MD07G1257000.v1.1

Chloride conductance regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008009
Physical Location & Seq
Reverse (-)
32344626 .. 32347155
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1257000.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGTGGCAGGGCTTAAAGAATTCACTGCAAGAGTTGAAAGAAACGGTGTTTATGAACCAGTTCTGGATGCGGAGAACGGTGAGGAGCTGATGCGCGTTCAGCCTGGTGTTTCTATTGTCCTCGGTAATCGACCCCCTGAGTCCCCGGGCACCCTCTACATCTCCACCAAGCAAGTGGTTTGGTTGAGTGACGTTGATAGGGAGAAGGGTTATGCTGTGGACTTCTTGTCTATATCACTGCACGCTGTGTCCAGAGACCCGGAGGCCTACACCTCTCCTTGTATTTACACTCAGATTGAAACTGAGGATGACGAGGACGAGTCTGACGGTTCCGATTCAGAATCCAATGGTGTCTTAGATTTATCCAAGATCACAGAAATGAGACTTGTTCCTTCAGATCCTAACCAATTGGATTCTCTGTTTGAGGTGTTTTGCGAGTGTGCAGAGCTTAATCCTGAACCAATTGAAGGGGAAGAAGAGCATAACTGGATTTTTAGTGCCGATCAATTGGAAGACGAGGCAGTTGGGGACGATTCTGAGTGGCCTGTTCCTCAAAATCTTTCAAACCCAATTGGTCAATCGAACGGGGATCATGACCTAGCTCGTACTGTGCTTGAGCTTCAGATCAACGATCAGCGCTTTGAGGATGCCGAAGAAATGGAGCATGAGAGCGACAGTGATCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

230

Amino Acids

25.64

Weight (kDa)

4.05

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voldacs PF03517 50 - 174 4.5e-26 Regulator of volume decrease after cellular swelling
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccII CGCG 1 cut(s) 96
AciI CCGC 1 cut(s) 71
AclWI GGATC 2 cut(s) 392, 597
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 2 cut(s) 378, 605
AdeI CACNNNGTG 1 cut(s) 247
AfaI GTAC 1 cut(s) 607
AfeI AGCGCT 1 cut(s) 638
AfiI CCNNNNNNNGG 1 cut(s) 467
AgsI TTSAA 4 cut(s) 38, 299, 467, 564
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 4 cut(s) 88, 448, 602, 619
AluI AGCT 4 cut(s) 88, 448, 602, 619
Alw26I GTCTC 2 cut(s) 249, 376
AlwI GGATC 2 cut(s) 392, 597
Ama87I CYCGRG 1 cut(s) 145
Aor51HI AGCGCT 1 cut(s) 638
AoxI GGCC 2 cut(s) 264, 542
ApoI RAATTY 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 60
AspLEI GCGC 2 cut(s) 96, 639
AsuC2I CCSGG 3 cut(s) 146, 147, 260
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 519
BciT130I CCWGG 1 cut(s) 105
BcnI CCSGG 3 cut(s) 146, 147, 260
BcoDI GTCTC 2 cut(s) 249, 376
BfaI CTAG 1 cut(s) 599
BfoI RGCGCY 1 cut(s) 640
Bme1390I CCNGG 4 cut(s) 105, 146, 147, 260
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 151, 331
BmrFI CCNGG 4 cut(s) 105, 146, 147, 260
BmsI GCATC 3 cut(s) 58, 81, 637
BpiI GAAGAC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 3 cut(s) 146, 147, 260
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 121, 144, 145
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 59, 491
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 121, 144, 145
BseGI GGATG 3 cut(s) 73, 313, 652
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 4 cut(s) 129, 294, 305, 528
BseNI ACTGG 2 cut(s) 59, 491
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 152
BsgI GTGCAG 2 cut(s) 224, 462
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 266, 544
BshNI GGYRCC 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 2 cut(s) 146, 260
BslFI GGGAC 2 cut(s) 127, 542
BslI CCNNNNNNNGG 1 cut(s) 467
BsmAI GTCTC 2 cut(s) 249, 376
BsmFI GGGAC 2 cut(s) 127, 542
BsnI GGCC 2 cut(s) 266, 544
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 266, 544
BspCNI CTCAG 4 cut(s) 130, 295, 304, 529
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 151, 331
BspPI GGATC 2 cut(s) 392, 597
BspQI GCTCTTC 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 249
BsrI ACTGG 2 cut(s) 59, 491
BssECI CCNNGG 3 cut(s) 121, 144, 145
BssMI GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 5 cut(s) 47, 80, 329, 610, 677
Bst6I CTCTTC 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 5 cut(s) 138, 291, 303, 355, 537
BstF5I GGATG 3 cut(s) 73, 313, 652
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 2 cut(s) 96, 639
BstKTI GATC 7 cut(s) 372, 400, 505, 592, 627, 634, 682
BstMAI GTCTC 2 cut(s) 249, 376
BstMBI GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 4 cut(s) 103, 144, 145, 258
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 2 cut(s) 266, 544
BtsCI GGATG 3 cut(s) 73, 313, 652
BtsI GCAGTG 2 cut(s) 24, 236
BtsIMutI CAGTG 3 cut(s) 24, 236, 682
Cac8I GCNNGC 1 cut(s) 243
CciI TCATGA 1 cut(s) 592
CfoI GCGC 2 cut(s) 96, 639
Cfr9I CCCGGG 1 cut(s) 145
Csp6I GTAC 1 cut(s) 606
CviAII CATG 2 cut(s) 593, 665
CviJI RGCY 8 cut(s) 13, 88, 103, 266, 448, 544, 602, 619
CviKI_1 RGCY 8 cut(s) 13, 88, 103, 266, 448, 544, 602, 619
CviQI GTAC 1 cut(s) 606
DdeI CTNAG 5 cut(s) 138, 291, 303, 355, 537
DpnI GATC 7 cut(s) 371, 399, 504, 591, 626, 633, 681
DpnII GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
DraIII CACNNNGTG 1 cut(s) 247
DriI GACNNNNNGTC 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 471
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 249
Eco47III AGCGCT 1 cut(s) 638
Eco57I CTGAAG 2 cut(s) 378, 605
Eco88I CYCGRG 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 103
FaeI CATG 2 cut(s) 596, 668
FaiI YATR 6 cut(s) 54, 213, 233, 483, 594, 666
FaqI GGGAC 2 cut(s) 127, 542
FatI CATG 2 cut(s) 592, 664
FokI GGATG 3 cut(s) 80, 320, 659
FspBI CTAG 1 cut(s) 599
GlaI GCGC 2 cut(s) 95, 638
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 2 cut(s) 266, 544
HapII CCGG 2 cut(s) 146, 260
HhaI GCGC 2 cut(s) 96, 639
Hin1II CATG 2 cut(s) 596, 668
Hin6I GCGC 2 cut(s) 94, 637
HinP1I GCGC 2 cut(s) 94, 637
HinfI GANTC 6 cut(s) 140, 320, 335, 341, 413, 533
HpaII CCGG 2 cut(s) 146, 260
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 7 cut(s) 294, 325, 334, 340, 397, 538, 624
Hpy188III TCNNGA 4 cut(s) 65, 252, 455, 593
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 199, 402, 461
HpyCH4III ACNGT 5 cut(s) 47, 80, 329, 610, 677
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 29, 241, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 5 cut(s) 138, 291, 303, 355, 537
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 596, 668
HspAI GCGC 2 cut(s) 94, 637
Kzo9I GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 2 cut(s) 85, 661
LweI GCATC 3 cut(s) 58, 81, 637
MaeI CTAG 1 cut(s) 599
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 188
MalI GATC 7 cut(s) 371, 399, 504, 591, 626, 633, 681
MboI GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
MboII GAAGA 4 cut(s) 485, 488, 524, 665
MfeI CAATTG 4 cut(s) 407, 462, 506, 570
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 5 cut(s) 20, 407, 462, 506, 570
MlyI GAGTC 2 cut(s) 149, 329
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 3 cut(s) 15, 450, 688
MslI CAYNNNNRTG 1 cut(s) 377
MspI CCGG 2 cut(s) 146, 260
MspR9I CCNGG 4 cut(s) 105, 146, 147, 260
MunI CAATTG 4 cut(s) 407, 462, 506, 570
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 3 cut(s) 146, 147, 260
NdeII GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
NlaIII CATG 2 cut(s) 596, 668
NlaIV GGNNCC 2 cut(s) 151, 331
NmuCI GTSAC 1 cut(s) 188
PagI TCATGA 1 cut(s) 592
PceI AGGCCT 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 4 cut(s) 335, 341, 413, 533
PleI GAGTC 2 cut(s) 148, 328
PpsI GAGTC 2 cut(s) 148, 328
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 151, 331
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 1 cut(s) 607
RsaNI GTAC 1 cut(s) 606
RseI CAYNNNNRTG 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 3 cut(s) 15, 450, 688
Sau3AI GATC 7 cut(s) 369, 397, 502, 589, 624, 631, 679
SchI GAGTC 2 cut(s) 149, 329
ScrFI CCNGG 4 cut(s) 105, 146, 147, 260
SduI GDGCHC 1 cut(s) 152
SetI ASST 8 cut(s) 90, 195, 275, 429, 450, 600, 604, 621
SfaNI GCATC 3 cut(s) 58, 81, 637
SmaI CCCGGG 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 5 cut(s) 20, 407, 462, 506, 570
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 1 cut(s) 71
SspMI CTAG 1 cut(s) 599
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 4 cut(s) 103, 144, 145, 258
TaaI ACNGT 5 cut(s) 47, 80, 329, 610, 677
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 130, 581
TasI AATT 5 cut(s) 20, 407, 462, 506, 570
TfiI GAWTC 4 cut(s) 335, 341, 413, 533
Tru1I TTAA 3 cut(s) 15, 450, 688
Tru9I TTAA 3 cut(s) 15, 450, 688
TscAI CASTG 3 cut(s) 31, 243, 682
TseFI GTSAC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 69
TspMI CCCGGG 1 cut(s) 145
TspRI CASTG 3 cut(s) 31, 243, 682
XapI RAATTY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmaI CCCGGG 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.