AT5G62865

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011856
Physical Location & Seq
Forward (+)
25233881 .. 25234884
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62865.1

Sequence Viewer

Length: 504 bp
ATGGAACTTTCGCAGTCGGACCCAACCCGAGATCCTGATACCCGGTACGATCAACGTTGCTGTTGTTTCCCCAGCTTCCGCCGATCAAGAAGCTCCACCGCCGTTGGTTACTCCTCCTGGGGACGAATTCGAACCGTCGATGACAGTAATCACAGCGGCGACCACGGCGACGAGCCACGTTGGTGGATCCGAGCATCCTTGAAGATCCGAGAGTGGTCAGAAATCGTTGCTGGTCCACGGTGGAAGACTTTCATTCGTCGATTCAATCGCGATCCACGACGCGGCCGCGATTGGGACGCAAGCGAGAAGTTTCAATACGATCCTTTGAGTTACTCTTTGAACTTCGATGACGACGACGAGGAGGATGAATACGTCGGACTAGGTGGATTACGAAGCTTCTCGACCCGATTCGCTTCTGTCCCGGTTTACTCAGGTAAAGCTCCGGCGATTTCGCCGACGTCGTTGTCTGCGTTGACGCCGCGTAATGAGATCATTGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

19.31

Weight (kDa)

5.76

Isoelectric Point (pI)

60.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48020 AT5G62865
fragaria_vesca FvH4_5g05080
malus_domestica MD00G1171700.v1.1 MD14G1183900.v1.1
prunus_persica Prupe.5G176600_v2.0.a1
pyrus_communis pycom06g15780 pycom14g15300
rosa_chinensis RchiOBHm_Chr7g0193921
rosa_laevigata RLG00000004231 RLG00000004233
rosa_multiflora Rmu_sc0009542.1_g000001 Rmu_sc0037969.1_g000001
rosa_roxburghii Rroxscaffold_3G00261690
rosa_rugosa Rorug07G0012800
rosa_samantha Rh7AG139800 Rh7BG140200 Rh7DG141200
rosa_wichuraiana Rw7G011930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 463
AatII GACGTC 1 cut(s) 461
AccII CGCG 4 cut(s) 270, 282, 288, 481
AciI CCGC 6 cut(s) 79, 99, 156, 282, 286, 479
AclI AACGTT 1 cut(s) 55
AclWI GGATC 6 cut(s) 26, 181, 194, 199, 266, 314
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 458, 476
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 281, 292
AgsI TTSAA 5 cut(s) 202, 265, 314, 340, 497
AjnI CCWGG 1 cut(s) 116
AleI CACNNNNGTG 1 cut(s) 181
AluBI AGCT 4 cut(s) 75, 93, 396, 440
AluI AGCT 4 cut(s) 75, 93, 396, 440
AlwI GGATC 6 cut(s) 26, 181, 194, 199, 266, 314
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 19, 233
AsuC2I CCSGG 2 cut(s) 43, 422
AsuII TTCGAA 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 2 cut(s) 19, 233
BamHI GGATCC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BbsI GAAGAC 1 cut(s) 251
BceAI ACGGC 2 cut(s) 86, 181
BciT130I CCWGG 1 cut(s) 118
BcnI CCSGG 2 cut(s) 43, 422
BfaI CTAG 1 cut(s) 380
BisI GCNGC 4 cut(s) 157, 283, 286, 479
BlsI GCNGC 4 cut(s) 158, 284, 287, 480
Bme1390I CCNGG 3 cut(s) 43, 118, 422
Bme18I GGWCC 2 cut(s) 19, 233
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 19, 233
BmiI GGNNCC 2 cut(s) 21, 188
BmrFI CCNGG 3 cut(s) 43, 118, 422
BmsI GCATC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 130
BpuMI CCSGG 2 cut(s) 43, 422
BsaHI GRCGYC 2 cut(s) 458, 476
BsaJI CCNNGG 3 cut(s) 117, 163, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 292
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 117, 163, 236
BseGI GGATG 2 cut(s) 194, 370
BseLI CCNNNNNNNGG 2 cut(s) 281, 292
BseMII CTCAG 1 cut(s) 444
BseRI GAGGAG 2 cut(s) 103, 374
BseX3I CGGCCG 1 cut(s) 283
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 4 cut(s) 270, 282, 288, 481
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 3 cut(s) 43, 422, 443
BslFI GGGAC 3 cut(s) 135, 308, 404
BslI CCNNNNNNNGG 2 cut(s) 281, 292
BsmFI GGGAC 3 cut(s) 135, 308, 404
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 130
Bsp143I GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
Bsp68I TCGCGA 1 cut(s) 270
BspACI CCGC 6 cut(s) 79, 99, 156, 282, 286, 479
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 443
BspFNI CGCG 4 cut(s) 270, 282, 288, 481
BspLI GGNNCC 2 cut(s) 21, 188
BspPI GGATC 6 cut(s) 26, 181, 194, 199, 266, 314
BspT104I TTCGAA 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 117, 163, 236
BssMI GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
BssNI GRCGYC 2 cut(s) 458, 476
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 3 cut(s) 136, 146, 240
BstACI GRCGYC 2 cut(s) 458, 476
BstBI TTCGAA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 430
BstDSI CCRYGG 2 cut(s) 163, 236
BstF5I GGATG 2 cut(s) 194, 370
BstFNI CGCG 4 cut(s) 270, 282, 288, 481
BstKTI GATC 8 cut(s) 34, 52, 86, 189, 207, 274, 322, 492
BstMBI GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 41, 116, 420
BstUI CGCG 4 cut(s) 270, 282, 288, 481
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 3 cut(s) 31, 186, 204
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 3 cut(s) 31, 186, 204
BstZI CGGCCG 1 cut(s) 283
BsuRI GGCC 1 cut(s) 285
BtgI CCRYGG 2 cut(s) 163, 236
BtsCI GGATG 2 cut(s) 194, 370
BtuMI TCGCGA 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 301
CciNI GCGGCCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 19, 233
CseI GACGC 3 cut(s) 288, 305, 484
Csp6I GTAC 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviJI RGCY 6 cut(s) 75, 93, 175, 285, 396, 440
CviKI_1 RGCY 6 cut(s) 75, 93, 175, 285, 396, 440
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 1 cut(s) 430
DpnI GATC 8 cut(s) 33, 51, 85, 188, 206, 273, 321, 491
DpnII GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
DrdI GACNNNNNNGTC 1 cut(s) 463
DseDI GACNNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 283
EagI CGGCCG 1 cut(s) 283
EciI GGCGGA 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 19, 233
Eco52I CGGCCG 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 116
FaqI GGGAC 3 cut(s) 135, 308, 404
Fnu4HI GCNGC 4 cut(s) 157, 283, 286, 479
FokI GGATG 2 cut(s) 181, 377
Fsp4HI GCNGC 4 cut(s) 157, 283, 286, 479
FspBI CTAG 1 cut(s) 380
GluI GCNGC 4 cut(s) 157, 283, 286, 479
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 285
HapII CCGG 3 cut(s) 43, 422, 443
HgaI GACGC 3 cut(s) 288, 305, 484
Hin1I GRCGYC 2 cut(s) 458, 476
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 2 cut(s) 261, 408
HpaII CCGG 3 cut(s) 43, 422, 443
Hpy166II GTNNAC 3 cut(s) 236, 427, 474
Hpy188I TCNGA 5 cut(s) 19, 191, 209, 220, 377
Hpy188III TCNNGA 4 cut(s) 35, 87, 269, 400
Hpy8I GTNNAC 3 cut(s) 236, 427, 474
Hpy99I CGWCG 9 cut(s) 140, 173, 261, 282, 356, 359, 377, 460, 463
HpyCH4III ACNGT 3 cut(s) 136, 146, 240
HpyCH4IV ACGT 4 cut(s) 55, 178, 372, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 430
HpySE526I ACGT 4 cut(s) 55, 178, 372, 458
Hsp92I GRCGYC 2 cut(s) 458, 476
Kzo9I GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
LmnI GCTCC 2 cut(s) 98, 445
LpnPI CCDG 9 cut(s) 48, 56, 85, 103, 130, 216, 417, 435, 456
LweI GCATC 1 cut(s) 203
MaeI CTAG 1 cut(s) 380
MaeII ACGT 4 cut(s) 55, 178, 372, 458
MaeIII GTNAC 2 cut(s) 107, 329
MalI GATC 8 cut(s) 33, 51, 85, 188, 206, 273, 321, 491
MboI GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
MboII GAAGA 2 cut(s) 214, 256
MflI RGATCY 3 cut(s) 31, 186, 204
MluCI AATT 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 3 cut(s) 124, 352, 355
MroXI GAANNNNTTC 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 181
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 3 cut(s) 43, 422, 443
MspR9I CCNGG 3 cut(s) 43, 118, 422
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 4 cut(s) 270, 282, 288, 481
MwoI GCNNNNNNNGC 1 cut(s) 165
NciI CCSGG 2 cut(s) 43, 422
NdeII GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
NlaIV GGNNCC 2 cut(s) 21, 188
NotI GCGGCCGC 1 cut(s) 283
NruI TCGCGA 1 cut(s) 270
NspV TTCGAA 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 181
PcsI WCGNNNNNNNCGW 4 cut(s) 274, 351, 458, 467
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 2 cut(s) 261, 408
PkrI GCNGC 4 cut(s) 158, 284, 287, 480
Psp1406I AACGTT 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 21, 188
PspPI GGNCC 2 cut(s) 19, 233
PsuI RGATCY 3 cut(s) 31, 186, 204
RruI TCGCGA 1 cut(s) 270
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 181
SatI GCNGC 4 cut(s) 157, 283, 286, 479
Sau3AI GATC 8 cut(s) 31, 49, 83, 186, 204, 271, 319, 489
Sau96I GGNCC 2 cut(s) 19, 233
ScrFI CCNGG 3 cut(s) 43, 118, 422
SfaNI GCATC 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 130
SinI GGWCC 2 cut(s) 19, 233
SmiMI CAYNNNNRTG 1 cut(s) 181
Sse9I AATT 1 cut(s) 126
SsiI CCGC 6 cut(s) 79, 99, 156, 282, 286, 479
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 41, 116, 420
TaaI ACNGT 3 cut(s) 136, 146, 240
TaiI ACGT 4 cut(s) 58, 181, 375, 461
TaqI TCGA 5 cut(s) 130, 138, 259, 345, 401
TasI AATT 1 cut(s) 126
TauI GCSGC 4 cut(s) 159, 285, 288, 481
TfiI GAWTC 2 cut(s) 261, 408
TspDTI ATGAA 2 cut(s) 241, 381
VpaK11BI GGWCC 2 cut(s) 19, 233
XapI RAATTY 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 380
ZraI GACGTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.