AT5G63080

Cupin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008370
Physical Location & Seq
Reverse (-)
25299933 .. 25302476
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63080.1

Sequence Viewer

Length: 1389 bp
ATGGGAATACAGATTATAGGTCAAATTGAGAGAATCAATGGTAAAGAGCTGAGTTACGGCGATTTTGCAGAGAGATACTTAGCTAAGAACCAGCCAGTGGTAATATCCGATCTCACTGAAGATTGGCGAGCTCGGGAAGATTGGGTCAGTGAGAATGGAAACCCTAATCTCCACGTATTTGCCACTCACTTTGGAAAATCCAGAGTTCAGGTTGCAGATTGTGATACGAGAGAATTTACAGATCAGAAAAGATTAGAAATGTCTGTTACTGAATTTGTGGAGCAATGGACTAATAAGGATTCTATTGAGGAATCTGTTTTGTATCTGAAAGATTGGCACTTTGTGAAGGAGTATCCAGACTATACAGCTTACCAAACACCGCCGTTGTTTTCTGATGATTGGCTTAACGTGTATCTAGACAATTACCAAATGCATGAGGATAGAGATAGTTTCCAGAAGTATGATCAAATAAGCTGCTCTGATTATCGGTTTGTTTATATGGGTGGGAAAGGATCTTGGACACCTCTACATGCTGATGTATTTAGATCTTATAGTTGGTCAGCTAATGTCTGTGGTAAAAAAAGATGGCTTTTCCTTCCTCCTCCTCAAAGTCATCTTGTTTATGACAGGTACATGAAAAATTGCGTTTATGACATTTTTGAAGAGGTGAATGAAACTAAATTCCCTGGTTTTAAGAAGACCACCTGGCTTGAGTGCATTCAAGAACCCGGTGAAATTATCTTTGTTCCCAGTGGATGGCATCATCAAGTATATAACTTGGAAGACACAATATCTATTAACCACAATTGGCTCAACGCATACAACCTATCTTGGGTGTGGGATCTACTGTGGAAGGACTACAAGGACACTGAAGAGTCAATAGAAGACATAAGAGACATATGTGATGATTTTGAAGCTATCTGCCAACGTAATCTTGCTGCCAACACAGGAATGAATTTAAATGATTTCTTCCTCTTCATGTCACGGTTCTCTTTGGGGAACATGGTTCTACTGCAATCTTACTCTGACAAACACAAAAACCTGAATTCGTGTTCATTAGCAATGGCCCAAAACCTGTTAATGAATCTCTCAACTATACTGAAAGTCATGATGAAGATGATATCTGCAGGCGGTGTAACTGCAGAGGAAGTGTATCTGGACTTGCGAGAAACACTGGAGGATCCACAGTTTCTCAGATTTGTCAGAGACATGGGAAGAACTTATGCAAGGATTCACATGGAGGAAGAGGATCAGTTTCTTTCTTCGAAAGAATTATTACAAAAGCTCAGTGGTCTTGCAGGTCCAAATATGCAAATATGTTCTCCAAAGGATCTTGTTGAAATGATAAACCATCACAATACTTTTTCCTCCCAAATCTATTTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8 JmjC

Protein Analysis

462

Amino Acids

54.36

Weight (kDa)

4.91

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_8 PF13621 22 - 272 8.6e-27 Cupin-like domain
JmjC PF02373 165 - 270 5.5e-16 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1289
AccB7I CCANNNNNTGG 2 cut(s) 97, 756
AciI CCGC 2 cut(s) 380, 1131
AclWI GGATC 6 cut(s) 520, 849, 1175, 1188, 1257, 1338
AcsI RAATTY 5 cut(s) 233, 272, 680, 955, 1045
AcuI CTGAAG 2 cut(s) 138, 891
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 1 cut(s) 632
AfiI CCNNNNNNNGG 3 cut(s) 97, 756, 832
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 4 cut(s) 662, 722, 914, 1340
AjnI CCWGG 2 cut(s) 685, 704
AluBI AGCT 8 cut(s) 49, 83, 131, 368, 474, 563, 917, 1285
AluI AGCT 8 cut(s) 49, 83, 131, 368, 474, 563, 917, 1285
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 2 cut(s) 888, 1200
AlwI GGATC 6 cut(s) 520, 849, 1175, 1188, 1257, 1338
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 1065
ApeKI GCWGC 2 cut(s) 474, 938
ApoI RAATTY 5 cut(s) 233, 272, 680, 955, 1045
AspS9I GGNCC 2 cut(s) 1066, 1301
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 2 cut(s) 679, 743
AsuII TTCGAA 1 cut(s) 1265
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 1301
BamHI GGATCC 1 cut(s) 1180
BanII GRGCYC 1 cut(s) 133
BbsI GAAGAC 3 cut(s) 704, 789, 891
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 461, 925
BccI CCATC 3 cut(s) 579, 750, 1359
BceAI ACGGC 2 cut(s) 73, 367
BciT130I CCWGG 2 cut(s) 687, 706
BciVI GTATCC 1 cut(s) 363
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 888, 1200
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 2 cut(s) 1125, 1140
BfuAI ACCTGC 1 cut(s) 1289
BfuI GTATCC 1 cut(s) 363
BglII AGATCT 1 cut(s) 545
BisI GCNGC 2 cut(s) 475, 939
BlsI GCNGC 2 cut(s) 476, 940
Bme1390I CCNGG 3 cut(s) 687, 706, 729
Bme18I GGWCC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 1066, 1301
BmiI GGNNCC 1 cut(s) 1182
BmrFI CCNGG 3 cut(s) 687, 706, 729
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 1 cut(s) 769
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 3 cut(s) 704, 789, 891
BpmI CTGGAG 1 cut(s) 1196
Bpu14I TTCGAA 1 cut(s) 1265
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 729
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 685
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 756, 832
Bse1I ACTGG 3 cut(s) 95, 750, 1179
Bse3DI GCAATG 2 cut(s) 290, 1068
BseBI CCWGG 2 cut(s) 687, 706
BseDI CCNNGG 1 cut(s) 685
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 3 cut(s) 97, 756, 832
BseMI GCAATG 2 cut(s) 290, 1068
BseMII CTCAG 3 cut(s) 41, 1207, 1300
BseNI ACTGG 3 cut(s) 95, 750, 1179
BseRI GAGGAG 2 cut(s) 591, 594
BseXI GCAGC 2 cut(s) 461, 925
BshFI GGCC 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 729
BslI CCNNNNNNNGG 3 cut(s) 97, 756, 832
BsmAI GTCTC 2 cut(s) 888, 1200
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 1 cut(s) 1067
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 1265
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
BspACI CCGC 2 cut(s) 380, 1131
BspANI GGCC 1 cut(s) 1067
BspCNI CTCAG 3 cut(s) 42, 1206, 1299
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 1 cut(s) 1182
BspMAI CTGCAG 2 cut(s) 1129, 1144
BspMI ACCTGC 1 cut(s) 1289
BspPI GGATC 6 cut(s) 520, 849, 1175, 1188, 1257, 1338
BspT104I TTCGAA 1 cut(s) 1265
BsrDI GCAATG 2 cut(s) 290, 1068
BsrI ACTGG 3 cut(s) 95, 750, 1179
BssECI CCNNGG 1 cut(s) 685
BssMI GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
Bst2UI CCWGG 2 cut(s) 687, 706
Bst4CI ACNGT 3 cut(s) 849, 987, 1188
Bst6I CTCTTC 4 cut(s) 657, 867, 980, 1239
BstBAI YACGTR 1 cut(s) 175
BstBI TTCGAA 1 cut(s) 1265
BstC8I GCNNGC 2 cut(s) 129, 1129
BstDEI CTNAG 5 cut(s) 50, 79, 84, 1193, 1286
BstF5I GGATG 1 cut(s) 761
BstKTI GATC 9 cut(s) 112, 244, 466, 515, 548, 844, 1183, 1252, 1333
BstMAI GTCTC 2 cut(s) 888, 1200
BstMBI GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
BstNI CCWGG 2 cut(s) 687, 706
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 3 cut(s) 685, 704, 727
BstSFI CTRYAG 2 cut(s) 1125, 1140
BstV1I GCAGC 2 cut(s) 461, 925
BstV2I GAAGAC 3 cut(s) 704, 789, 891
BstX2I RGATCY 5 cut(s) 512, 545, 841, 1180, 1330
BstYI RGATCY 5 cut(s) 512, 545, 841, 1180, 1330
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 1 cut(s) 1067
BtsCI GGATG 1 cut(s) 761
BtsIMutI CAGTG 7 cut(s) 102, 114, 154, 757, 867, 1172, 1294
BveI ACCTGC 1 cut(s) 1289
Cac8I GCNNGC 2 cut(s) 129, 1129
CciI TCATGA 1 cut(s) 1107
Cfr13I GGNCC 2 cut(s) 1066, 1301
Csp6I GTAC 1 cut(s) 631
CspCI CAANNNNNGTGG 4 cut(s) 172, 207, 691, 726
CviAII CATG 8 cut(s) 434, 530, 634, 979, 1003, 1108, 1210, 1237
CviQI GTAC 1 cut(s) 631
DdeI CTNAG 5 cut(s) 50, 79, 84, 1193, 1286
DpnI GATC 9 cut(s) 111, 243, 465, 514, 547, 843, 1182, 1251, 1332
DpnII GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
DraI TTTAAA 1 cut(s) 960
DraIII CACNNNGTG 1 cut(s) 343
Eam1104I CTCTTC 4 cut(s) 657, 867, 980, 1239
EarI CTCTTC 4 cut(s) 657, 867, 980, 1239
Ecl136II GAGCTC 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 1122
Eco47I GGWCC 1 cut(s) 1301
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 138, 891
Eco88I CYCGRG 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 685, 704
EcoRV GATATC 1 cut(s) 1122
EcoT22I ATGCAT 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 133
FaeI CATG 8 cut(s) 437, 533, 637, 982, 1006, 1111, 1213, 1240
FatI CATG 8 cut(s) 433, 529, 633, 978, 1002, 1107, 1209, 1236
FauNDI CATATG 1 cut(s) 899
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 475, 939
FokI GGATG 1 cut(s) 768
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 475, 939
FspBI CTAG 1 cut(s) 416
GluI GCNGC 2 cut(s) 475, 939
GsuI CTGGAG 1 cut(s) 1196
HaeIII GGCC 1 cut(s) 1067
HapII CCGG 1 cut(s) 729
Hin1II CATG 8 cut(s) 437, 533, 637, 982, 1006, 1111, 1213, 1240
HinfI GANTC 6 cut(s) 33, 299, 311, 875, 1084, 1231
HpaII CCGG 1 cut(s) 729
HphI GGTGA 2 cut(s) 679, 743
Hpy188I TCNGA 8 cut(s) 109, 246, 327, 394, 481, 1027, 1196, 1205
Hpy188III TCNNGA 8 cut(s) 134, 201, 356, 416, 454, 722, 1108, 1157
HpyAV CCTTC 3 cut(s) 340, 605, 847
HpyCH4III ACNGT 3 cut(s) 849, 987, 1188
HpyCH4IV ACGT 3 cut(s) 174, 408, 928
HpyF3I CTNAG 5 cut(s) 50, 79, 84, 1193, 1286
HpySE526I ACGT 3 cut(s) 174, 408, 928
Hsp92II CATG 8 cut(s) 437, 533, 637, 982, 1006, 1111, 1213, 1240
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 461, 925
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 416
MaeII ACGT 3 cut(s) 174, 408, 928
MaeIII GTNAC 4 cut(s) 53, 265, 981, 1135
MalI GATC 9 cut(s) 111, 243, 465, 514, 547, 843, 1182, 1251, 1332
MboI GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
MfeI CAATTG 1 cut(s) 805
MflI RGATCY 5 cut(s) 512, 545, 841, 1180, 1330
MhlI GDGCHC 1 cut(s) 133
MlyI GAGTC 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 435
MseI TTAA 5 cut(s) 405, 693, 798, 959, 1079
MslI CAYNNNNRTG 2 cut(s) 534, 950
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 3 cut(s) 687, 706, 729
MunI CAATTG 1 cut(s) 805
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 2 cut(s) 687, 706
NciI CCSGG 1 cut(s) 729
NdeI CATATG 1 cut(s) 899
NdeII GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
NlaIII CATG 8 cut(s) 437, 533, 637, 982, 1006, 1111, 1213, 1240
NlaIV GGNNCC 1 cut(s) 1182
NmuCI GTSAC 1 cut(s) 981
NsiI ATGCAT 1 cut(s) 435
NspI RCATGY 1 cut(s) 533
NspV TTCGAA 1 cut(s) 1265
PagI TCATGA 1 cut(s) 1107
PctI GAATGC 1 cut(s) 717
PfeI GAWTC 5 cut(s) 33, 299, 311, 1084, 1231
PflMI CCANNNNNTGG 2 cut(s) 97, 756
PkrI GCNGC 2 cut(s) 476, 940
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Ppu21I YACGTR 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 685, 704
PspGI CCWGG 2 cut(s) 685, 704
PspN4I GGNNCC 1 cut(s) 1182
PspPI GGNCC 2 cut(s) 1066, 1301
PstI CTGCAG 2 cut(s) 1129, 1144
PsuI RGATCY 5 cut(s) 512, 545, 841, 1180, 1330
RsaI GTAC 1 cut(s) 632
RsaNI GTAC 1 cut(s) 631
RseI CAYNNNNRTG 2 cut(s) 534, 950
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 5 cut(s) 405, 693, 798, 959, 1079
SatI GCNGC 2 cut(s) 475, 939
Sau3AI GATC 9 cut(s) 109, 241, 463, 512, 545, 841, 1180, 1249, 1330
Sau96I GGNCC 2 cut(s) 1066, 1301
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 3 cut(s) 687, 706, 729
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 2 cut(s) 1125, 1140
SfuI TTCGAA 1 cut(s) 1265
SinI GGWCC 1 cut(s) 1301
SmiI ATTTAAAT 1 cut(s) 960
SmiMI CAYNNNNRTG 2 cut(s) 534, 950
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SsiI CCGC 2 cut(s) 380, 1131
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 3 cut(s) 685, 704, 727
SwaI ATTTAAAT 1 cut(s) 960
TaaI ACNGT 3 cut(s) 849, 987, 1188
TaiI ACGT 3 cut(s) 177, 411, 931
TaqI TCGA 1 cut(s) 1265
TfiI GAWTC 5 cut(s) 33, 299, 311, 1084, 1231
Tru1I TTAA 5 cut(s) 405, 693, 798, 959, 1079
Tru9I TTAA 5 cut(s) 405, 693, 798, 959, 1079
TscAI CASTG 7 cut(s) 102, 121, 154, 757, 874, 1179, 1294
TseFI GTSAC 1 cut(s) 981
TseI GCWGC 2 cut(s) 474, 938
Tsp45I GTSAC 1 cut(s) 981
TspDTI ATGAA 7 cut(s) 650, 687, 967, 968, 1044, 1097, 1127
TspRI CASTG 7 cut(s) 102, 121, 154, 757, 874, 1179, 1294
Van91I CCANNNNNTGG 2 cut(s) 97, 756
VpaK11BI GGWCC 1 cut(s) 1301
XapI RAATTY 5 cut(s) 233, 272, 680, 955, 1045
XbaI TCTAGA 1 cut(s) 415
XceI RCATGY 1 cut(s) 533
XspI CTAG 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.