AT5G62290

Nucleotide-sensitive chloride conductance regulator (ICln) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008009
Physical Location & Seq
Forward (+)
25019175 .. 25021131
1957 bp
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UTR
Exon/CDS
Intron
AT5G62290.2

Sequence Viewer

Length: 690 bp
ATGGTGGCTGGTCTAAGAGATTTCACGCTCAGAACCGAAGATGGCTCCGGGAAACCGGTACTGGATGAATCTAACGGCGAGGAACTTATGCACGTTCAGACTTCCGTCGCCGTCGCTCTCGGAAACCGTCCCATTGAGTCTCCCGGAACTCTCTACATCACTTCCAGGAAATTAATTTGGCTGAGCGATGTAGACATGGCTAAAGGATACGCAGTTGATTTCTTATCAATATCGCTTCACGCTGTTTCTAGAGATCCTGAGGCTTATTCATCTCCTTGTATATATACTCAGATTGAAGTTGAAGAAGATGAGGATGATGAGTCTGATTCAGAATCCACTGAAGTTTTGGACTTATCAAAGATCAGAGAGATGAGGCTTGTTCCTTCTGACTCTACCCAGTTGGAGACGCTGTTTGATGTATTCTGCGAGTGTGCTGAGCTGAATCCTGAACCAGTTCAAGAAGAAGAAGAAGAAAGCGGACATAATTGGGTGTTTAGTGCTGATCAGATGGATGTTCGTGGAGGAGATGATGATGCAGAGTGGCAAATATCTCAGAGTCCGACTAGTGTAATTGGTCATTCCAATGGAGATGAAGGTCTTAATCAGCCTATGCTTGAGCTCCAAATCAATGATCAGAGGTTTGAGGATGCTGAAGAGATGGTTCATGAAAGTGAGACCAAAGATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

229

Amino Acids

25.6

Weight (kDa)

4.05

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voldacs PF03517 49 - 161 3.6e-27 Regulator of volume decrease after cellular swelling
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 192
AciI CCGC 1 cut(s) 477
AclWI GGATC 1 cut(s) 248
AcuI CTGAAG 2 cut(s) 360, 672
AfaI GTAC 1 cut(s) 60
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 3 cut(s) 296, 302, 458
AhlI ACTAGT 1 cut(s) 563
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 2 cut(s) 439, 619
AluI AGCT 2 cut(s) 439, 619
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 3 cut(s) 144, 398, 668
AlwI GGATC 1 cut(s) 248
AseI ATTAAT 1 cut(s) 173
AsiGI ACCGGT 1 cut(s) 55
Asp700I GAANNNNTTC 1 cut(s) 453
AsuC2I CCSGG 2 cut(s) 49, 144
AxyI CCTNAGG 1 cut(s) 258
BanII GRGCYC 1 cut(s) 621
Bbv12I GWGCWC 1 cut(s) 621
BccI CCATC 3 cut(s) 35, 502, 652
BceAI ACGGC 2 cut(s) 91, 95
BciT130I CCWGG 1 cut(s) 166
BciVI GTATCC 1 cut(s) 200
BclI TGATCA 2 cut(s) 502, 631
BcnI CCSGG 2 cut(s) 49, 144
BcoDI GTCTC 3 cut(s) 144, 398, 668
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 3 cut(s) 249, 564, 688
BfuI GTATCC 1 cut(s) 200
BlpI GCTNAGC 2 cut(s) 182, 435
Bme1390I CCNGG 3 cut(s) 49, 144, 166
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 3 cut(s) 49, 144, 166
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 2 cut(s) 523, 637
BmuI ACTGGG 1 cut(s) 391
BoxI GACNNNNGTC 1 cut(s) 104
Bpu1102I GCTNAGC 2 cut(s) 182, 435
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 2 cut(s) 49, 144
BsaI GGTCTC 1 cut(s) 668
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 66, 397, 452
Bse21I CCTNAGG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 166
BseGI GGATG 4 cut(s) 70, 319, 517, 652
BseMII CTCAG 6 cut(s) 43, 173, 249, 302, 426, 566
BseNI ACTGG 3 cut(s) 66, 397, 452
BseRI GAGGAG 1 cut(s) 537
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 3 cut(s) 48, 56, 144
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 3 cut(s) 144, 398, 668
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 114
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 5 cut(s) 253, 360, 502, 631, 682
Bsp1720I GCTNAGC 2 cut(s) 182, 435
BspACI CCGC 1 cut(s) 477
BspCNI CTCAG 6 cut(s) 42, 174, 250, 301, 427, 565
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 66, 397, 452
BssAI RCCGGY 1 cut(s) 55
BssMI GATC 5 cut(s) 253, 360, 502, 631, 682
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 1 cut(s) 128
Bst6I CTCTTC 1 cut(s) 648
BstDEI CTNAG 7 cut(s) 14, 29, 182, 258, 288, 435, 552
BstF5I GGATG 4 cut(s) 70, 319, 517, 652
BstKTI GATC 5 cut(s) 256, 363, 505, 634, 685
BstMAI GTCTC 3 cut(s) 144, 398, 668
BstMBI GATC 5 cut(s) 253, 360, 502, 631, 682
BstNI CCWGG 1 cut(s) 166
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 3 cut(s) 47, 142, 164
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 258
BsuI GTATCC 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 4 cut(s) 70, 319, 517, 652
BtsIMutI CAGTG 1 cut(s) 336
CciI TCATGA 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 55
CseI GACGC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 59
CspAI ACCGGT 1 cut(s) 55
CviAII CATG 2 cut(s) 196, 665
CviJI RGCY 9 cut(s) 8, 45, 181, 200, 263, 376, 439, 607, 619
CviKI_1 RGCY 9 cut(s) 8, 45, 181, 200, 263, 376, 439, 607, 619
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 7 cut(s) 14, 29, 182, 258, 288, 435, 552
DpnI GATC 5 cut(s) 255, 362, 504, 633, 684
DpnII GATC 5 cut(s) 253, 360, 502, 631, 682
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Ecl136II GAGCTC 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 668
Eco53kI GAGCTC 1 cut(s) 619
Eco57I CTGAAG 2 cut(s) 360, 672
Eco81I CCTNAGG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 164
EcoT38I GRGCYC 1 cut(s) 621
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 2 cut(s) 199, 668
FaiI YATR 8 cut(s) 89, 197, 281, 283, 285, 483, 611, 666
FaqI GGGAC 1 cut(s) 114
FatI CATG 2 cut(s) 195, 664
FbaI TGATCA 2 cut(s) 502, 631
FblI GTMKAC 1 cut(s) 192
FokI GGATG 4 cut(s) 77, 326, 524, 659
FriOI GRGCYC 1 cut(s) 621
FspBI CTAG 3 cut(s) 249, 564, 688
HapII CCGG 3 cut(s) 48, 56, 144
HgaI GACGC 1 cut(s) 415
Hin1II CATG 2 cut(s) 199, 668
HinfI GANTC 8 cut(s) 68, 137, 320, 326, 332, 389, 442, 556
HpaII CCGG 3 cut(s) 48, 56, 144
Hpy166II GTNNAC 1 cut(s) 193
Hpy188III TCNNGA 5 cut(s) 249, 257, 446, 458, 665
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 2 cut(s) 110, 116
HpyAV CCTTC 2 cut(s) 393, 587
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 2 cut(s) 91, 536
HpyF3I CTNAG 7 cut(s) 14, 29, 182, 258, 288, 435, 552
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 2 cut(s) 199, 668
Ksp22I TGATCA 2 cut(s) 502, 631
Kzo9I GATC 5 cut(s) 253, 360, 502, 631, 682
LmnI GCTCC 2 cut(s) 50, 624
LweI GCATC 2 cut(s) 523, 637
MaeI CTAG 3 cut(s) 249, 564, 688
MaeII ACGT 1 cut(s) 93
MalI GATC 5 cut(s) 255, 362, 504, 633, 684
MboI GATC 5 cut(s) 253, 360, 502, 631, 682
MboII GAAGA 8 cut(s) 50, 314, 317, 473, 476, 479, 482, 665
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 4 cut(s) 170, 174, 484, 570
MlyI GAGTC 4 cut(s) 146, 329, 383, 565
MmeI TCCRAC 2 cut(s) 381, 584
MnlI CCTC 7 cut(s) 73, 253, 304, 366, 515, 630, 637
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 2 cut(s) 173, 600
MslI CAYNNNNRTG 2 cut(s) 582, 669
MspI CCGG 3 cut(s) 48, 56, 144
MspR9I CCNGG 3 cut(s) 49, 144, 166
MvaI CCWGG 1 cut(s) 166
NciI CCSGG 2 cut(s) 49, 144
NdeII GATC 5 cut(s) 253, 360, 502, 631, 682
NlaIII CATG 2 cut(s) 199, 668
NlaIV GGNNCC 1 cut(s) 46
PagI TCATGA 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 4 cut(s) 68, 326, 332, 442
PfoI TCCNGGA 3 cut(s) 47, 142, 164
PinAI ACCGGT 1 cut(s) 55
PleI GAGTC 4 cut(s) 145, 328, 383, 564
PpsI GAGTC 4 cut(s) 145, 328, 383, 564
PshAI GACNNNNGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 2 cut(s) 582, 669
SacI GAGCTC 1 cut(s) 621
SaqAI TTAA 2 cut(s) 173, 600
Sau3AI GATC 5 cut(s) 253, 360, 502, 631, 682
SchI GAGTC 4 cut(s) 146, 329, 383, 565
ScrFI CCNGG 3 cut(s) 49, 144, 166
SduI GDGCHC 1 cut(s) 621
SetI ASST 5 cut(s) 96, 441, 598, 621, 641
SfaNI GCATC 2 cut(s) 523, 637
SmiMI CAYNNNNRTG 2 cut(s) 582, 669
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SpeI ACTAGT 1 cut(s) 563
Sse9I AATT 4 cut(s) 170, 174, 484, 570
SsiI CCGC 1 cut(s) 477
SspMI CTAG 3 cut(s) 249, 564, 688
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 47, 142, 164
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 1 cut(s) 96
TasI AATT 4 cut(s) 170, 174, 484, 570
TfiI GAWTC 4 cut(s) 68, 326, 332, 442
Tru1I TTAA 2 cut(s) 173, 600
Tru9I TTAA 2 cut(s) 173, 600
TscAI CASTG 1 cut(s) 343
TspDTI ATGAA 5 cut(s) 81, 258, 606, 653, 681
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 343
VspI ATTAAT 1 cut(s) 173
XbaI TCTAGA 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 249, 564, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.