MD00G1171700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011856
Physical Location & Seq
Reverse (-)
39476641 .. 39477221
581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1171700.v1.1.491

Sequence Viewer

Length: 540 bp
ATGCTTCCGTTACAAACTTCCGTTTCTCAACGGCACCTGTATAAACACCCCGTTCCGCAGAGAGCCACGAAATTTCTCAATTGGGGGTTTTCCAAAGCCCCTCTCTCTCTCTTCATGGAGGACATTGACATGGAGTCAATAGGCCATGGAGGAACAAGTTCGCGCTGCTGCTTCTGTTTTCCGTCGTCGTGGTCGCGGATGCGGACCTCCCAAAACGATGACCGGTGGTGGTCCCGAGGCATCAGGGCCTTGTACAAGCTTCGAGAGTGGTCCGAGATCGTCGCCGGCCCCAGGTGGAAGACTTTCATCCGCCGATTCAACCGAAGCAGAAGCATAGGCCGTGGCGCCGGCGCCAGCAATCACGGGAAATTCCAATACGATCCCTTGAGTTACGCTCTGAACTTCGACGAGGGCCCCGTCGACGAAGACGACGAAGTCGTGGGGTTCCGGAATTTCTCGGTCCGGTACGCCTCGTTTCCTCAACCGGCAAAGTCCGGGGGTTTGACGGAATCGGGCACAGACGTGGCGGTGTACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

20.4

Weight (kDa)

9.43

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48020 AT5G62865
fragaria_vesca FvH4_5g05080
malus_domestica MD00G1171700.v1.1 MD14G1183900.v1.1
prunus_persica Prupe.5G176600_v2.0.a1
pyrus_communis pycom06g15780 pycom14g15300
rosa_chinensis RchiOBHm_Chr7g0193921
rosa_laevigata RLG00000004231 RLG00000004233
rosa_multiflora Rmu_sc0009542.1_g000001 Rmu_sc0037969.1_g000001
rosa_roxburghii Rroxscaffold_3G00261690
rosa_rugosa Rorug07G0012800
rosa_samantha Rh7AG139800 Rh7BG140200 Rh7DG141200
rosa_wichuraiana Rw7G011930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AccB1I GGYRCC 3 cut(s) 33, 344, 350
AccI GTMKAC 1 cut(s) 420
AccII CGCG 2 cut(s) 163, 196
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 5 cut(s) 56, 196, 202, 310, 527
AclWI GGATC 1 cut(s) 374
AcsI RAATTY 3 cut(s) 71, 368, 451
AcyI GRCGYC 2 cut(s) 345, 351
AfaI GTAC 3 cut(s) 254, 467, 533
AfiI CCNNNNNNNGG 1 cut(s) 291
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 133
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 290
AleI CACNNNNGTG 1 cut(s) 521
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
AlwI GGATC 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 234
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 5 cut(s) 142, 246, 286, 337, 412
ApaI GGGCCC 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 165, 168
ApoI RAATTY 3 cut(s) 71, 368, 451
AsiGI ACCGGT 1 cut(s) 222
Asp700I GAANNNNTTC 2 cut(s) 157, 302
AspLEI GCGC 3 cut(s) 165, 347, 353
AspS9I GGNCC 8 cut(s) 204, 231, 246, 270, 287, 412, 413, 460
AsuC2I CCSGG 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 234
AvaII GGWCC 4 cut(s) 204, 231, 270, 460
BaeGI GKGCMC 2 cut(s) 416, 518
BanI GGYRCC 3 cut(s) 33, 344, 350
BanII GRGCYC 1 cut(s) 416
BbsI GAAGAC 2 cut(s) 305, 432
BbvI GCAGC 2 cut(s) 152, 155
BceAI ACGGC 2 cut(s) 47, 324
BciT130I CCWGG 1 cut(s) 292
BcnI CCSGG 1 cut(s) 496
BfoI RGCGCY 2 cut(s) 348, 354
BisI GCNGC 2 cut(s) 166, 169
BlsI GCNGC 2 cut(s) 167, 170
Bme1390I CCNGG 2 cut(s) 292, 496
Bme18I GGWCC 4 cut(s) 204, 231, 270, 460
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 234
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 8 cut(s) 204, 231, 246, 270, 287, 412, 413, 460
BmiI GGNNCC 8 cut(s) 35, 233, 289, 346, 352, 414, 415, 446
BmrFI CCNGG 2 cut(s) 292, 496
BmsI GCATC 2 cut(s) 189, 249
BpiI GAAGAC 2 cut(s) 305, 432
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 496
BsaHI GRCGYC 2 cut(s) 345, 351
BsaJI CCNNGG 5 cut(s) 145, 235, 290, 340, 495
BsaWI WCCGGW 3 cut(s) 222, 447, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 4 cut(s) 222, 284, 347, 484
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 5 cut(s) 145, 235, 290, 340, 495
BseGI GGATG 2 cut(s) 204, 306
BseLI CCNNNNNNNGG 1 cut(s) 291
BseSI GKGCMC 2 cut(s) 416, 518
BseXI GCAGC 2 cut(s) 152, 155
Bsh1236I CGCG 2 cut(s) 163, 196
BshFI GGCC 5 cut(s) 144, 248, 288, 339, 414
BshNI GGYRCC 3 cut(s) 33, 344, 350
BshTI ACCGGT 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 7 cut(s) 223, 285, 348, 448, 463, 485, 495
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 5 cut(s) 144, 248, 288, 339, 414
BsoBI CYCGRG 1 cut(s) 234
Bsp120I GGGCCC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 416, 518
Bsp13I TCCGGA 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 2 cut(s) 276, 379
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 5 cut(s) 56, 196, 202, 310, 527
BspANI GGCC 5 cut(s) 144, 248, 288, 339, 414
BspEI TCCGGA 1 cut(s) 447
BspFNI CGCG 2 cut(s) 163, 196
BspLI GGNNCC 8 cut(s) 35, 233, 289, 346, 352, 414, 415, 446
BspPI GGATC 1 cut(s) 374
BspT107I GGYRCC 3 cut(s) 33, 344, 350
BsrFI RCCGGY 4 cut(s) 222, 284, 347, 484
BsrGI TGTACA 1 cut(s) 252
BssAI RCCGGY 4 cut(s) 222, 284, 347, 484
BssECI CCNNGG 5 cut(s) 145, 235, 290, 340, 495
BssMI GATC 2 cut(s) 276, 379
BssNI GRCGYC 2 cut(s) 345, 351
BssT1I CCWWGG 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 345, 351
BstAUI TGTACA 1 cut(s) 252
BstC8I GCNNGC 3 cut(s) 286, 349, 355
BstDSI CCRYGG 2 cut(s) 145, 340
BstF5I GGATG 2 cut(s) 204, 306
BstFNI CGCG 2 cut(s) 163, 196
BstH2I RGCGCY 2 cut(s) 348, 354
BstHHI GCGC 3 cut(s) 165, 347, 353
BstKTI GATC 2 cut(s) 279, 382
BstMBI GATC 2 cut(s) 276, 379
BstNI CCWGG 1 cut(s) 292
BstSCI CCNGG 2 cut(s) 290, 494
BstSLI GKGCMC 2 cut(s) 416, 518
BstUI CGCG 2 cut(s) 163, 196
BstV1I GCAGC 2 cut(s) 152, 155
BstV2I GAAGAC 2 cut(s) 305, 432
BsuRI GGCC 5 cut(s) 144, 248, 288, 339, 414
BtgI CCRYGG 2 cut(s) 145, 340
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 2 cut(s) 204, 306
Cac8I GCNNGC 3 cut(s) 286, 349, 355
CfoI GCGC 3 cut(s) 165, 347, 353
Cfr10I RCCGGY 4 cut(s) 222, 284, 347, 484
Cfr13I GGNCC 8 cut(s) 204, 231, 246, 270, 287, 412, 413, 460
CpoI CGGWCCG 1 cut(s) 460
Csp6I GTAC 3 cut(s) 253, 466, 532
CspAI ACCGGT 1 cut(s) 222
CspI CGGWCCG 1 cut(s) 460
CviAII CATG 3 cut(s) 115, 130, 146
CviJI RGCY 8 cut(s) 65, 98, 144, 248, 259, 288, 339, 414
CviKI_1 RGCY 8 cut(s) 65, 98, 144, 248, 259, 288, 339, 414
CviQI GTAC 3 cut(s) 253, 466, 532
DinI GGCGCC 2 cut(s) 346, 352
DpnI GATC 2 cut(s) 278, 381
DpnII GATC 2 cut(s) 276, 379
DrdI GACNNNNNNGTC 1 cut(s) 434
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 116
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 416
Eco47I GGWCC 4 cut(s) 204, 231, 270, 460
Eco88I CYCGRG 1 cut(s) 234
EcoO109I RGGNCCY 3 cut(s) 246, 412, 413
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 416
EgeI GGCGCC 2 cut(s) 346, 352
EheI GGCGCC 2 cut(s) 346, 352
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 118, 133, 149
FaiI YATR 5 cut(s) 42, 116, 131, 147, 335
FaqI GGGAC 1 cut(s) 217
FatI CATG 3 cut(s) 114, 129, 145
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 166, 169
FokI GGATG 2 cut(s) 211, 293
FriOI GRGCYC 1 cut(s) 416
Fsp4HI GCNGC 2 cut(s) 166, 169
GlaI GCGC 3 cut(s) 164, 346, 352
GluI GCNGC 2 cut(s) 166, 169
HaeII RGCGCY 2 cut(s) 348, 354
HaeIII GGCC 5 cut(s) 144, 248, 288, 339, 414
HapII CCGG 7 cut(s) 223, 285, 348, 448, 463, 485, 495
HhaI GCGC 3 cut(s) 165, 347, 353
Hin1I GRCGYC 2 cut(s) 345, 351
Hin1II CATG 3 cut(s) 118, 133, 149
Hin6I GCGC 3 cut(s) 163, 345, 351
HinP1I GCGC 3 cut(s) 163, 345, 351
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 3 cut(s) 134, 315, 509
HpaII CCGG 7 cut(s) 223, 285, 348, 448, 463, 485, 495
Hpy166II GTNNAC 2 cut(s) 421, 532
Hpy188I TCNGA 2 cut(s) 274, 399
Hpy188III TCNNGA 3 cut(s) 234, 263, 448
Hpy8I GTNNAC 2 cut(s) 421, 532
Hpy99I CGWCG 7 cut(s) 187, 190, 284, 410, 422, 425, 434
HpyCH4IV ACGT 1 cut(s) 522
HpySE526I ACGT 1 cut(s) 522
Hsp92I GRCGYC 2 cut(s) 345, 351
Hsp92II CATG 3 cut(s) 118, 133, 149
HspAI GCGC 3 cut(s) 163, 345, 351
KasI GGCGCC 2 cut(s) 344, 350
Kpn2I TCCGGA 1 cut(s) 447
KroI GCCGGC 2 cut(s) 284, 347
KroNI GCCGGC 2 cut(s) 286, 349
Kzo9I GATC 2 cut(s) 276, 379
Lsp1109I GCAGC 2 cut(s) 152, 155
LweI GCATC 2 cut(s) 189, 249
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 9, 389
MalI GATC 2 cut(s) 278, 381
MboI GATC 2 cut(s) 276, 379
MboII GAAGA 3 cut(s) 103, 310, 437
MfeI CAATTG 1 cut(s) 79
MhlI GDGCHC 2 cut(s) 416, 518
MluCI AATT 4 cut(s) 71, 79, 368, 451
Mly113I GGCGCC 2 cut(s) 345, 351
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 8 cut(s) 111, 112, 143, 217, 230, 403, 481, 489
MreI CGCCGGCG 1 cut(s) 347
MroI TCCGGA 1 cut(s) 447
MroNI GCCGGC 2 cut(s) 284, 347
MroXI GAANNNNTTC 2 cut(s) 157, 302
MslI CAYNNNNRTG 2 cut(s) 128, 521
MspI CCGG 7 cut(s) 223, 285, 348, 448, 463, 485, 495
MspR9I CCNGG 2 cut(s) 292, 496
MunI CAATTG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 2 cut(s) 163, 196
NaeI GCCGGC 2 cut(s) 286, 349
NarI GGCGCC 2 cut(s) 345, 351
NciI CCSGG 1 cut(s) 496
NcoI CCATGG 1 cut(s) 145
NdeII GATC 2 cut(s) 276, 379
NgoMIV GCCGGC 2 cut(s) 284, 347
NlaIII CATG 3 cut(s) 118, 133, 149
NlaIV GGNNCC 8 cut(s) 35, 233, 289, 346, 352, 414, 415, 446
OliI CACNNNNGTG 1 cut(s) 521
PcsI WCGNNNNNNNCGW 4 cut(s) 414, 426, 429, 435
PdiI GCCGGC 2 cut(s) 286, 349
PdmI GAANNNNTTC 2 cut(s) 157, 302
PfeI GAWTC 2 cut(s) 315, 509
PflFI GACNNNGTC 1 cut(s) 434
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 2 cut(s) 167, 170
PleI GAGTC 1 cut(s) 142
PluTI GGCGCC 2 cut(s) 348, 354
PpsI GAGTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 8 cut(s) 35, 233, 289, 346, 352, 414, 415, 446
PspOMI GGGCCC 1 cut(s) 412
PspPI GGNCC 8 cut(s) 204, 231, 246, 270, 287, 412, 413, 460
PsyI GACNNNGTC 1 cut(s) 434
RsaI GTAC 3 cut(s) 254, 467, 533
RsaNI GTAC 3 cut(s) 253, 466, 532
RseI CAYNNNNRTG 2 cut(s) 128, 521
Rsr2I CGGWCCG 1 cut(s) 460
RsrII CGGWCCG 1 cut(s) 460
SalI GTCGAC 1 cut(s) 419
SatI GCNGC 2 cut(s) 166, 169
Sau3AI GATC 2 cut(s) 276, 379
Sau96I GGNCC 8 cut(s) 204, 231, 246, 270, 287, 412, 413, 460
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 292, 496
SduI GDGCHC 2 cut(s) 416, 518
SetI ASST 5 cut(s) 39, 209, 261, 296, 525
SfaNI GCATC 2 cut(s) 189, 249
SfoI GGCGCC 2 cut(s) 346, 352
SgrAI CRCCGGYG 1 cut(s) 347
SgrDI CGTCGACG 1 cut(s) 419
SinI GGWCC 4 cut(s) 204, 231, 270, 460
SmiMI CAYNNNNRTG 2 cut(s) 128, 521
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 4 cut(s) 71, 79, 368, 451
SsiI CCGC 5 cut(s) 56, 196, 202, 310, 527
SspDI GGCGCC 2 cut(s) 344, 350
StyD4I CCNGG 2 cut(s) 290, 494
StyI CCWWGG 1 cut(s) 145
TaiI ACGT 1 cut(s) 525
TaqI TCGA 3 cut(s) 262, 405, 420
TaqII GACCGA 1 cut(s) 448
TasI AATT 4 cut(s) 71, 79, 368, 451
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 315, 509
TseI GCWGC 2 cut(s) 165, 168
TspDTI ATGAA 2 cut(s) 103, 295
TspGWI ACGGA 3 cut(s) 10, 171, 521
Tth111I GACNNNGTC 1 cut(s) 434
VpaK11BI GGWCC 4 cut(s) 204, 231, 270, 460
XapI RAATTY 3 cut(s) 71, 368, 451
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 2 cut(s) 157, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.