RLG00000026703

Chloride conductance regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008009
Physical Location & Seq
Forward (+)
5172396 .. 5174190
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026703

Sequence Viewer

Length: 678 bp
ATGGTGGAAGGGATTAGAGACTTCACTGCTATAGTCGAGAGAGATGGTGTTCACCAACCTGTTCTGGATGCACAGAATGGCGAGGAGCTGATGCATGTTCAGCCTGGTGTTTCTATTGTCCTCGGTAGTCACCCTCCTGAGTCCCCTGGCACTCTCTATATCTCCACCAGGCAAGTGATTTGGTTGAGTGACACTAACAGGGCCAAAGGTTATGCGGTGGACTTCCTGTCTATATCACTGCATGCTGTATCCAGGGACCCAGAGGCGTACACCTCTCCTTGCATATACGCTCAGATTGAAACTGGAGCTGATGAAGATGAGTCAGAGGGCTCAGATTCAGAATGCAATGGCGTTTTAGATCTGTCCAAGATCACAGAAATGAGGCTTGTTCCTTCGGATCCTAACCAATTGGATTCTTTGTTTGATGTATTTTGTGAGTGTGCTGAGCTGAATCCTGAACCAACTGAAGAAAATGAAGAAGAAGAGCATAATTGGATATTTAGTGCTGACCAGTTGGAAGATGAGGCAACAGGAGAAGATTCTGAGCCTTCAATTGCAATTGGGCAATCTAATGGGGATCATGACCTAGCTCGTACTGTGCTTGAGCTTCAGATCAATGATCAACGCTTTGAGGATGCAGAAGAGATGGAGCATGAGACTGACAGTGCACAGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

226

Amino Acids

24.95

Weight (kDa)

4.05

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voldacs PF03517 50 - 176 3.1e-27 Regulator of volume decrease after cellular swelling
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 215
AclWI GGATC 3 cut(s) 392, 405, 585
AcuI CTGAAG 2 cut(s) 486, 593
AfaI GTAC 2 cut(s) 269, 595
AfeI AGCGCT 1 cut(s) 674
AgsI TTSAA 2 cut(s) 299, 552
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 4 cut(s) 103, 145, 167, 251
AluBI AGCT 5 cut(s) 88, 308, 448, 590, 607
AluI AGCT 5 cut(s) 88, 308, 448, 590, 607
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 2 cut(s) 12, 650
Alw44I GTGCAC 1 cut(s) 666
AlwI GGATC 3 cut(s) 392, 405, 585
Aor51HI AGCGCT 1 cut(s) 674
AoxI GGCC 1 cut(s) 201
ApaLI GTGCAC 1 cut(s) 666
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 2 cut(s) 201, 256
AsuHPI GGTGA 2 cut(s) 44, 122
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 670
BamHI GGATCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 670
BccI CCATC 2 cut(s) 38, 640
BciT130I CCWGG 4 cut(s) 105, 147, 169, 253
BciVI GTATCC 1 cut(s) 259
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 12, 650
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 676
BfuI GTATCC 1 cut(s) 259
BglII AGATCT 1 cut(s) 358
BlpI GCTNAGC 1 cut(s) 444
Bme1390I CCNGG 4 cut(s) 105, 147, 169, 253
Bme18I GGWCC 1 cut(s) 256
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 201, 256
BmiI GGNNCC 3 cut(s) 257, 258, 399
BmrFI CCNGG 4 cut(s) 105, 147, 169, 253
BmsI GCATC 3 cut(s) 58, 81, 625
BpmI CTGGAG 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 623
BsaJI CCNNGG 3 cut(s) 121, 145, 252
Bse1I ACTGG 2 cut(s) 307, 511
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 4 cut(s) 105, 147, 169, 253
BseDI CCNNGG 3 cut(s) 121, 145, 252
BseGI GGATG 2 cut(s) 73, 640
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 5 cut(s) 129, 305, 345, 435, 534
BseNI ACTGG 2 cut(s) 307, 511
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 670
BshFI GGCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 670
BslFI GGGAC 2 cut(s) 127, 269
BsmAI GTCTC 2 cut(s) 12, 650
BsmFI GGGAC 2 cut(s) 127, 269
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 332, 670
Bsp143I GATC 6 cut(s) 358, 369, 397, 577, 612, 619
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 1 cut(s) 215
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 5 cut(s) 130, 304, 344, 436, 535
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 3 cut(s) 257, 258, 399
BspPI GGATC 3 cut(s) 392, 405, 585
BspQI GCTCTTC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 2 cut(s) 307, 511
BssECI CCNNGG 3 cut(s) 121, 145, 252
BssMI GATC 6 cut(s) 358, 369, 397, 577, 612, 619
Bst2UI CCWGG 4 cut(s) 105, 147, 169, 253
Bst4CI ACNGT 2 cut(s) 598, 665
Bst6I CTCTTC 2 cut(s) 477, 636
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 5 cut(s) 138, 291, 331, 444, 543
BstF5I GGATG 2 cut(s) 73, 640
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 6 cut(s) 361, 372, 400, 580, 615, 622
BstMAI GTCTC 2 cut(s) 12, 650
BstMBI GATC 6 cut(s) 358, 369, 397, 577, 612, 619
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 4 cut(s) 105, 147, 169, 253
BstNSI RCATGY 2 cut(s) 98, 245
BstSCI CCNGG 4 cut(s) 103, 145, 167, 251
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 670
BstX2I RGATCY 2 cut(s) 358, 397
BstYI RGATCY 2 cut(s) 358, 397
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 2 cut(s) 73, 640
BtsI GCAGTG 2 cut(s) 24, 236
BtsIMutI CAGTG 3 cut(s) 24, 236, 670
Cac8I GCNNGC 1 cut(s) 243
CciI TCATGA 1 cut(s) 580
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 2 cut(s) 201, 256
Csp6I GTAC 2 cut(s) 268, 594
CviAII CATG 4 cut(s) 95, 242, 581, 653
CviQI GTAC 2 cut(s) 268, 594
DdeI CTNAG 5 cut(s) 138, 291, 331, 444, 543
DpnI GATC 6 cut(s) 360, 371, 399, 579, 614, 621
DpnII GATC 6 cut(s) 358, 369, 397, 577, 612, 619
DriI GACNNNNNGTC 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 477, 636
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 2 cut(s) 477, 636
Eco24I GRGCYC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 256
Eco47III AGCGCT 1 cut(s) 674
Eco57I CTGAAG 2 cut(s) 486, 593
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 4 cut(s) 103, 145, 167, 251
EcoT22I ATGCAT 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 332
FaeI CATG 4 cut(s) 98, 245, 584, 656
FaqI GGGAC 2 cut(s) 127, 269
FatI CATG 4 cut(s) 94, 241, 580, 652
FbaI TGATCA 1 cut(s) 619
FokI GGATG 2 cut(s) 80, 647
FriOI GRGCYC 1 cut(s) 332
FspBI CTAG 1 cut(s) 587
GlaI GCGC 1 cut(s) 674
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 1 cut(s) 203
HhaI GCGC 1 cut(s) 675
Hin1II CATG 4 cut(s) 98, 245, 584, 656
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HinfI GANTC 6 cut(s) 140, 320, 335, 413, 451, 539
HphI GGTGA 2 cut(s) 44, 122
Hpy166II GTNNAC 4 cut(s) 52, 220, 270, 668
Hpy188I TCNGA 7 cut(s) 294, 325, 334, 340, 397, 544, 612
Hpy188III TCNNGA 5 cut(s) 37, 65, 137, 455, 581
Hpy8I GTNNAC 4 cut(s) 52, 220, 270, 668
HpyAV CCTTC 2 cut(s) 402, 558
HpyCH4III ACNGT 2 cut(s) 598, 665
HpyCH4V TGCA 8 cut(s) 71, 94, 241, 282, 345, 557, 638, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 5 cut(s) 138, 291, 331, 444, 543
Hsp92II CATG 4 cut(s) 98, 245, 584, 656
HspAI GCGC 1 cut(s) 673
KflI GGGWCCC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 6 cut(s) 358, 369, 397, 577, 612, 619
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 3 cut(s) 85, 305, 649
LweI GCATC 3 cut(s) 58, 81, 625
MaeI CTAG 1 cut(s) 587
MaeIII GTNAC 2 cut(s) 128, 188
MalI GATC 6 cut(s) 360, 371, 399, 579, 614, 621
MboI GATC 6 cut(s) 358, 369, 397, 577, 612, 619
MboII GAAGA 8 cut(s) 326, 479, 488, 491, 494, 530, 548, 653
MfeI CAATTG 3 cut(s) 407, 552, 558
MflI RGATCY 2 cut(s) 358, 397
MhlI GDGCHC 2 cut(s) 332, 670
MluCI AATT 4 cut(s) 407, 490, 552, 558
MlyI GAGTC 2 cut(s) 149, 329
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 9 cut(s) 76, 131, 144, 256, 283, 319, 375, 517, 625
Mph1103I ATGCAT 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 377
MspR9I CCNGG 4 cut(s) 105, 147, 169, 253
MunI CAATTG 3 cut(s) 407, 552, 558
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 4 cut(s) 105, 147, 169, 253
MwoI GCNNNNNNNGC 1 cut(s) 100
NdeII GATC 6 cut(s) 358, 369, 397, 577, 612, 619
NlaIII CATG 4 cut(s) 98, 245, 584, 656
NlaIV GGNNCC 3 cut(s) 257, 258, 399
NmuCI GTSAC 2 cut(s) 128, 188
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 2 cut(s) 98, 245
PaeI GCATGC 1 cut(s) 245
PagI TCATGA 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 477
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 4 cut(s) 335, 413, 451, 539
PleI GAGTC 2 cut(s) 148, 328
PpsI GAGTC 2 cut(s) 148, 328
PpuMI RGGWCCY 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 4 cut(s) 103, 145, 167, 251
PspGI CCWGG 4 cut(s) 103, 145, 167, 251
PspN4I GGNNCC 3 cut(s) 257, 258, 399
PspPI GGNCC 2 cut(s) 201, 256
PspPPI RGGWCCY 1 cut(s) 256
PsuI RGATCY 2 cut(s) 358, 397
RsaI GTAC 2 cut(s) 269, 595
RsaNI GTAC 2 cut(s) 268, 594
RseI CAYNNNNRTG 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 477
Sau3AI GATC 6 cut(s) 358, 369, 397, 577, 612, 619
Sau96I GGNCC 2 cut(s) 201, 256
SchI GAGTC 2 cut(s) 149, 329
ScrFI CCNGG 4 cut(s) 105, 147, 169, 253
SduI GDGCHC 2 cut(s) 332, 670
SetI ASST 9 cut(s) 61, 90, 211, 275, 310, 450, 588, 592, 609
SfaNI GCATC 3 cut(s) 58, 81, 625
SfcI CTRYAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SphI GCATGC 1 cut(s) 245
Sse9I AATT 4 cut(s) 407, 490, 552, 558
SsiI CCGC 1 cut(s) 215
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 4 cut(s) 103, 145, 167, 251
TaaI ACNGT 2 cut(s) 598, 665
TaqI TCGA 1 cut(s) 36
TasI AATT 4 cut(s) 407, 490, 552, 558
TfiI GAWTC 4 cut(s) 335, 413, 451, 539
TscAI CASTG 3 cut(s) 31, 243, 670
TseFI GTSAC 2 cut(s) 128, 188
Tsp45I GTSAC 2 cut(s) 128, 188
TspDTI ATGAA 2 cut(s) 327, 489
TspRI CASTG 3 cut(s) 31, 243, 670
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 1 cut(s) 256
XceI RCATGY 2 cut(s) 98, 245
XspI CTAG 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.