WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 337, 695 |
| AccI | GTMKAC | 1 cut(s) | 202 |
| AccIII | TCCGGA | 1 cut(s) | 707 |
| AciI | CCGC | 2 cut(s) | 405, 899 |
| AclWI | GGATC | 1 cut(s) | 534 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcuI | CTGAAG | 1 cut(s) | 776 |
| AfaI | GTAC | 5 cut(s) | 275, 366, 420, 427, 508 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 255 |
| AgsI | TTSAA | 3 cut(s) | 394, 582, 1116 |
| AhlI | ACTAGT | 1 cut(s) | 728 |
| AleI | CACNNNNGTG | 1 cut(s) | 433 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 562, 594 |
| AluBI | AGCT | 5 cut(s) | 180, 541, 587, 649, 945 |
| AluI | AGCT | 5 cut(s) | 180, 541, 587, 649, 945 |
| Alw21I | GWGCWC | 1 cut(s) | 728 |
| Alw26I | GTCTC | 1 cut(s) | 1102 |
| Alw44I | GTGCAC | 1 cut(s) | 724 |
| AlwI | GGATC | 1 cut(s) | 534 |
| Aor13HI | TCCGGA | 1 cut(s) | 707 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApaLI | GTGCAC | 1 cut(s) | 724 |
| ApeKI | GCWGC | 4 cut(s) | 449, 546, 866, 896 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 20, 38 |
| AspS9I | GGNCC | 1 cut(s) | 851 |
| AsuC2I | CCSGG | 2 cut(s) | 7, 793 |
| AsuHPI | GGTGA | 2 cut(s) | 397, 913 |
| AsuII | TTCGAA | 1 cut(s) | 494 |
| AvaII | GGWCC | 1 cut(s) | 851 |
| BaeGI | GKGCMC | 1 cut(s) | 728 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 417, 450 |
| BanI | GGYRCC | 2 cut(s) | 337, 695 |
| BbsI | GAAGAC | 1 cut(s) | 606 |
| Bbv12I | GWGCWC | 1 cut(s) | 728 |
| BbvI | GCAGC | 4 cut(s) | 461, 558, 878, 908 |
| BccI | CCATC | 3 cut(s) | 167, 517, 561 |
| BciVI | GTATCC | 1 cut(s) | 920 |
| BclI | TGATCA | 1 cut(s) | 487 |
| BcnI | CCSGG | 2 cut(s) | 7, 793 |
| BcoDI | GTCTC | 1 cut(s) | 1102 |
| BcuI | ACTAGT | 1 cut(s) | 728 |
| BfaI | CTAG | 3 cut(s) | 626, 729, 1031 |
| BfmI | CTRYAG | 1 cut(s) | 864 |
| BfuI | GTATCC | 1 cut(s) | 920 |
| BisI | GCNGC | 4 cut(s) | 450, 547, 867, 897 |
| BlpI | GCTNAGC | 1 cut(s) | 542 |
| BlsI | GCNGC | 4 cut(s) | 451, 548, 868, 898 |
| BmcAI | AGTACT | 3 cut(s) | 275, 420, 508 |
| Bme1390I | CCNGG | 2 cut(s) | 7, 793 |
| Bme18I | GGWCC | 1 cut(s) | 851 |
| BmgT120I | GGNCC | 1 cut(s) | 851 |
| BmiI | GGNNCC | 4 cut(s) | 171, 339, 697, 852 |
| BmrFI | CCNGG | 2 cut(s) | 7, 793 |
| BmsI | GCATC | 1 cut(s) | 812 |
| BpiI | GAAGAC | 1 cut(s) | 606 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 542 |
| Bpu14I | TTCGAA | 1 cut(s) | 494 |
| BpuEI | CTTGAG | 2 cut(s) | 77, 262 |
| BpuMI | CCSGG | 2 cut(s) | 7, 793 |
| BsaAI | YACGTR | 1 cut(s) | 138 |
| BsaBI | GATNNNNATC | 1 cut(s) | 908 |
| BsaJI | CCNNGG | 4 cut(s) | 6, 218, 286, 937 |
| BsaWI | WCCGGW | 1 cut(s) | 707 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 255 |
| Bse1I | ACTGG | 2 cut(s) | 435, 1082 |
| Bse3DI | GCAATG | 1 cut(s) | 42 |
| Bse8I | GATNNNNATC | 1 cut(s) | 908 |
| BseAI | TCCGGA | 1 cut(s) | 707 |
| BseDI | CCNNGG | 4 cut(s) | 6, 218, 286, 937 |
| BseGI | GGATG | 3 cut(s) | 260, 801, 831 |
| BseJI | GATNNNNATC | 1 cut(s) | 908 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 255 |
| BseMI | GCAATG | 1 cut(s) | 42 |
| BseMII | CTCAG | 1 cut(s) | 533 |
| BseNI | ACTGG | 2 cut(s) | 435, 1082 |
| BseRI | GAGGAG | 1 cut(s) | 1065 |
| BseSI | GKGCMC | 1 cut(s) | 728 |
| BseXI | GCAGC | 4 cut(s) | 461, 558, 878, 908 |
| BsgI | GTGCAG | 1 cut(s) | 468 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BshNI | GGYRCC | 2 cut(s) | 337, 695 |
| BsiHKAI | GWGCWC | 1 cut(s) | 728 |
| BsiSI | CCGG | 3 cut(s) | 6, 708, 792 |
| BslFI | GGGAC | 1 cut(s) | 864 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 255 |
| BsmAI | GTCTC | 1 cut(s) | 1102 |
| BsmFI | GGGAC | 1 cut(s) | 864 |
| BsnI | GGCC | 1 cut(s) | 5 |
| Bsp119I | TTCGAA | 1 cut(s) | 494 |
| Bsp1286I | GDGCHC | 1 cut(s) | 728 |
| Bsp13I | TCCGGA | 1 cut(s) | 707 |
| Bsp143I | GATC | 3 cut(s) | 487, 526, 903 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 542 |
| BspACI | CCGC | 2 cut(s) | 405, 899 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BspCNI | CTCAG | 1 cut(s) | 534 |
| BspEI | TCCGGA | 1 cut(s) | 707 |
| BspHI | TCATGA | 1 cut(s) | 922 |
| BspLI | GGNNCC | 4 cut(s) | 171, 339, 697, 852 |
| BspMAI | CTGCAG | 1 cut(s) | 868 |
| BspPI | GGATC | 1 cut(s) | 534 |
| BspT104I | TTCGAA | 1 cut(s) | 494 |
| BspT107I | GGYRCC | 2 cut(s) | 337, 695 |
| BsrDI | GCAATG | 1 cut(s) | 42 |
| BsrI | ACTGG | 2 cut(s) | 435, 1082 |
| BssECI | CCNNGG | 4 cut(s) | 6, 218, 286, 937 |
| BssMI | GATC | 3 cut(s) | 487, 526, 903 |
| BssT1I | CCWWGG | 3 cut(s) | 218, 286, 937 |
| Bst4CI | ACNGT | 4 cut(s) | 273, 574, 606, 781 |
| Bst6I | CTCTTC | 1 cut(s) | 242 |
| BstBAI | YACGTR | 1 cut(s) | 138 |
| BstBI | TTCGAA | 1 cut(s) | 494 |
| BstC8I | GCNNGC | 1 cut(s) | 647 |
| BstDEI | CTNAG | 2 cut(s) | 542, 715 |
| BstF5I | GGATG | 3 cut(s) | 260, 801, 831 |
| BstKTI | GATC | 3 cut(s) | 490, 529, 906 |
| BstMAI | GTCTC | 1 cut(s) | 1102 |
| BstMBI | GATC | 3 cut(s) | 487, 526, 903 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 53, 314, 593, 1031, 1040 |
| BstSCI | CCNGG | 2 cut(s) | 5, 791 |
| BstSFI | CTRYAG | 1 cut(s) | 864 |
| BstSLI | GKGCMC | 1 cut(s) | 728 |
| BstSNI | TACGTA | 1 cut(s) | 138 |
| BstV1I | GCAGC | 4 cut(s) | 461, 558, 878, 908 |
| BstV2I | GAAGAC | 1 cut(s) | 606 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 166 |
| BsuI | GTATCC | 1 cut(s) | 920 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BtgZI | GCGATG | 1 cut(s) | 1064 |
| BtsCI | GGATG | 3 cut(s) | 260, 801, 831 |
| BtsIMutI | CAGTG | 3 cut(s) | 428, 602, 777 |
| Cac8I | GCNNGC | 1 cut(s) | 647 |
| CciI | TCATGA | 1 cut(s) | 922 |
| Cfr13I | GGNCC | 1 cut(s) | 851 |
| Csp6I | GTAC | 5 cut(s) | 274, 365, 419, 426, 507 |
| CviQI | GTAC | 5 cut(s) | 274, 365, 419, 426, 507 |
| DdeI | CTNAG | 2 cut(s) | 542, 715 |
| DpnI | GATC | 3 cut(s) | 489, 528, 905 |
| DpnII | GATC | 3 cut(s) | 487, 526, 903 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 1 cut(s) | 242 |
| EarI | CTCTTC | 1 cut(s) | 242 |
| Eco105I | TACGTA | 1 cut(s) | 138 |
| Eco130I | CCWWGG | 3 cut(s) | 218, 286, 937 |
| Eco47I | GGWCC | 1 cut(s) | 851 |
| Eco57I | CTGAAG | 1 cut(s) | 776 |
| EcoO109I | RGGNCCY | 1 cut(s) | 851 |
| EcoT14I | CCWWGG | 3 cut(s) | 218, 286, 937 |
| ErhI | CCWWGG | 3 cut(s) | 218, 286, 937 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 374, 406 |
| FaqI | GGGAC | 1 cut(s) | 864 |
| FbaI | TGATCA | 1 cut(s) | 487 |
| FblI | GTMKAC | 1 cut(s) | 202 |
| Fnu4HI | GCNGC | 4 cut(s) | 450, 547, 867, 897 |
| FokI | GGATG | 3 cut(s) | 247, 808, 838 |
| Fsp4HI | GCNGC | 4 cut(s) | 450, 547, 867, 897 |
| FspBI | CTAG | 3 cut(s) | 626, 729, 1031 |
| GluI | GCNGC | 4 cut(s) | 450, 547, 867, 897 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HapII | CCGG | 3 cut(s) | 6, 708, 792 |
| HincII | GTYRAC | 2 cut(s) | 147, 331 |
| HindII | GTYRAC | 2 cut(s) | 147, 331 |
| HindIII | AAGCTT | 1 cut(s) | 943 |
| HinfI | GANTC | 5 cut(s) | 21, 291, 711, 1095, 1112 |
| HpaII | CCGG | 3 cut(s) | 6, 708, 792 |
| HphI | GGTGA | 2 cut(s) | 397, 913 |
| Hpy166II | GTNNAC | 7 cut(s) | 147, 203, 331, 388, 428, 726, 861 |
| Hpy188I | TCNGA | 3 cut(s) | 756, 888, 1094 |
| Hpy188III | TCNNGA | 4 cut(s) | 94, 241, 708, 923 |
| Hpy8I | GTNNAC | 7 cut(s) | 147, 203, 331, 388, 428, 726, 861 |
| Hpy99I | CGWCG | 1 cut(s) | 29 |
| HpyAV | CCTTC | 6 cut(s) | 10, 104, 376, 751, 1048, 1080 |
| HpyCH4III | ACNGT | 4 cut(s) | 273, 574, 606, 781 |
| HpyCH4IV | ACGT | 1 cut(s) | 137 |
| HpyCH4V | TGCA | 7 cut(s) | 399, 449, 596, 631, 726, 866, 1012 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 53, 314, 593, 1031, 1040 |
| HpyF3I | CTNAG | 2 cut(s) | 542, 715 |
| HpySE526I | ACGT | 1 cut(s) | 137 |
| Kpn2I | TCCGGA | 1 cut(s) | 707 |
| Ksp22I | TGATCA | 1 cut(s) | 487 |
| Kzo9I | GATC | 3 cut(s) | 487, 526, 903 |
| LmnI | GCTCC | 3 cut(s) | 175, 1057, 1063 |
| Lsp1109I | GCAGC | 4 cut(s) | 461, 558, 878, 908 |
| LweI | GCATC | 1 cut(s) | 812 |
| MaeI | CTAG | 3 cut(s) | 626, 729, 1031 |
| MaeII | ACGT | 1 cut(s) | 137 |
| MaeIII | GTNAC | 2 cut(s) | 230, 346 |
| MalI | GATC | 3 cut(s) | 489, 528, 905 |
| MboI | GATC | 3 cut(s) | 487, 526, 903 |
| MboII | GAAGA | 5 cut(s) | 154, 229, 606, 853, 977 |
| MhlI | GDGCHC | 1 cut(s) | 728 |
| MluCI | AATT | 4 cut(s) | 150, 174, 513, 969 |
| MlyI | GAGTC | 1 cut(s) | 1104 |
| MnlI | CCTC | 3 cut(s) | 245, 749, 1086 |
| MroI | TCCGGA | 1 cut(s) | 707 |
| MroXI | GAANNNNTTC | 2 cut(s) | 20, 38 |
| MslI | CAYNNNNRTG | 4 cut(s) | 47, 433, 607, 782 |
| MspA1I | CMGCKG | 1 cut(s) | 899 |
| MspI | CCGG | 3 cut(s) | 6, 708, 792 |
| MspR9I | CCNGG | 2 cut(s) | 7, 793 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 53, 314, 593, 1031, 1040 |
| NciI | CCSGG | 2 cut(s) | 7, 793 |
| NdeII | GATC | 3 cut(s) | 487, 526, 903 |
| NlaIV | GGNNCC | 4 cut(s) | 171, 339, 697, 852 |
| NmuCI | GTSAC | 1 cut(s) | 346 |
| NspV | TTCGAA | 1 cut(s) | 494 |
| OliI | CACNNNNGTG | 1 cut(s) | 433 |
| PagI | TCATGA | 1 cut(s) | 922 |
| PdmI | GAANNNNTTC | 2 cut(s) | 20, 38 |
| PfeI | GAWTC | 4 cut(s) | 21, 291, 711, 1112 |
| PkrI | GCNGC | 4 cut(s) | 451, 548, 868, 898 |
| PleI | GAGTC | 1 cut(s) | 1103 |
| PpsI | GAGTC | 1 cut(s) | 1103 |
| Ppu21I | YACGTR | 1 cut(s) | 138 |
| PpuMI | RGGWCCY | 1 cut(s) | 851 |
| Psp5II | RGGWCCY | 1 cut(s) | 851 |
| PspN4I | GGNNCC | 4 cut(s) | 171, 339, 697, 852 |
| PspPI | GGNCC | 1 cut(s) | 851 |
| PspPPI | RGGWCCY | 1 cut(s) | 851 |
| PstI | CTGCAG | 1 cut(s) | 868 |
| RsaI | GTAC | 5 cut(s) | 275, 366, 420, 427, 508 |
| RsaNI | GTAC | 5 cut(s) | 274, 365, 419, 426, 507 |
| RseI | CAYNNNNRTG | 4 cut(s) | 47, 433, 607, 782 |
| SatI | GCNGC | 4 cut(s) | 450, 547, 867, 897 |
| Sau3AI | GATC | 3 cut(s) | 487, 526, 903 |
| Sau96I | GGNCC | 1 cut(s) | 851 |
| ScaI | AGTACT | 3 cut(s) | 275, 420, 508 |
| SchI | GAGTC | 1 cut(s) | 1104 |
| ScrFI | CCNGG | 2 cut(s) | 7, 793 |
| SduI | GDGCHC | 1 cut(s) | 728 |
| SfaNI | GCATC | 1 cut(s) | 812 |
| SfcI | CTRYAG | 1 cut(s) | 864 |
| SfuI | TTCGAA | 1 cut(s) | 494 |
| SinI | GGWCC | 1 cut(s) | 851 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 47, 433, 607, 782 |
| SmlI | CTYRAG | 2 cut(s) | 92, 241 |
| SmoI | CTYRAG | 2 cut(s) | 92, 241 |
| SnaBI | TACGTA | 1 cut(s) | 138 |
| SpeI | ACTAGT | 1 cut(s) | 728 |
| Sse9I | AATT | 4 cut(s) | 150, 174, 513, 969 |
| SsiI | CCGC | 2 cut(s) | 405, 899 |
| SspI | AATATT | 1 cut(s) | 85 |
| SspMI | CTAG | 3 cut(s) | 626, 729, 1031 |
| StyD4I | CCNGG | 2 cut(s) | 5, 791 |
| StyI | CCWWGG | 3 cut(s) | 218, 286, 937 |
| TaaI | ACNGT | 4 cut(s) | 273, 574, 606, 781 |
| TaiI | ACGT | 1 cut(s) | 140 |
| TaqI | TCGA | 3 cut(s) | 24, 155, 494 |
| TasI | AATT | 4 cut(s) | 150, 174, 513, 969 |
| TatI | WGTACW | 3 cut(s) | 273, 418, 506 |
| TfiI | GAWTC | 4 cut(s) | 21, 291, 711, 1112 |
| TscAI | CASTG | 3 cut(s) | 435, 609, 784 |
| TseFI | GTSAC | 1 cut(s) | 346 |
| TseI | GCWGC | 4 cut(s) | 449, 546, 866, 896 |
| Tsp45I | GTSAC | 1 cut(s) | 346 |
| TspGWI | ACGGA | 2 cut(s) | 733, 1090 |
| TspRI | CASTG | 3 cut(s) | 435, 609, 784 |
| VneI | GTGCAC | 1 cut(s) | 724 |
| VpaK11BI | GGWCC | 1 cut(s) | 851 |
| XmiI | GTMKAC | 1 cut(s) | 202 |
| XmnI | GAANNNNTTC | 2 cut(s) | 20, 38 |
| XspI | CTAG | 3 cut(s) | 626, 729, 1031 |
| ZrmI | AGTACT | 3 cut(s) | 275, 420, 508 |