inactive purple acid phosphatase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 46, 615 |
| AccB1I | GGYRCC | 4 cut(s) | 46, 146, 615, 979 |
| AciI | CCGC | 1 cut(s) | 60 |
| AclWI | GGATC | 2 cut(s) | 291, 1103 |
| AcoI | YGGCCR | 1 cut(s) | 61 |
| AcuI | CTGAAG | 1 cut(s) | 1077 |
| AcyI | GRCGYC | 2 cut(s) | 147, 240 |
| AfaI | GTAC | 7 cut(s) | 48, 68, 77, 434, 471, 617, 653 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 56, 976 |
| AgsI | TTSAA | 4 cut(s) | 343, 502, 789, 1102 |
| AjnI | CCWGG | 2 cut(s) | 107, 724 |
| AluBI | AGCT | 3 cut(s) | 26, 748, 766 |
| AluI | AGCT | 3 cut(s) | 26, 748, 766 |
| Alw21I | GWGCWC | 1 cut(s) | 625 |
| Alw26I | GTCTC | 2 cut(s) | 176, 745 |
| AlwI | GGATC | 2 cut(s) | 291, 1103 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 26 |
| AoxI | GGCC | 4 cut(s) | 61, 131, 320, 713 |
| ApeKI | GCWGC | 2 cut(s) | 167, 659 |
| AseI | ATTAAT | 1 cut(s) | 368 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 860 |
| Asp718I | GGTACC | 2 cut(s) | 46, 615 |
| AspLEI | GCGC | 2 cut(s) | 149, 942 |
| AspS9I | GGNCC | 1 cut(s) | 714 |
| AsuHPI | GGTGA | 3 cut(s) | 560, 707, 768 |
| AxyI | CCTNAGG | 1 cut(s) | 19 |
| BanI | GGYRCC | 4 cut(s) | 46, 146, 615, 979 |
| BanII | GRGCYC | 1 cut(s) | 462 |
| Bbv12I | GWGCWC | 1 cut(s) | 625 |
| BbvI | GCAGC | 2 cut(s) | 154, 671 |
| BccI | CCATC | 1 cut(s) | 1054 |
| BceAI | ACGGC | 2 cut(s) | 73, 728 |
| BciT130I | CCWGG | 2 cut(s) | 109, 726 |
| BcoDI | GTCTC | 2 cut(s) | 176, 745 |
| BfaI | CTAG | 2 cut(s) | 692, 908 |
| BfmI | CTRYAG | 2 cut(s) | 657, 678 |
| BfoI | RGCGCY | 1 cut(s) | 150 |
| BisI | GCNGC | 3 cut(s) | 61, 168, 660 |
| BlsI | GCNGC | 3 cut(s) | 62, 169, 661 |
| Bme1390I | CCNGG | 2 cut(s) | 109, 726 |
| BmgT120I | GGNCC | 1 cut(s) | 714 |
| BmiI | GGNNCC | 6 cut(s) | 48, 148, 617, 716, 981, 1005 |
| BmrFI | CCNGG | 2 cut(s) | 109, 726 |
| BmsI | GCATC | 3 cut(s) | 499, 751, 906 |
| BpmI | CTGGAG | 1 cut(s) | 609 |
| BpuEI | CTTGAG | 1 cut(s) | 524 |
| BsaBI | GATNNNNATC | 1 cut(s) | 920 |
| BsaHI | GRCGYC | 2 cut(s) | 147, 240 |
| BsaJI | CCNNGG | 3 cut(s) | 55, 724, 793 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 56, 976 |
| Bse118I | RCCGGY | 2 cut(s) | 63, 429 |
| Bse1I | ACTGG | 1 cut(s) | 134 |
| Bse21I | CCTNAGG | 1 cut(s) | 19 |
| Bse3DI | GCAATG | 1 cut(s) | 424 |
| Bse8I | GATNNNNATC | 1 cut(s) | 920 |
| BseBI | CCWGG | 2 cut(s) | 109, 726 |
| BseDI | CCNNGG | 3 cut(s) | 55, 724, 793 |
| BseJI | GATNNNNATC | 1 cut(s) | 920 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 56, 976 |
| BseMI | GCAATG | 1 cut(s) | 424 |
| BseMII | CTCAG | 5 cut(s) | 33, 171, 660, 740, 857 |
| BseNI | ACTGG | 1 cut(s) | 134 |
| BseRI | GAGGAG | 2 cut(s) | 206, 1016 |
| BseX3I | CGGCCG | 1 cut(s) | 61 |
| BseXI | GCAGC | 2 cut(s) | 154, 671 |
| BseYI | CCCAGC | 1 cut(s) | 506 |
| Bsh1285I | CGRYCG | 1 cut(s) | 64 |
| BshFI | GGCC | 4 cut(s) | 63, 133, 322, 715 |
| BshNI | GGYRCC | 4 cut(s) | 46, 146, 615, 979 |
| BsiEI | CGRYCG | 1 cut(s) | 64 |
| BsiHKAI | GWGCWC | 1 cut(s) | 625 |
| BsiSI | CCGG | 2 cut(s) | 64, 430 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 56, 976 |
| BsmAI | GTCTC | 2 cut(s) | 176, 745 |
| BsmI | GAATGC | 2 cut(s) | 529, 687 |
| BsnI | GGCC | 4 cut(s) | 63, 133, 322, 715 |
| Bsp1286I | GDGCHC | 2 cut(s) | 462, 625 |
| Bsp1407I | TGTACA | 1 cut(s) | 469 |
| Bsp143I | GATC | 3 cut(s) | 283, 535, 1108 |
| BspACI | CCGC | 1 cut(s) | 60 |
| BspANI | GGCC | 4 cut(s) | 63, 133, 322, 715 |
| BspCNI | CTCAG | 5 cut(s) | 32, 172, 659, 741, 856 |
| BspHI | TCATGA | 6 cut(s) | 187, 286, 519, 588, 702, 988 |
| BspLI | GGNNCC | 6 cut(s) | 48, 148, 617, 716, 981, 1005 |
| BspMAI | CTGCAG | 1 cut(s) | 661 |
| BspPI | GGATC | 2 cut(s) | 291, 1103 |
| BspT107I | GGYRCC | 4 cut(s) | 46, 146, 615, 979 |
| BsrDI | GCAATG | 1 cut(s) | 424 |
| BsrFI | RCCGGY | 2 cut(s) | 63, 429 |
| BsrGI | TGTACA | 1 cut(s) | 469 |
| BsrI | ACTGG | 1 cut(s) | 134 |
| BssAI | RCCGGY | 2 cut(s) | 63, 429 |
| BssECI | CCNNGG | 3 cut(s) | 55, 724, 793 |
| BssMI | GATC | 3 cut(s) | 283, 535, 1108 |
| BssNI | GRCGYC | 2 cut(s) | 147, 240 |
| BssT1I | CCWWGG | 1 cut(s) | 793 |
| Bst2UI | CCWGG | 2 cut(s) | 109, 726 |
| Bst4CI | ACNGT | 2 cut(s) | 620, 949 |
| Bst6I | CTCTTC | 2 cut(s) | 990, 1053 |
| BstACI | GRCGYC | 2 cut(s) | 147, 240 |
| BstAUI | TGTACA | 1 cut(s) | 469 |
| BstC8I | GCNNGC | 1 cut(s) | 561 |
| BstDEI | CTNAG | 5 cut(s) | 19, 180, 646, 749, 843 |
| BstDSI | CCRYGG | 1 cut(s) | 55 |
| BstH2I | RGCGCY | 1 cut(s) | 150 |
| BstHHI | GCGC | 2 cut(s) | 149, 942 |
| BstKTI | GATC | 3 cut(s) | 286, 538, 1111 |
| BstMAI | GTCTC | 2 cut(s) | 176, 745 |
| BstMBI | GATC | 3 cut(s) | 283, 535, 1108 |
| BstMCI | CGRYCG | 1 cut(s) | 64 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 23, 176 |
| BstNI | CCWGG | 2 cut(s) | 109, 726 |
| BstSCI | CCNGG | 2 cut(s) | 107, 724 |
| BstSFI | CTRYAG | 2 cut(s) | 657, 678 |
| BstV1I | GCAGC | 2 cut(s) | 154, 671 |
| BstX2I | RGATCY | 1 cut(s) | 1108 |
| BstYI | RGATCY | 1 cut(s) | 1108 |
| BstZI | CGGCCG | 1 cut(s) | 61 |
| Bsu36I | CCTNAGG | 1 cut(s) | 19 |
| BsuRI | GGCC | 4 cut(s) | 63, 133, 322, 715 |
| BtgI | CCRYGG | 1 cut(s) | 55 |
| BtsI | GCAGTG | 2 cut(s) | 12, 654 |
| BtsIMutI | CAGTG | 2 cut(s) | 12, 654 |
| Cac8I | GCNNGC | 1 cut(s) | 561 |
| CaiI | CAGNNNCTG | 1 cut(s) | 26 |
| CciI | TCATGA | 6 cut(s) | 187, 286, 519, 588, 702, 988 |
| CfoI | GCGC | 2 cut(s) | 149, 942 |
| Cfr10I | RCCGGY | 2 cut(s) | 63, 429 |
| Cfr13I | GGNCC | 1 cut(s) | 714 |
| CseI | GACGC | 1 cut(s) | 248 |
| Csp6I | GTAC | 7 cut(s) | 47, 67, 76, 433, 470, 616, 652 |
| CviAII | CATG | 7 cut(s) | 188, 280, 287, 520, 589, 703, 989 |
| CviQI | GTAC | 7 cut(s) | 47, 67, 76, 433, 470, 616, 652 |
| DdeI | CTNAG | 5 cut(s) | 19, 180, 646, 749, 843 |
| DinI | GGCGCC | 1 cut(s) | 148 |
| DpnI | GATC | 3 cut(s) | 285, 537, 1110 |
| DpnII | GATC | 3 cut(s) | 283, 535, 1108 |
| EaeI | YGGCCR | 1 cut(s) | 61 |
| EagI | CGGCCG | 1 cut(s) | 61 |
| Eam1104I | CTCTTC | 2 cut(s) | 990, 1053 |
| EarI | CTCTTC | 2 cut(s) | 990, 1053 |
| EclXI | CGGCCG | 1 cut(s) | 61 |
| Eco130I | CCWWGG | 1 cut(s) | 793 |
| Eco147I | AGGCCT | 1 cut(s) | 322 |
| Eco24I | GRGCYC | 1 cut(s) | 462 |
| Eco32I | GATATC | 1 cut(s) | 994 |
| Eco52I | CGGCCG | 1 cut(s) | 61 |
| Eco57I | CTGAAG | 1 cut(s) | 1077 |
| Eco81I | CCTNAGG | 1 cut(s) | 19 |
| EcoRII | CCWGG | 2 cut(s) | 107, 724 |
| EcoRV | GATATC | 1 cut(s) | 994 |
| EcoT14I | CCWWGG | 1 cut(s) | 793 |
| EcoT22I | ATGCAT | 1 cut(s) | 223 |
| EcoT38I | GRGCYC | 1 cut(s) | 462 |
| EgeI | GGCGCC | 1 cut(s) | 148 |
| EheI | GGCGCC | 1 cut(s) | 148 |
| ErhI | CCWWGG | 1 cut(s) | 793 |
| FaeI | CATG | 7 cut(s) | 191, 283, 290, 523, 592, 706, 992 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 323, 355, 845, 877 |
| FatI | CATG | 7 cut(s) | 187, 279, 286, 519, 588, 702, 988 |
| FauNDI | CATATG | 1 cut(s) | 1079 |
| Fnu4HI | GCNGC | 3 cut(s) | 61, 168, 660 |
| FriOI | GRGCYC | 1 cut(s) | 462 |
| Fsp4HI | GCNGC | 3 cut(s) | 61, 168, 660 |
| FspBI | CTAG | 2 cut(s) | 692, 908 |
| GlaI | GCGC | 2 cut(s) | 148, 941 |
| GluI | GCNGC | 3 cut(s) | 61, 168, 660 |
| GsaI | CCCAGC | 1 cut(s) | 510 |
| GsuI | CTGGAG | 1 cut(s) | 609 |
| HaeII | RGCGCY | 1 cut(s) | 150 |
| HaeIII | GGCC | 4 cut(s) | 63, 133, 322, 715 |
| HapII | CCGG | 2 cut(s) | 64, 430 |
| HgaI | GACGC | 1 cut(s) | 248 |
| HhaI | GCGC | 2 cut(s) | 149, 942 |
| Hin1I | GRCGYC | 2 cut(s) | 147, 240 |
| Hin1II | CATG | 7 cut(s) | 191, 283, 290, 523, 592, 706, 992 |
| Hin6I | GCGC | 2 cut(s) | 147, 940 |
| HinP1I | GCGC | 2 cut(s) | 147, 940 |
| HinfI | GANTC | 5 cut(s) | 91, 184, 753, 785, 1088 |
| HpaII | CCGG | 2 cut(s) | 64, 430 |
| HphI | GGTGA | 3 cut(s) | 560, 707, 768 |
| Hpy166II | GTNNAC | 4 cut(s) | 175, 352, 654, 776 |
| Hpy188I | TCNGA | 5 cut(s) | 41, 390, 577, 649, 1057 |
| Hpy8I | GTNNAC | 4 cut(s) | 175, 352, 654, 776 |
| HpyAV | CCTTC | 3 cut(s) | 325, 392, 850 |
| HpyCH4III | ACNGT | 2 cut(s) | 620, 949 |
| HpyCH4IV | ACGT | 1 cut(s) | 69 |
| HpyCH4V | TGCA | 8 cut(s) | 221, 490, 527, 563, 659, 687, 742, 851 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 23, 176 |
| HpyF3I | CTNAG | 5 cut(s) | 19, 180, 646, 749, 843 |
| HpySE526I | ACGT | 1 cut(s) | 69 |
| Hsp92I | GRCGYC | 2 cut(s) | 147, 240 |
| Hsp92II | CATG | 7 cut(s) | 191, 283, 290, 523, 592, 706, 992 |
| HspAI | GCGC | 2 cut(s) | 147, 940 |
| KasI | GGCGCC | 1 cut(s) | 146 |
| KpnI | GGTACC | 2 cut(s) | 50, 619 |
| Kzo9I | GATC | 3 cut(s) | 283, 535, 1108 |
| LmnI | GCTCC | 5 cut(s) | 23, 404, 628, 639, 1003 |
| Lsp1109I | GCAGC | 2 cut(s) | 154, 671 |
| LweI | GCATC | 3 cut(s) | 499, 751, 906 |
| MaeI | CTAG | 2 cut(s) | 692, 908 |
| MaeII | ACGT | 1 cut(s) | 69 |
| MaeIII | GTNAC | 2 cut(s) | 450, 604 |
| MalI | GATC | 3 cut(s) | 285, 537, 1110 |
| MboI | GATC | 3 cut(s) | 283, 535, 1108 |
| MboII | GAAGA | 6 cut(s) | 220, 263, 834, 925, 977, 1070 |
| MfeI | CAATTG | 1 cut(s) | 663 |
| MflI | RGATCY | 1 cut(s) | 1108 |
| MhlI | GDGCHC | 2 cut(s) | 462, 625 |
| MluCI | AATT | 7 cut(s) | 261, 365, 511, 529, 640, 663, 1113 |
| Mly113I | GGCGCC | 1 cut(s) | 147 |
| MlyI | GAGTC | 3 cut(s) | 178, 747, 1097 |
| MmeI | TCCRAC | 2 cut(s) | 426, 850 |
| Mph1103I | ATGCAT | 1 cut(s) | 223 |
| MroXI | GAANNNNTTC | 1 cut(s) | 860 |
| MseI | TTAA | 5 cut(s) | 204, 264, 306, 368, 639 |
| MslI | CAYNNNNRTG | 1 cut(s) | 291 |
| MspA1I | CMGCKG | 1 cut(s) | 748 |
| MspI | CCGG | 2 cut(s) | 64, 430 |
| MspR9I | CCNGG | 2 cut(s) | 109, 726 |
| MunI | CAATTG | 1 cut(s) | 663 |
| Mva1269I | GAATGC | 2 cut(s) | 529, 687 |
| MvaI | CCWGG | 2 cut(s) | 109, 726 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 23, 176 |
| NarI | GGCGCC | 1 cut(s) | 147 |
| NdeI | CATATG | 1 cut(s) | 1079 |
| NdeII | GATC | 3 cut(s) | 283, 535, 1108 |
| NlaIII | CATG | 7 cut(s) | 191, 283, 290, 523, 592, 706, 992 |
| NlaIV | GGNNCC | 6 cut(s) | 48, 148, 617, 716, 981, 1005 |
| NsiI | ATGCAT | 1 cut(s) | 223 |
| PagI | TCATGA | 6 cut(s) | 187, 286, 519, 588, 702, 988 |
| PceI | AGGCCT | 1 cut(s) | 322 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 616 |
| PctI | GAATGC | 2 cut(s) | 529, 687 |
| PdmI | GAANNNNTTC | 1 cut(s) | 860 |
| PfeI | GAWTC | 2 cut(s) | 91, 785 |
| PfoI | TCCNGGA | 1 cut(s) | 107 |
| PkrI | GCNGC | 3 cut(s) | 62, 169, 661 |
| PleI | GAGTC | 3 cut(s) | 178, 747, 1096 |
| PluTI | GGCGCC | 1 cut(s) | 150 |
| PpsI | GAGTC | 3 cut(s) | 178, 747, 1096 |
| PshBI | ATTAAT | 1 cut(s) | 368 |
| Psp6I | CCWGG | 2 cut(s) | 107, 724 |
| PspFI | CCCAGC | 1 cut(s) | 506 |
| PspGI | CCWGG | 2 cut(s) | 107, 724 |
| PspN4I | GGNNCC | 6 cut(s) | 48, 148, 617, 716, 981, 1005 |
| PspPI | GGNCC | 1 cut(s) | 714 |
| PstI | CTGCAG | 1 cut(s) | 661 |
| PstNI | CAGNNNCTG | 1 cut(s) | 26 |
| PsuI | RGATCY | 1 cut(s) | 1108 |
| PvuII | CAGCTG | 1 cut(s) | 748 |
| RsaI | GTAC | 7 cut(s) | 48, 68, 77, 434, 471, 617, 653 |
| RsaNI | GTAC | 7 cut(s) | 47, 67, 76, 433, 470, 616, 652 |
| RseI | CAYNNNNRTG | 1 cut(s) | 291 |
| SaqAI | TTAA | 5 cut(s) | 204, 264, 306, 368, 639 |
| SatI | GCNGC | 3 cut(s) | 61, 168, 660 |
| Sau3AI | GATC | 3 cut(s) | 283, 535, 1108 |
| Sau96I | GGNCC | 1 cut(s) | 714 |
| SchI | GAGTC | 3 cut(s) | 178, 747, 1097 |
| ScrFI | CCNGG | 2 cut(s) | 109, 726 |
| SduI | GDGCHC | 2 cut(s) | 462, 625 |
| SfaNI | GCATC | 3 cut(s) | 499, 751, 906 |
| SfcI | CTRYAG | 2 cut(s) | 657, 678 |
| SfoI | GGCGCC | 1 cut(s) | 148 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 291 |
| SmlI | CTYRAG | 1 cut(s) | 539 |
| SmoI | CTYRAG | 1 cut(s) | 539 |
| Sse9I | AATT | 7 cut(s) | 261, 365, 511, 529, 640, 663, 1113 |
| SseBI | AGGCCT | 1 cut(s) | 322 |
| SsiI | CCGC | 1 cut(s) | 60 |
| SspDI | GGCGCC | 1 cut(s) | 146 |
| SspMI | CTAG | 2 cut(s) | 692, 908 |
| StuI | AGGCCT | 1 cut(s) | 322 |
| StyD4I | CCNGG | 2 cut(s) | 107, 724 |
| StyI | CCWWGG | 1 cut(s) | 793 |
| TaaI | ACNGT | 2 cut(s) | 620, 949 |
| TaiI | ACGT | 1 cut(s) | 72 |
| TaqI | TCGA | 1 cut(s) | 832 |
| TaqII | GACCGA | 1 cut(s) | 608 |
| TasI | AATT | 7 cut(s) | 261, 365, 511, 529, 640, 663, 1113 |
| TatI | WGTACW | 3 cut(s) | 75, 469, 651 |
| TauI | GCSGC | 1 cut(s) | 63 |
| TfiI | GAWTC | 2 cut(s) | 91, 785 |
| Tru1I | TTAA | 5 cut(s) | 204, 264, 306, 368, 639 |
| Tru9I | TTAA | 5 cut(s) | 204, 264, 306, 368, 639 |
| TscAI | CASTG | 2 cut(s) | 19, 661 |
| TseI | GCWGC | 2 cut(s) | 167, 659 |
| TspDTI | ATGAA | 6 cut(s) | 303, 374, 378, 536, 577, 977 |
| TspGWI | ACGGA | 1 cut(s) | 856 |
| TspRI | CASTG | 2 cut(s) | 19, 661 |
| VspI | ATTAAT | 1 cut(s) | 368 |
| XmnI | GAANNNNTTC | 1 cut(s) | 860 |
| XspI | CTAG | 2 cut(s) | 692, 908 |
| Zsp2I | ATGCAT | 1 cut(s) | 223 |