Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0000665

Physical Location & Seq

Genomic Coordinates Reverse (-)

26731654 .. 26733668

Gene Structure 2015 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1125400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGCGGACGTGAAGGGGTGTGGCGAATGGAAGCCTTTTATTGCCATGGTAGCAATCGATTTCTCGTTCGCTATAGTGAATATTCTGCTCAAGAAAGTGCTCGATGAGGGAATGAACCATTTGGTTCTTATAACGTACCGGTTGACGATTGCTACCATTTTCTTAGCACCAGTTAGCTACTTTGGGGAAAGGAATACTAGACCAAAGCTCACAATTCGCATCACATGTTTTCTCTTCTTAAGTGCAATTCTTGGGACATCCCTAACACAATACTTCTTTCTCCTCGGAATCCAATACACATCTGCGACTTTTGCCTGTGCCTTCGTCAACATGGTGCCGGTGATCACTTTCATAATGGCGTTACCGTTCGGGTTAGAGAAAGTGAACTTAAAATGCCACAGCGGGAGAGCAAAAGTGCTCGGTACACTGGTGTGCGTCGGTGGTGCTTTTATACTAACTCTTTACAAAGGAGTTCCTCTATTTGATTCCTCAAAATCCACAGTTAGTACTCAAACCATAGATCATGGAATTCAGCTGAGGTCAACGCGAAACCATGGGGGATGGACGATTGGTTCTATAGCTTTGGTTGGCGGAACCCTATTGTGGTCTTCATGGTTTCTTGTTCAAACAAACATTAGCAAGATGTATCCTTGCCAGTATTCTAGCACCACAATAATGAATTTCTTTGGTGCCATTCAGTCTGCAATCCTTGGTGTTTGCACAGGAAGGAACCTTTCCATGTGGGTTCTGAGGGGAAGGACACAAGTCATAGCTGTCCTGTTTTCTGGAATGATAGCATCGGGATTAGGTTTTGTGGGGATGTCATGGTGTGTGAGGAAACGGGGGCCGGTGTTTACTGCAGCTTTTAGCCCGCTCGTGCAGATAATGGTGGCCCTAATTGGTATCCCCATCTTCCATGAACTACTCCACCTCGGAAGCTTGTTAGGATCTATCGTTGTGATCGTCGGCTTGTACATTCTTCTATGGGGTAAGAAAAAAGAGATGCAGAAATGTGCGACAAAACTAGTCCAAGAATCTGAAGCAATCAAAGAGCAGGAGCCGCATTTAGCCGCCATCACAGTCTCCGTTGATTCGCGGTGTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

369

Amino Acids

40.28

Weight (kDa)

9.51

Isoelectric Point (pI)

29.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	14 - 153	4.2e-16	EamA-like transporter family
EamA	PF00892	190 - 328	9.6e-08	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G30340 AT3G30340 AT4G01440 AT4G01440 AT4G01440 AT4G01440 AT4G01440
fragaria_vesca	FvH4_5g10300 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160
malus_domestica	MD00G1118800.v1.1 MD01G1095400.v1.1 MD01G1095700.v1.1 MD01G1097400.v1.1 MD06G1125400.v1.1 MD07G1164500.v1.1
prunus_persica	Prupe.2G203500_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1
pyrus_communis	pycom06g11570 pycom07g15960 pycom14g11730
rosa_chinensis	RchiOBHm_Chr1g0361761 RchiOBHm_Chr1g0361771 RchiOBHm_Chr7g0187931
rosa_laevigata	RLG00000004746 RLG00000027703 RLG00000027704
rosa_multiflora	Rmu_co8477061.1_g000001 Rmu_sc0015523.1_g000006 Rmu_sc0030043.1_g000001 Rmu_ssc0000124.1_g000022
rosa_roxburghii	Rroxscaffold_3G00266930 Rroxscaffold_4G00294710
rosa_rugosa	Rorug01G0291400 Rorug06G0485400 Rorug06G0485500
rosa_samantha	Rh1AG301800 Rh1AG301900 Rh1BG264600 Rh1BG264700 Rh1CG283100 Rh1CG283200 Rh1DG294600 Rh1DG294700 Rh7AG090600 Rh7BG092200 Rh7CG091300 Rh7DG093000
rosa_wichuraiana	Rw1G026740 Rw1G026750 Rw7G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	131
AccB1I	GGYRCC	2 cut(s)	334, 689
AccBSI	CCGCTC	1 cut(s)	874
AccII	CGCG	2 cut(s)	547, 1096
AciI	CCGC	7 cut(s)	5, 402, 591, 872, 1061, 1071, 1096
AclWI	GGATC	1 cut(s)	955
AcsI	RAATTY	2 cut(s)	528, 679
AcuI	CTGAAG	1 cut(s)	1059
AfaI	GTAC	4 cut(s)	137, 424, 508, 974
AfiI	CCNNNNNNNGG	1 cut(s)	603
AflII	CTTAAG	1 cut(s)	238
AflIII	ACRYGT	1 cut(s)	224
AgeI	ACCGGT	1 cut(s)	138
AgsI	TTSAA	1 cut(s)	626
AhlI	ACTAGT	1 cut(s)	1024
AjiI	CACGTC	1 cut(s)	10
AleI	CACNNNNGTG	1 cut(s)	430
AloI	GAACNNNNNNTCC	2 cut(s)	556, 588
AluBI	AGCT	7 cut(s)	177, 208, 535, 581, 773, 863, 939
AluI	AGCT	7 cut(s)	177, 208, 535, 581, 773, 863, 939
Alw21I	GWGCWC	2 cut(s)	102, 420
Alw26I	GTCTC	1 cut(s)	1087
AlwI	GGATC	1 cut(s)	955
AoxI	GGCC	2 cut(s)	845, 891
ApeKI	GCWGC	1 cut(s)	860
ApoI	RAATTY	2 cut(s)	528, 679
AsiGI	ACCGGT	1 cut(s)	138
AspS9I	GGNCC	2 cut(s)	845, 892
AsuHPI	GGTGA	1 cut(s)	352
BaeI	ACNNNNGTAYC	2 cut(s)	414, 447
BanI	GGYRCC	2 cut(s)	334, 689
BauI	CACGAG	1 cut(s)	875
BbsI	GAAGAC	1 cut(s)	600
Bbv12I	GWGCWC	2 cut(s)	102, 420
BbvCI	CCTCAGC	1 cut(s)	536
BbvI	GCAGC	1 cut(s)	872
BccI	CCATC	3 cut(s)	555, 917, 1082
BciVI	GTATCC	2 cut(s)	657, 914
BclI	TGATCA	1 cut(s)	342
BcoDI	GTCTC	1 cut(s)	1087
BcuI	ACTAGT	1 cut(s)	1024
BfaI	CTAG	3 cut(s)	198, 663, 1025
BfmI	CTRYAG	3 cut(s)	72, 576, 858
BfrI	CTTAAG	1 cut(s)	238
BfuI	GTATCC	2 cut(s)	657, 914
BisI	GCNGC	3 cut(s)	861, 1061, 1071
BlsI	GCNGC	3 cut(s)	862, 1062, 1072
BmcAI	AGTACT	1 cut(s)	508
BmgBI	CACGTC	1 cut(s)	10
BmgT120I	GGNCC	2 cut(s)	845, 892
BmiI	GGNNCC	6 cut(s)	336, 595, 691, 731, 846, 1059
BmsI	GCATC	3 cut(s)	228, 806, 993
BoxI	GACNNNNGTC	1 cut(s)	764
BpiI	GAAGAC	1 cut(s)	600
Bpu10I	CCTNAGC	1 cut(s)	536
BpuEI	CTTGAG	1 cut(s)	74
Bsa29I	ATCGAT	1 cut(s)	57
BsaJI	CCNNGG	5 cut(s)	45, 283, 553, 709, 931
BsaWI	WCCGGW	1 cut(s)	138
Bsc4I	CCNNNNNNNGG	1 cut(s)	603
Bse118I	RCCGGY	3 cut(s)	138, 337, 847
Bse1I	ACTGG	3 cut(s)	170, 432, 655
BseCI	ATCGAT	1 cut(s)	57
BseDI	CCNNGG	5 cut(s)	45, 283, 553, 709, 931
BseGI	GGATG	3 cut(s)	257, 566, 825
BseLI	CCNNNNNNNGG	1 cut(s)	603
BseMII	CTCAG	2 cut(s)	527, 740
BseNI	ACTGG	3 cut(s)	170, 432, 655
BseRI	GAGGAG	1 cut(s)	272
BseXI	GCAGC	1 cut(s)	872
BsgI	GTGCAG	1 cut(s)	899
Bsh1236I	CGCG	2 cut(s)	547, 1096
BshFI	GGCC	2 cut(s)	847, 893
BshNI	GGYRCC	2 cut(s)	334, 689
BshTI	ACCGGT	1 cut(s)	138
BshVI	ATCGAT	1 cut(s)	57
BsiHKAI	GWGCWC	2 cut(s)	102, 420
BsiSI	CCGG	3 cut(s)	139, 338, 848
BslFI	GGGAC	1 cut(s)	268
BslI	CCNNNNNNNGG	1 cut(s)	603
BsmAI	GTCTC	1 cut(s)	1087
BsmFI	GGGAC	1 cut(s)	268
BsnI	GGCC	2 cut(s)	847, 893
Bsp1286I	GDGCHC	2 cut(s)	102, 420
Bsp1407I	TGTACA	1 cut(s)	972
Bsp143I	GATC	4 cut(s)	342, 520, 947, 960
Bsp19I	CCATGG	2 cut(s)	45, 553
BspACI	CCGC	7 cut(s)	5, 402, 591, 872, 1061, 1071, 1096
BspANI	GGCC	2 cut(s)	847, 893
BspCNI	CTCAG	2 cut(s)	528, 741
BspDI	ATCGAT	1 cut(s)	57
BspFNI	CGCG	2 cut(s)	547, 1096
BspLI	GGNNCC	6 cut(s)	336, 595, 691, 731, 846, 1059
BspMAI	CTGCAG	1 cut(s)	862
BspPI	GGATC	1 cut(s)	955
BspT107I	GGYRCC	2 cut(s)	334, 689
BspTI	CTTAAG	1 cut(s)	238
BsrBI	CCGCTC	1 cut(s)	874
BsrFI	RCCGGY	3 cut(s)	138, 337, 847
BsrGI	TGTACA	1 cut(s)	972
BsrI	ACTGG	3 cut(s)	170, 432, 655
BssAI	RCCGGY	3 cut(s)	138, 337, 847
BssECI	CCNNGG	5 cut(s)	45, 283, 553, 709, 931
BssMI	GATC	4 cut(s)	342, 520, 947, 960
BssSI	CACGAG	1 cut(s)	875
BssT1I	CCWWGG	3 cut(s)	45, 553, 709
Bst2BI	CACGAG	1 cut(s)	875
Bst4CI	ACNGT	3 cut(s)	366, 502, 1081
Bst6I	CTCTTC	1 cut(s)	239
BstAFI	CTTAAG	1 cut(s)	238
BstAUI	TGTACA	1 cut(s)	972
BstC8I	GCNNGC	1 cut(s)	872
BstDEI	CTNAG	3 cut(s)	163, 536, 749
BstDSI	CCRYGG	2 cut(s)	45, 553
BstF5I	GGATG	3 cut(s)	257, 566, 825
BstFNI	CGCG	2 cut(s)	547, 1096
BstKTI	GATC	4 cut(s)	345, 523, 950, 963
BstMAI	GTCTC	1 cut(s)	1087
BstMBI	GATC	4 cut(s)	342, 520, 947, 960
BstMWI	GCNNNNNNNGC	3 cut(s)	50, 311, 1060
BstNSI	RCATGY	1 cut(s)	228
BstPAI	GACNNNNGTC	1 cut(s)	764
BstSFI	CTRYAG	3 cut(s)	72, 576, 858
BstUI	CGCG	2 cut(s)	547, 1096
BstV1I	GCAGC	1 cut(s)	872
BstV2I	GAAGAC	1 cut(s)	600
BstX2I	RGATCY	1 cut(s)	947
BstYI	RGATCY	1 cut(s)	947
Bsu15I	ATCGAT	1 cut(s)	57
BsuI	GTATCC	2 cut(s)	657, 914
BsuRI	GGCC	2 cut(s)	847, 893
BsuTUI	ATCGAT	1 cut(s)	57
BtgI	CCRYGG	2 cut(s)	45, 553
BtrI	CACGTC	1 cut(s)	10
BtsCI	GGATG	3 cut(s)	257, 566, 825
BtsIMutI	CAGTG	1 cut(s)	425
Cac8I	GCNNGC	1 cut(s)	872
Cfr10I	RCCGGY	3 cut(s)	138, 337, 847
Cfr13I	GGNCC	2 cut(s)	845, 892
ClaI	ATCGAT	1 cut(s)	57
CseI	GACGC	1 cut(s)	424
Csp6I	GTAC	4 cut(s)	136, 423, 507, 973
CspAI	ACCGGT	1 cut(s)	138
CviAII	CATG	9 cut(s)	46, 225, 331, 524, 554, 612, 739, 825, 917
CviQI	GTAC	4 cut(s)	136, 423, 507, 973
DdeI	CTNAG	3 cut(s)	163, 536, 749
DpnI	GATC	4 cut(s)	344, 522, 949, 962
DpnII	GATC	4 cut(s)	342, 520, 947, 960
Eam1104I	CTCTTC	1 cut(s)	239
EarI	CTCTTC	1 cut(s)	239
EciI	GGCGGA	2 cut(s)	20, 606
Eco130I	CCWWGG	3 cut(s)	45, 553, 709
Eco57I	CTGAAG	1 cut(s)	1059
EcoRI	GAATTC	1 cut(s)	528
EcoT14I	CCWWGG	3 cut(s)	45, 553, 709
ErhI	CCWWGG	3 cut(s)	45, 553, 709
FaeI	CATG	9 cut(s)	49, 228, 334, 527, 557, 615, 742, 828, 920
FaqI	GGGAC	1 cut(s)	268
FatI	CATG	9 cut(s)	45, 224, 330, 523, 553, 611, 738, 824, 916
FauI	CCCGC	2 cut(s)	395, 879
FbaI	TGATCA	1 cut(s)	342
Fnu4HI	GCNGC	3 cut(s)	861, 1061, 1071
FokI	GGATG	3 cut(s)	244, 573, 832
Fsp4HI	GCNGC	3 cut(s)	861, 1061, 1071
FspBI	CTAG	3 cut(s)	198, 663, 1025
GluI	GCNGC	3 cut(s)	861, 1061, 1071
HaeIII	GGCC	2 cut(s)	847, 893
HapII	CCGG	3 cut(s)	139, 338, 848
HgaI	GACGC	1 cut(s)	424
Hin1II	CATG	9 cut(s)	49, 228, 334, 527, 557, 615, 742, 828, 920
HincII	GTYRAC	3 cut(s)	144, 328, 543
HindII	GTYRAC	3 cut(s)	144, 328, 543
HindIII	AAGCTT	1 cut(s)	937
HinfI	GANTC	4 cut(s)	288, 485, 1034, 1091
HpaII	CCGG	3 cut(s)	139, 338, 848
HphI	GGTGA	1 cut(s)	352
Hpy166II	GTNNAC	6 cut(s)	144, 328, 385, 425, 543, 855
Hpy188I	TCNGA	4 cut(s)	287, 750, 935, 1039
Hpy188III	TCNNGA	3 cut(s)	91, 786, 801
Hpy8I	GTNNAC	6 cut(s)	144, 328, 385, 425, 543, 855
Hpy99I	CGWCG	2 cut(s)	440, 968
HpyAV	CCTTC	4 cut(s)	7, 331, 720, 750
HpyCH4III	ACNGT	3 cut(s)	366, 502, 1081
HpyCH4IV	ACGT	2 cut(s)	9, 134
HpyCH4V	TGCA	6 cut(s)	245, 704, 720, 860, 880, 1006
HpyF10VI	GCNNNNNNNGC	3 cut(s)	50, 311, 1060
HpyF3I	CTNAG	3 cut(s)	163, 536, 749
HpySE526I	ACGT	2 cut(s)	9, 134
Hsp92II	CATG	9 cut(s)	49, 228, 334, 527, 557, 615, 742, 828, 920
Ksp22I	TGATCA	1 cut(s)	342
Kzo9I	GATC	4 cut(s)	342, 520, 947, 960
LmnI	GCTCC	1 cut(s)	1057
Lsp1109I	GCAGC	1 cut(s)	872
LweI	GCATC	3 cut(s)	228, 806, 993
MaeI	CTAG	3 cut(s)	198, 663, 1025
MaeII	ACGT	2 cut(s)	9, 134
MaeIII	GTNAC	1 cut(s)	360
MalI	GATC	4 cut(s)	344, 522, 949, 962
MbiI	CCGCTC	1 cut(s)	874
MboI	GATC	4 cut(s)	342, 520, 947, 960
MboII	GAAGA	4 cut(s)	226, 600, 904, 971
MflI	RGATCY	1 cut(s)	947
MhlI	GDGCHC	2 cut(s)	102, 420
MluCI	AATT	5 cut(s)	213, 246, 528, 679, 897
MnlI	CCTC	8 cut(s)	100, 293, 486, 499, 531, 744, 828, 941
MseI	TTAA	2 cut(s)	239, 389
MslI	CAYNNNNRTG	2 cut(s)	430, 674
MspA1I	CMGCKG	2 cut(s)	402, 535
MspCI	CTTAAG	1 cut(s)	238
MspI	CCGG	3 cut(s)	139, 338, 848
MvnI	CGCG	2 cut(s)	547, 1096
MwoI	GCNNNNNNNGC	3 cut(s)	50, 311, 1060
NcoI	CCATGG	2 cut(s)	45, 553
NdeII	GATC	4 cut(s)	342, 520, 947, 960
NlaIII	CATG	9 cut(s)	49, 228, 334, 527, 557, 615, 742, 828, 920
NlaIV	GGNNCC	6 cut(s)	336, 595, 691, 731, 846, 1059
NspI	RCATGY	1 cut(s)	228
OliI	CACNNNNGTG	1 cut(s)	430
PciI	ACATGT	1 cut(s)	224
PcsI	WCGNNNNNNNCGW	1 cut(s)	960
PfeI	GAWTC	4 cut(s)	288, 485, 1034, 1091
PinAI	ACCGGT	1 cut(s)	138
PkrI	GCNGC	3 cut(s)	862, 1062, 1072
PscI	ACATGT	1 cut(s)	224
PshAI	GACNNNNGTC	1 cut(s)	764
PsiI	TTATAA	1 cut(s)	131
PspN4I	GGNNCC	6 cut(s)	336, 595, 691, 731, 846, 1059
PspPI	GGNCC	2 cut(s)	845, 892
PstI	CTGCAG	1 cut(s)	862
PsuI	RGATCY	1 cut(s)	947
PvuII	CAGCTG	1 cut(s)	535
RsaI	GTAC	4 cut(s)	137, 424, 508, 974
RsaNI	GTAC	4 cut(s)	136, 423, 507, 973
RseI	CAYNNNNRTG	2 cut(s)	430, 674
SaqAI	TTAA	2 cut(s)	239, 389
SatI	GCNGC	3 cut(s)	861, 1061, 1071
Sau3AI	GATC	4 cut(s)	342, 520, 947, 960
Sau96I	GGNCC	2 cut(s)	845, 892
ScaI	AGTACT	1 cut(s)	508
SduI	GDGCHC	2 cut(s)	102, 420
SfaNI	GCATC	3 cut(s)	228, 806, 993
SfcI	CTRYAG	3 cut(s)	72, 576, 858
SmiMI	CAYNNNNRTG	2 cut(s)	430, 674
SmlI	CTYRAG	2 cut(s)	89, 238
SmoI	CTYRAG	2 cut(s)	89, 238
SpeI	ACTAGT	1 cut(s)	1024
Sse9I	AATT	5 cut(s)	213, 246, 528, 679, 897
SsiI	CCGC	7 cut(s)	5, 402, 591, 872, 1061, 1071, 1096
SspI	AATATT	1 cut(s)	82
SspMI	CTAG	3 cut(s)	198, 663, 1025
StyI	CCWWGG	3 cut(s)	45, 553, 709
TaaI	ACNGT	3 cut(s)	366, 502, 1081
TaiI	ACGT	2 cut(s)	12, 137
TaqI	TCGA	2 cut(s)	57, 102
TasI	AATT	5 cut(s)	213, 246, 528, 679, 897
TatI	WGTACW	2 cut(s)	506, 972
TauI	GCSGC	2 cut(s)	1063, 1073
TfiI	GAWTC	4 cut(s)	288, 485, 1034, 1091
Tru1I	TTAA	2 cut(s)	239, 389
Tru9I	TTAA	2 cut(s)	239, 389
TscAI	CASTG	1 cut(s)	432
TseI	GCWGC	1 cut(s)	860
TspDTI	ATGAA	5 cut(s)	128, 340, 600, 692, 933
TspGWI	ACGGA	1 cut(s)	1075
TspRI	CASTG	1 cut(s)	432
Vha464I	CTTAAG	1 cut(s)	238
XapI	RAATTY	2 cut(s)	528, 679
XceI	RCATGY	1 cut(s)	228
XspI	CTAG	3 cut(s)	198, 663, 1025
ZrmI	AGTACT	1 cut(s)	508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.