Rorug01G0386400

Chloride conductance regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008009
Physical Location & Seq
Reverse (-)
49787377 .. 49789964
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0386400.1

Sequence Viewer

Length: 1500 bp
ATGGATTATGAAGAACAGGTTTCCATTCAAATTCCATCTCTCATCAAATACATATCCTCAAATGAAGTGGTTGGTTTCGATAATGTTGAGGAGCATACCGTTCTCAGTGACAAGAATCAGCCATCAGCTAGGGAAATTGAGATAGCTACTTTAGAAAGCGCAGTTGATAAAGATGAACAACCAATTCATCAAAGGACGCACTCTGTTTCTATTAGTATGCCGCCATCACCCATGGGAGTTCATTTAGAGAAAATAAAAGGAGTTTTCTTCAGTCCTGAAACAATTTTCAACACTGGAATTCCAGATTCTTCTTCTGCTGCAACTAAGAGTGGTAGTAGCCGCAAACTTCCGGAAGCAGCAAAGTTTCACTCTCAACCCTTGCCAAAAGGCTCTACATTTGAAGATCAGGCAATGCACACTGGACATTTCCCTTATCATCCAAGTATTAAAAGGCTGAGAGATAATAGGTTTGACAATTTCAAGACTTGGTCTGGGAAACTTGAAAGGCAGATATCACTACTACGGGGAAAGACACCGCGAAAAAGTGATCAACCAGAGAATGCGTATGCTTTTGGTCAAAGTACAGATCAAATTGACCCTTTACCTGCAGGACGTTACTTTGATGCATTGGAAGGGCCTGAGTTGGACACTCTTAGGGCTTCAGAGGAAATAATGCTTCCAGAAGACAAGCAGTGGCCATTTCTTCTCCGGTATCCCATTTCTTCATTTAGCATCTGCCTTGGGTGGATGTCAGGAGGGCAACGCCGGCTTTCAAAAGTAGCCAATCCTGTTAACCATCTCTCCATTGCTGGCAACTTTGTTGGGGCTCTGCTAGGTGCTTCGATGGGACTAAAAGAAGGACCAATATTCTTTTTCGCTGTTGGGATGGCTCACTATATGGTCTTATTTGTAACACTCTACCAGAGACTTCCAACAAATGAGACAGTAATCCCAAAGGAGCTCCATCCTGTGTTCTTTCTGTTTGTTGCTGCACCTAGCGTAGCTTCCATGGCTTGGGCAAGACTCCAAGGCTCCTTTGATTATGGCTCGCGGATTGGTTACTTCATTTCCATGTTCCTTTATCTCTCACTGGCAGTCCGGATCAATTTTTTCCGAGGATTCAAGTTCTCGTTGGCATGGTGGGCATACACTTTCCCAATGACTGGTGCTGCCATTACAACCATCAGGTACTCGAATGAAGTAACGACTGTAGCAACTCAAGCTTTGGCTGTCATACTCTCCATCATTGCCACGCTCACGGTCACTGCTCTCTTCATAACAACCGTCTTGCATGCCTTTGTGCTCCAAGACCTCTTCCCCAATGACATTGCAATTGCCATCAGCGACAGAAGCCCAAACAAACCACGCAAAAAGTGGTTCCATCTAATAAAGGGAACCTCTTCGGATTCGAAAGACATTGAGAATTTCTTGAAGTCAGCAACAAGCTCAGAAAACAAGGATTTGGAAATTAAAACATCCCTTGAAACTGCTAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

499

Amino Acids

55.73

Weight (kDa)

7.25

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SLAC1 PF03595 245 - 429 1.3e-29 Voltage-dependent anion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 2 cut(s) 1014, 1163
AccII CGCG 2 cut(s) 538, 1051
AccIII TCCGGA 2 cut(s) 349, 1098
AciI CCGC 4 cut(s) 221, 340, 536, 1051
AclWI GGATC 1 cut(s) 1109
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 3 cut(s) 30, 297, 1423
AcuI CTGAAG 2 cut(s) 253, 645
AfaI GTAC 2 cut(s) 583, 1190
AfiI CCNNNNNNNGG 2 cut(s) 1014, 1163
AgsI TTSAA 9 cut(s) 29, 289, 401, 481, 503, 774, 1123, 1432, 1484
AluBI AGCT 7 cut(s) 128, 146, 961, 1004, 1223, 1446, 1495
AluI AGCT 7 cut(s) 128, 146, 961, 1004, 1223, 1446, 1495
Alw21I GWGCWC 2 cut(s) 963, 1305
Alw26I GTCTC 2 cut(s) 919, 935
AlwI GGATC 1 cut(s) 1109
Aor13HI TCCGGA 2 cut(s) 349, 1098
AoxI GGCC 2 cut(s) 635, 695
ApeKI GCWGC 4 cut(s) 317, 356, 989, 1169
ApoI RAATTY 3 cut(s) 30, 297, 1423
Asp700I GAANNNNTTC 1 cut(s) 1399
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 635, 860
AsuHPI GGTGA 1 cut(s) 219
AsuII TTCGAA 1 cut(s) 1409
AvaII GGWCC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 697
BanII GRGCYC 2 cut(s) 829, 963
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 2 cut(s) 963, 1305
BbvI GCAGC 4 cut(s) 304, 368, 976, 1156
BciVI GTATCC 1 cut(s) 723
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 919, 935
BfaI CTAG 4 cut(s) 129, 833, 996, 1498
BfmI CTRYAG 2 cut(s) 606, 1209
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 1 cut(s) 723
BisI GCNGC 6 cut(s) 221, 318, 340, 357, 990, 1170
BlpI GCTNAGC 1 cut(s) 1491
BlsI GCNGC 6 cut(s) 222, 319, 341, 358, 991, 1171
Bme18I GGWCC 1 cut(s) 860
BmgT120I GGNCC 2 cut(s) 635, 860
BmiI GGNNCC 3 cut(s) 1033, 1379, 1396
BmsI GCATC 2 cut(s) 613, 741
BpiI GAAGAC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 1491
Bpu14I TTCGAA 1 cut(s) 1409
BpuEI CTTGAG 1 cut(s) 1203
BsaJI CCNNGG 5 cut(s) 231, 739, 1008, 1027, 1114
BsaWI WCCGGW 3 cut(s) 349, 708, 1098
BsaXI ACNNNNNCTCC 2 cut(s) 785, 815
Bsc4I CCNNNNNNNGG 2 cut(s) 1014, 1163
Bse118I RCCGGY 1 cut(s) 765
Bse1I ACTGG 4 cut(s) 298, 424, 1095, 1168
Bse3DI GCAATG 4 cut(s) 417, 804, 1245, 1326
BseAI TCCGGA 2 cut(s) 349, 1098
BseDI CCNNGG 5 cut(s) 231, 739, 1008, 1027, 1114
BseGI GGATG 5 cut(s) 436, 753, 891, 964, 1475
BseLI CCNNNNNNNGG 2 cut(s) 1014, 1163
BseMI GCAATG 4 cut(s) 417, 804, 1245, 1326
BseMII CTCAG 4 cut(s) 118, 446, 630, 1461
BseNI ACTGG 4 cut(s) 298, 424, 1095, 1168
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 4 cut(s) 304, 368, 976, 1156
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 2 cut(s) 538, 1051
BshFI GGCC 2 cut(s) 637, 697
BsiHKAI GWGCWC 2 cut(s) 963, 1305
BsiSI CCGG 4 cut(s) 350, 709, 766, 1099
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 2 cut(s) 1014, 1163
BsmAI GTCTC 2 cut(s) 919, 935
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 2 cut(s) 637, 697
Bsp119I TTCGAA 1 cut(s) 1409
Bsp1286I GDGCHC 3 cut(s) 829, 963, 1305
Bsp13I TCCGGA 2 cut(s) 349, 1098
Bsp143I GATC 4 cut(s) 403, 547, 586, 1101
Bsp1720I GCTNAGC 1 cut(s) 1491
Bsp19I CCATGG 2 cut(s) 231, 1008
BspACI CCGC 4 cut(s) 221, 340, 536, 1051
BspANI GGCC 2 cut(s) 637, 697
BspCNI CTCAG 4 cut(s) 117, 447, 631, 1460
BspEI TCCGGA 2 cut(s) 349, 1098
BspFNI CGCG 2 cut(s) 538, 1051
BspLI GGNNCC 3 cut(s) 1033, 1379, 1396
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 1 cut(s) 1109
BspT104I TTCGAA 1 cut(s) 1409
BsrDI GCAATG 4 cut(s) 417, 804, 1245, 1326
BsrFI RCCGGY 1 cut(s) 765
BsrI ACTGG 4 cut(s) 298, 424, 1095, 1168
BssAI RCCGGY 1 cut(s) 765
BssECI CCNNGG 5 cut(s) 231, 739, 1008, 1027, 1114
BssMI GATC 4 cut(s) 403, 547, 586, 1101
BssT1I CCWWGG 4 cut(s) 231, 739, 1008, 1027
Bst4CI ACNGT 5 cut(s) 100, 946, 1210, 1261, 1285
Bst6I CTCTTC 3 cut(s) 1277, 1319, 1405
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 4 cut(s) 767, 811, 1049, 1293
BstDEI CTNAG 7 cut(s) 104, 324, 455, 639, 653, 1447, 1491
BstDSI CCRYGG 2 cut(s) 231, 1008
BstF5I GGATG 5 cut(s) 436, 753, 891, 964, 1475
BstFNI CGCG 2 cut(s) 538, 1051
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 4 cut(s) 406, 550, 589, 1104
BstMAI GTCTC 2 cut(s) 919, 935
BstMBI GATC 4 cut(s) 403, 547, 586, 1101
BstMWI GCNNNNNNNGC 5 cut(s) 766, 1010, 1142, 1220, 1350
BstNSI RCATGY 1 cut(s) 1295
BstSFI CTRYAG 2 cut(s) 606, 1209
BstUI CGCG 2 cut(s) 538, 1051
BstV1I GCAGC 4 cut(s) 304, 368, 976, 1156
BstV2I GAAGAC 1 cut(s) 690
BsuI GTATCC 1 cut(s) 723
BsuRI GGCC 2 cut(s) 637, 697
BtgI CCRYGG 2 cut(s) 231, 1008
BtsCI GGATG 5 cut(s) 436, 753, 891, 964, 1475
BtsI GCAGTG 2 cut(s) 698, 1263
BtsIMutI CAGTG 6 cut(s) 112, 291, 417, 698, 1088, 1263
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 4 cut(s) 767, 811, 1049, 1293
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 765
Cfr13I GGNCC 2 cut(s) 635, 860
CseI GACGC 1 cut(s) 205
Csp6I GTAC 2 cut(s) 582, 1189
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 5 cut(s) 232, 1009, 1072, 1137, 1292
CviQI GTAC 2 cut(s) 582, 1189
DdeI CTNAG 7 cut(s) 104, 324, 455, 639, 653, 1447, 1491
DpnI GATC 4 cut(s) 405, 549, 588, 1103
DpnII GATC 4 cut(s) 403, 547, 586, 1101
EaeI YGGCCR 1 cut(s) 695
Eam1104I CTCTTC 3 cut(s) 1277, 1319, 1405
EarI CTCTTC 3 cut(s) 1277, 1319, 1405
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 4 cut(s) 231, 739, 1008, 1027
Eco24I GRGCYC 2 cut(s) 829, 963
Eco32I GATATC 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 860
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 2 cut(s) 253, 645
EcoICRI GAGCTC 1 cut(s) 961
EcoO109I RGGNCCY 1 cut(s) 635
EcoRI GAATTC 1 cut(s) 297
EcoRV GATATC 1 cut(s) 513
EcoT14I CCWWGG 4 cut(s) 231, 739, 1008, 1027
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 2 cut(s) 829, 963
ErhI CCWWGG 4 cut(s) 231, 739, 1008, 1027
FaeI CATG 5 cut(s) 235, 1012, 1075, 1140, 1295
FaqI GGGAC 1 cut(s) 861
FatI CATG 5 cut(s) 231, 1008, 1071, 1136, 1291
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 6 cut(s) 221, 318, 340, 357, 990, 1170
FokI GGATG 5 cut(s) 423, 760, 898, 951, 1462
FriOI GRGCYC 2 cut(s) 829, 963
Fsp4HI GCNGC 6 cut(s) 221, 318, 340, 357, 990, 1170
FspBI CTAG 4 cut(s) 129, 833, 996, 1498
GlaI GCGC 1 cut(s) 160
GluI GCNGC 6 cut(s) 221, 318, 340, 357, 990, 1170
HaeIII GGCC 2 cut(s) 637, 697
HapII CCGG 4 cut(s) 350, 709, 766, 1099
HgaI GACGC 1 cut(s) 205
HhaI GCGC 1 cut(s) 161
Hin1II CATG 5 cut(s) 235, 1012, 1075, 1140, 1295
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 5 cut(s) 115, 305, 1023, 1119, 1406
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 4 cut(s) 350, 709, 766, 1099
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 4 cut(s) 664, 1115, 1405, 1450
Hpy188III TCNNGA 8 cut(s) 275, 302, 350, 481, 680, 753, 1099, 1429
Hpy8I GTNNAC 1 cut(s) 793
HpyAV CCTTC 2 cut(s) 626, 851
HpyCH4III ACNGT 5 cut(s) 100, 946, 1210, 1261, 1285
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 7 cut(s) 320, 415, 608, 626, 992, 1291, 1331
HpyF10VI GCNNNNNNNGC 5 cut(s) 766, 1010, 1142, 1220, 1350
HpyF3I CTNAG 7 cut(s) 104, 324, 455, 639, 653, 1447, 1491
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 5 cut(s) 235, 1012, 1075, 1140, 1295
HspAI GCGC 1 cut(s) 159
Kpn2I TCCGGA 2 cut(s) 349, 1098
KroI GCCGGC 1 cut(s) 765
KroNI GCCGGC 1 cut(s) 767
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 4 cut(s) 403, 547, 586, 1101
LmnI GCTCC 5 cut(s) 91, 958, 966, 1037, 1308
Lsp1109I GCAGC 4 cut(s) 304, 368, 976, 1156
LweI GCATC 2 cut(s) 613, 741
MaeI CTAG 4 cut(s) 129, 833, 996, 1498
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 6 cut(s) 107, 614, 910, 1058, 1201, 1261
MalI GATC 4 cut(s) 405, 549, 588, 1103
MboI GATC 4 cut(s) 403, 547, 586, 1101
MfeI CAATTG 1 cut(s) 1332
MhlI GDGCHC 3 cut(s) 829, 963, 1305
MlsI TGGCCA 1 cut(s) 697
MluNI TGGCCA 1 cut(s) 697
MlyI GAGTC 1 cut(s) 1017
MmeI TCCRAC 2 cut(s) 624, 956
MnlI CCTC 7 cut(s) 67, 82, 658, 749, 1109, 1322, 1408
Mox20I TGGCCA 1 cut(s) 697
Mph1103I ATGCAT 1 cut(s) 628
MroI TCCGGA 2 cut(s) 349, 1098
MroNI GCCGGC 1 cut(s) 765
MroXI GAANNNNTTC 1 cut(s) 1399
MscI TGGCCA 1 cut(s) 697
MseI TTAA 3 cut(s) 447, 792, 1470
MslI CAYNNNNRTG 1 cut(s) 1070
Msp20I TGGCCA 1 cut(s) 697
MspI CCGG 4 cut(s) 350, 709, 766, 1099
MunI CAATTG 1 cut(s) 1332
Mva1269I GAATGC 1 cut(s) 565
MvnI CGCG 2 cut(s) 538, 1051
MwoI GCNNNNNNNGC 5 cut(s) 766, 1010, 1142, 1220, 1350
NaeI GCCGGC 1 cut(s) 767
NcoI CCATGG 2 cut(s) 231, 1008
NdeII GATC 4 cut(s) 403, 547, 586, 1101
NgoMIV GCCGGC 1 cut(s) 765
NlaIII CATG 5 cut(s) 235, 1012, 1075, 1140, 1295
NlaIV GGNNCC 3 cut(s) 1033, 1379, 1396
NmuCI GTSAC 2 cut(s) 107, 1261
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 1 cut(s) 1295
NspV TTCGAA 1 cut(s) 1409
PaeI GCATGC 1 cut(s) 1295
PctI GAATGC 1 cut(s) 565
PdiI GCCGGC 1 cut(s) 767
PdmI GAANNNNTTC 1 cut(s) 1399
PfeI GAWTC 4 cut(s) 115, 305, 1119, 1406
PflFI GACNNNGTC 1 cut(s) 487
PflMI CCANNNNNTGG 2 cut(s) 1014, 1163
PkrI GCNGC 6 cut(s) 222, 319, 341, 358, 991, 1171
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 963
PspN4I GGNNCC 3 cut(s) 1033, 1379, 1396
PspPI GGNCC 2 cut(s) 635, 860
PstI CTGCAG 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 487
RsaI GTAC 2 cut(s) 583, 1190
RsaNI GTAC 2 cut(s) 582, 1189
RseI CAYNNNNRTG 1 cut(s) 1070
SacI GAGCTC 1 cut(s) 963
SaqAI TTAA 3 cut(s) 447, 792, 1470
SatI GCNGC 6 cut(s) 221, 318, 340, 357, 990, 1170
Sau3AI GATC 4 cut(s) 403, 547, 586, 1101
Sau96I GGNCC 2 cut(s) 635, 860
SbfI CCTGCAGG 1 cut(s) 610
SchI GAGTC 1 cut(s) 1017
SdaI CCTGCAGG 1 cut(s) 610
SduI GDGCHC 3 cut(s) 829, 963, 1305
SfaNI GCATC 2 cut(s) 613, 741
SfcI CTRYAG 2 cut(s) 606, 1209
SfuI TTCGAA 1 cut(s) 1409
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 1 cut(s) 1218
SmoI CTYRAG 1 cut(s) 1218
SphI GCATGC 1 cut(s) 1295
Sse8387I CCTGCAGG 1 cut(s) 610
SsiI CCGC 4 cut(s) 221, 340, 536, 1051
SspI AATATT 1 cut(s) 867
SspMI CTAG 4 cut(s) 129, 833, 996, 1498
SstI GAGCTC 1 cut(s) 963
StyI CCWWGG 4 cut(s) 231, 739, 1008, 1027
TaaI ACNGT 5 cut(s) 100, 946, 1210, 1261, 1285
TaiI ACGT 1 cut(s) 616
TaqI TCGA 4 cut(s) 78, 842, 1193, 1409
TatI WGTACW 1 cut(s) 581
TauI GCSGC 2 cut(s) 223, 342
TfiI GAWTC 4 cut(s) 115, 305, 1119, 1406
Tru1I TTAA 3 cut(s) 447, 792, 1470
Tru9I TTAA 3 cut(s) 447, 792, 1470
TscAI CASTG 6 cut(s) 112, 298, 424, 698, 1095, 1270
TseFI GTSAC 2 cut(s) 107, 1261
TseI GCWGC 4 cut(s) 317, 356, 989, 1169
Tsp45I GTSAC 2 cut(s) 107, 1261
TspDTI ATGAA 9 cut(s) 24, 78, 176, 189, 230, 714, 1054, 1212, 1264
TspRI CASTG 6 cut(s) 112, 298, 424, 698, 1095, 1270
Tth111I GACNNNGTC 1 cut(s) 487
Van91I CCANNNNNTGG 2 cut(s) 1014, 1163
VpaK11BI GGWCC 1 cut(s) 860
XapI RAATTY 3 cut(s) 30, 297, 1423
XceI RCATGY 1 cut(s) 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1371
XmnI GAANNNNTTC 1 cut(s) 1399
XspI CTAG 4 cut(s) 129, 833, 996, 1498
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.