AT5G63140

Purple acid phosphatase 29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007460
Physical Location & Seq
Forward (+)
25328119 .. 25329882
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63140.1

Sequence Viewer

Length: 1170 bp
ATGGCGGACAATCGAAGAAGAAGAAGCCTATTCGACTTCCTACTTTTCTCCGTGTTTCTAGGACTCGCATGTCTCTGTTTATCTCCGATTCCGGCAACAGCTCAACGTCGGAAACTCAGGTTCAGTGTTAACGGCGAGTTCAAGATACTACAAGTGGCGGATATGCATTTTGCTAACGGCGCTAAGACGCAGTGTCAAAATGTTCTTCCTAGCCAGAGAGCTCATTGCTCAGACCTCAACACCACCATCTTCATGTCGCGAGTCATCGCCGCCGAGAAGCCTGATCTTATCGTCTTCACCGGTGATAACATATTTGGGTTTGATGTAAAAGACGCTTTGAAATCCATAAACGCTGCGTTTGCTCCAGCGATTGCGTCTAAAATCCCATGGGTTGCTATTTTGGGAAACCATGACCAAGAATCTACATTTACAAGACAACAAGTTATGAATCACATTGTGAAGCTTCCCAACACTTTGTCTCAAGTGAATCCTCCTGAAGCTGCTCATTACATTGATGGTTTTGGTAATTACAATCTCCAAATCCATGGAGCTGCTGATTCGAAACTACAGAACAAATCTGTTCTTAATCTTTATTTTCTCGATAGTGGAGATTATTCTAGTGTTCCTTACATGGAAGGTTATGATTGGATCAAAACTTCTCAGCAGTTTTGGTTCGACCGCACTTCAAAACGTCTCAAGAGGGAGTACAACGCAAAGCCTAATCCTCAAGAAGGTATAGCTCCAGGACTAGCTTACTTCCACATTCCATTACCGGAGTTTTTGAGTTTTGACTCAAAGAACGCTACAAAAGGAGTGAGACAAGAAGGTACATCCGCGGCTTCTACAAACTCGGGTTTCTTCACAACATTGATAGCTAGAGGAGATGTGAAATCTGTGTTTGTGGGTCACGACCATGTCAACGACTTCTGCGGTGAACTTAAAGGTTTGAATCTATGTTATGGTGGTGGATTTGGGTATCATGCTTATGGTAAAGCTGGATGGGAAAGAAGAGCGAGAGTTGTGGTTGTTGATTTGAATAAGAAGAGAAAAGGAAAGTGGGGAGCTGTGAAATCGATTAAGACATGGAAGAGGCTTGATGATAAGCATCTCTCTGTTATTGACTCTCAAGTTCTTTGGAACAATTCTGCAAACAAATTAGTTGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

389

Amino Acids

43.55

Weight (kDa)

9.5

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 47 - 306 4e-14 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 69
AccII CGCG 2 cut(s) 259, 836
AciI CCGC 7 cut(s) 5, 158, 270, 679, 834, 836, 930
AclWI GGATC 1 cut(s) 656
AcuI CTGAAG 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 2 cut(s) 707, 829
AfiI CCNNNNNNNGG 1 cut(s) 731
AgeI ACCGGT 1 cut(s) 299
AgsI TTSAA 5 cut(s) 142, 340, 687, 949, 1036
AhdI GACNNNNNGTC 1 cut(s) 192
AjnI CCWGG 1 cut(s) 742
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 4 cut(s) 77, 483, 698, 811
AlwI GGATC 1 cut(s) 656
Ama87I CYCGRG 1 cut(s) 850
ApeKI GCWGC 3 cut(s) 353, 500, 551
AsiGI ACCGGT 1 cut(s) 299
AspLEI GCGC 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 289, 314, 944
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 850
BanII GRGCYC 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 340, 487, 538
BccI CCATC 3 cut(s) 254, 509, 993
BceAI ACGGC 2 cut(s) 148, 193
BciT130I CCWGG 1 cut(s) 744
BcoDI GTCTC 4 cut(s) 77, 483, 698, 811
BfaI CTAG 5 cut(s) 59, 210, 618, 749, 876
BfmI CTRYAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 5 cut(s) 270, 354, 501, 552, 837
BlsI GCNGC 5 cut(s) 271, 355, 502, 553, 838
Bme1390I CCNGG 1 cut(s) 744
BmeRI GACNNNNNGTC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 850
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 1 cut(s) 1114
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 2 cut(s) 348, 726
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 4 cut(s) 465, 680, 711, 1110
Bsa29I ATCGAT 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 1104
BsaJI CCNNGG 3 cut(s) 386, 544, 834
BsaWI WCCGGW 2 cut(s) 299, 772
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse118I RCCGGY 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 1 cut(s) 744
BseCI ATCGAT 1 cut(s) 1073
BseDI CCNNGG 3 cut(s) 386, 544, 834
BseGI GGATG 2 cut(s) 830, 1004
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 3 cut(s) 130, 243, 674
BseRI GAGGAG 1 cut(s) 894
BseXI GCAGC 3 cut(s) 340, 487, 538
Bsh1236I CGCG 2 cut(s) 259, 836
Bsh1285I CGRYCG 1 cut(s) 679
BshTI ACCGGT 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 1073
BsiEI CGRYCG 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 3 cut(s) 92, 300, 773
BslI CCNNNNNNNGG 1 cut(s) 731
BsmAI GTCTC 4 cut(s) 77, 483, 698, 811
BsmBI CGTCTC 1 cut(s) 698
BsoBI CYCGRG 1 cut(s) 850
Bsp119I TTCGAA 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 2 cut(s) 283, 648
Bsp19I CCATGG 2 cut(s) 386, 544
Bsp68I TCGCGA 1 cut(s) 259
BspACI CCGC 7 cut(s) 5, 158, 270, 679, 834, 836, 930
BspCNI CTCAG 3 cut(s) 129, 242, 673
BspDI ATCGAT 1 cut(s) 1073
BspFNI CGCG 2 cut(s) 259, 836
BspPI GGATC 1 cut(s) 656
BspQI GCTCTTC 1 cut(s) 1003
BspT104I TTCGAA 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 386, 544, 834
BssMI GATC 2 cut(s) 283, 648
BssT1I CCWWGG 2 cut(s) 386, 544
Bst2UI CCWGG 1 cut(s) 744
Bst6I CTCTTC 3 cut(s) 1003, 1037, 1082
BstBI TTCGAA 1 cut(s) 560
BstDEI CTNAG 4 cut(s) 116, 183, 229, 660
BstDSI CCRYGG 3 cut(s) 386, 544, 834
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 2 cut(s) 830, 1004
BstFNI CGCG 2 cut(s) 259, 836
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 286, 651
BstMAI GTCTC 4 cut(s) 77, 483, 698, 811
BstMBI GATC 2 cut(s) 283, 648
BstMCI CGRYCG 1 cut(s) 679
BstMWI GCNNNNNNNGC 2 cut(s) 179, 359
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 566
BstUI CGCG 2 cut(s) 259, 836
BstV1I GCAGC 3 cut(s) 340, 487, 538
BstV2I GAAGAC 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 545
Bsu15I ATCGAT 1 cut(s) 1073
BsuTUI ATCGAT 1 cut(s) 1073
BtgI CCRYGG 3 cut(s) 386, 544, 834
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 830, 1004
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 130, 197
BtuMI TCGCGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 299
Cfr42I CCGCGG 1 cut(s) 837
ClaI ATCGAT 1 cut(s) 1073
CseI GACGC 3 cut(s) 196, 341, 363
Csp6I GTAC 2 cut(s) 706, 828
CspAI ACCGGT 1 cut(s) 299
CviAII CATG 9 cut(s) 69, 253, 387, 410, 545, 631, 914, 980, 1083
CviQI GTAC 2 cut(s) 706, 828
DdeI CTNAG 4 cut(s) 116, 183, 229, 660
DpnI GATC 2 cut(s) 285, 650
DpnII GATC 2 cut(s) 283, 648
DraIII CACNNNGTG 1 cut(s) 457
DrdI GACNNNNNNGTC 1 cut(s) 69
DriI GACNNNNNGTC 1 cut(s) 192
DseDI GACNNNNNNGTC 1 cut(s) 69
Eam1104I CTCTTC 3 cut(s) 1003, 1037, 1082
Eam1105I GACNNNNNGTC 1 cut(s) 192
EarI CTCTTC 3 cut(s) 1003, 1037, 1082
EciI GGCGGA 2 cut(s) 20, 173
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 2 cut(s) 386, 544
Eco24I GRGCYC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 742
EcoT14I CCWWGG 2 cut(s) 386, 544
EcoT22I ATGCAT 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 386, 544
Esp3I CGTCTC 1 cut(s) 698
FaeI CATG 9 cut(s) 72, 256, 390, 413, 548, 634, 917, 983, 1086
FatI CATG 9 cut(s) 68, 252, 386, 409, 544, 630, 913, 979, 1082
Fnu4HI GCNGC 5 cut(s) 270, 354, 501, 552, 837
FokI GGATG 2 cut(s) 817, 1011
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 5 cut(s) 270, 354, 501, 552, 837
FspBI CTAG 5 cut(s) 59, 210, 618, 749, 876
GlaI GCGC 1 cut(s) 181
GluI GCNGC 5 cut(s) 270, 354, 501, 552, 837
GsuI CTGGAG 2 cut(s) 348, 726
HaeII RGCGCY 1 cut(s) 183
HapII CCGG 3 cut(s) 92, 300, 773
HgaI GACGC 3 cut(s) 196, 341, 363
HhaI GCGC 1 cut(s) 182
Hin1II CATG 9 cut(s) 72, 256, 390, 413, 548, 634, 917, 983, 1086
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 2 cut(s) 130, 919
HindII GTYRAC 2 cut(s) 130, 919
HindIII AAGCTT 1 cut(s) 461
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 3 cut(s) 92, 300, 773
HphI GGTGA 3 cut(s) 289, 314, 944
Hpy166II GTNNAC 3 cut(s) 130, 919, 935
Hpy188I TCNGA 3 cut(s) 87, 111, 232
Hpy188III TCNNGA 7 cut(s) 142, 258, 494, 599, 697, 728, 908
Hpy8I GTNNAC 3 cut(s) 130, 919, 935
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 3 cut(s) 629, 725, 818
HpyCH4IV ACGT 2 cut(s) 106, 691
HpyCH4V TGCA 2 cut(s) 166, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 359
HpyF3I CTNAG 4 cut(s) 116, 183, 229, 660
HpySE526I ACGT 2 cut(s) 106, 691
Hsp92II CATG 9 cut(s) 72, 256, 390, 413, 548, 634, 917, 983, 1086
HspAI GCGC 1 cut(s) 180
KspAI GTTAAC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 837
Kzo9I GATC 2 cut(s) 283, 648
LguI GCTCTTC 1 cut(s) 1003
LmnI GCTCC 4 cut(s) 367, 548, 745, 1061
Lsp1109I GCAGC 3 cut(s) 340, 487, 538
LweI GCATC 1 cut(s) 1114
MaeI CTAG 5 cut(s) 59, 210, 618, 749, 876
MaeII ACGT 2 cut(s) 106, 691
MaeIII GTNAC 1 cut(s) 905
MalI GATC 2 cut(s) 285, 650
MboI GATC 2 cut(s) 283, 648
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 3 cut(s) 526, 1141, 1154
MlyI GAGTC 4 cut(s) 57, 270, 785, 1115
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 6 cut(s) 245, 501, 693, 735, 872, 1083
Mph1103I ATGCAT 1 cut(s) 168
MseI TTAA 4 cut(s) 129, 585, 939, 1077
MslI CAYNNNNRTG 3 cut(s) 251, 912, 984
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 3 cut(s) 92, 300, 773
MspR9I CCNGG 1 cut(s) 744
MvaI CCWGG 1 cut(s) 744
MvnI CGCG 2 cut(s) 259, 836
MwoI GCNNNNNNNGC 2 cut(s) 179, 359
NcoI CCATGG 2 cut(s) 386, 544
NdeII GATC 2 cut(s) 283, 648
NlaIII CATG 9 cut(s) 72, 256, 390, 413, 548, 634, 917, 983, 1086
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 905
NruI TCGCGA 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 168
NspI RCATGY 1 cut(s) 72
NspV TTCGAA 1 cut(s) 560
PciSI GCTCTTC 1 cut(s) 1003
PfeI GAWTC 6 cut(s) 88, 419, 448, 487, 557, 949
PflFI GACNNNGTC 1 cut(s) 914
PfoI TCCNGGA 1 cut(s) 742
PinAI ACCGGT 1 cut(s) 299
PkrI GCNGC 5 cut(s) 271, 355, 502, 553, 838
PleI GAGTC 4 cut(s) 57, 269, 785, 1115
PpsI GAGTC 4 cut(s) 57, 269, 785, 1115
Psp124BI GAGCTC 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 742
PspGI CCWGG 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 914
RruI TCGCGA 1 cut(s) 259
RsaI GTAC 2 cut(s) 707, 829
RsaNI GTAC 2 cut(s) 706, 828
RseI CAYNNNNRTG 3 cut(s) 251, 912, 984
SacI GAGCTC 1 cut(s) 223
SacII CCGCGG 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 1003
SaqAI TTAA 4 cut(s) 129, 585, 939, 1077
SatI GCNGC 5 cut(s) 270, 354, 501, 552, 837
Sau3AI GATC 2 cut(s) 283, 648
SchI GAGTC 4 cut(s) 57, 270, 785, 1115
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 1 cut(s) 1114
SfcI CTRYAG 1 cut(s) 566
Sfr303I CCGCGG 1 cut(s) 837
SfuI TTCGAA 1 cut(s) 560
SgrAI CRCCGGYG 1 cut(s) 299
SgrBI CCGCGG 1 cut(s) 837
SmiMI CAYNNNNRTG 3 cut(s) 251, 912, 984
SmlI CTYRAG 4 cut(s) 480, 695, 726, 1125
SmoI CTYRAG 4 cut(s) 480, 695, 726, 1125
Sse9I AATT 3 cut(s) 526, 1141, 1154
SsiI CCGC 7 cut(s) 5, 158, 270, 679, 834, 836, 930
SspMI CTAG 5 cut(s) 59, 210, 618, 749, 876
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 2 cut(s) 386, 544
TaiI ACGT 2 cut(s) 109, 694
TaqI TCGA 6 cut(s) 13, 33, 560, 600, 675, 1073
TasI AATT 3 cut(s) 526, 1141, 1154
TatI WGTACW 1 cut(s) 705
TauI GCSGC 2 cut(s) 272, 839
TfiI GAWTC 6 cut(s) 88, 419, 448, 487, 557, 949
Tru1I TTAA 4 cut(s) 129, 585, 939, 1077
Tru9I TTAA 4 cut(s) 129, 585, 939, 1077
TscAI CASTG 2 cut(s) 130, 197
TseFI GTSAC 1 cut(s) 905
TseI GCWGC 3 cut(s) 353, 500, 551
Tsp45I GTSAC 1 cut(s) 905
TspDTI ATGAA 2 cut(s) 241, 461
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 2 cut(s) 130, 197
Tth111I GACNNNGTC 1 cut(s) 914
XagI CCTNNNNNAGG 1 cut(s) 729
XceI RCATGY 1 cut(s) 72
XspI CTAG 5 cut(s) 59, 210, 618, 749, 876
Zsp2I ATGCAT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.