Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000665

Physical Location & Seq

Genomic Coordinates Forward (+)

596397 .. 598752

Gene Structure 2356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G01440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGGTTACTGCGATGGTAAATGGACACCGGTGATTATTATGGTTATGATCAATTCAGCGCTGGGTTTAGCTAATGCTTTGGTTAAAAAAGTACTCGATGGTGGTGTTAACCATATGGTTATCGCAACATATCGTCTTGCTATTTCAACCCTATTTTTGGCACCAATCGCATTTTTTTGGGAACGGAAAACGAGACCGACACTAACGCTCAACATCTTGGTTCAGCTTTTCTTCAGTGCTCTTGTTGGGGCAAGTTTGACTCAGTATTTCTTCCTATTGGGGCTATCATACACATCGGCTACGTTGGCATGTGCTTTTATTAGCATGACGCCTGCGATTACATTCGTTATGGCTCTAATATTCAGGGTAGAGAAGCTAAACATGAAAAGCAAAGCAGGTATGGGGATGGTGATGGGCGCGTTGATATGCATAGGAGGAGCTTTATTATTAACAATGTACAAAGGTGTTCCCTTGACAAAACTTCGGAAACTAGAAACCCATCAGTTGATAAACAATAACCACGCTATGAAACCAGAGAATTGGATAATAGGATGCGTACTTCTCTTTGCGGGTAGTAGCTGTTTCGGGTCGTGGATGCTGATACAAGCCAAAGTGAACGAGAAATACCCTTGTCAATACTCGAGTACTGTCGTATTATCCTTCTTTGGCACCATCCAATGCGCCCTTCTAAGCCTCATCAAATCTCGAGATATCACGGCTTGGATCCTCACAGATAAACTTGATATCGTTACCATTGTTTACGCGGGAGCAGTAGCACAAGGAATATGTACGGTTGGAACGTCTTGGTGCATCAGAAAGAGAGGACCTATCTTTACTTCTATATTTACCCCGGTGGGGCTTATATTTGCAACACTATTTGATTTCTTGATCCTTCATCGTCAAATTTTCCTAGGAAGTGTTGTCGGATCTGGGGTTGTAATCTTCGGACTATACATATTCTTGTTGGGAAAAGTCAGGCTAATGAAAGAAGAGTGTGAAAAGAAGTTACCTTGTCGTTTTAATGAAGATGATCAAGAAGAAGATGATGATGAACAATACAAAAAGGGTCATCTTATGGTTGTTCCAATGACTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006855 GO:0006865 GO:0006868 GO:0007275 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0009791 GO:0010154 GO:0015075 GO:0015171 GO:0015175 GO:0015179 GO:0015186 GO:0015238 GO:0015318 GO:0015711 GO:0015804 GO:0015807 GO:0015849 GO:0015893 GO:0016020 GO:0022414 GO:0022857 GO:0032501 GO:0032502 GO:0034220 GO:0042221 GO:0042493 GO:0044464 GO:0046942 GO:0046943 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061458 GO:0071702 GO:0071705 GO:0071944 GO:0098655 GO:0098656 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

365

Amino Acids

40.44

Weight (kDa)

9.05

Isoelectric Point (pI)

22.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	13 - 144	1.4e-14	EamA-like transporter family
EamA	PF00892	183 - 321	2e-06	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G30340 AT3G30340 AT4G01440 AT4G01440 AT4G01440 AT4G01440 AT4G01440
fragaria_vesca	FvH4_5g10300 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160 FvH4_7g18160
malus_domestica	MD00G1118800.v1.1 MD01G1095400.v1.1 MD01G1095700.v1.1 MD01G1097400.v1.1 MD06G1125400.v1.1 MD07G1164500.v1.1
prunus_persica	Prupe.2G203500_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1 Prupe.5G135800_v2.0.a1
pyrus_communis	pycom06g11570 pycom07g15960 pycom14g11730
rosa_chinensis	RchiOBHm_Chr1g0361761 RchiOBHm_Chr1g0361771 RchiOBHm_Chr7g0187931
rosa_laevigata	RLG00000004746 RLG00000027703 RLG00000027704
rosa_multiflora	Rmu_co8477061.1_g000001 Rmu_sc0015523.1_g000006 Rmu_sc0030043.1_g000001 Rmu_ssc0000124.1_g000022
rosa_roxburghii	Rroxscaffold_3G00266930 Rroxscaffold_4G00294710
rosa_rugosa	Rorug01G0291400 Rorug06G0485400 Rorug06G0485500
rosa_samantha	Rh1AG301800 Rh1AG301900 Rh1BG264600 Rh1BG264700 Rh1CG283100 Rh1CG283200 Rh1DG294600 Rh1DG294700 Rh7AG090600 Rh7BG092200 Rh7CG091300 Rh7DG093000
rosa_wichuraiana	Rw1G026740 Rw1G026750 Rw7G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	386
AccB1I	GGYRCC	2 cut(s)	160, 668
AccII	CGCG	2 cut(s)	419, 764
AciI	CCGC	2 cut(s)	569, 764
AclWI	GGATC	4 cut(s)	718, 731, 883, 934
AcsI	RAATTY	1 cut(s)	903
AcuI	CTGAAG	1 cut(s)	217
AcyI	GRCGYC	1 cut(s)	329
AfaI	GTAC	5 cut(s)	93, 458, 558, 646, 790
AfeI	AGCGCT	1 cut(s)	60
AfiI	CCNNNNNNNGG	2 cut(s)	157, 855
AgeI	ACCGGT	1 cut(s)	28
AgsI	TTSAA	1 cut(s)	147
AjuI	GAANNNNNNNTTGG	2 cut(s)	669, 701
AluBI	AGCT	5 cut(s)	71, 226, 376, 440, 579
AluI	AGCT	5 cut(s)	71, 226, 376, 440, 579
Alw21I	GWGCWC	1 cut(s)	241
Alw26I	GTCTC	1 cut(s)	187
AlwI	GGATC	4 cut(s)	718, 731, 883, 934
Ama87I	CYCGRG	2 cut(s)	640, 705
Aor51HI	AGCGCT	1 cut(s)	60
ApoI	RAATTY	1 cut(s)	903
AsiGI	ACCGGT	1 cut(s)	28
AspA2I	CCTAGG	1 cut(s)	910
AspLEI	GCGC	3 cut(s)	61, 419, 683
AspS9I	GGNCC	1 cut(s)	824
AsuC2I	CCSGG	1 cut(s)	851
AsuHPI	GGTGA	2 cut(s)	43, 421
AvaI	CYCGRG	2 cut(s)	640, 705
AvaII	GGWCC	1 cut(s)	824
AvrII	CCTAGG	1 cut(s)	910
BamHI	GGATCC	1 cut(s)	723
BanI	GGYRCC	2 cut(s)	160, 668
Bbv12I	GWGCWC	1 cut(s)	241
BccI	CCATC	6 cut(s)	8, 92, 400, 406, 507, 680
BceAI	ACGGC	1 cut(s)	732
BclI	TGATCA	2 cut(s)	48, 1030
BcnI	CCSGG	1 cut(s)	851
BcoDI	GTCTC	1 cut(s)	187
BfaI	CTAG	2 cut(s)	491, 911
BfoI	RGCGCY	1 cut(s)	62
BfuAI	ACCTGC	1 cut(s)	386
BlnI	CCTAGG	1 cut(s)	910
BmcAI	AGTACT	2 cut(s)	93, 646
Bme1390I	CCNGG	1 cut(s)	851
Bme18I	GGWCC	1 cut(s)	824
BmeT110I	CYCGRG	2 cut(s)	640, 705
BmgT120I	GGNCC	1 cut(s)	824
BmiI	GGNNCC	3 cut(s)	162, 670, 725
BmrFI	CCNGG	1 cut(s)	851
BmsI	GCATC	3 cut(s)	542, 585, 819
BpuMI	CCSGG	1 cut(s)	851
BsaHI	GRCGYC	1 cut(s)	329
BsaI	GGTCTC	1 cut(s)	187
BsaJI	CCNNGG	2 cut(s)	849, 910
BsaWI	WCCGGW	1 cut(s)	28
Bsc4I	CCNNNNNNNGG	2 cut(s)	157, 855
Bse118I	RCCGGY	1 cut(s)	28
BseDI	CCNNGG	2 cut(s)	849, 910
BseGI	GGATG	4 cut(s)	411, 557, 600, 672
BseLI	CCNNNNNNNGG	2 cut(s)	157, 855
BseMII	CTCAG	1 cut(s)	275
BseRI	GAGGAG	1 cut(s)	450
BseYI	CCCAGC	1 cut(s)	61
Bsh1236I	CGCG	2 cut(s)	419, 764
BshNI	GGYRCC	2 cut(s)	160, 668
BshTI	ACCGGT	1 cut(s)	28
BsiHKAI	GWGCWC	1 cut(s)	241
BsiHKCI	CYCGRG	2 cut(s)	640, 705
BsiSI	CCGG	2 cut(s)	29, 851
BslI	CCNNNNNNNGG	2 cut(s)	157, 855
BsmAI	GTCTC	1 cut(s)	187
Bso31I	GGTCTC	1 cut(s)	187
BsoBI	CYCGRG	2 cut(s)	640, 705
Bsp1286I	GDGCHC	1 cut(s)	241
Bsp1407I	TGTACA	1 cut(s)	456
Bsp143I	GATC	5 cut(s)	48, 723, 888, 926, 1030
BspACI	CCGC	2 cut(s)	569, 764
BspCNI	CTCAG	1 cut(s)	274
BspFNI	CGCG	2 cut(s)	419, 764
BspLI	GGNNCC	3 cut(s)	162, 670, 725
BspMI	ACCTGC	1 cut(s)	386
BspPI	GGATC	4 cut(s)	718, 731, 883, 934
BspT107I	GGYRCC	2 cut(s)	160, 668
BspTNI	GGTCTC	1 cut(s)	187
BsrFI	RCCGGY	1 cut(s)	28
BsrGI	TGTACA	1 cut(s)	456
BssAI	RCCGGY	1 cut(s)	28
BssECI	CCNNGG	2 cut(s)	849, 910
BssMI	GATC	5 cut(s)	48, 723, 888, 926, 1030
BssNI	GRCGYC	1 cut(s)	329
BssT1I	CCWWGG	1 cut(s)	910
Bst4CI	ACNGT	2 cut(s)	649, 793
Bst6I	CTCTTC	1 cut(s)	984
BstACI	GRCGYC	1 cut(s)	329
BstAUI	TGTACA	1 cut(s)	456
BstC8I	GCNNGC	1 cut(s)	333
BstDEI	CTNAG	2 cut(s)	261, 689
BstF5I	GGATG	4 cut(s)	411, 557, 600, 672
BstFNI	CGCG	2 cut(s)	419, 764
BstH2I	RGCGCY	1 cut(s)	62
BstHHI	GCGC	3 cut(s)	61, 419, 683
BstKTI	GATC	5 cut(s)	51, 726, 891, 929, 1033
BstMAI	GTCTC	1 cut(s)	187
BstMBI	GATC	5 cut(s)	48, 723, 888, 926, 1030
BstMWI	GCNNNNNNNGC	2 cut(s)	167, 305
BstNSI	RCATGY	1 cut(s)	312
BstSCI	CCNGG	1 cut(s)	849
BstUI	CGCG	2 cut(s)	419, 764
BstX2I	RGATCY	2 cut(s)	723, 926
BstXI	CCANNNNNNTGG	1 cut(s)	540
BstYI	RGATCY	2 cut(s)	723, 926
BtgZI	GCGATG	1 cut(s)	27
BtsCI	GGATG	4 cut(s)	411, 557, 600, 672
BtsIMutI	CAGTG	1 cut(s)	241
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	1 cut(s)	333
CfoI	GCGC	3 cut(s)	61, 419, 683
Cfr10I	RCCGGY	1 cut(s)	28
Cfr13I	GGNCC	1 cut(s)	824
CseI	GACGC	1 cut(s)	337
Csp6I	GTAC	5 cut(s)	92, 457, 557, 645, 789
CspAI	ACCGGT	1 cut(s)	28
CviAII	CATG	3 cut(s)	309, 325, 382
CviQI	GTAC	5 cut(s)	92, 457, 557, 645, 789
DdeI	CTNAG	2 cut(s)	261, 689
DpnI	GATC	5 cut(s)	50, 725, 890, 928, 1032
DpnII	GATC	5 cut(s)	48, 723, 888, 926, 1030
Eam1104I	CTCTTC	1 cut(s)	984
EarI	CTCTTC	1 cut(s)	984
Eco130I	CCWWGG	1 cut(s)	910
Eco31I	GGTCTC	1 cut(s)	187
Eco32I	GATATC	2 cut(s)	712, 745
Eco47I	GGWCC	1 cut(s)	824
Eco47III	AGCGCT	1 cut(s)	60
Eco57I	CTGAAG	1 cut(s)	217
Eco88I	CYCGRG	2 cut(s)	640, 705
EcoO109I	RGGNCCY	1 cut(s)	824
EcoRV	GATATC	2 cut(s)	712, 745
EcoT14I	CCWWGG	1 cut(s)	910
EcoT22I	ATGCAT	1 cut(s)	431
ErhI	CCWWGG	1 cut(s)	910
FaeI	CATG	3 cut(s)	312, 328, 385
FatI	CATG	3 cut(s)	308, 324, 381
FauI	CCCGC	2 cut(s)	562, 757
FauNDI	CATATG	1 cut(s)	114
FbaI	TGATCA	2 cut(s)	48, 1030
FokI	GGATG	4 cut(s)	418, 564, 607, 659
FspBI	CTAG	2 cut(s)	491, 911
GlaI	GCGC	3 cut(s)	60, 418, 682
GsaI	CCCAGC	1 cut(s)	65
HaeII	RGCGCY	1 cut(s)	62
HapII	CCGG	2 cut(s)	29, 851
HgaI	GACGC	1 cut(s)	337
HhaI	GCGC	3 cut(s)	61, 419, 683
Hin1I	GRCGYC	1 cut(s)	329
Hin1II	CATG	3 cut(s)	312, 328, 385
Hin6I	GCGC	3 cut(s)	59, 417, 681
HinP1I	GCGC	3 cut(s)	59, 417, 681
HincII	GTYRAC	1 cut(s)	109
HindII	GTYRAC	1 cut(s)	109
HinfI	GANTC	2 cut(s)	259, 1090
HpaI	GTTAAC	1 cut(s)	109
HpaII	CCGG	2 cut(s)	29, 851
HphI	GGTGA	2 cut(s)	43, 421
Hpy166II	GTNNAC	3 cut(s)	109, 616, 760
Hpy188I	TCNGA	4 cut(s)	486, 815, 926, 947
Hpy188III	TCNNGA	4 cut(s)	705, 707, 886, 1034
Hpy8I	GTNNAC	3 cut(s)	109, 616, 760
HpyAV	CCTTC	3 cut(s)	670, 695, 902
HpyCH4III	ACNGT	2 cut(s)	649, 793
HpyCH4IV	ACGT	2 cut(s)	302, 800
HpyCH4V	TGCA	3 cut(s)	429, 810, 869
HpyF10VI	GCNNNNNNNGC	2 cut(s)	167, 305
HpyF3I	CTNAG	2 cut(s)	261, 689
HpySE526I	ACGT	2 cut(s)	302, 800
Hsp92I	GRCGYC	1 cut(s)	329
Hsp92II	CATG	3 cut(s)	312, 328, 385
HspAI	GCGC	3 cut(s)	59, 417, 681
Ksp22I	TGATCA	2 cut(s)	48, 1030
KspAI	GTTAAC	1 cut(s)	109
Kzo9I	GATC	5 cut(s)	48, 723, 888, 926, 1030
LmnI	GCTCC	2 cut(s)	437, 767
LpnPI	CCDG	9 cut(s)	42, 47, 345, 349, 381, 546, 864, 915, 961
LweI	GCATC	3 cut(s)	542, 585, 819
MaeI	CTAG	2 cut(s)	491, 911
MaeII	ACGT	2 cut(s)	302, 800
MaeIII	GTNAC	3 cut(s)	5, 748, 1005
MalI	GATC	5 cut(s)	50, 725, 890, 928, 1032
MboI	GATC	5 cut(s)	48, 723, 888, 926, 1030
MboII	GAAGA	7 cut(s)	223, 262, 934, 1001, 1037, 1049, 1052
MflI	RGATCY	2 cut(s)	723, 926
MhlI	GDGCHC	1 cut(s)	241
MluCI	AATT	3 cut(s)	52, 538, 903
MlyI	GAGTC	2 cut(s)	253, 1084
MmeI	TCCRAC	2 cut(s)	775, 904
MnlI	CCTC	4 cut(s)	428, 704, 737, 815
Mph1103I	ATGCAT	1 cut(s)	431
MseI	TTAA	4 cut(s)	84, 108, 449, 1020
MspI	CCGG	2 cut(s)	29, 851
MspR9I	CCNGG	1 cut(s)	851
MvnI	CGCG	2 cut(s)	419, 764
MwoI	GCNNNNNNNGC	2 cut(s)	167, 305
NciI	CCSGG	1 cut(s)	851
NdeI	CATATG	1 cut(s)	114
NdeII	GATC	5 cut(s)	48, 723, 888, 926, 1030
NlaIII	CATG	3 cut(s)	312, 328, 385
NlaIV	GGNNCC	3 cut(s)	162, 670, 725
NsiI	ATGCAT	1 cut(s)	431
NspI	RCATGY	1 cut(s)	312
PaeR7I	CTCGAG	2 cut(s)	640, 705
PcsI	WCGNNNNNNNCGW	1 cut(s)	797
PinAI	ACCGGT	1 cut(s)	28
PleI	GAGTC	2 cut(s)	253, 1084
PpsI	GAGTC	2 cut(s)	253, 1084
PpuMI	RGGWCCY	1 cut(s)	824
Psp5II	RGGWCCY	1 cut(s)	824
PspFI	CCCAGC	1 cut(s)	61
PspN4I	GGNNCC	3 cut(s)	162, 670, 725
PspPI	GGNCC	1 cut(s)	824
PspPPI	RGGWCCY	1 cut(s)	824
PspXI	VCTCGAGB	1 cut(s)	640
PsrI	GAACNNNNNNTAC	2 cut(s)	608, 640
PsuI	RGATCY	2 cut(s)	723, 926
RsaI	GTAC	5 cut(s)	93, 458, 558, 646, 790
RsaNI	GTAC	5 cut(s)	92, 457, 557, 645, 789
SaqAI	TTAA	4 cut(s)	84, 108, 449, 1020
Sau3AI	GATC	5 cut(s)	48, 723, 888, 926, 1030
Sau96I	GGNCC	1 cut(s)	824
ScaI	AGTACT	2 cut(s)	93, 646
SchI	GAGTC	2 cut(s)	253, 1084
ScrFI	CCNGG	1 cut(s)	851
SduI	GDGCHC	1 cut(s)	241
SfaNI	GCATC	3 cut(s)	542, 585, 819
Sfr274I	CTCGAG	2 cut(s)	640, 705
SgrAI	CRCCGGYG	1 cut(s)	28
SinI	GGWCC	1 cut(s)	824
SlaI	CTCGAG	2 cut(s)	640, 705
SmlI	CTYRAG	2 cut(s)	640, 705
SmoI	CTYRAG	2 cut(s)	640, 705
Sse9I	AATT	3 cut(s)	52, 538, 903
SsiI	CCGC	2 cut(s)	569, 764
SspI	AATATT	1 cut(s)	360
SspMI	CTAG	2 cut(s)	491, 911
StyD4I	CCNGG	1 cut(s)	849
StyI	CCWWGG	1 cut(s)	910
TaaI	ACNGT	2 cut(s)	649, 793
TaiI	ACGT	2 cut(s)	305, 803
TaqI	TCGA	3 cut(s)	96, 641, 706
TaqII	GACCGA	1 cut(s)	211
TasI	AATT	3 cut(s)	52, 538, 903
TatI	WGTACW	3 cut(s)	91, 456, 644
Tru1I	TTAA	4 cut(s)	84, 108, 449, 1020
Tru9I	TTAA	4 cut(s)	84, 108, 449, 1020
TscAI	CASTG	1 cut(s)	241
TspDTI	ATGAA	6 cut(s)	398, 542, 884, 998, 1038, 1065
TspGWI	ACGGA	1 cut(s)	199
TspRI	CASTG	1 cut(s)	241
VpaK11BI	GGWCC	1 cut(s)	824
XapI	RAATTY	1 cut(s)	903
XceI	RCATGY	1 cut(s)	312
XhoI	CTCGAG	2 cut(s)	640, 705
XmaJI	CCTAGG	1 cut(s)	910
XspI	CTAG	2 cut(s)	491, 911
ZrmI	AGTACT	2 cut(s)	93, 646
Zsp2I	ATGCAT	1 cut(s)	431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.