAGAGTGCATGCTATTCTTTGGCTCAAAGAGCTTGAGGAGGAAGAGGAGAAAAAACTCGGTCGTCCTTTAGATGATTCTGTTGAGCTCCTTCTTGACACCTGCCCAGAATTTTTTAATTCTCATGACCGCGAATTCCACGTGGCATCCCTTCCCTACAAACCTGATTTCAAGGTTATGCCAGAGGGTTGGGATGGGACCACCAGAGATTTGGATGAAGTTCATTATGAAATCTCGAAGAAAGAAGATGAAATGCTCTATCAAGAGTTTGTCCAGAGAATGAACTTCAACAAAATGAAAATGAAAGGAGAGGTTTTCTGCCACAAGCATAGTCGTCGCCGGACTTCAAACGGGTGGAACTTCACCGTGGAAAAACTAGGACCAAGGGGAAAGCGTGGAGGTGGCGGCGGCTGGAAGTTTGTCAGCCAGCCAGATGGTTCAAGCCGACCGCTGAATGAAACGGAGAAAATGTACGTGAAGCGAGAAACACCCCGCCGCCGGCGTAAGATCCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

398

Amino Acids

45.49

Weight (kDa)

7.14

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bot1p PF12298 192 - 300 3.7e-07 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 785
AccBSI CCGCTC 1 cut(s) 646
AccI GTMKAC 2 cut(s) 341, 605
AccII CGCG 2 cut(s) 18, 807
AciI CCGC 9 cut(s) 16, 520, 646, 805, 1080, 1083, 1124, 1168, 1171
AclWI GGATC 2 cut(s) 674, 1177
AcsI RAATTY 2 cut(s) 785, 809
AcuI CTGAAG 1 cut(s) 560
AcvI CACGTG 1 cut(s) 817
AfaI GTAC 1 cut(s) 1148
AfiI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
AgsI TTSAA 6 cut(s) 45, 121, 847, 964, 1023, 1116
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 3 cut(s) 174, 709, 763
AluI AGCT 3 cut(s) 174, 709, 763
Alw21I GWGCWC 1 cut(s) 765
AlwI GGATC 2 cut(s) 674, 1177
AlwNI CAGNNNCTG 1 cut(s) 10
AoxI GGCC 1 cut(s) 382
ApoI RAATTY 2 cut(s) 785, 809
AspS9I GGNCC 2 cut(s) 873, 1055
AsuHPI GGTGA 3 cut(s) 194, 200, 1030
AvaII GGWCC 2 cut(s) 873, 1055
AxyI CCTNAGG 1 cut(s) 627
BanII GRGCYC 1 cut(s) 765
BbrPI CACGTG 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 765
BccI CCATC 6 cut(s) 191, 362, 415, 494, 863, 1103
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 1 cut(s) 558
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 147
BfaI CTAG 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 785
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 4 cut(s) 647, 1081, 1084, 1171
BlsI GCNGC 4 cut(s) 648, 1082, 1085, 1172
Bme1390I CCNGG 1 cut(s) 558
Bme18I GGWCC 2 cut(s) 873, 1055
BmgT120I GGNCC 2 cut(s) 873, 1055
BmiI GGNNCC 1 cut(s) 874
BmrFI CCNGG 1 cut(s) 558
BmsI GCATC 3 cut(s) 257, 338, 830
BpmI CTGGAG 1 cut(s) 198
BpuEI CTTGAG 3 cut(s) 422, 563, 731
BsaAI YACGTR 2 cut(s) 817, 1150
BsaJI CCNNGG 2 cut(s) 1041, 1058
BsaXI ACNNNNNCTCC 2 cut(s) 1130, 1160
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
Bse118I RCCGGY 1 cut(s) 1173
Bse1I ACTGG 2 cut(s) 349, 468
Bse21I CCTNAGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 2 cut(s) 1041, 1058
BseGI GGATG 4 cut(s) 505, 821, 874, 895
BseLI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 3 cut(s) 62, 588, 618
BseNI ACTGG 2 cut(s) 349, 468
BseRI GAGGAG 2 cut(s) 728, 737
Bsh1236I CGCG 2 cut(s) 18, 807
Bsh1285I CGRYCG 2 cut(s) 739, 1124
BshFI GGCC 1 cut(s) 384
BsiEI CGRYCG 2 cut(s) 739, 1124
BsiHKAI GWGCWC 1 cut(s) 765
BsiSI CCGG 2 cut(s) 1015, 1174
BslFI GGGAC 2 cut(s) 244, 886
BslI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
BsmFI GGGAC 2 cut(s) 244, 886
BsnI GGCC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 4 cut(s) 147, 525, 666, 1182
BspACI CCGC 9 cut(s) 16, 520, 646, 805, 1080, 1083, 1124, 1168, 1171
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 3 cut(s) 63, 589, 619
BspFNI CGCG 2 cut(s) 18, 807
BspHI TCATGA 2 cut(s) 669, 799
BspLI GGNNCC 1 cut(s) 874
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 2 cut(s) 674, 1177
BsrBI CCGCTC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 1173
BsrI ACTGG 2 cut(s) 349, 468
BssAI RCCGGY 1 cut(s) 1173
BssECI CCNNGG 2 cut(s) 1041, 1058
BssMI GATC 4 cut(s) 147, 525, 666, 1182
BssNAI GTATAC 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 1058
Bst1107I GTATAC 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 188, 640, 1042
Bst6I CTCTTC 2 cut(s) 465, 714
BstAPI GCANNNNNTGC 2 cut(s) 10, 682
BstBAI YACGTR 2 cut(s) 817, 1150
BstC8I GCNNGC 4 cut(s) 651, 687, 1103, 1175
BstDEI CTNAG 4 cut(s) 71, 354, 597, 627
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 4 cut(s) 505, 821, 874, 895
BstFNI CGCG 2 cut(s) 18, 807
BstKTI GATC 4 cut(s) 150, 528, 669, 1185
BstMBI GATC 4 cut(s) 147, 525, 666, 1182
BstMCI CGRYCG 2 cut(s) 739, 1124
BstMWI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 706
BstNI CCWGG 1 cut(s) 558
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 556
BstUI CGCG 2 cut(s) 18, 807
BstX2I RGATCY 1 cut(s) 1182
BstXI CCANNNNNNTGG 3 cut(s) 864, 886, 1109
BstYI RGATCY 1 cut(s) 1182
BstZ17I GTATAC 1 cut(s) 606
Bsu36I CCTNAGG 1 cut(s) 627
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 4 cut(s) 505, 821, 874, 895
BtsIMutI CAGTG 1 cut(s) 535
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 4 cut(s) 651, 687, 1103, 1175
CaiI CAGNNNCTG 1 cut(s) 10
CciI TCATGA 2 cut(s) 669, 799
Cfr10I RCCGGY 1 cut(s) 1173
Cfr13I GGNCC 2 cut(s) 873, 1055
Csp6I GTAC 1 cut(s) 1147
CviAII CATG 4 cut(s) 336, 670, 686, 800
CviQI GTAC 1 cut(s) 1147
DdeI CTNAG 4 cut(s) 71, 354, 597, 627
DpnI GATC 4 cut(s) 149, 527, 668, 1184
DpnII GATC 4 cut(s) 147, 525, 666, 1182
Eam1104I CTCTTC 2 cut(s) 465, 714
EarI CTCTTC 2 cut(s) 465, 714
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 1058
Eco24I GRGCYC 1 cut(s) 765
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 873, 1055
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 1 cut(s) 560
Eco72I CACGTG 1 cut(s) 817
Eco81I CCTNAGG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 556
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 1058
EcoT22I ATGCAT 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 1058
FaeI CATG 4 cut(s) 339, 673, 689, 803
FaqI GGGAC 2 cut(s) 244, 886
FatI CATG 4 cut(s) 335, 669, 685, 799
FauI CCCGC 2 cut(s) 513, 1175
FbaI TGATCA 1 cut(s) 147
FblI GTMKAC 2 cut(s) 341, 605
Fnu4HI GCNGC 4 cut(s) 647, 1081, 1084, 1171
FokI GGATG 4 cut(s) 512, 808, 881, 902
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 4 cut(s) 647, 1081, 1084, 1171
FspBI CTAG 1 cut(s) 1052
GluI GCNGC 4 cut(s) 647, 1081, 1084, 1171
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 1 cut(s) 384
HapII CCGG 2 cut(s) 1015, 1174
Hin1II CATG 4 cut(s) 339, 673, 689, 803
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HinfI GANTC 4 cut(s) 7, 159, 584, 752
HpaII CCGG 2 cut(s) 1015, 1174
HphI GGTGA 3 cut(s) 194, 200, 1030
Hpy166II GTNNAC 2 cut(s) 342, 606
Hpy188I TCNGA 5 cut(s) 72, 253, 307, 540, 589
Hpy188III TCNNGA 9 cut(s) 156, 177, 282, 670, 770, 800, 910, 938, 949
Hpy8I GTNNAC 2 cut(s) 342, 606
Hpy99I CGWCG 2 cut(s) 390, 1014
HpyAV CCTTC 4 cut(s) 285, 586, 776, 836
HpyCH4III ACNGT 3 cut(s) 188, 640, 1042
HpyCH4IV ACGT 2 cut(s) 816, 1149
HpyCH4V TGCA 6 cut(s) 4, 13, 248, 329, 436, 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 706
HpyF3I CTNAG 4 cut(s) 71, 354, 597, 627
HpySE526I ACGT 2 cut(s) 816, 1149
Hsp92II CATG 4 cut(s) 339, 673, 689, 803
KroI GCCGGC 1 cut(s) 1173
KroNI GCCGGC 1 cut(s) 1175
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 4 cut(s) 147, 525, 666, 1182
LmnI GCTCC 2 cut(s) 358, 768
LweI GCATC 3 cut(s) 257, 338, 830
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 2 cut(s) 816, 1149
MaeIII GTNAC 1 cut(s) 182
MalI GATC 4 cut(s) 149, 527, 668, 1184
MbiI CCGCTC 1 cut(s) 646
MboI GATC 4 cut(s) 147, 525, 666, 1182
MboII GAAGA 7 cut(s) 133, 408, 479, 482, 731, 925, 932
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 1 cut(s) 1182
MhlI GDGCHC 1 cut(s) 765
MluCI AATT 6 cut(s) 45, 310, 441, 785, 793, 809
MlyI GAGTC 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 250
MreI CGCCGGCG 1 cut(s) 1173
MroNI GCCGGC 1 cut(s) 1173
MseI TTAA 1 cut(s) 792
MslI CAYNNNNRTG 2 cut(s) 334, 366
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 2 cut(s) 1015, 1174
MspR9I CCNGG 1 cut(s) 558
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 558
MvnI CGCG 2 cut(s) 18, 807
MwoI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 706
NaeI GCCGGC 1 cut(s) 1175
NdeII GATC 4 cut(s) 147, 525, 666, 1182
NgoMIV GCCGGC 1 cut(s) 1173
NlaIII CATG 4 cut(s) 339, 673, 689, 803
NlaIV GGNNCC 1 cut(s) 874
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 2 cut(s) 669, 799
PaqCI CACCTGC 1 cut(s) 785
PdiI GCCGGC 1 cut(s) 1175
PfeI GAWTC 3 cut(s) 7, 159, 752
PkrI GCNGC 4 cut(s) 648, 1082, 1085, 1172
PleI GAGTC 1 cut(s) 578
PmaCI CACGTG 1 cut(s) 817
PmlI CACGTG 1 cut(s) 817
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 2 cut(s) 817, 1150
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 1 cut(s) 556
PspCI CACGTG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 874
PspPI GGNCC 2 cut(s) 873, 1055
PstNI CAGNNNCTG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 1182
RsaI GTAC 1 cut(s) 1148
RsaNI GTAC 1 cut(s) 1147
RseI CAYNNNNRTG 2 cut(s) 334, 366
SacI GAGCTC 1 cut(s) 765
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 792
SatI GCNGC 4 cut(s) 647, 1081, 1084, 1171
Sau3AI GATC 4 cut(s) 147, 525, 666, 1182
Sau96I GGNCC 2 cut(s) 873, 1055
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 765
SfaNI GCATC 3 cut(s) 257, 338, 830
SgrAI CRCCGGYG 1 cut(s) 1173
SinI GGWCC 2 cut(s) 873, 1055
SmiMI CAYNNNNRTG 2 cut(s) 334, 366
SmlI CTYRAG 3 cut(s) 401, 578, 710
SmoI CTYRAG 3 cut(s) 401, 578, 710
SphI GCATGC 1 cut(s) 689
Sse9I AATT 6 cut(s) 45, 310, 441, 785, 793, 809
SsiI CCGC 9 cut(s) 16, 520, 646, 805, 1080, 1083, 1124, 1168, 1171
SspMI CTAG 1 cut(s) 1052
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 1058
TaaI ACNGT 3 cut(s) 188, 640, 1042
TaiI ACGT 2 cut(s) 819, 1152
TaqI TCGA 2 cut(s) 341, 911
TaqII GACCGA 1 cut(s) 725
TasI AATT 6 cut(s) 45, 310, 441, 785, 793, 809
TauI GCSGC 4 cut(s) 649, 1083, 1086, 1173
TfiI GAWTC 3 cut(s) 7, 159, 752
Tru1I TTAA 1 cut(s) 792
Tru9I TTAA 1 cut(s) 792
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 9 cut(s) 267, 887, 906, 918, 939, 971, 986, 992, 1146
TspGWI ACGGA 4 cut(s) 105, 403, 1151, 1179
TspRI CASTG 1 cut(s) 535
VpaK11BI GGWCC 2 cut(s) 873, 1055
XapI RAATTY 2 cut(s) 785, 809
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 2 cut(s) 341, 605
XspI CTAG 1 cut(s) 1052
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.