Rh5DG341800

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011235
Physical Location & Seq
Forward (+)
46577553 .. 46580886
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG341800.1

Sequence Viewer

Length: 1332 bp
ATGTGTATGCGGAAGAAAGGGAAAAGCCTGCGAGGGTTTTGGGGTTTAGATGTTACCATCTCTCAAATAATCCAACTCATTCCTTCGACTTACAAATGCTTCGATTTCGATCCGATTGAAGGTTCAATTTCTGTGGGCGCTGCTTCAATCGTCATTTCAGGATATTCTGCACCTGTGGGTTGGCAAATTAGATTACTTCCCGGAGCTATGCAGAATCTGCACCGTCTTATATCTCGTCTTTCCTCAACTTCAATCTGCAAAAGCACGACTACGACAAAGTTTCTGGGGGAGAAGAGCAACGGCAGTTTGGGAAACAATGGTGTTGAAGCTTGTAAGCCTATGAGCTCCCGATTTTTTTCTTTTCAATCTGGTGGTGATGGTCAAAATGATGGTAACAGTGGGTGGGATAAACCTGCGGCGGGAACATTTGGCAATAATGCATCAGATAATTTGGGGTGGGATTCTACGTCCTCGTGGTCGCTTGGACTGACCAAGGAGCATTTTGATGGGGAGGTGGTGGGGCGACGGACGAGCGGAGATGAGACTCCCCAGTCTGCAGTCATCTCTGGTTTGCAAGAGATTGAGGATAGAATAAAGGAATTGGAGGCAGAGAACCGGAAAAGTAAGAGATTTGTTGATGGATGGGGAGAGAGATTGAAAGAGATAAGTGTGCTGATGAAGCAAGTGCGCGAACCCGGTGCAAGAGGGTCTTATCTCAAGGACTCGGAAAAGGCTGAGATGTATCGATTGCACAAGGAAAACCCTGATGTTTACACTGTTGAGCGCCTGGCCAAGGATTATAGGATTATGAGGCAGAGAGTGCATGCCATTCTTTGGCTGAAGGAGCTTCAGGAGGAAGAGGAGAAGAAGCTTGGTCGTCCCTTAGATGATTCTGTTGAGCTCCTGCTTGACAGCTTTCCTGAATTTTTCAATTCCCATGACCGTGAATTCCATGTTGCATCACTTCCTTACAAACCTGACTTCAAGGTTATGCCAGAGGGTTGGGATGGTACCACCAGAGATTTGGATGAAGTCCACTATGAGATCTCCAAGAAGGAAGATGAGATGCTGTATCAAGAATTTGTCCAGAGGATGAACTTCAACAAAATGAAAATGAGAGGAGAGGTTTTCCACCACAAGTATAGTCGTCGTCGCTCTGCAGATGGGTGGAATTTCACGGTGGAGAAAATGGGACCCAAAGGAAAGCGTGGAGGTGGTGGTGGCTGGAAGTTTGTCAGCCTGCCAGATGGTTCGAGCCGACCACTGAATGAAAAGGAGAAAATGTATGTGAAACGAGAAACTCCACGCCATCGACGCAAGATCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

443

Amino Acids

50.43

Weight (kDa)

8.97

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-L20 PF12824 210 - 287 4e-06 Mitochondrial ribosomal protein subunit L20
Bot1p PF12298 238 - 342 8.4e-06 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 550
Acc36I ACCTGC 1 cut(s) 421
Acc65I GGTACC 1 cut(s) 1010
AccB1I GGYRCC 1 cut(s) 1010
AccB7I CCANNNNNTGG 1 cut(s) 834
AccBSI CCGCTC 1 cut(s) 534
AccII CGCG 1 cut(s) 690
AciI CCGC 4 cut(s) 10, 416, 419, 534
AclWI GGATC 2 cut(s) 104, 1315
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 4 cut(s) 923, 947, 1079, 1171
AcuI CTGAAG 2 cut(s) 833, 860
AfaI GTAC 1 cut(s) 1012
AfiI CCNNNNNNNGG 4 cut(s) 119, 419, 793, 834
AjnI CCWGG 1 cut(s) 786
AluBI AGCT 7 cut(s) 206, 329, 345, 847, 871, 901, 915
AluI AGCT 7 cut(s) 206, 329, 345, 847, 871, 901, 915
Alw21I GWGCWC 2 cut(s) 347, 903
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 2 cut(s) 104, 1315
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 1 cut(s) 789
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 4 cut(s) 923, 947, 1079, 1171
Asp718I GGTACC 1 cut(s) 1010
AspLEI GCGC 3 cut(s) 140, 690, 786
AspS9I GGNCC 1 cut(s) 1193
AsuC2I CCSGG 2 cut(s) 201, 696
AsuHPI GGTGA 1 cut(s) 386
AvaII GGWCC 1 cut(s) 1193
BalI TGGCCA 1 cut(s) 791
BanI GGYRCC 1 cut(s) 1010
BanII GRGCYC 2 cut(s) 347, 903
BauI CACGAG 1 cut(s) 472
Bbv12I GWGCWC 2 cut(s) 347, 903
BbvI GCAGC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 1 cut(s) 788
BcnI CCSGG 2 cut(s) 201, 696
BcoDI GTCTC 1 cut(s) 536
BfmI CTRYAG 2 cut(s) 555, 1158
BfoI RGCGCY 2 cut(s) 141, 787
BfuAI ACCTGC 1 cut(s) 421
BglII AGATCT 1 cut(s) 1044
BisI GCNGC 2 cut(s) 141, 417
BlsI GCNGC 2 cut(s) 142, 418
Bme1390I CCNGG 3 cut(s) 201, 696, 788
Bme18I GGWCC 1 cut(s) 1193
BmgT120I GGNCC 1 cut(s) 1193
BmiI GGNNCC 3 cut(s) 1012, 1194, 1195
BmrFI CCNGG 3 cut(s) 201, 696, 788
BmrI ACTGGG 1 cut(s) 544
BmsI GCATC 3 cut(s) 449, 968, 1056
BmuI ACTGGG 1 cut(s) 544
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 2 cut(s) 201, 696
Bsa29I ATCGAT 1 cut(s) 745
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 492, 792
BsaWI WCCGGW 1 cut(s) 615
BsaXI ACNNNNNCTCC 2 cut(s) 1268, 1298
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 419, 793, 834
Bse1I ACTGG 1 cut(s) 550
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 788
BseCI ATCGAT 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 492, 792
BseGI GGATG 4 cut(s) 647, 1012, 1033, 1098
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 4 cut(s) 119, 419, 793, 834
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 2 cut(s) 875, 1134
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 2 cut(s) 153, 203
Bsh1236I CGCG 1 cut(s) 690
BshFI GGCC 1 cut(s) 791
BshNI GGYRCC 1 cut(s) 1010
BshVI ATCGAT 1 cut(s) 745
BsiHKAI GWGCWC 2 cut(s) 347, 903
BsiSI CCGG 3 cut(s) 201, 616, 696
BslFI GGGAC 2 cut(s) 864, 1206
BslI CCNNNNNNNGG 4 cut(s) 119, 419, 793, 834
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 2 cut(s) 864, 1206
BsnI GGCC 1 cut(s) 791
Bsp1286I GDGCHC 2 cut(s) 347, 903
Bsp143I GATC 3 cut(s) 109, 1044, 1320
BspACI CCGC 4 cut(s) 10, 416, 419, 534
BspANI GGCC 1 cut(s) 791
BspCNI CTCAG 1 cut(s) 727
BspDI ATCGAT 1 cut(s) 745
BspFNI CGCG 1 cut(s) 690
BspLI GGNNCC 3 cut(s) 1012, 1194, 1195
BspMAI CTGCAG 2 cut(s) 559, 1162
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 2 cut(s) 104, 1315
BspQI GCTCTTC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 1010
BsrBI CCGCTC 1 cut(s) 534
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 492, 792
BssMI GATC 3 cut(s) 109, 1044, 1320
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 2 cut(s) 492, 792
Bst2BI CACGAG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 788
Bst4CI ACNGT 5 cut(s) 224, 398, 778, 944, 1180
Bst6I CTCTTC 2 cut(s) 287, 852
BstAPI GCANNNNNTGC 2 cut(s) 217, 820
BstC8I GCNNGC 3 cut(s) 29, 825, 1241
BstDEI CTNAG 2 cut(s) 735, 883
BstENI CCTNNNNNAGG 1 cut(s) 791
BstF5I GGATG 4 cut(s) 647, 1012, 1033, 1098
BstFNI CGCG 1 cut(s) 690
BstH2I RGCGCY 2 cut(s) 141, 787
BstHHI GCGC 3 cut(s) 140, 690, 786
BstKTI GATC 3 cut(s) 112, 1047, 1323
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 3 cut(s) 109, 1044, 1320
BstMWI GCNNNNNNNGC 5 cut(s) 217, 679, 820, 844, 1314
BstNI CCWGG 1 cut(s) 788
BstNSI RCATGY 1 cut(s) 827
BstSCI CCNGG 3 cut(s) 199, 694, 786
BstSFI CTRYAG 2 cut(s) 555, 1158
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 2 cut(s) 1044, 1320
BstXI CCANNNNNNTGG 2 cut(s) 1002, 1024
BstYI RGATCY 2 cut(s) 1044, 1320
Bsu15I ATCGAT 1 cut(s) 745
BsuRI GGCC 1 cut(s) 791
BsuTUI ATCGAT 1 cut(s) 745
BtsCI GGATG 4 cut(s) 647, 1012, 1033, 1098
BtsIMutI CAGTG 3 cut(s) 403, 774, 1262
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 3 cut(s) 29, 825, 1241
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 3 cut(s) 140, 690, 786
Cfr13I GGNCC 1 cut(s) 1193
ClaI ATCGAT 1 cut(s) 745
CseI GACGC 1 cut(s) 1323
Csp6I GTAC 1 cut(s) 1011
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 4 cut(s) 824, 938, 953, 1329
CviQI GTAC 1 cut(s) 1011
DdeI CTNAG 2 cut(s) 735, 883
DpnI GATC 3 cut(s) 111, 1046, 1322
DpnII GATC 3 cut(s) 109, 1044, 1320
DrdI GACNNNNNNGTC 1 cut(s) 550
DseDI GACNNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 2 cut(s) 287, 852
EarI CTCTTC 2 cut(s) 287, 852
Ecl136II GAGCTC 2 cut(s) 345, 901
Eco130I CCWWGG 2 cut(s) 492, 792
Eco24I GRGCYC 2 cut(s) 347, 903
Eco47I GGWCC 1 cut(s) 1193
Eco53kI GAGCTC 2 cut(s) 345, 901
Eco57I CTGAAG 2 cut(s) 833, 860
EcoICRI GAGCTC 2 cut(s) 345, 901
EcoNI CCTNNNNNAGG 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 1193
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 786
EcoT14I CCWWGG 2 cut(s) 492, 792
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 2 cut(s) 347, 903
ErhI CCWWGG 2 cut(s) 492, 792
FaeI CATG 4 cut(s) 827, 941, 956, 1332
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FaqI GGGAC 2 cut(s) 864, 1206
FatI CATG 4 cut(s) 823, 937, 952, 1328
FauI CCCGC 1 cut(s) 412
Fnu4HI GCNGC 2 cut(s) 141, 417
FokI GGATG 4 cut(s) 654, 1019, 1040, 1105
FriOI GRGCYC 2 cut(s) 347, 903
Fsp4HI GCNGC 2 cut(s) 141, 417
GlaI GCGC 3 cut(s) 139, 689, 785
GluI GCNGC 2 cut(s) 141, 417
HaeII RGCGCY 2 cut(s) 141, 787
HaeIII GGCC 1 cut(s) 791
HapII CCGG 3 cut(s) 201, 616, 696
HgaI GACGC 1 cut(s) 1323
HhaI GCGC 3 cut(s) 140, 690, 786
Hin1II CATG 4 cut(s) 827, 941, 956, 1332
Hin6I GCGC 3 cut(s) 138, 688, 784
HinP1I GCGC 3 cut(s) 138, 688, 784
HindIII AAGCTT 2 cut(s) 327, 869
HinfI GANTC 5 cut(s) 214, 461, 544, 722, 890
HpaII CCGG 3 cut(s) 201, 616, 696
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 772, 1036
Hpy188I TCNGA 3 cut(s) 114, 445, 727
Hpy188III TCNNGA 6 cut(s) 159, 348, 851, 920, 1076, 1087
Hpy8I GTNNAC 2 cut(s) 772, 1036
Hpy99I CGWCG 4 cut(s) 528, 1152, 1155, 1317
HpyAV CCTTC 4 cut(s) 93, 113, 835, 1048
HpyCH4III ACNGT 5 cut(s) 224, 398, 778, 944, 1180
HpyCH4IV ACGT 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 679, 820, 844, 1314
HpyF3I CTNAG 2 cut(s) 735, 883
HpySE526I ACGT 1 cut(s) 467
Hsp92II CATG 4 cut(s) 827, 941, 956, 1332
HspAI GCGC 3 cut(s) 138, 688, 784
KflI GGGWCCC 1 cut(s) 1193
KpnI GGTACC 1 cut(s) 1014
Kzo9I GATC 3 cut(s) 109, 1044, 1320
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 5 cut(s) 203, 350, 496, 844, 906
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 3 cut(s) 449, 968, 1056
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 2 cut(s) 52, 392
MalI GATC 3 cut(s) 111, 1046, 1322
MbiI CCGCTC 1 cut(s) 534
MboI GATC 3 cut(s) 109, 1044, 1320
MboII GAAGA 5 cut(s) 25, 304, 869, 877, 1070
MflI RGATCY 2 cut(s) 1044, 1320
MhlI GDGCHC 2 cut(s) 347, 903
MlsI TGGCCA 1 cut(s) 791
MluCI AATT 9 cut(s) 126, 186, 448, 599, 923, 931, 947, 1079, 1171
MluNI TGGCCA 1 cut(s) 791
MlyI GAGTC 2 cut(s) 538, 716
MmeI TCCRAC 1 cut(s) 97
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 1 cut(s) 442
MscI TGGCCA 1 cut(s) 791
MslI CAYNNNNRTG 2 cut(s) 504, 942
Msp20I TGGCCA 1 cut(s) 791
MspI CCGG 3 cut(s) 201, 616, 696
MspR9I CCNGG 3 cut(s) 201, 696, 788
MvaI CCWGG 1 cut(s) 788
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 5 cut(s) 217, 679, 820, 844, 1314
NciI CCSGG 2 cut(s) 201, 696
NdeII GATC 3 cut(s) 109, 1044, 1320
NlaIII CATG 4 cut(s) 827, 941, 956, 1332
NlaIV GGNNCC 3 cut(s) 1012, 1194, 1195
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 827
PaeI GCATGC 1 cut(s) 827
PciSI GCTCTTC 1 cut(s) 287
PfeI GAWTC 3 cut(s) 214, 461, 890
PflMI CCANNNNNTGG 1 cut(s) 834
PfoI TCCNGGA 1 cut(s) 199
PkrI GCNGC 2 cut(s) 142, 418
PleI GAGTC 2 cut(s) 538, 716
PpsI GAGTC 2 cut(s) 538, 716
PpuMI RGGWCCY 1 cut(s) 1193
Psp124BI GAGCTC 2 cut(s) 347, 903
Psp5II RGGWCCY 1 cut(s) 1193
Psp6I CCWGG 1 cut(s) 786
PspGI CCWGG 1 cut(s) 786
PspN4I GGNNCC 3 cut(s) 1012, 1194, 1195
PspPI GGNCC 1 cut(s) 1193
PspPPI RGGWCCY 1 cut(s) 1193
PstI CTGCAG 2 cut(s) 559, 1162
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 2 cut(s) 1044, 1320
RsaI GTAC 1 cut(s) 1012
RsaNI GTAC 1 cut(s) 1011
RseI CAYNNNNRTG 2 cut(s) 504, 942
SacI GAGCTC 2 cut(s) 347, 903
SapI GCTCTTC 1 cut(s) 287
SatI GCNGC 2 cut(s) 141, 417
Sau3AI GATC 3 cut(s) 109, 1044, 1320
Sau96I GGNCC 1 cut(s) 1193
SchI GAGTC 2 cut(s) 538, 716
ScrFI CCNGG 3 cut(s) 201, 696, 788
SduI GDGCHC 2 cut(s) 347, 903
SfaNI GCATC 3 cut(s) 449, 968, 1056
SfcI CTRYAG 2 cut(s) 555, 1158
SinI GGWCC 1 cut(s) 1193
SmiMI CAYNNNNRTG 2 cut(s) 504, 942
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SphI GCATGC 1 cut(s) 827
Sse9I AATT 9 cut(s) 126, 186, 448, 599, 923, 931, 947, 1079, 1171
SsiI CCGC 4 cut(s) 10, 416, 419, 534
SstI GAGCTC 2 cut(s) 347, 903
StyD4I CCNGG 3 cut(s) 199, 694, 786
StyI CCWWGG 2 cut(s) 492, 792
TaaI ACNGT 5 cut(s) 224, 398, 778, 944, 1180
TaiI ACGT 1 cut(s) 470
TaqI TCGA 6 cut(s) 86, 102, 108, 745, 1253, 1312
TasI AATT 9 cut(s) 126, 186, 448, 599, 923, 931, 947, 1079, 1171
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 3 cut(s) 214, 461, 890
TscAI CASTG 3 cut(s) 403, 781, 1269
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 5 cut(s) 692, 1044, 1109, 1124, 1284
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 3 cut(s) 403, 781, 1269
Van91I CCANNNNNTGG 1 cut(s) 834
VpaK11BI GGWCC 1 cut(s) 1193
XagI CCTNNNNNAGG 1 cut(s) 791
XapI RAATTY 4 cut(s) 923, 947, 1079, 1171
XceI RCATGY 1 cut(s) 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 1021
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.