WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 325, 683 |
| AccI | GTMKAC | 1 cut(s) | 190 |
| AccIII | TCCGGA | 1 cut(s) | 695 |
| AciI | CCGC | 2 cut(s) | 393, 887 |
| AclWI | GGATC | 1 cut(s) | 522 |
| AfaI | GTAC | 5 cut(s) | 263, 354, 408, 415, 496 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 243 |
| AgsI | TTSAA | 3 cut(s) | 382, 570, 1104 |
| AhlI | ACTAGT | 1 cut(s) | 716 |
| AleI | CACNNNNGTG | 1 cut(s) | 421 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 550, 582 |
| AluBI | AGCT | 5 cut(s) | 168, 529, 575, 637, 933 |
| AluI | AGCT | 5 cut(s) | 168, 529, 575, 637, 933 |
| Alw21I | GWGCWC | 1 cut(s) | 716 |
| Alw26I | GTCTC | 1 cut(s) | 1090 |
| Alw44I | GTGCAC | 1 cut(s) | 712 |
| AlwI | GGATC | 1 cut(s) | 522 |
| Aor13HI | TCCGGA | 1 cut(s) | 695 |
| ApaLI | GTGCAC | 1 cut(s) | 712 |
| ApeKI | GCWGC | 4 cut(s) | 437, 534, 854, 884 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 8, 26 |
| AspS9I | GGNCC | 1 cut(s) | 839 |
| AsuC2I | CCSGG | 1 cut(s) | 781 |
| AsuHPI | GGTGA | 2 cut(s) | 385, 901 |
| AsuII | TTCGAA | 1 cut(s) | 482 |
| AvaII | GGWCC | 1 cut(s) | 839 |
| BaeGI | GKGCMC | 1 cut(s) | 716 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 405, 438 |
| BanI | GGYRCC | 2 cut(s) | 325, 683 |
| BbsI | GAAGAC | 1 cut(s) | 594 |
| Bbv12I | GWGCWC | 1 cut(s) | 716 |
| BbvI | GCAGC | 4 cut(s) | 449, 546, 866, 896 |
| BccI | CCATC | 3 cut(s) | 155, 505, 549 |
| BciVI | GTATCC | 1 cut(s) | 908 |
| BclI | TGATCA | 2 cut(s) | 475, 1074 |
| BcnI | CCSGG | 1 cut(s) | 781 |
| BcoDI | GTCTC | 1 cut(s) | 1090 |
| BcuI | ACTAGT | 1 cut(s) | 716 |
| BfaI | CTAG | 3 cut(s) | 614, 717, 1019 |
| BfmI | CTRYAG | 1 cut(s) | 852 |
| BfuI | GTATCC | 1 cut(s) | 908 |
| BisI | GCNGC | 4 cut(s) | 438, 535, 855, 885 |
| BlpI | GCTNAGC | 1 cut(s) | 530 |
| BlsI | GCNGC | 4 cut(s) | 439, 536, 856, 886 |
| BmcAI | AGTACT | 3 cut(s) | 263, 408, 496 |
| Bme1390I | CCNGG | 1 cut(s) | 781 |
| Bme18I | GGWCC | 1 cut(s) | 839 |
| BmgT120I | GGNCC | 1 cut(s) | 839 |
| BmiI | GGNNCC | 4 cut(s) | 159, 327, 685, 840 |
| BmrFI | CCNGG | 1 cut(s) | 781 |
| BmsI | GCATC | 1 cut(s) | 800 |
| BpiI | GAAGAC | 1 cut(s) | 594 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 530 |
| Bpu14I | TTCGAA | 1 cut(s) | 482 |
| BpuEI | CTTGAG | 2 cut(s) | 65, 250 |
| BpuMI | CCSGG | 1 cut(s) | 781 |
| BsaAI | YACGTR | 1 cut(s) | 126 |
| BsaBI | GATNNNNATC | 1 cut(s) | 896 |
| BsaJI | CCNNGG | 3 cut(s) | 206, 274, 925 |
| BsaWI | WCCGGW | 1 cut(s) | 695 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 243 |
| Bse1I | ACTGG | 2 cut(s) | 423, 1070 |
| Bse3DI | GCAATG | 1 cut(s) | 30 |
| Bse8I | GATNNNNATC | 1 cut(s) | 896 |
| BseAI | TCCGGA | 1 cut(s) | 695 |
| BseDI | CCNNGG | 3 cut(s) | 206, 274, 925 |
| BseGI | GGATG | 3 cut(s) | 248, 789, 819 |
| BseJI | GATNNNNATC | 1 cut(s) | 896 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 243 |
| BseMI | GCAATG | 1 cut(s) | 30 |
| BseMII | CTCAG | 2 cut(s) | 521, 734 |
| BseNI | ACTGG | 2 cut(s) | 423, 1070 |
| BseRI | GAGGAG | 1 cut(s) | 1053 |
| BseSI | GKGCMC | 1 cut(s) | 716 |
| BseXI | GCAGC | 4 cut(s) | 449, 546, 866, 896 |
| BsgI | GTGCAG | 1 cut(s) | 456 |
| BshNI | GGYRCC | 2 cut(s) | 325, 683 |
| BsiHKAI | GWGCWC | 1 cut(s) | 716 |
| BsiSI | CCGG | 2 cut(s) | 696, 780 |
| BslFI | GGGAC | 1 cut(s) | 852 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 243 |
| BsmAI | GTCTC | 1 cut(s) | 1090 |
| BsmFI | GGGAC | 1 cut(s) | 852 |
| Bsp119I | TTCGAA | 1 cut(s) | 482 |
| Bsp1286I | GDGCHC | 1 cut(s) | 716 |
| Bsp13I | TCCGGA | 1 cut(s) | 695 |
| Bsp143I | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 530 |
| BspACI | CCGC | 2 cut(s) | 393, 887 |
| BspCNI | CTCAG | 2 cut(s) | 522, 735 |
| BspEI | TCCGGA | 1 cut(s) | 695 |
| BspHI | TCATGA | 1 cut(s) | 910 |
| BspLI | GGNNCC | 4 cut(s) | 159, 327, 685, 840 |
| BspMAI | CTGCAG | 1 cut(s) | 856 |
| BspPI | GGATC | 1 cut(s) | 522 |
| BspT104I | TTCGAA | 1 cut(s) | 482 |
| BspT107I | GGYRCC | 2 cut(s) | 325, 683 |
| BsrDI | GCAATG | 1 cut(s) | 30 |
| BsrI | ACTGG | 2 cut(s) | 423, 1070 |
| BssECI | CCNNGG | 3 cut(s) | 206, 274, 925 |
| BssMI | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| BssT1I | CCWWGG | 3 cut(s) | 206, 274, 925 |
| Bst4CI | ACNGT | 4 cut(s) | 261, 562, 594, 769 |
| Bst6I | CTCTTC | 1 cut(s) | 230 |
| BstBAI | YACGTR | 1 cut(s) | 126 |
| BstBI | TTCGAA | 1 cut(s) | 482 |
| BstC8I | GCNNGC | 1 cut(s) | 635 |
| BstDEI | CTNAG | 3 cut(s) | 530, 703, 743 |
| BstF5I | GGATG | 3 cut(s) | 248, 789, 819 |
| BstKTI | GATC | 4 cut(s) | 478, 517, 894, 1077 |
| BstMAI | GTCTC | 1 cut(s) | 1090 |
| BstMBI | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 41, 302, 581, 1019, 1028 |
| BstSCI | CCNGG | 1 cut(s) | 779 |
| BstSFI | CTRYAG | 1 cut(s) | 852 |
| BstSLI | GKGCMC | 1 cut(s) | 716 |
| BstSNI | TACGTA | 1 cut(s) | 126 |
| BstV1I | GCAGC | 4 cut(s) | 449, 546, 866, 896 |
| BstV2I | GAAGAC | 1 cut(s) | 594 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 154 |
| BsuI | GTATCC | 1 cut(s) | 908 |
| BtgZI | GCGATG | 1 cut(s) | 1052 |
| BtsCI | GGATG | 3 cut(s) | 248, 789, 819 |
| BtsIMutI | CAGTG | 4 cut(s) | 416, 590, 765, 1077 |
| Cac8I | GCNNGC | 1 cut(s) | 635 |
| CciI | TCATGA | 1 cut(s) | 910 |
| Cfr13I | GGNCC | 1 cut(s) | 839 |
| Csp6I | GTAC | 5 cut(s) | 262, 353, 407, 414, 495 |
| CviQI | GTAC | 5 cut(s) | 262, 353, 407, 414, 495 |
| DdeI | CTNAG | 3 cut(s) | 530, 703, 743 |
| DpnI | GATC | 4 cut(s) | 477, 516, 893, 1076 |
| DpnII | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| Eam1104I | CTCTTC | 1 cut(s) | 230 |
| EarI | CTCTTC | 1 cut(s) | 230 |
| Eco105I | TACGTA | 1 cut(s) | 126 |
| Eco130I | CCWWGG | 3 cut(s) | 206, 274, 925 |
| Eco47I | GGWCC | 1 cut(s) | 839 |
| EcoO109I | RGGNCCY | 1 cut(s) | 839 |
| EcoT14I | CCWWGG | 3 cut(s) | 206, 274, 925 |
| ErhI | CCWWGG | 3 cut(s) | 206, 274, 925 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 362, 394 |
| FaqI | GGGAC | 1 cut(s) | 852 |
| FbaI | TGATCA | 2 cut(s) | 475, 1074 |
| FblI | GTMKAC | 1 cut(s) | 190 |
| Fnu4HI | GCNGC | 4 cut(s) | 438, 535, 855, 885 |
| FokI | GGATG | 3 cut(s) | 235, 796, 826 |
| Fsp4HI | GCNGC | 4 cut(s) | 438, 535, 855, 885 |
| FspBI | CTAG | 3 cut(s) | 614, 717, 1019 |
| GluI | GCNGC | 4 cut(s) | 438, 535, 855, 885 |
| HapII | CCGG | 2 cut(s) | 696, 780 |
| HincII | GTYRAC | 2 cut(s) | 135, 319 |
| HindII | GTYRAC | 2 cut(s) | 135, 319 |
| HindIII | AAGCTT | 1 cut(s) | 931 |
| HinfI | GANTC | 5 cut(s) | 9, 279, 699, 1083, 1100 |
| HpaII | CCGG | 2 cut(s) | 696, 780 |
| HphI | GGTGA | 2 cut(s) | 385, 901 |
| Hpy166II | GTNNAC | 7 cut(s) | 135, 191, 319, 376, 416, 714, 849 |
| Hpy188I | TCNGA | 3 cut(s) | 744, 876, 1082 |
| Hpy188III | TCNNGA | 4 cut(s) | 82, 229, 696, 911 |
| Hpy8I | GTNNAC | 7 cut(s) | 135, 191, 319, 376, 416, 714, 849 |
| Hpy99I | CGWCG | 1 cut(s) | 17 |
| HpyAV | CCTTC | 4 cut(s) | 92, 364, 1036, 1068 |
| HpyCH4III | ACNGT | 4 cut(s) | 261, 562, 594, 769 |
| HpyCH4IV | ACGT | 1 cut(s) | 125 |
| HpyCH4V | TGCA | 7 cut(s) | 387, 437, 584, 619, 714, 854, 1000 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 41, 302, 581, 1019, 1028 |
| HpyF3I | CTNAG | 3 cut(s) | 530, 703, 743 |
| HpySE526I | ACGT | 1 cut(s) | 125 |
| Kpn2I | TCCGGA | 1 cut(s) | 695 |
| Ksp22I | TGATCA | 2 cut(s) | 475, 1074 |
| Kzo9I | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| LmnI | GCTCC | 3 cut(s) | 163, 1045, 1051 |
| Lsp1109I | GCAGC | 4 cut(s) | 449, 546, 866, 896 |
| LweI | GCATC | 1 cut(s) | 800 |
| MaeI | CTAG | 3 cut(s) | 614, 717, 1019 |
| MaeII | ACGT | 1 cut(s) | 125 |
| MaeIII | GTNAC | 2 cut(s) | 218, 334 |
| MalI | GATC | 4 cut(s) | 477, 516, 893, 1076 |
| MboI | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| MboII | GAAGA | 5 cut(s) | 142, 217, 594, 841, 965 |
| MhlI | GDGCHC | 1 cut(s) | 716 |
| MluCI | AATT | 4 cut(s) | 138, 162, 501, 957 |
| MlyI | GAGTC | 1 cut(s) | 1092 |
| MnlI | CCTC | 4 cut(s) | 233, 737, 738, 1074 |
| MroI | TCCGGA | 1 cut(s) | 695 |
| MroXI | GAANNNNTTC | 2 cut(s) | 8, 26 |
| MslI | CAYNNNNRTG | 4 cut(s) | 35, 421, 595, 770 |
| MspA1I | CMGCKG | 1 cut(s) | 887 |
| MspI | CCGG | 2 cut(s) | 696, 780 |
| MspR9I | CCNGG | 1 cut(s) | 781 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 41, 302, 581, 1019, 1028 |
| NciI | CCSGG | 1 cut(s) | 781 |
| NdeII | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| NlaIV | GGNNCC | 4 cut(s) | 159, 327, 685, 840 |
| NmuCI | GTSAC | 1 cut(s) | 334 |
| NspV | TTCGAA | 1 cut(s) | 482 |
| OliI | CACNNNNGTG | 1 cut(s) | 421 |
| PagI | TCATGA | 1 cut(s) | 910 |
| PdmI | GAANNNNTTC | 2 cut(s) | 8, 26 |
| PfeI | GAWTC | 4 cut(s) | 9, 279, 699, 1100 |
| PkrI | GCNGC | 4 cut(s) | 439, 536, 856, 886 |
| PleI | GAGTC | 1 cut(s) | 1091 |
| PpsI | GAGTC | 1 cut(s) | 1091 |
| Ppu21I | YACGTR | 1 cut(s) | 126 |
| PpuMI | RGGWCCY | 1 cut(s) | 839 |
| Psp5II | RGGWCCY | 1 cut(s) | 839 |
| PspN4I | GGNNCC | 4 cut(s) | 159, 327, 685, 840 |
| PspPI | GGNCC | 1 cut(s) | 839 |
| PspPPI | RGGWCCY | 1 cut(s) | 839 |
| PstI | CTGCAG | 1 cut(s) | 856 |
| RsaI | GTAC | 5 cut(s) | 263, 354, 408, 415, 496 |
| RsaNI | GTAC | 5 cut(s) | 262, 353, 407, 414, 495 |
| RseI | CAYNNNNRTG | 4 cut(s) | 35, 421, 595, 770 |
| SatI | GCNGC | 4 cut(s) | 438, 535, 855, 885 |
| Sau3AI | GATC | 4 cut(s) | 475, 514, 891, 1074 |
| Sau96I | GGNCC | 1 cut(s) | 839 |
| ScaI | AGTACT | 3 cut(s) | 263, 408, 496 |
| SchI | GAGTC | 1 cut(s) | 1092 |
| ScrFI | CCNGG | 1 cut(s) | 781 |
| SduI | GDGCHC | 1 cut(s) | 716 |
| SfaNI | GCATC | 1 cut(s) | 800 |
| SfcI | CTRYAG | 1 cut(s) | 852 |
| SfuI | TTCGAA | 1 cut(s) | 482 |
| SinI | GGWCC | 1 cut(s) | 839 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 35, 421, 595, 770 |
| SmlI | CTYRAG | 2 cut(s) | 80, 229 |
| SmoI | CTYRAG | 2 cut(s) | 80, 229 |
| SnaBI | TACGTA | 1 cut(s) | 126 |
| SpeI | ACTAGT | 1 cut(s) | 716 |
| Sse9I | AATT | 4 cut(s) | 138, 162, 501, 957 |
| SsiI | CCGC | 2 cut(s) | 393, 887 |
| SspI | AATATT | 1 cut(s) | 73 |
| SspMI | CTAG | 3 cut(s) | 614, 717, 1019 |
| StyD4I | CCNGG | 1 cut(s) | 779 |
| StyI | CCWWGG | 3 cut(s) | 206, 274, 925 |
| TaaI | ACNGT | 4 cut(s) | 261, 562, 594, 769 |
| TaiI | ACGT | 1 cut(s) | 128 |
| TaqI | TCGA | 3 cut(s) | 12, 143, 482 |
| TasI | AATT | 4 cut(s) | 138, 162, 501, 957 |
| TatI | WGTACW | 3 cut(s) | 261, 406, 494 |
| TfiI | GAWTC | 4 cut(s) | 9, 279, 699, 1100 |
| TscAI | CASTG | 4 cut(s) | 423, 597, 772, 1077 |
| TseFI | GTSAC | 1 cut(s) | 334 |
| TseI | GCWGC | 4 cut(s) | 437, 534, 854, 884 |
| Tsp45I | GTSAC | 1 cut(s) | 334 |
| TspGWI | ACGGA | 2 cut(s) | 721, 1078 |
| TspRI | CASTG | 4 cut(s) | 423, 597, 772, 1077 |
| VneI | GTGCAC | 1 cut(s) | 712 |
| VpaK11BI | GGWCC | 1 cut(s) | 839 |
| XmiI | GTMKAC | 1 cut(s) | 190 |
| XmnI | GAANNNNTTC | 2 cut(s) | 8, 26 |
| XspI | CTAG | 3 cut(s) | 614, 717, 1019 |
| ZrmI | AGTACT | 3 cut(s) | 263, 408, 496 |