MD03G1229800.v1.1

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011235
Physical Location & Seq
Reverse (-)
31517679 .. 31521108
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1229800.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGCAGAATCTGCACCGTGTTATTTCTCGTCTTTCCTCAACTTCACTTGGCACGAGTACAGCAAAGTTTCTGAGAGAGAAAAGCAAACTGACGTTGGGAAGCACTGGTGTGCTTCCGTTGAAGAATGGTATTGAATCTTGTCAGTTTATCACTCCCCGATTCTTTTCTTTCAGCTCTGGAGGTGATGGTGAAAATGTTGGTAAAGACGAGTGGGATAAATCCCCGTCTGGGACATTCAGCAATAATGCATCAGATGATTTGGGGTGGGATAAACCACCGCCTGGAACATTTAGCAATAATGCATCAGATGATTTGGGGTGGGATACTGCATCATCGTGGTCGACTGGATTGACCAAGGAGCATTTTGATGGGGAGGTTGTAGGCCGACGGACAAGTGGGCCCGACCCATCTCAATCATCAGTGGTATCTGGTTTGCAAGAAATTGAGGATAGGATAAGGGAACTGGAAGAAGAGAACAAGAAAAGCAAAAAGTTTGTGGATGGGTGGGGAGAAAGATTGCGGGAGATAAGTGTGCTTCTGAAGCAAGTGCGTGAGCCTGGTGCCAGAGGGTCTTATCTCAAGGACTCAGAGAAGGCTGAGATGTATAAGAAGCACAAGGAAAACCCTGAGGTTTATACTGTTGAGAGGCTGGCTAAGGATTACAGGATTATGAGGCAGAGAGTGCATGCTATTCTTTGGCTCAAAGAGATTGAGGAGGAAGAGGAGAAAAAGCTTGGTCGTCCTTTAGATGATTCTGTTGAGCTCCTGCTTGACACCTGCCCAGAATTTTTTAATTCTCATGACCGCGAATTCCATGTGGCATCCCTTCCCTACAAACCTGATTTCAAGGTTATGCCAGAGGGTTGGGACGGTACCACCAGAGATTTGGATGAAGTTCATTATGAAATCTCGAAGAAAGAAGATGAAATGCTCTATCAAGAGTTTGTCCAGAGAATGAACTTCAACAAAATGAAAATGAAAGGAGAGGTCTTCTGCCACAAGCATAGTCGTCGTCGAACTTCAGACGGGTGGAACTTCACCGTTGAAAAACTCGGACCCAGAGGAAAGCGTGGAGGTGGCGGTGGCTGGAAGTTTGTCAGCCAGCCAGATGGTTCAAGCCGACCGCTGAATGAAACGGAGAAAATGTACGTGAAGCGAGAAACACCCCGCCGCCGACGCAAGATCCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

398

Amino Acids

45.52

Weight (kDa)

7.7

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bot1p PF12298 192 - 300 1.3e-07 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 785
Acc65I GGTACC 1 cut(s) 872
AccB1I GGYRCC 2 cut(s) 560, 872
AccB7I CCANNNNNTGG 1 cut(s) 281
AccI GTMKAC 1 cut(s) 341
AccII CGCG 1 cut(s) 807
AciI CCGC 7 cut(s) 278, 520, 805, 1080, 1124, 1168, 1171
AclWI GGATC 1 cut(s) 1177
AcsI RAATTY 2 cut(s) 785, 809
AcuI CTGAAG 2 cut(s) 560, 1005
AfaI GTAC 3 cut(s) 58, 874, 1148
AfiI CCNNNNNNNGG 2 cut(s) 228, 281
AgsI TTSAA 6 cut(s) 121, 134, 847, 964, 1046, 1116
AjnI CCWGG 2 cut(s) 280, 556
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 3 cut(s) 174, 733, 763
AluI AGCT 3 cut(s) 174, 733, 763
Alw21I GWGCWC 1 cut(s) 765
AlwI GGATC 1 cut(s) 1177
AlwNI CAGNNNCTG 1 cut(s) 10
AoxI GGCC 2 cut(s) 382, 398
ApaI GGGCCC 1 cut(s) 402
ApoI RAATTY 2 cut(s) 785, 809
Asp718I GGTACC 1 cut(s) 872
AspS9I GGNCC 3 cut(s) 398, 399, 1055
AsuHPI GGTGA 3 cut(s) 194, 200, 1030
AvaII GGWCC 1 cut(s) 1055
AxyI CCTNAGG 1 cut(s) 627
BaeGI GKGCMC 1 cut(s) 402
BanI GGYRCC 2 cut(s) 560, 872
BanII GRGCYC 2 cut(s) 402, 765
BauI CACGAG 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 765
BccI CCATC 5 cut(s) 179, 362, 415, 494, 1103
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 2 cut(s) 282, 558
BciVI GTATCC 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 785
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 1 cut(s) 1171
BlsI GCNGC 1 cut(s) 1172
Bme1390I CCNGG 2 cut(s) 282, 558
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 3 cut(s) 398, 399, 1055
BmiI GGNNCC 4 cut(s) 400, 562, 874, 1057
BmrFI CCNGG 2 cut(s) 282, 558
BmsI GCATC 4 cut(s) 257, 311, 338, 830
BpiI GAAGAC 1 cut(s) 982
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 1150
BsaJI CCNNGG 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 1130, 1160
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 281
Bse1I ACTGG 3 cut(s) 109, 349, 468
Bse21I CCTNAGG 1 cut(s) 627
BseBI CCWGG 2 cut(s) 282, 558
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 3 cut(s) 505, 821, 895
BseLI CCNNNNNNNGG 2 cut(s) 228, 281
BseMII CTCAG 4 cut(s) 62, 588, 600, 618
BseNI ACTGG 3 cut(s) 109, 349, 468
BseRI GAGGAG 2 cut(s) 728, 737
BseSI GKGCMC 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 807
Bsh1285I CGRYCG 1 cut(s) 1124
BshFI GGCC 2 cut(s) 384, 400
BshNI GGYRCC 2 cut(s) 560, 872
BsiEI CGRYCG 1 cut(s) 1124
BsiHKAI GWGCWC 1 cut(s) 765
BslFI GGGAC 2 cut(s) 244, 881
BslI CCNNNNNNNGG 2 cut(s) 228, 281
BsmFI GGGAC 2 cut(s) 244, 881
BsnI GGCC 2 cut(s) 384, 400
Bsp120I GGGCCC 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 402, 765
Bsp143I GATC 1 cut(s) 1182
BspACI CCGC 7 cut(s) 278, 520, 805, 1080, 1124, 1168, 1171
BspANI GGCC 2 cut(s) 384, 400
BspCNI CTCAG 4 cut(s) 63, 589, 599, 619
BspFNI CGCG 1 cut(s) 807
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 4 cut(s) 400, 562, 874, 1057
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 1 cut(s) 1177
BspT107I GGYRCC 2 cut(s) 560, 872
BsrI ACTGG 3 cut(s) 109, 349, 468
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 1 cut(s) 1182
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 354
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 2 cut(s) 282, 558
Bst4CI ACNGT 4 cut(s) 17, 640, 872, 1042
Bst6I CTCTTC 2 cut(s) 465, 714
BstAPI GCANNNNNTGC 2 cut(s) 10, 682
BstBAI YACGTR 1 cut(s) 1150
BstC8I GCNNGC 3 cut(s) 651, 687, 1103
BstDEI CTNAG 5 cut(s) 71, 586, 597, 627, 654
BstF5I GGATG 3 cut(s) 505, 821, 895
BstFNI CGCG 1 cut(s) 807
BstKTI GATC 1 cut(s) 1185
BstMBI GATC 1 cut(s) 1182
BstMCI CGRYCG 1 cut(s) 1124
BstMWI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 1176
BstNI CCWGG 2 cut(s) 282, 558
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 2 cut(s) 280, 556
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 807
BstV2I GAAGAC 1 cut(s) 982
BstX2I RGATCY 1 cut(s) 1182
BstXI CCANNNNNNTGG 3 cut(s) 864, 886, 1109
BstYI RGATCY 1 cut(s) 1182
Bsu36I CCTNAGG 1 cut(s) 627
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 2 cut(s) 384, 400
BtsCI GGATG 3 cut(s) 505, 821, 895
BtsIMutI CAGTG 2 cut(s) 102, 426
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 3 cut(s) 651, 687, 1103
CaiI CAGNNNCTG 1 cut(s) 10
CciI TCATGA 1 cut(s) 799
Cfr13I GGNCC 3 cut(s) 398, 399, 1055
CseI GACGC 1 cut(s) 1185
Csp6I GTAC 3 cut(s) 57, 873, 1147
CviAII CATG 3 cut(s) 686, 800, 815
CviQI GTAC 3 cut(s) 57, 873, 1147
DdeI CTNAG 5 cut(s) 71, 586, 597, 627, 654
DpnI GATC 1 cut(s) 1184
DpnII GATC 1 cut(s) 1182
Eam1104I CTCTTC 2 cut(s) 465, 714
EarI CTCTTC 2 cut(s) 465, 714
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 2 cut(s) 402, 765
Eco47I GGWCC 1 cut(s) 1055
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 560, 1005
Eco81I CCTNAGG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 2 cut(s) 280, 556
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 2 cut(s) 250, 304
EcoT38I GRGCYC 2 cut(s) 402, 765
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 3 cut(s) 689, 803, 818
FaiI YATR 9 cut(s) 606, 636, 671, 687, 801, 816, 854, 903, 1005
FaqI GGGAC 2 cut(s) 244, 881
FatI CATG 3 cut(s) 685, 799, 814
FauI CCCGC 2 cut(s) 513, 1175
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 1 cut(s) 1171
FokI GGATG 3 cut(s) 512, 808, 902
FriOI GRGCYC 2 cut(s) 402, 765
Fsp4HI GCNGC 1 cut(s) 1171
GluI GCNGC 1 cut(s) 1171
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 2 cut(s) 384, 400
HgaI GACGC 1 cut(s) 1185
Hin1II CATG 3 cut(s) 689, 803, 818
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 5 cut(s) 7, 134, 159, 584, 752
HphI GGTGA 3 cut(s) 194, 200, 1030
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 7 cut(s) 72, 253, 307, 540, 589, 1024, 1055
Hpy188III TCNNGA 5 cut(s) 177, 800, 910, 938, 949
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 4 cut(s) 390, 1014, 1017, 1179
HpyAV CCTTC 2 cut(s) 586, 836
HpyCH4III ACNGT 4 cut(s) 17, 640, 872, 1042
HpyCH4IV ACGT 2 cut(s) 92, 1149
HpyCH4V TGCA 7 cut(s) 4, 13, 248, 302, 329, 436, 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 1176
HpyF3I CTNAG 5 cut(s) 71, 586, 597, 627, 654
HpySE526I ACGT 2 cut(s) 92, 1149
Hsp92II CATG 3 cut(s) 689, 803, 818
KpnI GGTACC 1 cut(s) 876
Kzo9I GATC 1 cut(s) 1182
LmnI GCTCC 2 cut(s) 358, 768
LweI GCATC 4 cut(s) 257, 311, 338, 830
MaeII ACGT 2 cut(s) 92, 1149
MalI GATC 1 cut(s) 1184
MboI GATC 1 cut(s) 1182
MboII GAAGA 7 cut(s) 133, 479, 482, 731, 925, 932, 982
MflI RGATCY 1 cut(s) 1182
MhlI GDGCHC 2 cut(s) 402, 765
MluCI AATT 4 cut(s) 441, 785, 793, 809
MlyI GAGTC 1 cut(s) 578
Mph1103I ATGCAT 2 cut(s) 250, 304
MseI TTAA 1 cut(s) 792
MslI CAYNNNNRTG 3 cut(s) 107, 334, 366
MspA1I CMGCKG 1 cut(s) 1126
MspR9I CCNGG 2 cut(s) 282, 558
MvaI CCWGG 2 cut(s) 282, 558
MvnI CGCG 1 cut(s) 807
MwoI GCNNNNNNNGC 4 cut(s) 10, 541, 682, 1176
NdeII GATC 1 cut(s) 1182
NlaIII CATG 3 cut(s) 689, 803, 818
NlaIV GGNNCC 4 cut(s) 400, 562, 874, 1057
NsiI ATGCAT 2 cut(s) 250, 304
NspI RCATGY 1 cut(s) 689
OliI CACNNNNGTG 1 cut(s) 107
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 1 cut(s) 799
PaqCI CACCTGC 1 cut(s) 785
PfeI GAWTC 4 cut(s) 7, 134, 159, 752
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 1 cut(s) 1172
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 1150
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 2 cut(s) 280, 556
PspGI CCWGG 2 cut(s) 280, 556
PspN4I GGNNCC 4 cut(s) 400, 562, 874, 1057
PspOMI GGGCCC 1 cut(s) 398
PspPI GGNCC 3 cut(s) 398, 399, 1055
PstNI CAGNNNCTG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 1182
RsaI GTAC 3 cut(s) 58, 874, 1148
RsaNI GTAC 3 cut(s) 57, 873, 1147
RseI CAYNNNNRTG 3 cut(s) 107, 334, 366
SacI GAGCTC 1 cut(s) 765
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 792
SatI GCNGC 1 cut(s) 1171
Sau3AI GATC 1 cut(s) 1182
Sau96I GGNCC 3 cut(s) 398, 399, 1055
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 2 cut(s) 282, 558
SduI GDGCHC 2 cut(s) 402, 765
SfaNI GCATC 4 cut(s) 257, 311, 338, 830
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 3 cut(s) 107, 334, 366
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SphI GCATGC 1 cut(s) 689
Sse9I AATT 4 cut(s) 441, 785, 793, 809
SsiI CCGC 7 cut(s) 278, 520, 805, 1080, 1124, 1168, 1171
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 280, 556
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 4 cut(s) 17, 640, 872, 1042
TaiI ACGT 2 cut(s) 95, 1152
TaqI TCGA 3 cut(s) 341, 911, 1015
TasI AATT 4 cut(s) 441, 785, 793, 809
TatI WGTACW 1 cut(s) 56
TauI GCSGC 1 cut(s) 1173
TfiI GAWTC 4 cut(s) 7, 134, 159, 752
Tru1I TTAA 1 cut(s) 792
Tru9I TTAA 1 cut(s) 792
TscAI CASTG 2 cut(s) 109, 426
TspDTI ATGAA 8 cut(s) 887, 906, 918, 939, 971, 986, 992, 1146
TspGWI ACGGA 4 cut(s) 105, 403, 1151, 1179
TspRI CASTG 2 cut(s) 109, 426
Van91I CCANNNNNTGG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 2 cut(s) 785, 809
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 341
Zsp2I ATGCAT 2 cut(s) 250, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.