MD14G1183900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011856
Physical Location & Seq
Forward (+)
27634377 .. 27634805
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1183900.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGAAGACATTGACTTGGAGTCCATAGGCCATGGAGGATCAAGGTCGCGCTGCTGCTTCTGTATTCCGTCGTCATGGTCGCGGATGCGGACCTCCCAAAACGATGACCGGTGGTGGTCCCGAGGCATCAAGGCCCTCTACAAGCTTCGAGAGTGGTCCGAGATCGTCGCCGGCCCCAAGTGGAAGACTTTCATCCGCCGGTTCAACCGAAGCAGAAGCGGAGGCGGTTCCTCCGGCGGCGGCGGCAGGCACGGGAAATTCCAATACGATCCCTTGAGTTACGCCCTGAACTTCGACGAGGGACCGGTCGACGAAGATGACGAAGCCGGCGGGTTCCGCGATTTCTCGGCCCGGTTCGCCTCGATCCCGCAACCGGCGAAGTCCGAGGGGCCGCCGGAATCGGGTAAAGAAGTGTCGGTGTACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.85

Weight (kDa)

7.71

Isoelectric Point (pI)

58.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48020 AT5G62865
fragaria_vesca FvH4_5g05080
malus_domestica MD00G1171700.v1.1 MD14G1183900.v1.1
prunus_persica Prupe.5G176600_v2.0.a1
pyrus_communis pycom06g15780 pycom14g15300
rosa_chinensis RchiOBHm_Chr7g0193921
rosa_laevigata RLG00000004231 RLG00000004233
rosa_multiflora Rmu_sc0009542.1_g000001 Rmu_sc0037969.1_g000001
rosa_roxburghii Rroxscaffold_3G00261690
rosa_rugosa Rorug07G0012800
rosa_samantha Rh7AG139800 Rh7BG140200 Rh7DG141200
rosa_wichuraiana Rw7G011930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AccII CGCG 3 cut(s) 49, 82, 339
AclWI GGATC 3 cut(s) 46, 263, 358
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 2 cut(s) 373, 401
AgeI ACCGGT 2 cut(s) 108, 304
AgsI TTSAA 1 cut(s) 205
AhdI GACNNNNNGTC 1 cut(s) 19
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
AlwI GGATC 3 cut(s) 46, 263, 358
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 5 cut(s) 28, 132, 172, 348, 389
ApeKI GCWGC 2 cut(s) 51, 54
ApoI RAATTY 1 cut(s) 257
AsiGI ACCGGT 2 cut(s) 108, 304
Asp700I GAANNNNTTC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 8 cut(s) 90, 117, 133, 156, 173, 302, 349, 389
AsuC2I CCSGG 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 4 cut(s) 90, 117, 156, 302
BbsI GAAGAC 2 cut(s) 12, 191
BbvI GCAGC 2 cut(s) 38, 41
BcnI CCSGG 1 cut(s) 352
BisI GCNGC 6 cut(s) 52, 55, 238, 241, 244, 392
BlsI GCNGC 6 cut(s) 53, 56, 239, 242, 245, 393
Bme1390I CCNGG 1 cut(s) 352
Bme18I GGWCC 4 cut(s) 90, 117, 156, 302
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 8 cut(s) 90, 117, 133, 156, 173, 302, 349, 389
BmiI GGNNCC 6 cut(s) 119, 175, 229, 303, 335, 390
BmrFI CCNGG 1 cut(s) 352
BmsI GCATC 2 cut(s) 75, 135
BpiI GAAGAC 2 cut(s) 12, 191
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 31, 121, 384
BsaWI WCCGGW 2 cut(s) 108, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 401
Bse118I RCCGGY 6 cut(s) 108, 170, 198, 304, 326, 373
BseDI CCNNGG 3 cut(s) 31, 121, 384
BseGI GGATG 2 cut(s) 90, 192
BseLI CCNNNNNNNGG 2 cut(s) 373, 401
BseXI GCAGC 2 cut(s) 38, 41
Bsh1236I CGCG 3 cut(s) 49, 82, 339
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 5 cut(s) 30, 134, 174, 350, 391
BshTI ACCGGT 2 cut(s) 108, 304
BsiEI CGRYCG 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 9 cut(s) 109, 171, 199, 234, 305, 327, 352, 374, 395
BslFI GGGAC 2 cut(s) 103, 315
BslI CCNNNNNNNGG 2 cut(s) 373, 401
BsmFI GGGAC 2 cut(s) 103, 315
BsnI GGCC 5 cut(s) 30, 134, 174, 350, 391
BsoBI CYCGRG 1 cut(s) 120
Bsp143I GATC 4 cut(s) 38, 162, 268, 363
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 5 cut(s) 30, 134, 174, 350, 391
BspFNI CGCG 3 cut(s) 49, 82, 339
BspLI GGNNCC 6 cut(s) 119, 175, 229, 303, 335, 390
BspPI GGATC 3 cut(s) 46, 263, 358
BsrFI RCCGGY 6 cut(s) 108, 170, 198, 304, 326, 373
BssAI RCCGGY 6 cut(s) 108, 170, 198, 304, 326, 373
BssECI CCNNGG 3 cut(s) 31, 121, 384
BssMI GATC 4 cut(s) 38, 162, 268, 363
BssT1I CCWWGG 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 172, 248, 328
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 90, 192
BstFNI CGCG 3 cut(s) 49, 82, 339
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 41, 165, 271, 366
BstMBI GATC 4 cut(s) 38, 162, 268, 363
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 3 cut(s) 243, 336, 356
BstSCI CCNGG 1 cut(s) 350
BstUI CGCG 3 cut(s) 49, 82, 339
BstV1I GCAGC 2 cut(s) 38, 41
BstV2I GAAGAC 2 cut(s) 12, 191
BsuRI GGCC 5 cut(s) 30, 134, 174, 350, 391
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 2 cut(s) 90, 192
Cac8I GCNNGC 3 cut(s) 172, 248, 328
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 6 cut(s) 108, 170, 198, 304, 326, 373
Cfr13I GGNCC 8 cut(s) 90, 117, 133, 156, 173, 302, 349, 389
Csp6I GTAC 1 cut(s) 421
CspAI ACCGGT 2 cut(s) 108, 304
CviAII CATG 2 cut(s) 32, 75
CviJI RGCY 7 cut(s) 30, 134, 145, 174, 326, 350, 391
CviKI_1 RGCY 7 cut(s) 30, 134, 145, 174, 326, 350, 391
CviQI GTAC 1 cut(s) 421
DpnI GATC 4 cut(s) 40, 164, 270, 365
DpnII GATC 4 cut(s) 38, 162, 268, 363
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 31
Eco47I GGWCC 4 cut(s) 90, 117, 156, 302
Eco88I CYCGRG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 2 cut(s) 35, 78
FaiI YATR 3 cut(s) 26, 33, 76
FaqI GGGAC 2 cut(s) 103, 315
FatI CATG 2 cut(s) 31, 74
FauI CCCGC 2 cut(s) 323, 375
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 52, 55, 238, 241, 244, 392
FokI GGATG 2 cut(s) 97, 179
Fsp4HI GCNGC 6 cut(s) 52, 55, 238, 241, 244, 392
GlaI GCGC 1 cut(s) 50
GluI GCNGC 6 cut(s) 52, 55, 238, 241, 244, 392
HaeIII GGCC 5 cut(s) 30, 134, 174, 350, 391
HapII CCGG 9 cut(s) 109, 171, 199, 234, 305, 327, 352, 374, 395
HhaI GCGC 1 cut(s) 51
Hin1II CATG 2 cut(s) 35, 78
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 2 cut(s) 20, 398
HpaII CCGG 9 cut(s) 109, 171, 199, 234, 305, 327, 352, 374, 395
Hpy166II GTNNAC 2 cut(s) 310, 421
Hpy188I TCNGA 2 cut(s) 160, 385
Hpy188III TCNNGA 2 cut(s) 120, 149
Hpy8I GTNNAC 2 cut(s) 310, 421
Hpy99I CGWCG 4 cut(s) 73, 170, 299, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 336, 356
Hsp92II CATG 2 cut(s) 35, 78
HspAI GCGC 1 cut(s) 49
KroI GCCGGC 2 cut(s) 170, 326
KroNI GCCGGC 2 cut(s) 172, 328
Kzo9I GATC 4 cut(s) 38, 162, 268, 363
Lsp1109I GCAGC 2 cut(s) 38, 41
LweI GCATC 2 cut(s) 75, 135
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 40, 164, 270, 365
MboI GATC 4 cut(s) 38, 162, 268, 363
MboII GAAGA 3 cut(s) 17, 196, 326
MluCI AATT 1 cut(s) 257
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 9 cut(s) 29, 103, 116, 146, 215, 241, 292, 370, 379
MroNI GCCGGC 2 cut(s) 170, 326
MroXI GAANNNNTTC 1 cut(s) 188
MspI CCGG 9 cut(s) 109, 171, 199, 234, 305, 327, 352, 374, 395
MspR9I CCNGG 1 cut(s) 352
MvnI CGCG 3 cut(s) 49, 82, 339
MwoI GCNNNNNNNGC 3 cut(s) 243, 336, 356
NaeI GCCGGC 2 cut(s) 172, 328
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 31
NdeII GATC 4 cut(s) 38, 162, 268, 363
NgoMIV GCCGGC 2 cut(s) 170, 326
NlaIII CATG 2 cut(s) 35, 78
NlaIV GGNNCC 6 cut(s) 119, 175, 229, 303, 335, 390
NmeAIII GCCGAG 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 2 cut(s) 172, 328
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 398
PinAI ACCGGT 2 cut(s) 108, 304
PkrI GCNGC 6 cut(s) 53, 56, 239, 242, 245, 393
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 6 cut(s) 119, 175, 229, 303, 335, 390
PspPI GGNCC 8 cut(s) 90, 117, 133, 156, 173, 302, 349, 389
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SalI GTCGAC 1 cut(s) 308
SatI GCNGC 6 cut(s) 52, 55, 238, 241, 244, 392
Sau3AI GATC 4 cut(s) 38, 162, 268, 363
Sau96I GGNCC 8 cut(s) 90, 117, 133, 156, 173, 302, 349, 389
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 352
SetI ASST 3 cut(s) 47, 95, 147
SfaNI GCATC 2 cut(s) 75, 135
SinI GGWCC 4 cut(s) 90, 117, 156, 302
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 350
StyI CCWWGG 1 cut(s) 31
TaqI TCGA 4 cut(s) 148, 294, 309, 362
TasI AATT 1 cut(s) 257
TauI GCSGC 4 cut(s) 240, 243, 246, 394
TfiI GAWTC 1 cut(s) 398
TseI GCWGC 2 cut(s) 51, 54
TspDTI ATGAA 1 cut(s) 181
TspGWI ACGGA 1 cut(s) 57
VpaK11BI GGWCC 4 cut(s) 90, 117, 156, 302
XapI RAATTY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.