Rroxscaffold_1G00033070

Eukaryotic mitochondrial regulator protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011235
Physical Location & Seq
Reverse (-)
47617999 .. 47621736
3738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033070.1

Sequence Viewer

Length: 1125 bp
ATGCAGAATCTGCACCGTCTTATATCTCATCTTTCCTCAACTTCAATCTGCAAAAGCACGACTACGACAAGGTTTCTGGGGGAGAAGAGCAACGCCAGTTTGGGAAACAATGGTGTTGAAGCTTGTAAGCTTATGAGCTCCCGATTTTTTTCTTTCCAATCTGGTGGTGATGGTCAAAATGATGGTAACAGTGGGTGGGATAAACCTGTGGCGGGAACATTTGGCAATAATGCATCAGATAATTTGGGGTGGGATTCTACGTCCTCGTGGTCGCTTGGACTGACCAAGGAGCATTTTGATGGGGAGGTGGTGGGGCGACGAACGAGCGGAGATGAGACTCCTCAGTCTGCAGTCATCTCTGGTTTGCAGGAGATTGAGGATAGAATAAAGGAATTGGAGGCAGAGAACCGGAAAAGTAAGAGGTTTGTTGATGGATGGGGAGAGAGATTGAAAGAGATAAGTGTGCTGATGAAGCAAGTGCGCGAACCGGGTGCAAGAGGGTCTTATCTCAAGGACTCAGAAAAGGCTGAGATGTATCGGTTGCACAAGGAAAACCCTGATGTTTACACTGTTGAGCGCCTGGCCAAGGATTATAGGATTATGAGGCAGAGAGTGCATGCCATTCTTTGGCTGAAGGAGCTTCAGGAGGAAGAGGAGAAGAAGCTTGGTCGTCCATTAGATGATTCTGTTGAGCTCCTGCTTGACACCTTTCCTGAATTTTTCAATTCCCATGACCGTGAATTCCATGTTGCATCACTTCCTTACAAACCGGACTTCAAAGTTATGCCAGAGGGTTGGGATGGTACCACCAGAGATTTGGATGAAGTCCACTATGAGATGTCCAAGAAGGAAGATGAGATGCTTTATCAAGAATTTGTCCAGAGGATGAACTTCAACAAAATGAAAATGAGAGGAGAGGTTTTCCACCACAAGTATAGTCGTCGTCGCTCTGCAGATGGGTGGAATTTCACGGTGGAGAAAATGGGACCCAAAGGAAAGCGTGGAGGTGGTGGTGGCTGGAAGTTTGTCAGCCTGCCAGATGGTTCGAGCCGACCACTGAATGAAAAGGAGAAAATGTATGTGAAACGAGAAACTCCACGCCATCGACGCAAGATCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bot1p MRP-L20

Protein Analysis

374

Amino Acids

43.07

Weight (kDa)

8.58

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-L20 PF12824 141 - 218 3e-06 Mitochondrial ribosomal protein subunit L20
Bot1p PF12298 169 - 277 2.9e-06 Eukaryotic mitochondrial regulator protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62270 AT5G62270
fragaria_vesca FvH4_3g26250 FvH4_3g26250
malus_domestica MD03G1229800.v1.1 MD11G1250300.v1.1
prunus_persica Prupe.4G179500_v2.0.a1
pyrus_communis pycom03g17740 pycom11g22150
rosa_chinensis RchiOBHm_Chr5g0048751
rosa_laevigata RLG00000034557
rosa_multiflora Rmu_sc0006382.1_g000016
rosa_roxburghii Rroxscaffold_1G00033070
rosa_rugosa Rorug05G0241000 Rorug05G0258100
rosa_samantha Rh5AG320800 Rh5CG356500 Rh5DG341800
rosa_wichuraiana Rw5G030140 Rw5G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 343
Acc65I GGTACC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 803
AccB7I CCANNNNNTGG 1 cut(s) 627
AccBSI CCGCTC 1 cut(s) 327
AccII CGCG 1 cut(s) 483
AciI CCGC 2 cut(s) 212, 327
AclWI GGATC 1 cut(s) 1108
AcoI YGGCCR 1 cut(s) 582
AcsI RAATTY 4 cut(s) 716, 740, 872, 964
AcuI CTGAAG 2 cut(s) 626, 653
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 3 cut(s) 212, 586, 627
AgsI TTSAA 6 cut(s) 45, 119, 451, 724, 778, 895
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 6 cut(s) 122, 130, 138, 640, 664, 694
AluI AGCT 6 cut(s) 122, 130, 138, 640, 664, 694
Alw21I GWGCWC 2 cut(s) 140, 696
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 1 cut(s) 1108
AlwNI CAGNNNCTG 1 cut(s) 10
AoxI GGCC 1 cut(s) 582
ApoI RAATTY 4 cut(s) 716, 740, 872, 964
Asp718I GGTACC 1 cut(s) 803
AspLEI GCGC 2 cut(s) 483, 579
AspS9I GGNCC 1 cut(s) 986
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 1 cut(s) 179
AvaII GGWCC 1 cut(s) 986
BalI TGGCCA 1 cut(s) 584
BanI GGYRCC 1 cut(s) 803
BanII GRGCYC 2 cut(s) 140, 696
BauI CACGAG 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 140, 696
BccI CCATC 9 cut(s) 164, 176, 293, 425, 429, 794, 950, 1034, 1110
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 1 cut(s) 581
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 348, 951
BfoI RGCGCY 1 cut(s) 580
Bme1390I CCNGG 2 cut(s) 489, 581
Bme18I GGWCC 1 cut(s) 986
BmgT120I GGNCC 1 cut(s) 986
BmiI GGNNCC 3 cut(s) 805, 987, 988
BmrFI CCNGG 2 cut(s) 489, 581
BmsI GCATC 3 cut(s) 242, 761, 849
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 285, 585
BsaWI WCCGGW 2 cut(s) 408, 769
BsaXI ACNNNNNCTCC 2 cut(s) 1061, 1091
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 586, 627
Bse1I ACTGG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 2 cut(s) 285, 585
BseGI GGATG 4 cut(s) 440, 805, 826, 891
BseLI CCNNNNNNNGG 3 cut(s) 212, 586, 627
BseMII CTCAG 3 cut(s) 356, 519, 531
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 3 cut(s) 330, 668, 927
Bsh1236I CGCG 1 cut(s) 483
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 1 cut(s) 803
BsiHKAI GWGCWC 2 cut(s) 140, 696
BsiSI CCGG 3 cut(s) 409, 488, 770
BslFI GGGAC 1 cut(s) 999
BslI CCNNNNNNNGG 3 cut(s) 212, 586, 627
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 999
BsnI GGCC 1 cut(s) 584
Bsp1286I GDGCHC 2 cut(s) 140, 696
Bsp143I GATC 1 cut(s) 1113
BspACI CCGC 2 cut(s) 212, 327
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 3 cut(s) 355, 520, 530
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 3 cut(s) 805, 987, 988
BspMAI CTGCAG 2 cut(s) 352, 955
BspPI GGATC 1 cut(s) 1108
BspQI GCTCTTC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 803
BsrBI CCGCTC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 96
BssECI CCNNGG 2 cut(s) 285, 585
BssMI GATC 1 cut(s) 1113
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 2 cut(s) 285, 585
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 5 cut(s) 17, 191, 571, 737, 973
Bst6I CTCTTC 2 cut(s) 80, 645
BstAPI GCANNNNNTGC 2 cut(s) 10, 613
BstC8I GCNNGC 2 cut(s) 618, 1034
BstDEI CTNAG 3 cut(s) 342, 517, 528
BstENI CCTNNNNNAGG 1 cut(s) 584
BstF5I GGATG 4 cut(s) 440, 805, 826, 891
BstFNI CGCG 1 cut(s) 483
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 2 cut(s) 483, 579
BstKTI GATC 1 cut(s) 1116
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 1113
BstMWI GCNNNNNNNGC 5 cut(s) 10, 472, 613, 637, 1107
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 620
BstSCI CCNGG 2 cut(s) 487, 579
BstSFI CTRYAG 2 cut(s) 348, 951
BstUI CGCG 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 1113
BstXI CCANNNNNNTGG 3 cut(s) 164, 795, 817
BstYI RGATCY 1 cut(s) 1113
BsuRI GGCC 1 cut(s) 584
BtsCI GGATG 4 cut(s) 440, 805, 826, 891
BtsIMutI CAGTG 3 cut(s) 196, 567, 1055
Cac8I GCNNGC 2 cut(s) 618, 1034
CaiI CAGNNNCTG 1 cut(s) 10
CfoI GCGC 2 cut(s) 483, 579
Cfr13I GGNCC 1 cut(s) 986
CseI GACGC 1 cut(s) 1116
Csp6I GTAC 1 cut(s) 804
CviAII CATG 4 cut(s) 617, 731, 746, 1122
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 3 cut(s) 342, 517, 528
DpnI GATC 1 cut(s) 1115
DpnII GATC 1 cut(s) 1113
DrdI GACNNNNNNGTC 1 cut(s) 343
DseDI GACNNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 2 cut(s) 80, 645
EarI CTCTTC 2 cut(s) 80, 645
Ecl136II GAGCTC 2 cut(s) 138, 694
Eco130I CCWWGG 2 cut(s) 285, 585
Eco24I GRGCYC 2 cut(s) 140, 696
Eco47I GGWCC 1 cut(s) 986
Eco53kI GAGCTC 2 cut(s) 138, 694
Eco57I CTGAAG 2 cut(s) 626, 653
EcoICRI GAGCTC 2 cut(s) 138, 694
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 2 cut(s) 285, 585
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 2 cut(s) 140, 696
ErhI CCWWGG 2 cut(s) 285, 585
FaeI CATG 4 cut(s) 620, 734, 749, 1125
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FaqI GGGAC 1 cut(s) 999
FatI CATG 4 cut(s) 616, 730, 745, 1121
FauI CCCGC 1 cut(s) 205
FokI GGATG 4 cut(s) 447, 812, 833, 898
FriOI GRGCYC 2 cut(s) 140, 696
GlaI GCGC 2 cut(s) 482, 578
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 1 cut(s) 584
HapII CCGG 3 cut(s) 409, 488, 770
HgaI GACGC 1 cut(s) 1116
HhaI GCGC 2 cut(s) 483, 579
Hin1II CATG 4 cut(s) 620, 734, 749, 1125
Hin6I GCGC 2 cut(s) 481, 577
HinP1I GCGC 2 cut(s) 481, 577
HindIII AAGCTT 3 cut(s) 120, 128, 662
HinfI GANTC 5 cut(s) 7, 254, 337, 515, 683
HpaII CCGG 3 cut(s) 409, 488, 770
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 2 cut(s) 565, 829
Hpy188I TCNGA 2 cut(s) 238, 520
Hpy188III TCNNGA 5 cut(s) 141, 644, 713, 869, 880
Hpy8I GTNNAC 2 cut(s) 565, 829
Hpy99I CGWCG 4 cut(s) 321, 945, 948, 1110
HpyAV CCTTC 2 cut(s) 628, 841
HpyCH4III ACNGT 5 cut(s) 17, 191, 571, 737, 973
HpyCH4IV ACGT 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 472, 613, 637, 1107
HpyF3I CTNAG 3 cut(s) 342, 517, 528
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 4 cut(s) 620, 734, 749, 1125
HspAI GCGC 2 cut(s) 481, 577
KflI GGGWCCC 1 cut(s) 986
KpnI GGTACC 1 cut(s) 807
Kzo9I GATC 1 cut(s) 1113
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 4 cut(s) 143, 289, 637, 699
LweI GCATC 3 cut(s) 242, 761, 849
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 185
MalI GATC 1 cut(s) 1115
MbiI CCGCTC 1 cut(s) 327
MboI GATC 1 cut(s) 1113
MboII GAAGA 4 cut(s) 97, 662, 670, 863
MflI RGATCY 1 cut(s) 1113
MhlI GDGCHC 2 cut(s) 140, 696
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 7 cut(s) 241, 392, 716, 724, 740, 872, 964
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 2 cut(s) 331, 509
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 235
MscI TGGCCA 1 cut(s) 584
MslI CAYNNNNRTG 2 cut(s) 297, 735
Msp20I TGGCCA 1 cut(s) 584
MspI CCGG 3 cut(s) 409, 488, 770
MspR9I CCNGG 2 cut(s) 489, 581
MvaI CCWGG 1 cut(s) 581
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 5 cut(s) 10, 472, 613, 637, 1107
NciI CCSGG 1 cut(s) 489
NdeII GATC 1 cut(s) 1113
NlaIII CATG 4 cut(s) 620, 734, 749, 1125
NlaIV GGNNCC 3 cut(s) 805, 987, 988
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 1 cut(s) 620
PaeI GCATGC 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 80
PfeI GAWTC 3 cut(s) 7, 254, 683
PflMI CCANNNNNTGG 1 cut(s) 627
PleI GAGTC 2 cut(s) 331, 509
PpsI GAGTC 2 cut(s) 331, 509
PpuMI RGGWCCY 1 cut(s) 986
Psp124BI GAGCTC 2 cut(s) 140, 696
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 805, 987, 988
PspPI GGNCC 1 cut(s) 986
PspPPI RGGWCCY 1 cut(s) 986
PstI CTGCAG 2 cut(s) 352, 955
PstNI CAGNNNCTG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 1113
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 2 cut(s) 297, 735
SacI GAGCTC 2 cut(s) 140, 696
SapI GCTCTTC 1 cut(s) 80
Sau3AI GATC 1 cut(s) 1113
Sau96I GGNCC 1 cut(s) 986
SchI GAGTC 2 cut(s) 331, 509
ScrFI CCNGG 2 cut(s) 489, 581
SduI GDGCHC 2 cut(s) 140, 696
SfaNI GCATC 3 cut(s) 242, 761, 849
SfcI CTRYAG 2 cut(s) 348, 951
SinI GGWCC 1 cut(s) 986
SmiMI CAYNNNNRTG 2 cut(s) 297, 735
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SphI GCATGC 1 cut(s) 620
Sse9I AATT 7 cut(s) 241, 392, 716, 724, 740, 872, 964
SsiI CCGC 2 cut(s) 212, 327
SstI GAGCTC 2 cut(s) 140, 696
StyD4I CCNGG 2 cut(s) 487, 579
StyI CCWWGG 2 cut(s) 285, 585
TaaI ACNGT 5 cut(s) 17, 191, 571, 737, 973
TaiI ACGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 1046, 1105
TasI AATT 7 cut(s) 241, 392, 716, 724, 740, 872, 964
TfiI GAWTC 3 cut(s) 7, 254, 683
TscAI CASTG 3 cut(s) 196, 574, 1062
TspDTI ATGAA 5 cut(s) 485, 837, 902, 917, 1077
TspRI CASTG 3 cut(s) 196, 574, 1062
Van91I CCANNNNNTGG 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 986
XagI CCTNNNNNAGG 1 cut(s) 584
XapI RAATTY 4 cut(s) 716, 740, 872, 964
XceI RCATGY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.