pycom11g22150

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011235
Physical Location & Seq
Reverse (-)
25149927 .. 25152017
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22150.1

Sequence Viewer

Length: 1194 bp
ATGCAGAATATGCACCGCTTTATATCCCGTCTTTCCTCAACTTCAATTGGCACAAGCACAACAAAGTTTCTGAGAGAGAAAAGCAAACCGACTTTGGGAAGCAATGGTGTGCTTCCATTGAAGAATGATATCGTATCTTGTCAGTTTATCACTTCCCGATTCTTTTCTTTCAGCTCTGGAGGTGACGGTGAAAATGATGGTAAAGATGAGTGGGATAAACCCCCGTCTGGGACATTTAGCAATAATGCATCAGATGATTTGGGGTGGGATAAACCTTCATCTGGAACATTCACCAATAATGCATCAGATAATTTGGGGTGGGATACTGCATCAGCATGGTCGACTGGACTGACCAAGGAGCATTTTGATGGGGAGGTGGTAGGCCGACGTACAAGTGGGCTCAACCCATCTCAGTCTTCAGTGGTATCTGGTTTGCAAGAAATTGAGGATAGAATAAGGGAACTGGAAGAAGAGAACAGGAAAAGTAAAACGTTTGTGGATGGGTGGGGAGAGAGATTGCAGGAGATAAGTGTGCTTCTGAAGCAAGTGCGCGAGCCAGGTGCGAGAGGATCTTATCTCAAGGACTCGGAGAAGGCTGAGATGTATAAGAAGCACAAGGAAAACCCTGAGGTTTATACTGTTGAGCGGCTGGCTAAGGATTATAGGATTATGAGGCAGAGAGTGCATGCTATTCTTTGGCTCAAAGAGCTTGAGGAGGAAGAGGAGAAAAAACTTGGTCGTCCTTTAGATGATTCTGTTGAGCTTCTTCTTGACACCTGCCCAGAATTTTTTAATTCTCATGACCGCGAATTCCATGTGGCATCCCTTCCCTACAAACCTGATTTCAAGGTTATGCCAGAGGGTTGGGATGGTACCACCAGAGATTTGGATGAAGTTCATTATGAAATCTCGAAGAAAGAAGATGAAATGCTCTATCAAGAGTTTGTCCAGAGAATGAACTTCAACAAAATGAAGATAAAAGGAGAGGTTTTCTGCCACAAGCATAGTCGTCGCCGGACTTCAAACGGGTGGAACTTCACCGTGGAAAAACTAGGACCAAGAGGAAAGCGTGGAGGTGGCGGCGGCTGGAAGTTTGTCAGCCAGCCAGATGGTTCAAGCCGACCGCTGAATGAAACGGAGAAAATGTACGTGAAGCGAGAAACACCCCGCCGCCGGAGCTCGATCCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

398

Amino Acids

45.5

Weight (kDa)

6.79

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bot1p PF12298 192 - 300 1.6e-07 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 785
Acc65I GGTACC 1 cut(s) 872
AccB1I GGYRCC 1 cut(s) 872
AccBSI CCGCTC 1 cut(s) 646
AccI GTMKAC 1 cut(s) 341
AccII CGCG 2 cut(s) 552, 807
AciI CCGC 8 cut(s) 16, 646, 805, 1080, 1083, 1124, 1168, 1171
AclI AACGTT 1 cut(s) 491
AclWI GGATC 2 cut(s) 577, 1177
AcsI RAATTY 2 cut(s) 785, 809
AcuI CTGAAG 2 cut(s) 402, 560
AfaI GTAC 3 cut(s) 391, 874, 1148
AfiI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
AgsI TTSAA 6 cut(s) 45, 121, 847, 964, 1023, 1116
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 4 cut(s) 174, 709, 763, 1179
AluI AGCT 4 cut(s) 174, 709, 763, 1179
Alw21I GWGCWC 1 cut(s) 1181
AlwI GGATC 2 cut(s) 577, 1177
AoxI GGCC 1 cut(s) 382
ApoI RAATTY 2 cut(s) 785, 809
Asp718I GGTACC 1 cut(s) 872
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 1 cut(s) 1055
AsuHPI GGTGA 4 cut(s) 194, 200, 283, 1030
AvaII GGWCC 1 cut(s) 1055
AxyI CCTNAGG 1 cut(s) 627
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 2 cut(s) 402, 1181
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 1181
BccI CCATC 6 cut(s) 191, 362, 415, 494, 863, 1103
BcgI CGANNNNNNTGC 4 cut(s) 542, 576, 992, 1026
BciT130I CCWGG 1 cut(s) 558
BciVI GTATCC 1 cut(s) 316
BfaI CTAG 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 785
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 4 cut(s) 647, 1081, 1084, 1171
BlsI GCNGC 4 cut(s) 648, 1082, 1085, 1172
Bme1390I CCNGG 1 cut(s) 558
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 1 cut(s) 1055
BmiI GGNNCC 1 cut(s) 874
BmrFI CCNGG 1 cut(s) 558
BmsI GCATC 4 cut(s) 257, 311, 338, 830
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 2 cut(s) 563, 731
BsaAI YACGTR 1 cut(s) 1150
BsaJI CCNNGG 2 cut(s) 354, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 1130, 1160
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
Bse1I ACTGG 2 cut(s) 349, 468
Bse21I CCTNAGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 2 cut(s) 354, 1041
BseGI GGATG 4 cut(s) 505, 821, 874, 895
BseLI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 4 cut(s) 62, 425, 588, 618
BseNI ACTGG 2 cut(s) 349, 468
BseRI GAGGAG 2 cut(s) 728, 737
Bsh1236I CGCG 2 cut(s) 552, 807
Bsh1285I CGRYCG 1 cut(s) 1124
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 872
BsiEI CGRYCG 1 cut(s) 1124
BsiHKAI GWGCWC 1 cut(s) 1181
BsiSI CCGG 2 cut(s) 1015, 1174
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 5 cut(s) 95, 227, 228, 281, 1173
BsmFI GGGAC 1 cut(s) 244
BsnI GGCC 1 cut(s) 384
Bsp1286I GDGCHC 2 cut(s) 402, 1181
Bsp143I GATC 2 cut(s) 569, 1182
BspACI CCGC 8 cut(s) 16, 646, 805, 1080, 1083, 1124, 1168, 1171
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 4 cut(s) 63, 424, 589, 619
BspFNI CGCG 2 cut(s) 552, 807
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 1 cut(s) 874
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 2 cut(s) 577, 1177
BspT107I GGYRCC 1 cut(s) 872
BsrBI CCGCTC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 2 cut(s) 349, 468
BssECI CCNNGG 2 cut(s) 354, 1041
BssMI GATC 2 cut(s) 569, 1182
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 188, 640, 1042
Bst6I CTCTTC 2 cut(s) 465, 714
BstAPI GCANNNNNTGC 2 cut(s) 10, 682
BstBAI YACGTR 1 cut(s) 1150
BstC8I GCNNGC 4 cut(s) 554, 651, 687, 1103
BstDEI CTNAG 5 cut(s) 71, 411, 597, 627, 654
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 4 cut(s) 505, 821, 874, 895
BstFNI CGCG 2 cut(s) 552, 807
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 2 cut(s) 572, 1185
BstMBI GATC 2 cut(s) 569, 1182
BstMCI CGRYCG 1 cut(s) 1124
BstMWI GCNNNNNNNGC 5 cut(s) 10, 541, 682, 706, 1176
BstNI CCWGG 1 cut(s) 558
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 556
BstUI CGCG 2 cut(s) 552, 807
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 569
BstXI CCANNNNNNTGG 3 cut(s) 864, 886, 1109
BstYI RGATCY 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 627
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 4 cut(s) 505, 821, 874, 895
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 4 cut(s) 554, 651, 687, 1103
CciI TCATGA 1 cut(s) 799
CfoI GCGC 1 cut(s) 552
Cfr13I GGNCC 1 cut(s) 1055
Csp6I GTAC 3 cut(s) 390, 873, 1147
CviAII CATG 4 cut(s) 336, 686, 800, 815
CviQI GTAC 3 cut(s) 390, 873, 1147
DdeI CTNAG 5 cut(s) 71, 411, 597, 627, 654
DpnI GATC 2 cut(s) 571, 1184
DpnII GATC 2 cut(s) 569, 1182
Eam1104I CTCTTC 2 cut(s) 465, 714
EarI CTCTTC 2 cut(s) 465, 714
Ecl136II GAGCTC 1 cut(s) 1179
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 2 cut(s) 402, 1181
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 1055
Eco53kI GAGCTC 1 cut(s) 1179
Eco57I CTGAAG 2 cut(s) 402, 560
Eco81I CCTNAGG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 1179
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 556
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 2 cut(s) 250, 304
EcoT38I GRGCYC 2 cut(s) 402, 1181
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 4 cut(s) 339, 689, 803, 818
FaqI GGGAC 1 cut(s) 244
FatI CATG 4 cut(s) 335, 685, 799, 814
FauI CCCGC 1 cut(s) 1175
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 4 cut(s) 647, 1081, 1084, 1171
FokI GGATG 4 cut(s) 512, 808, 881, 902
FriOI GRGCYC 2 cut(s) 402, 1181
Fsp4HI GCNGC 4 cut(s) 647, 1081, 1084, 1171
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 1 cut(s) 551
GluI GCNGC 4 cut(s) 647, 1081, 1084, 1171
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 1 cut(s) 384
HapII CCGG 2 cut(s) 1015, 1174
HhaI GCGC 1 cut(s) 552
Hin1II CATG 4 cut(s) 339, 689, 803, 818
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HinfI GANTC 3 cut(s) 159, 584, 752
HpaII CCGG 2 cut(s) 1015, 1174
HphI GGTGA 4 cut(s) 194, 200, 283, 1030
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 5 cut(s) 72, 253, 307, 540, 589
Hpy188III TCNNGA 8 cut(s) 156, 177, 282, 770, 800, 910, 938, 949
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 2 cut(s) 390, 1014
HpyAV CCTTC 3 cut(s) 285, 586, 836
HpyCH4III ACNGT 3 cut(s) 188, 640, 1042
HpyCH4IV ACGT 3 cut(s) 388, 491, 1149
HpyCH4V TGCA 8 cut(s) 4, 13, 248, 302, 329, 436, 520, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 541, 682, 706, 1176
HpyF3I CTNAG 5 cut(s) 71, 411, 597, 627, 654
HpySE526I ACGT 3 cut(s) 388, 491, 1149
Hsp92II CATG 4 cut(s) 339, 689, 803, 818
HspAI GCGC 1 cut(s) 550
KpnI GGTACC 1 cut(s) 876
Kzo9I GATC 2 cut(s) 569, 1182
LmnI GCTCC 2 cut(s) 358, 1176
LweI GCATC 4 cut(s) 257, 311, 338, 830
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 3 cut(s) 388, 491, 1149
MaeIII GTNAC 1 cut(s) 182
MalI GATC 2 cut(s) 571, 1184
MbiI CCGCTC 1 cut(s) 646
MboI GATC 2 cut(s) 569, 1182
MboII GAAGA 9 cut(s) 133, 408, 479, 482, 731, 758, 925, 932, 985
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 1 cut(s) 569
MhlI GDGCHC 2 cut(s) 402, 1181
MluCI AATT 6 cut(s) 45, 310, 441, 785, 793, 809
MlyI GAGTC 1 cut(s) 578
Mph1103I ATGCAT 2 cut(s) 250, 304
MseI TTAA 1 cut(s) 792
MslI CAYNNNNRTG 2 cut(s) 334, 366
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 2 cut(s) 1015, 1174
MspR9I CCNGG 1 cut(s) 558
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 558
MvnI CGCG 2 cut(s) 552, 807
MwoI GCNNNNNNNGC 5 cut(s) 10, 541, 682, 706, 1176
NdeII GATC 2 cut(s) 569, 1182
NlaIII CATG 4 cut(s) 339, 689, 803, 818
NlaIV GGNNCC 1 cut(s) 874
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 2 cut(s) 250, 304
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 1 cut(s) 799
PaqCI CACCTGC 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 1187
PfeI GAWTC 2 cut(s) 159, 752
PkrI GCNGC 4 cut(s) 648, 1082, 1085, 1172
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 1150
Psp124BI GAGCTC 1 cut(s) 1181
Psp1406I AACGTT 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 556
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 874
PspPI GGNCC 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 569
RsaI GTAC 3 cut(s) 391, 874, 1148
RsaNI GTAC 3 cut(s) 390, 873, 1147
RseI CAYNNNNRTG 2 cut(s) 334, 366
SacI GAGCTC 1 cut(s) 1181
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 792
SatI GCNGC 4 cut(s) 647, 1081, 1084, 1171
Sau3AI GATC 2 cut(s) 569, 1182
Sau96I GGNCC 1 cut(s) 1055
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 2 cut(s) 402, 1181
SfaNI GCATC 4 cut(s) 257, 311, 338, 830
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 2 cut(s) 334, 366
SmlI CTYRAG 2 cut(s) 578, 710
SmoI CTYRAG 2 cut(s) 578, 710
SphI GCATGC 1 cut(s) 689
Sse9I AATT 6 cut(s) 45, 310, 441, 785, 793, 809
SsiI CCGC 8 cut(s) 16, 646, 805, 1080, 1083, 1124, 1168, 1171
SspMI CTAG 1 cut(s) 1052
SstI GAGCTC 1 cut(s) 1181
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 188, 640, 1042
TaiI ACGT 3 cut(s) 391, 494, 1152
TaqI TCGA 3 cut(s) 341, 911, 1181
TasI AATT 6 cut(s) 45, 310, 441, 785, 793, 809
TauI GCSGC 4 cut(s) 649, 1083, 1086, 1173
TfiI GAWTC 2 cut(s) 159, 752
Tru1I TTAA 1 cut(s) 792
Tru9I TTAA 1 cut(s) 792
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 8 cut(s) 267, 887, 906, 918, 939, 971, 986, 1146
TspGWI ACGGA 2 cut(s) 1151, 1179
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 2 cut(s) 785, 809
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 341
XspI CTAG 1 cut(s) 1052
Zsp2I ATGCAT 2 cut(s) 250, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.