pycom07g22990

Chloride conductance regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008009
Physical Location & Seq
Reverse (-)
24099752 .. 24101383
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22990.1

Sequence Viewer

Length: 693 bp
ATGGTGGCAGGGCTTAAAGAATTCACTGCAAGAGTTGAAAGAAACGGTGTTTATGAACCGGTACTGGATGCGGCGAACGGTGAGGAGCTGATGCACGTTCAGCCTGGTGTTTCTATTGTCCTCGGTAATCGACCCCCTGAGTCCCCTGGCACCCTCTACATCTCCACCAAGCAAGTGGTTTGGTTGAGTGACGTTGATAGGGCGAAGGGTTATGCTGTGGACTTCTTGTCCATATCACTGCACGCTGTATCCAGAGATCCGGAGGCCTACACCTCTCCTTGCATTTACACTCAGATTGAAACTGAGGATGACGAGGATGAGTCTGACGGTTCAGATTCAGAATCCAATGGTGTCTTAGATTTATCCAAGATCACAGAAATGAGACTTGTTCCTTCAGATCCTAACCAATTGGATTCTCTGTTTGAGGTATTTTGCGAGTGTGCTGAGCTTAATCCTGAACCAATTGAAGAAGGGGAAGAAGAGCGTAACTGGATTTTTAGTGCCGATCAGTTGGAAGACGAGGCAGTTGGGGACGATTCTGAGTGGCCTGTTCCTCAAAATCTTTCAAACCCAATTGGTCAATCTAACGGGGATCATGACCTAGCTCGTACTGTGCTTGAGCTTCAGATAAACGATCAGCGCTTTGAGGATGCTGAAGAAATGGAGCATGAGAGCGACAGCGACCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Voldacs

Protein Analysis

231

Amino Acids

25.66

Weight (kDa)

4.05

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voldacs PF03517 50 - 175 2e-26 Regulator of volume decrease after cellular swelling
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 2 cut(s) 71, 685
AclWI GGATC 3 cut(s) 251, 392, 600
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 3 cut(s) 378, 608, 675
AfaI GTAC 2 cut(s) 63, 610
AfeI AGCGCT 1 cut(s) 641
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 4 cut(s) 38, 299, 467, 567
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 103, 145
AluBI AGCT 4 cut(s) 88, 448, 605, 622
AluI AGCT 4 cut(s) 88, 448, 605, 622
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 3 cut(s) 251, 392, 600
Aor13HI TCCGGA 1 cut(s) 259
Aor51HI AGCGCT 1 cut(s) 641
AoxI GGCC 2 cut(s) 264, 545
ApoI RAATTY 1 cut(s) 20
AsiGI ACCGGT 1 cut(s) 58
AspLEI GCGC 1 cut(s) 642
AsuHPI GGTGA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 522
BciT130I CCWGG 2 cut(s) 105, 147
BciVI GTATCC 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 1 cut(s) 602
BfoI RGCGCY 1 cut(s) 643
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 1 cut(s) 72
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 105, 147
BmeRI GACNNNNNGTC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 105, 147
BmsI GCATC 3 cut(s) 58, 81, 640
BpiI GAAGAC 1 cut(s) 522
Bpu1102I GCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 638
BsaJI CCNNGG 2 cut(s) 121, 145
BsaWI WCCGGW 2 cut(s) 58, 259
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 69, 494
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 2 cut(s) 105, 147
BseDI CCNNGG 2 cut(s) 121, 145
BseGI GGATG 4 cut(s) 73, 313, 322, 655
BseMII CTCAG 5 cut(s) 129, 294, 305, 435, 531
BseNI ACTGG 2 cut(s) 69, 494
BseRI GAGGAG 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 685
BshFI GGCC 2 cut(s) 266, 547
BshNI GGYRCC 1 cut(s) 149
BshTI ACCGGT 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 685
BsiSI CCGG 2 cut(s) 59, 260
BslFI GGGAC 2 cut(s) 127, 545
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 127, 545
BsnI GGCC 2 cut(s) 266, 547
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 6 cut(s) 256, 369, 397, 505, 592, 634
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 2 cut(s) 71, 685
BspANI GGCC 2 cut(s) 266, 547
BspCNI CTCAG 5 cut(s) 130, 295, 304, 436, 532
BspEI TCCGGA 1 cut(s) 259
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 3 cut(s) 251, 392, 600
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 2 cut(s) 69, 494
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 121, 145
BssMI GATC 6 cut(s) 256, 369, 397, 505, 592, 634
Bst2UI CCWGG 2 cut(s) 105, 147
Bst4CI ACNGT 4 cut(s) 47, 80, 329, 613
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 6 cut(s) 138, 291, 303, 355, 444, 540
BstF5I GGATG 4 cut(s) 73, 313, 322, 655
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 1 cut(s) 642
BstKTI GATC 6 cut(s) 259, 372, 400, 508, 595, 637
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 256, 369, 397, 505, 592, 634
BstMCI CGRYCG 1 cut(s) 685
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 2 cut(s) 105, 147
BstSCI CCNGG 2 cut(s) 103, 145
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 2 cut(s) 256, 397
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 2 cut(s) 256, 397
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 266, 547
BtsCI GGATG 4 cut(s) 73, 313, 322, 655
BtsI GCAGTG 2 cut(s) 24, 236
BtsIMutI CAGTG 2 cut(s) 24, 236
Cac8I GCNNGC 1 cut(s) 243
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 642
Cfr10I RCCGGY 1 cut(s) 58
Csp6I GTAC 2 cut(s) 62, 609
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 2 cut(s) 596, 668
CviJI RGCY 8 cut(s) 13, 88, 103, 266, 448, 547, 605, 622
CviKI_1 RGCY 8 cut(s) 13, 88, 103, 266, 448, 547, 605, 622
CviQI GTAC 2 cut(s) 62, 609
DdeI CTNAG 6 cut(s) 138, 291, 303, 355, 444, 540
DpnI GATC 6 cut(s) 258, 371, 399, 507, 594, 636
DpnII GATC 6 cut(s) 256, 369, 397, 505, 592, 634
DriI GACNNNNNGTC 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 474
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 474
Eco147I AGGCCT 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 641
Eco57I CTGAAG 3 cut(s) 378, 608, 675
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 103, 145
FaeI CATG 2 cut(s) 599, 671
FaiI YATR 5 cut(s) 54, 213, 233, 597, 669
FaqI GGGAC 2 cut(s) 127, 545
FatI CATG 2 cut(s) 595, 667
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 4 cut(s) 80, 320, 329, 662
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 1 cut(s) 602
GlaI GCGC 1 cut(s) 641
GluI GCNGC 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 2 cut(s) 266, 547
HapII CCGG 2 cut(s) 59, 260
HhaI GCGC 1 cut(s) 642
Hin1II CATG 2 cut(s) 599, 671
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HinfI GANTC 6 cut(s) 140, 320, 335, 341, 413, 536
HpaII CCGG 2 cut(s) 59, 260
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 7 cut(s) 294, 325, 334, 340, 397, 541, 627
Hpy188III TCNNGA 4 cut(s) 252, 260, 455, 596
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 199, 402, 464
HpyCH4III ACNGT 4 cut(s) 47, 80, 329, 613
HpyCH4IV ACGT 2 cut(s) 96, 192
HpyCH4V TGCA 4 cut(s) 29, 94, 241, 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 6 cut(s) 138, 291, 303, 355, 444, 540
HpySE526I ACGT 2 cut(s) 96, 192
Hsp92II CATG 2 cut(s) 599, 671
HspAI GCGC 1 cut(s) 640
Kpn2I TCCGGA 1 cut(s) 259
Kzo9I GATC 6 cut(s) 256, 369, 397, 505, 592, 634
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 85, 664
LweI GCATC 3 cut(s) 58, 81, 640
MaeI CTAG 1 cut(s) 602
MaeII ACGT 2 cut(s) 96, 192
MaeIII GTNAC 2 cut(s) 188, 485
MalI GATC 6 cut(s) 258, 371, 399, 507, 594, 636
MboI GATC 6 cut(s) 256, 369, 397, 505, 592, 634
MboII GAAGA 5 cut(s) 479, 488, 491, 527, 668
MfeI CAATTG 3 cut(s) 407, 462, 573
MflI RGATCY 2 cut(s) 256, 397
MluCI AATT 4 cut(s) 20, 407, 462, 573
MlyI GAGTC 2 cut(s) 149, 329
MmeI TCCRAC 1 cut(s) 492
MroI TCCGGA 1 cut(s) 259
MseI TTAA 3 cut(s) 15, 450, 691
MslI CAYNNNNRTG 1 cut(s) 377
MspI CCGG 2 cut(s) 59, 260
MspR9I CCNGG 2 cut(s) 105, 147
MunI CAATTG 3 cut(s) 407, 462, 573
MvaI CCWGG 2 cut(s) 105, 147
MwoI GCNNNNNNNGC 1 cut(s) 100
NdeII GATC 6 cut(s) 256, 369, 397, 505, 592, 634
NlaIII CATG 2 cut(s) 599, 671
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 188
PagI TCATGA 1 cut(s) 595
PceI AGGCCT 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 335, 341, 413, 536
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 1 cut(s) 73
PleI GAGTC 2 cut(s) 148, 328
PpsI GAGTC 2 cut(s) 148, 328
Psp6I CCWGG 2 cut(s) 103, 145
PspGI CCWGG 2 cut(s) 103, 145
PspN4I GGNNCC 1 cut(s) 151
PsuI RGATCY 2 cut(s) 256, 397
RsaI GTAC 2 cut(s) 63, 610
RsaNI GTAC 2 cut(s) 62, 609
RseI CAYNNNNRTG 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 3 cut(s) 15, 450, 691
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 6 cut(s) 256, 369, 397, 505, 592, 634
SchI GAGTC 2 cut(s) 149, 329
ScrFI CCNGG 2 cut(s) 105, 147
SetI ASST 9 cut(s) 90, 99, 195, 275, 429, 450, 603, 607, 624
SfaNI GCATC 3 cut(s) 58, 81, 640
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 4 cut(s) 20, 407, 462, 573
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 2 cut(s) 71, 685
SspMI CTAG 1 cut(s) 602
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 103, 145
TaaI ACNGT 4 cut(s) 47, 80, 329, 613
TaiI ACGT 2 cut(s) 99, 195
TaqI TCGA 1 cut(s) 130
TasI AATT 4 cut(s) 20, 407, 462, 573
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 4 cut(s) 335, 341, 413, 536
Tru1I TTAA 3 cut(s) 15, 450, 691
Tru9I TTAA 3 cut(s) 15, 450, 691
TscAI CASTG 2 cut(s) 31, 243
TseFI GTSAC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 2 cut(s) 31, 243
XapI RAATTY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.