MD01G1184800.v1.1

Chloride conductance regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008009
Physical Location & Seq
Reverse (-)
28403278 .. 28405721
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1184800.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGTAGCAGGGATTAAAGAATTCACTGCTCGAGTTGAAAGAAACGGTGTTGATGAACCAGTTTTGGATGCGGTGAATGGTGAGGAGCTGATGCATGTTCGGCCTGGTGTTTCTATTGTCCTCGGCAATCAGCCCCCTGAGTTCCCTGGCACTCTATATATCTCCACCAAGCAAGTGATTTGGTTGAGTGACACTGACAAGGCGAAAGGTTATGCTTTGGACTTCTTGTCTATATCACTGCACGCTGTATCCAGAGACCCAGAGGCCTACACCTCTCCTTGTTTATACACTCAGATTGAAACTGAGGATGACGAGGATGAGTCTGATGGTTTGGATTCAGAACGCAATGGTGACTTAGATTTATCCAAGGTCACAGAGATGAGGCTCGTTCCTTCAGATCCTAACCAGTTGGATTCTCTGTTTGAGGTATTTTGTGAGTGTGCTGAGCTTAATCCAGAACCAATTCAAGGGGAAGAAGAGCATAATTGGATTTTTAGCGCCGATCAGTTGGAAGATGAGGCAGTTGAGGACGATTCTGAGTGGCCTGTTCCTCAAAATCCTTCAAACTCAATTGGTCAATCTAACGGGGATCATGACCTAGCTCGTACTGTGCTTGAGCTTCAGATCAACGATCAACGATTTGAGGATGCAGAAGAAATGGAGCATGAAACCGACAGTGGCCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

230

Amino Acids

25.65

Weight (kDa)

4.05

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voldacs PF03517 50 - 176 2.7e-26 Regulator of volume decrease after cellular swelling
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 71, 682
AclWI GGATC 2 cut(s) 392, 597
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 2 cut(s) 378, 605
AfaI GTAC 1 cut(s) 607
AfiI CCNNNNNNNGG 1 cut(s) 467
AgsI TTSAA 4 cut(s) 38, 299, 467, 564
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 103, 145
AluBI AGCT 4 cut(s) 88, 448, 602, 619
AluI AGCT 4 cut(s) 88, 448, 602, 619
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 2 cut(s) 392, 597
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 4 cut(s) 101, 264, 542, 679
ApoI RAATTY 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 500
AsuHPI GGTGA 3 cut(s) 85, 92, 362
AvaI CYCGRG 1 cut(s) 30
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 105, 147
BciVI GTATCC 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 1 cut(s) 599
BfoI RGCGCY 1 cut(s) 501
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 1 cut(s) 682
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 683
Bme1390I CCNGG 2 cut(s) 105, 147
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 105, 147
BmsI GCATC 3 cut(s) 58, 81, 637
Bpu1102I GCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 121, 145, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 59, 406
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 2 cut(s) 105, 147
BseDI CCNNGG 3 cut(s) 121, 145, 366
BseGI GGATG 4 cut(s) 73, 313, 322, 652
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 5 cut(s) 129, 294, 305, 435, 528
BseNI ACTGG 2 cut(s) 59, 406
BseRI GAGGAG 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 4 cut(s) 103, 266, 544, 681
BsiHKCI CYCGRG 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 467
BsmAI GTCTC 1 cut(s) 249
BsnI GGCC 4 cut(s) 103, 266, 544, 681
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 30
Bsp143I GATC 5 cut(s) 397, 502, 589, 624, 631
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 2 cut(s) 71, 682
BspANI GGCC 4 cut(s) 103, 266, 544, 681
BspCNI CTCAG 5 cut(s) 130, 295, 304, 436, 529
BspHI TCATGA 1 cut(s) 592
BspPI GGATC 2 cut(s) 392, 597
BspQI GCTCTTC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 2 cut(s) 59, 406
BssECI CCNNGG 3 cut(s) 121, 145, 366
BssMI GATC 5 cut(s) 397, 502, 589, 624, 631
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 2 cut(s) 105, 147
Bst4CI ACNGT 3 cut(s) 47, 610, 677
Bst6I CTCTTC 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 6 cut(s) 138, 291, 303, 355, 444, 537
BstF5I GGATG 4 cut(s) 73, 313, 322, 652
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 5 cut(s) 400, 505, 592, 627, 634
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 5 cut(s) 397, 502, 589, 624, 631
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 2 cut(s) 105, 147
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 103, 145
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 4 cut(s) 103, 266, 544, 681
BtsCI GGATG 4 cut(s) 73, 313, 322, 652
BtsI GCAGTG 2 cut(s) 24, 236
BtsIMutI CAGTG 4 cut(s) 24, 192, 236, 682
Cac8I GCNNGC 1 cut(s) 243
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 606
CviAII CATG 3 cut(s) 95, 593, 665
CviQI GTAC 1 cut(s) 606
DdeI CTNAG 6 cut(s) 138, 291, 303, 355, 444, 537
DpnI GATC 5 cut(s) 399, 504, 591, 626, 633
DpnII GATC 5 cut(s) 397, 502, 589, 624, 631
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 471
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 378, 605
Eco88I CYCGRG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 103, 145
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 3 cut(s) 98, 596, 668
FaiI YATR 9 cut(s) 96, 157, 159, 213, 233, 286, 483, 594, 666
FatI CATG 3 cut(s) 94, 592, 664
Fnu4HI GCNGC 1 cut(s) 682
FokI GGATG 4 cut(s) 80, 320, 329, 659
Fsp4HI GCNGC 1 cut(s) 682
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 499
GluI GCNGC 1 cut(s) 682
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 4 cut(s) 103, 266, 544, 681
HhaI GCGC 1 cut(s) 500
Hin1II CATG 3 cut(s) 98, 596, 668
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HinfI GANTC 4 cut(s) 320, 335, 413, 533
HphI GGTGA 3 cut(s) 85, 92, 362
Hpy188I TCNGA 6 cut(s) 294, 325, 340, 397, 538, 624
Hpy188III TCNNGA 3 cut(s) 252, 455, 593
HpyAV CCTTC 2 cut(s) 402, 570
HpyCH4III ACNGT 3 cut(s) 47, 610, 677
HpyCH4V TGCA 3 cut(s) 94, 241, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 6 cut(s) 138, 291, 303, 355, 444, 537
Hsp92II CATG 3 cut(s) 98, 596, 668
HspAI GCGC 1 cut(s) 498
Kzo9I GATC 5 cut(s) 397, 502, 589, 624, 631
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 2 cut(s) 85, 661
LweI GCATC 3 cut(s) 58, 81, 637
MaeI CTAG 1 cut(s) 599
MaeIII GTNAC 3 cut(s) 188, 350, 370
MalI GATC 5 cut(s) 399, 504, 591, 626, 633
MboI GATC 5 cut(s) 397, 502, 589, 624, 631
MboII GAAGA 4 cut(s) 485, 488, 524, 665
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 1 cut(s) 397
MluCI AATT 4 cut(s) 20, 462, 484, 570
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 390, 489
Mph1103I ATGCAT 1 cut(s) 96
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 3 cut(s) 15, 450, 688
MslI CAYNNNNRTG 1 cut(s) 377
MspR9I CCNGG 2 cut(s) 105, 147
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 2 cut(s) 105, 147
MwoI GCNNNNNNNGC 1 cut(s) 100
NdeII GATC 5 cut(s) 397, 502, 589, 624, 631
NlaIII CATG 3 cut(s) 98, 596, 668
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 3 cut(s) 188, 350, 370
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 1 cut(s) 98
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 1 cut(s) 592
PceI AGGCCT 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 335, 413, 533
PkrI GCNGC 1 cut(s) 683
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 103, 145
PspGI CCWGG 2 cut(s) 103, 145
PspXI VCTCGAGB 1 cut(s) 30
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 1 cut(s) 607
RsaNI GTAC 1 cut(s) 606
RseI CAYNNNNRTG 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 3 cut(s) 15, 450, 688
SatI GCNGC 1 cut(s) 682
Sau3AI GATC 5 cut(s) 397, 502, 589, 624, 631
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 105, 147
SetI ASST 9 cut(s) 90, 211, 275, 372, 429, 450, 600, 604, 621
SfaNI GCATC 3 cut(s) 58, 81, 637
Sfr274I CTCGAG 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 30, 614
SmoI CTYRAG 2 cut(s) 30, 614
Sse9I AATT 4 cut(s) 20, 462, 484, 570
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 2 cut(s) 71, 682
SspMI CTAG 1 cut(s) 599
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 103, 145
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 3 cut(s) 47, 610, 677
TaqI TCGA 1 cut(s) 31
TasI AATT 4 cut(s) 20, 462, 484, 570
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 3 cut(s) 335, 413, 533
Tru1I TTAA 3 cut(s) 15, 450, 688
Tru9I TTAA 3 cut(s) 15, 450, 688
TscAI CASTG 4 cut(s) 31, 199, 243, 682
TseFI GTSAC 3 cut(s) 188, 350, 370
Tsp45I GTSAC 3 cut(s) 188, 350, 370
TspDTI ATGAA 2 cut(s) 69, 681
TspRI CASTG 4 cut(s) 31, 199, 243, 682
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 1 cut(s) 599
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.